Pub Date : 2026-01-02DOI: 10.1080/14786419.2024.2345756
Thi Thuy Duong Ngo , Ngoc Phuc Bui , Thi Kieu Loan Vo , Thi My Nuong Nguyen , Hoang Khang Le , Thanh Tung Phan , Poul Erik Hansen , Quang Ton That
Previous results from the our research group have isolated numerous compounds, including novel ones, but the anticancer activity of Miliusa velutina has not been demonstrated. In this study, from the most active ethyl acetate extract of the stems of Miliusa velutina, seven compounds were isolated and determined structures, including a new drimane sesquiterpenoid compound named miliutine C methyl ester (1) and three bioactive alkaloids (5-7). These three alkaloids (5-7) exhibited strong anticancer activities against various cancer cell lines such as MCF-7, HepG2, HeLa, NCI H460 and normal fibroblasts. Especially, on MCF-7 and normal fibroblasts with values of IC50 (μM) in order for compounds 5 (3.38, 31.15), 6 (21.96, 102.00), 7 (7.90, greater than 300), to compare with positive control camptothecin (0.020, 4.51); which is highly noteworthy. These results contribute to elucidating and confirming the value of Miliusa velutina, similar to other published and folkloric findings.
{"title":"Miliutine C methyl ester, a new drimane sesquiterpene and bioactive alkaloids from the stems of Miliusa velutina","authors":"Thi Thuy Duong Ngo , Ngoc Phuc Bui , Thi Kieu Loan Vo , Thi My Nuong Nguyen , Hoang Khang Le , Thanh Tung Phan , Poul Erik Hansen , Quang Ton That","doi":"10.1080/14786419.2024.2345756","DOIUrl":"10.1080/14786419.2024.2345756","url":null,"abstract":"<div><div>Previous results from the our research group have isolated numerous compounds, including novel ones, but the anticancer activity of <em>Miliusa velutina</em> has not been demonstrated. In this study, from the most active ethyl acetate extract of the stems of <em>Miliusa velutina</em>, seven compounds were isolated and determined structures, including a new drimane sesquiterpenoid compound named miliutine C methyl ester (<strong>1</strong>) and three bioactive alkaloids (<strong>5</strong>-<strong>7</strong>). These three alkaloids (<strong>5</strong>-<strong>7</strong>) exhibited strong anticancer activities against various cancer cell lines such as MCF-7, HepG2, HeLa, NCI H460 and normal fibroblasts. Especially, on MCF-7 and normal fibroblasts with values of IC<sub>50</sub> (μM) in order for compounds <strong>5</strong> (3.38, 31.15), <strong>6</strong> (21.96, 102.00), <strong>7</strong> (7.90, greater than 300), to compare with positive control camptothecin (0.020, 4.51); which is highly noteworthy. These results contribute to elucidating and confirming the value of <em>Miliusa velutina</em>, similar to other published and folkloric findings.</div></div>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":"40 1","pages":"Pages 1-7"},"PeriodicalIF":1.6,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140665858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-02DOI: 10.1080/14786419.2024.2446710
Hailin Long , Mengzhen Liu , Shanyue Guan , Xiaoting Huang , Zhongchen Rao , Li Cao , Richou Han
As part of our plan to discover new bioactive ingredients from entomophagous fungi, a new macrolide compound, talarolide (1), and a new bioactive natural product, talarenal (2), with two known compounds, WF-3681 methy ester (3) and aspergillumarin A (4) were isolated from an insect derived strain Talaromyces rugulosus GIZ45-A37. The structures were determined based on extensive comprehensive spectroscopic (NMR and HR-ESI-MS) analyses and compared with literature values. The relative configuration of 1 was defined by DFT-NMR chemical shift calculations and subsequent DP4/DP4+ probability methods. Compounds 1 and 2 showed inhibitory effect on IL-6 production at 10 and 20 μM in LPS-stimulated beas-2b cells, indicating their potential anti-inflammatory activity.
