This study aimed to investigate the protective effects of herniarin against acrylamide neuro-toxicity. Rats were administered 50 mg/kg of acrylamide (Acr) along with oral doses of herniarin at 50, 100, and 200 mg/kg for 11 days. After treatment, the animals were sacrificed and biochemical markers including superoxide dismutase (SOD), catalase activity, malondialdehyde (MDA), nitric oxide (NO), thiols and acetylcholine esterase (AChE) level were evaluated. Moreover, mRNA expression of neuro-inflammatory cytokines including TNF-α, IL-1β, and IL-6 were measured using qRT-PCR method. As results, Acr increased MDA with subsequent reduction in SOD, catalase, and thiols content. In contrast, administration of herniarin remarkably normalised the antioxidants and decreased lipid peroxidation. Furthermore, it downregulated mRNA expression of TNF-α, IL-1β, and IL-6 (p < 0.001) as well as decreased NO (p < 0.01) and AchE levels (p < 0.001) in the Acr-injured brains. Herniarin ameliorated Acr-induced brain injury via modulating redox hemostasis, cholinergic function, as well as inhibiting neuro-inflammation in rats.
{"title":"Herniarin ameliorates acrylamide-induced neurotoxicity in rat: involvement of neuro-inflammation and acetylcholinesterase","authors":"Farshad Mirzavi , Arezoo Rajabian , Hossein Hosseini , Azar Hosseini","doi":"10.1080/14786419.2024.2415440","DOIUrl":"10.1080/14786419.2024.2415440","url":null,"abstract":"<div><div>This study aimed to investigate the protective effects of herniarin against acrylamide neuro-toxicity. Rats were administered 50 mg/kg of acrylamide (Acr) along with oral doses of herniarin at 50, 100, and 200 mg/kg for 11 days. After treatment, the animals were sacrificed and biochemical markers including superoxide dismutase (SOD), catalase activity, malondialdehyde (MDA), nitric oxide (NO), thiols and acetylcholine esterase (AChE) level were evaluated. Moreover, mRNA expression of neuro-inflammatory cytokines including TNF-α, IL-1β, and IL-6 were measured using qRT-PCR method. As results, Acr increased MDA with subsequent reduction in SOD, catalase, and thiols content. In contrast, administration of herniarin remarkably normalised the antioxidants and decreased lipid peroxidation. Furthermore, it downregulated mRNA expression of TNF-α, IL-1β, and IL-6 (<em>p</em> < 0.001) as well as decreased NO (<em>p</em> < 0.01) and AchE levels (<em>p</em> < 0.001) in the Acr-injured brains. Herniarin ameliorated Acr-induced brain injury <em>via</em> modulating redox hemostasis, cholinergic function, as well as inhibiting neuro-inflammation in rats.</div></div>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":"40 5","pages":"Pages 1161-1168"},"PeriodicalIF":1.6,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-04Epub Date: 2024-10-22DOI: 10.1080/14786419.2024.2420339
Chuanshen Gu , Zhenjun Chen , Xinyi Chen , Xuekang Su , Tingyi Xie , Zhen Nong , Chen Shen , Wenshu Luo , Fuxia Yang
This study used network pharmacology, bioinformatics, molecular docking and molecular dynamics simulations to evaluate the pharmacological mechanism of Jinshui Liujun decoction in treating chronic bronchitis. The network pharmacology constructed and analysed the drug-disease targets network. Microarray data analysis revealed CTSD as a key therapeutic target gene. Molecular docking and molecular dynamics simulations results indicated CTSD, can bind with β-sitosterol, cerevisterol, stigmasterol effectively. This study identifies the mechanism of action and gene expression of the key therapeutic target CTSD of Jinshui Liujun decoction in the treatment of chronic bronchitis, and verifies its binding stability with the key active components, aiming to provide new targets and research directions for the clinical treatment of chronic bronchitis.