{"title":"Bioactive secondary metabolites from Talaromyces rugulosus GIZ45-A37 and their anti-inflammatory activity","authors":"Hailin Long , Mengzhen Liu , Shanyue Guan , Xiaoting Huang , Zhongchen Rao , Li Cao , Richou Han","doi":"10.1080/14786419.2024.2446710","DOIUrl":"10.1080/14786419.2024.2446710","url":null,"abstract":"<div><div>As part of our plan to discover new bioactive ingredients from entomophagous fungi, a new macrolide compound, talarolide (1), and a new bioactive natural product, talarenal (2), with two known compounds, WF-3681 methy ester (3) and aspergillumarin A (4) were isolated from an insect derived strain <em>Talaromyces rugulosus</em> GIZ45-A37. The structures were determined based on extensive comprehensive spectroscopic (NMR and HR-ESI-MS) analyses and compared with literature values. The relative configuration of 1 was defined by DFT-NMR chemical shift calculations and subsequent DP4/DP4+ probability methods. Compounds 1 and 2 showed inhibitory effect on IL-6 production at 10 and 20 μM in LPS-stimulated beas-2b cells, indicating their potential anti-inflammatory activity.</div></div>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":"40 1","pages":"Pages 266-271"},"PeriodicalIF":1.6,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-02DOI: 10.1080/14786419.2024.2380013
Zouhour Jebli , Hajer Riguene , Amira Yahyaoui , Soumaya Hassni , Souad Dali , Ridha Ben Salem , Ghayth Rigane
A new ultrasound-assisted extraction method was developed for the determination of phenolic compounds extracted from Passiflora edulis Sims peels cultivated in Tunisia. Several extraction variables including: extraction time, temperature, liquid/solid ratio and pH have been studied using response surface methodology. The extraction efficiency was evaluated by measuring the total phenolics; flavonoids and antioxidants content. The highest values of the studied response were observed after 9.78 min at 49.64 °C in a liquid-to-solid ratio 22.07 ml/g and in pH (5.54). The individual phenolic compounds content of the optimum extract peels of the Passiflora edulis Sims have been analysed using HPLC-DAD. The results revealed the presence of gallic followed by caffeic acids, while the apigenin-7-glucoside and rutin have been quantified as the abundant flavonoid compounds (0.653 and 0.488 mg/ml, respectively). This green procedure should be a promising option to guide industrial design for the production of phenolic-rich plant extracts.
{"title":"Ultrasound-assisted extraction, optimisation using response surface methodology and HPLC-DAD phenolic compounds quantification from Passiflora edulis S. peels cultivated in Tunisia","authors":"Zouhour Jebli , Hajer Riguene , Amira Yahyaoui , Soumaya Hassni , Souad Dali , Ridha Ben Salem , Ghayth Rigane","doi":"10.1080/14786419.2024.2380013","DOIUrl":"10.1080/14786419.2024.2380013","url":null,"abstract":"<div><div>A new ultrasound-assisted extraction method was developed for the determination of phenolic compounds extracted from <em>Passiflora edulis Sims</em> peels cultivated in Tunisia. Several extraction variables including: extraction time, temperature, liquid/solid ratio and pH have been studied using response surface methodology. The extraction efficiency was evaluated by measuring the total phenolics; flavonoids and antioxidants content. The highest values of the studied response were observed after 9.78 min at 49.64 °C in a liquid-to-solid ratio 22.07 ml/g and in pH (5.54). The individual phenolic compounds content of the optimum extract peels of the <em>Passiflora edulis</em> Sims have been analysed using HPLC-DAD. The results revealed the presence of gallic followed by caffeic acids, while the apigenin-7-glucoside and rutin have been quantified as the abundant flavonoid compounds (0.653 and 0.488 mg/ml, respectively). This green procedure should be a promising option to guide industrial design for the production of phenolic-rich plant extracts.</div></div>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":"40 1","pages":"Pages 13-21"},"PeriodicalIF":1.6,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141727543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-02DOI: 10.1080/14786419.2024.2383994
Hong-Tao Li , Xing-Yi Guo , Ya-Ting Shao , Wen-Yu Huang , Cui-Ping Miao , Wei Li
A new phthalide derivative named paramlyktone (1) and a new arborinane-type triterpenoid named paramyrpenoid (2), together with ten previously described trichothecenes derivatives (3–12) were isolated and identified from a rhizospheric soil-derived Paramyrothecium sp. KMU22107 associated with Delphinium yunnanense. Their structural elucidation was achieved by the comprehensive analysis of spectroscopic data and comparison with literature values. Notably, paramyrpenoid (2) was the first example of an arborinane-type triterpenoid with a double bond at Δ12(13) and an additional methyl motif at C-8. This was the first report of arborinane-type triterpenoids from a fungus belonging to Paramyrothecium genus. In pharmacological studies, paramyrpenoid (2) demonstrated significant cytotoxic activity against the HL-60, SW480, A-549, MDA-MB-231 and SMMC-7721 cell lines, with IC50 values from 2.0 to 16.1 μM. Compounds 1 and 2 were also evaluated for anti-inflammatory, anti-acetylcholinesterase (AChE), and protein tyrosine phosphatase 1B (PTP1B) inhibitory activities in vitro.