{"title":"CTSD is the key therapeutic target of Jinshui Liujun decoction in treating chronic bronchitis","authors":"Chuanshen Gu , Zhenjun Chen , Xinyi Chen , Xuekang Su , Tingyi Xie , Zhen Nong , Chen Shen , Wenshu Luo , Fuxia Yang","doi":"10.1080/14786419.2024.2420339","DOIUrl":"10.1080/14786419.2024.2420339","url":null,"abstract":"<div><div>This study used network pharmacology, bioinformatics, molecular docking and molecular dynamics simulations to evaluate the pharmacological mechanism of Jinshui Liujun decoction in treating chronic bronchitis. The network pharmacology constructed and analysed the drug-disease targets network. Microarray data analysis revealed CTSD as a key therapeutic target gene. Molecular docking and molecular dynamics simulations results indicated CTSD, can bind with β-sitosterol, cerevisterol, stigmasterol effectively. This study identifies the mechanism of action and gene expression of the key therapeutic target CTSD of Jinshui Liujun decoction in the treatment of chronic bronchitis, and verifies its binding stability with the key active components, aiming to provide new targets and research directions for the clinical treatment of chronic bronchitis.</div></div>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":"40 5","pages":"Pages 1234-1237"},"PeriodicalIF":1.6,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study provides novel findings on the ameliorative effect of hyperoside isolated from Marsdenia latifolia leaves (HIGLL) in reproductive health challenged by manganese toxicity. The study investigated the efficacy of HIGLL on male fertility in rats exposed to manganese chloride (MnCl2). The rats received either MnCl2 (30 mg/L) or in combination with HIGLL (100 mg/kg or 200 mg/kg). MnCl2 reduced fluid intake, organ-body weight, body weight; sperm count, sperm viability, sperm density, sperm motility, semen viscosity, daily sperm production, testicular sperm number; testosterone, follicle-stimulating and luteinising hormones, oestradiol, testosterone/oestradiol ratio; G6PDH, ALP, glucose, NO, acid phosphatase, sialic acid, 17-β-HSD, superoxide dismutase, CAT, GST, GSH, and T-SH without modifying the semen pH and volume, while it correspondingly raised the abnormalities associated with morphology of sperm cells in head, neck, and tail; H2O2 and lipid peroxidation levels. HIGLL abrogated the damaged histoarchitecture of the testes and epididymis caused by MnCl2. HIGLL can serve as a therapeutic agent in the management of male reproductive disorders related to oxidative stress and endocrine disruption.
{"title":"Ethnopharmacological insights: hyperoside from Marsdenia latifolia (Benth.) K.Schum. mitigates reproductive toxicity in male Wistar rats induced by manganese exposure","authors":"Olalekan Bukunmi Ogunro , Anthonia Oluyemi Agboola , Folake Olubukola Asejeje , Gideon Ampoma Gyebi","doi":"10.1080/14786419.2024.2423041","DOIUrl":"10.1080/14786419.2024.2423041","url":null,"abstract":"<div><div>This study provides novel findings on the ameliorative effect of hyperoside isolated from <em>Marsdenia latifolia</em> leaves (HIGLL) in reproductive health challenged by manganese toxicity. The study investigated the efficacy of HIGLL on male fertility in rats exposed to manganese chloride (MnCl<sub>2</sub>). The rats received either MnCl<sub>2</sub> (30 mg/L) or in combination with HIGLL (100 mg/kg or 200 mg/kg). MnCl<sub>2</sub> reduced fluid intake, organ-body weight, body weight; sperm count, sperm viability, sperm density, sperm motility, semen viscosity, daily sperm production, testicular sperm number; testosterone, follicle-stimulating and luteinising hormones, oestradiol, testosterone/oestradiol ratio; G6PDH, ALP, glucose, NO, acid phosphatase, sialic acid, 17-β-HSD, superoxide dismutase, CAT, GST, GSH, and T-SH without modifying the semen pH and volume, while it correspondingly raised the abnormalities associated with morphology of sperm cells in head, neck, and tail; H<sub>2</sub>O<sub>2</sub> and lipid peroxidation levels. HIGLL abrogated the damaged histoarchitecture of the testes and epididymis caused by MnCl<sub>2</sub>. HIGLL can serve as a therapeutic agent in the management of male reproductive disorders related to oxidative stress and endocrine disruption.</div></div>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":"40 5","pages":"Pages 1265-1276"},"PeriodicalIF":1.6,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142562462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-04Epub Date: 2024-10-30DOI: 10.1080/14786419.2024.2424390
Mohan Lal , Dipankar Sutradhar
Tribulus terrestris L., a medicinal plant rich in secondary metabolites, was studied for optimising bioactive compound extraction from various parts of the plant using ethanol–water (50:50), ethanol, and methanol solvents. Analysis of extracts for key phytochemicals like polyphenols, flavonoids, saponins, and alkaloids was performed using HPTLC, HPLC and gas chromatography. The ethanol–water mixture proved best for extracting saponins and polyphenols, ethanol for flavonoids, and methanol for alkaloids. The fruit yielded the highest saponin content (59.34% ± 3.87), while leaves were richest in polyphenols (18.94% ± 1.39), flavonoids (5.15% ± 1.01), and alkaloids (26.46% ± 1.71). Leaf extracts showed the highest antibacterial activity against B. subtilis and P. aeruginosa, and stem extracts were effective against E. coli. Root, stem, and leaf extracts exhibited antifungal activity with leaf extract also demonstrating strong phytoestrogenic activity. These findings highlight the varied phytochemical profiles and biological activities of T. terrestris, suggesting their potential therapeutic uses.
{"title":"A comprehensive analysis of phytochemicals, antioxidant, anti-inflammatory, antibacterial, antifungal and phytoestrogenic properties of different parts of Tribulus terrestris","authors":"Mohan Lal , Dipankar Sutradhar","doi":"10.1080/14786419.2024.2424390","DOIUrl":"10.1080/14786419.2024.2424390","url":null,"abstract":"<div><div><em>Tribulus terrestris</em> L., a medicinal plant rich in secondary metabolites, was studied for optimising bioactive compound extraction from various parts of the plant using ethanol–water (50:50), ethanol, and methanol solvents. Analysis of extracts for key phytochemicals like polyphenols, flavonoids, saponins, and alkaloids was performed using HPTLC, HPLC and gas chromatography. The ethanol–water mixture proved best for extracting saponins and polyphenols, ethanol for flavonoids, and methanol for alkaloids. The fruit yielded the highest saponin content (59.34% ± 3.87), while leaves were richest in polyphenols (18.94% ± 1.39), flavonoids (5.15% ± 1.01), and alkaloids (26.46% ± 1.71). Leaf extracts showed the highest antibacterial activity against <em>B. subtilis</em> and <em>P. aeruginosa</em>, and stem extracts were effective against <em>E. coli</em>. Root, stem, and leaf extracts exhibited antifungal activity with leaf extract also demonstrating strong phytoestrogenic activity. These findings highlight the varied phytochemical profiles and biological activities of <em>T. terrestris</em>, suggesting their potential therapeutic uses.</div></div>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":"40 5","pages":"Pages 1295-1301"},"PeriodicalIF":1.6,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cisplatin is a chemotherapeutic agent known for causing severe peripheral neuropathy as a side effect, impacting patients’ quality of life by damaging nerve tissues. This study aims to explore the neuroprotective effects of the ethanolic extract of Alpinia calcarata Roscoe rhizome (EEACR) and stigmasterol identified by high-performance liquid chromatography (HPLC) in a rat model of cisplatin-induced neuropathy. Male Wistar rats were divided into control, cisplatin-induced neuropathic, and two intervention groups receiving different concentrations of EEACR (250 and 500 mg/kg). Neuropathy was induced with cisplatin administered intraperitoneally at a dose of 2 mg/kg per week for five weeks. The intervention groups were treated orally with EEACR daily during the induction period. Treatment with EEACR showed a significant attenuation of neurotoxicity as evidenced by behavioural improvements in mechanical allodynia, thermal hyperalgesia, and cold allodynia. HPLC analysis confirmed the presence of stigmasterol in EEACR, which may contribute to its therapeutic effects. Biochemical assessments revealed a significant decrease in oxidative stress markers and an increase in antioxidant enzyme activities, including superoxide dismutase, catalase, and glutathione peroxidase, in the nervous tissues of EEACR-treated rats. Histopathological examination indicated a reduction in nerve damage and enhanced preservation of myelin and axonal structures in the treatment groups. The findings from this study suggest that EEACR, enriched with stigmasterol, offers promising neuroprotective effects against cisplatin-induced neuropathy in rats.