{"title":"Triterpenoid and trichothecenes from a rhizospheric soil-derived Paramyrothecium sp. KMU22107","authors":"Hong-Tao Li , Xing-Yi Guo , Ya-Ting Shao , Wen-Yu Huang , Cui-Ping Miao , Wei Li","doi":"10.1080/14786419.2024.2383994","DOIUrl":"10.1080/14786419.2024.2383994","url":null,"abstract":"<div><div>A new phthalide derivative named paramlyktone (<strong>1</strong>) and a new arborinane-type triterpenoid named paramyrpenoid (<strong>2</strong>), together with ten previously described trichothecenes derivatives (<strong>3–12</strong>) were isolated and identified from a rhizospheric soil-derived <em>Paramyrothecium</em> sp. KMU22107 associated with <em>Delphinium yunnanense</em>. Their structural elucidation was achieved by the comprehensive analysis of spectroscopic data and comparison with literature values. Notably, paramyrpenoid (<strong>2</strong>) was the first example of an arborinane-type triterpenoid with a double bond at Δ<sup>12(13)</sup> and an additional methyl motif at C-8. This was the first report of arborinane-type triterpenoids from a fungus belonging to <em>Paramyrothecium</em> genus. In pharmacological studies, paramyrpenoid (<strong>2</strong>) demonstrated significant cytotoxic activity against the HL-60, SW480, A-549, MDA-MB-231 and SMMC-7721 cell lines, with IC<sub>50</sub> values from 2.0 to 16.1 μM. Compounds <strong>1</strong> and <strong>2</strong> were also evaluated for anti-inflammatory, anti-acetylcholinesterase (AChE), and protein tyrosine phosphatase 1B (PTP1B) inhibitory activities <em>in vitro</em>.</div></div>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":"40 1","pages":"Pages 57-66"},"PeriodicalIF":1.6,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-02DOI: 10.1080/14786419.2024.2397044
Yong Zhang , You-Di Rao , Jing-Su Yu , Jia-Yi Hu , Wen-Hui Hu , Shu-Ri Li , Hui Yang , Yan-Ping Liu , Yan-Hui Fu
A phytochemical study on the stems and leaves of Cratoxylum cochinchinense (Lour.) Blume resulted in the isolation and characterisation of a new polyprenylated xanthone, cratocochinone (1), as well as seven known analogues, fuscaxanthone K (2), pruniflorone Q (3), 1,3,5,8-tetrahy-droxy- 2-(3-methybut-2-enyl)-4-(3,7-dimethylocta-2,6-dienyl) xanthone (4), cochinensoxanthone (5), cratoxylum-xanthone B (6), cochinchinone I (7) and cochinchinone K (8). The chemical structure of 1 was determined by comprehensive spectral analyses. The known compounds 2 − 8 were identified by comparing their experimental spectroscopic data with those reported data in the literature. The anti-inflammatory and anti-HIV effects of all isolates 1–8 were evaluated. As a result, compounds 1–8 showed remarkable inhibitory effects against nitric oxide (NO) production induced by lipopolysaccharide in mouse macrophage RAW 264.7 cells showing IC50 values ranging from 0.68 ± 0.06 to 10.27 ± 0.18 μM. Meanwhile, compounds 1–8 displayed notable anti-HIV-1 reverse transcriptase (RT) effects with EC50 values ranging from 0.19 to 10.72 µM.