{"title":"Neuroprotective potential of Alpinia calcarata and HPLC analysis of stigmasterol against cisplatin-induced neuropathy in rat model","authors":"Apurwa Dhavale , Hariprasad M.G , Pallavi Chiprikar","doi":"10.1080/14786419.2024.2426201","DOIUrl":"10.1080/14786419.2024.2426201","url":null,"abstract":"<div><div>Cisplatin is a chemotherapeutic agent known for causing severe peripheral neuropathy as a side effect, impacting patients’ quality of life by damaging nerve tissues. This study aims to explore the neuroprotective effects of the ethanolic extract of <em>Alpinia calcarata</em> Roscoe rhizome (EEACR) and stigmasterol identified by high-performance liquid chromatography (HPLC) in a rat model of cisplatin-induced neuropathy. Male Wistar rats were divided into control, cisplatin-induced neuropathic, and two intervention groups receiving different concentrations of EEACR (250 and 500 mg/kg). Neuropathy was induced with cisplatin administered intraperitoneally at a dose of 2 mg/kg per week for five weeks. The intervention groups were treated orally with EEACR daily during the induction period. Treatment with EEACR showed a significant attenuation of neurotoxicity as evidenced by behavioural improvements in mechanical allodynia, thermal hyperalgesia, and cold allodynia. HPLC analysis confirmed the presence of stigmasterol in EEACR, which may contribute to its therapeutic effects. Biochemical assessments revealed a significant decrease in oxidative stress markers and an increase in antioxidant enzyme activities, including superoxide dismutase, catalase, and glutathione peroxidase, in the nervous tissues of EEACR-treated rats. Histopathological examination indicated a reduction in nerve damage and enhanced preservation of myelin and axonal structures in the treatment groups. The findings from this study suggest that EEACR, enriched with stigmasterol, offers promising neuroprotective effects against cisplatin-induced neuropathy in rats.</div></div>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":"40 5","pages":"Pages 1354-1362"},"PeriodicalIF":1.6,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-04Epub Date: 2024-10-12DOI: 10.1080/14786419.2024.2416506
Xinyu Zhou , Aiping Xu , Xinmei Chen
Coreopsis tinctoria (C. tinctoria) is a traditional plant with high medicinal value and edible value. It has the effect of invigorating spleen and stomach and has the potential to develop into flower tea. Therefore, the extraction process of C. tinctoria extract (CTA) was optimised by analytic hierarchy process (AHP) combined with orthogonal test, and its activity was predicted by network pharmacology and verified by experiments. The optimal extraction conditions: temperature of 80 °C, extraction period of 10 min, solid-liquid ratio of 1:70 and times 2. CTA had no gastrointestinal irritation and can significantly increase the faecal water content of mice, inhibit amylase activity, increase protease, xylanase and cellulase activity, increase the number of intestinal Lactobacillus and Bifidobacterium, reduce the number of Escherichia coli and Enterococcus. In summary, CTA can effectively improve the intestinal digestive enzyme activity and the number of probiotics and has the potential to treat gastrointestinal diseases.
{"title":"Extraction, pharmacological prediction and experimental verification of Coreopsis tinctoria flowers","authors":"Xinyu Zhou , Aiping Xu , Xinmei Chen","doi":"10.1080/14786419.2024.2416506","DOIUrl":"10.1080/14786419.2024.2416506","url":null,"abstract":"<div><div><em>Coreopsis tinctoria</em> (<em>C. tinctoria</em>) is a traditional plant with high medicinal value and edible value. It has the effect of invigorating spleen and stomach and has the potential to develop into flower tea. Therefore, the extraction process of <em>C. tinctoria</em> extract (CTA) was optimised by analytic hierarchy process (AHP) combined with orthogonal test, and its activity was predicted by network pharmacology and verified by experiments. The optimal extraction conditions: temperature of 80 °C, extraction period of 10 min, solid-liquid ratio of 1:70 and times 2. CTA had no gastrointestinal irritation and can significantly increase the faecal water content of mice, inhibit amylase activity, increase protease, xylanase and cellulase activity, increase the number of intestinal <em>Lactobacillus</em> and <em>Bifidobacterium</em>, reduce the number of <em>Escherichia coli</em> and <em>Enterococcus</em>. In summary, CTA can effectively improve the intestinal digestive enzyme activity and the number of probiotics and has the potential to treat gastrointestinal diseases.</div></div>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":"40 5","pages":"Pages 1369-1374"},"PeriodicalIF":1.6,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Strawberry is one of the most commonly eaten berries. Breeding procedures led to the separation of two types of strawberries: June – bearing and Ever-bearing strawberries fruit for about a month and Ever – bearing throughout the growing season. The aim of the project is to compare the antioxidant parameters between both types of strawberries with those of two other dicots (blackberry and red raspberry). The research covered 29 strawberry clones: 10 Ever – bearing and 19 June – bearing, 6 blackberries, and 12 red raspberry. Statistical analysis of the Mann-Whitney U test showed that in terms of the tested parameters there are no statistical differences between the two types of strawberries; however, the year-round yield and extended availability of fresh fruit mean that these varieties are gaining more and more interest from producers and consumers. Repeat-fruiting strawberries are statistically more than twice as efficient than traditional varieties, not inferior in taste to traditional varieties.
{"title":"Antioxidant activity of new strawberry clones: June-bearing and ever-bearing","authors":"Joanna Jagła , Klaudia Dynarowicz , David Aebisher , Alicja Krośniak , Mirosław Krośniak , Kamil Jurowski","doi":"10.1080/14786419.2024.2422522","DOIUrl":"10.1080/14786419.2024.2422522","url":null,"abstract":"<div><div>Strawberry is one of the most commonly eaten berries. Breeding procedures led to the separation of two types of strawberries: June – bearing and Ever-bearing strawberries fruit for about a month and Ever – bearing throughout the growing season. The aim of the project is to compare the antioxidant parameters between both types of strawberries with those of two other dicots (blackberry and red raspberry). The research covered 29 strawberry clones: 10 Ever – bearing and 19 June – bearing, 6 blackberries, and 12 red raspberry. Statistical analysis of the Mann-Whitney <em>U</em> test showed that in terms of the tested parameters there are no statistical differences between the two types of strawberries; however, the year-round yield and extended availability of fresh fruit mean that these varieties are gaining more and more interest from producers and consumers. Repeat-fruiting strawberries are statistically more than twice as efficient than traditional varieties, not inferior in taste to traditional varieties.</div></div>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":"40 5","pages":"Pages 1261-1264"},"PeriodicalIF":1.6,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-04Epub Date: 2024-11-03DOI: 10.1080/14786419.2024.2426062
Karthikeyan Kandaswamy , Ajay Guru
{"title":"The potential role of lumazine peptide penilumamide E from Aspergillus terreus in head and neck cancer treatment","authors":"Karthikeyan Kandaswamy , Ajay Guru","doi":"10.1080/14786419.2024.2426062","DOIUrl":"10.1080/14786419.2024.2426062","url":null,"abstract":"","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":"40 5","pages":"Pages 1392-1393"},"PeriodicalIF":1.6,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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