{"title":"Polyprenylated xanthones with potential anti-inflammatory and anti-HIV effects from the stems and leaves of Cratoxylum cochinchinense (Lour.) Blume","authors":"Yong Zhang , You-Di Rao , Jing-Su Yu , Jia-Yi Hu , Wen-Hui Hu , Shu-Ri Li , Hui Yang , Yan-Ping Liu , Yan-Hui Fu","doi":"10.1080/14786419.2024.2397044","DOIUrl":"10.1080/14786419.2024.2397044","url":null,"abstract":"<div><div>A phytochemical study on the stems and leaves of <em>Cratoxylum cochinchinense</em> (Lour.) Blume resulted in the isolation and characterisation of a new polyprenylated xanthone, cratocochinone (<strong>1</strong>), as well as seven known analogues, fuscaxanthone K (<strong>2</strong>), pruniflorone Q (<strong>3</strong>), 1,3,5,8-tetrahy-droxy- 2-(3-methybut-2-enyl)-4-(3,7-dimethylocta-2,6-dienyl) xanthone (<strong>4</strong>), cochinensoxanthone (<strong>5</strong>), cratoxylum-xanthone B (<strong>6</strong>), cochinchinone I (<strong>7</strong>) and cochinchinone K (<strong>8</strong>). The chemical structure of <strong>1</strong> was determined by comprehensive spectral analyses. The known compounds <strong>2 </strong>−<strong> 8</strong> were identified by comparing their experimental spectroscopic data with those reported data in the literature. The anti-inflammatory and anti-HIV effects of all isolates <strong>1</strong>–<strong>8</strong> were evaluated. As a result, compounds <strong>1</strong>–<strong>8</strong> showed remarkable inhibitory effects against nitric oxide (NO) production induced by lipopolysaccharide in mouse macrophage RAW 264.7 cells showing IC<sub>50</sub> values ranging from 0.68 ± 0.06 to 10.27 ± 0.18 μM. Meanwhile, compounds <strong>1</strong>–<strong>8</strong> displayed notable anti-HIV-1 reverse transcriptase (RT) effects with EC<sub>50</sub> values ranging from 0.19 to 10.72 µM.</div></div>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":"40 1","pages":"Pages 172-180"},"PeriodicalIF":1.6,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142126222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The healthy properties of bee pollen mainly depend on the botanical, geographical origin and storage conditions. This study aimed to characterise the composition of volatile compounds and describe the sensory profile of Tuscan bee pollens collected in two different areas in the same period and stored for six months under different conditions. The headspace solid-phase microextraction (HS-SPME) analyses of volatile compounds showed that non-terpene derivatives represented the predominant chemical class in all examined samples, among which aldehydes were the main compounds. Apocaroteonids and oxygenated monoterpenes were also present in lower percentages, with strong separation of the chemical profiles between room temperature samples and those of other storage conditions. The sensory profile was analysed by panel test, the bee pollen produced in the suburban area seemed to show less attractiveness, taste and olfactory. Both samples stored at room temperature showed the worst sensory profile, compared to the other storage conditions.
{"title":"The volatile and sensory profiles of Tuscan bee pollens stored at different temperatures","authors":"Sonia Capparelli , Ylenia Pieracci , Simona Sagona , Guido Flamini , Francesca Coppola , Chiara Sanmartin , Isabella Taglieri , Francesca Venturi , Antonio Felicioli , Laura Pistelli","doi":"10.1080/14786419.2024.2389312","DOIUrl":"10.1080/14786419.2024.2389312","url":null,"abstract":"<div><div>The healthy properties of bee pollen mainly depend on the botanical, geographical origin and storage conditions. This study aimed to characterise the composition of volatile compounds and describe the sensory profile of Tuscan bee pollens collected in two different areas in the same period and stored for six months under different conditions. The headspace solid-phase microextraction (HS-SPME) analyses of volatile compounds showed that non-terpene derivatives represented the predominant chemical class in all examined samples, among which aldehydes were the main compounds. Apocaroteonids and oxygenated monoterpenes were also present in lower percentages, with strong separation of the chemical profiles between room temperature samples and those of other storage conditions. The sensory profile was analysed by panel test, the bee pollen produced in the suburban area seemed to show less attractiveness, taste and olfactory. Both samples stored at room temperature showed the worst sensory profile, compared to the other storage conditions.</div></div>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":"40 1","pages":"Pages 114-121"},"PeriodicalIF":1.6,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-02DOI: 10.1080/14786419.2024.2394834
Lu-Ting Dai , Li Yang , Zi-Peng Wang , Jiao-Cen Guo , Qing-Yun Ma , Qing-Yi Xie , Hao-Fu Dai , Zhi-Fang Yu , You-Xing Zhao
A new steroid named persteroid (1) and seven known compounds (2–8) were isolated from the marine-derived fungus Penicillium sp. ZYX-Z-143. The structure of 1 was determined by HRESIMS, NMR, and ECD calculations. Compound 1 showed inhibitory activity against protein tyrosine phosphatase 1B (PTP1B) with IC50 value of 46.31 ± 0.52 μM. Moreover, compound 1 potently suppressed nitric oxide (NO) production on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. The cytotoxicity and antibacterial activity of all isolates were tested.
{"title":"Persteroid, a new steroid from the marine-derived fungus Penicillium sp. ZYX-Z-143","authors":"Lu-Ting Dai , Li Yang , Zi-Peng Wang , Jiao-Cen Guo , Qing-Yun Ma , Qing-Yi Xie , Hao-Fu Dai , Zhi-Fang Yu , You-Xing Zhao","doi":"10.1080/14786419.2024.2394834","DOIUrl":"10.1080/14786419.2024.2394834","url":null,"abstract":"<div><div>A new steroid named persteroid (<strong>1</strong>) and seven known compounds (<strong>2</strong>–<strong>8</strong>) were isolated from the marine-derived fungus <em>Penicillium</em> sp. ZYX-Z-143. The structure of <strong>1</strong> was determined by HRESIMS, NMR, and ECD calculations. Compound <strong>1</strong> showed inhibitory activity against protein tyrosine phosphatase 1B (PTP1B) with IC<sub>50</sub> value of 46.31 ± 0.52 μM. Moreover, compound <strong>1</strong> potently suppressed nitric oxide (NO) production on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. The cytotoxicity and antibacterial activity of all isolates were tested.</div></div>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":"40 1","pages":"Pages 164-171"},"PeriodicalIF":1.6,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-02DOI: 10.1080/14786419.2024.2384081
Ari Sri Windyaswari , Media Fitri Isma Nugraha , Rika Hartati , Elfahmi
The Pothos genus is extensively utilised in traditional medicine in China and India. An underexplored species of Pothos tener Wall was identified in Sulawesi, Indonesia. Antimicrobial activity was assessed using microdilutions and streak plates against Staphylococcus aureus, Eschericia coli, Aeromonas hydrophila, Aspergillus niger, and Candida albicans. Significant effectiveness was observed in the methanol extract, as indicated by the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values for three different extracts (methanol, ethyl acetate, and n-hexane) of P. tener. The isolates obtained were structurally analysed using Ultraviolet (UV)-spectroscopy, Fourier-transform Infra Red-Spectroscopy (FT-IR), Mass Spectroscopy (MS), Nuclear Magnetic Resonance (NMR), and antimicrobial testing after undergoing fractionation and subfractionation. The isolate obtained was stigmasterol with moderate antimicrobial activity against A. niger and A. hydrophila, with MIC equivalent to MBC of 500 µg/ml. The first report of stigmasterol from P. tener has potent antimicrobial properties, bolstering empirical data in this field.
Pothos 属植物在中国和印度被广泛用于传统医药。在印度尼西亚苏拉威西岛发现了一种未被充分开发的 Pothos tener Wall 物种。使用微量稀释法和条纹平板对金黄色葡萄球菌、大肠杆菌、嗜水气单胞菌、黑曲霉和白色念珠菌的抗菌活性进行了评估。从 P. tener 的三种不同提取物（甲醇、乙酸乙酯和正己烷）的最低抑菌浓度 (MIC) 和最低杀菌浓度 (MBC) 值可以看出，甲醇提取物具有显著的效果。对获得的分离物进行了结构分析，使用了紫外光谱（UV）、傅立叶变换红外光谱（FT-IR）、质谱（MS）、核磁共振（NMR），并在分馏和再分馏后进行了抗菌测试。分离得到的豆甾醇对黑曲霉和蚜虫具有中等程度的抗菌活性，其 MIC 相当于 500 µg/ml 的 MBC。这是首次报道从 P. tener 中提取的豆固醇具有强效抗菌特性，从而加强了该领域的经验数据。
{"title":"Isolation and antimicrobial activity of secondary metabolites of pothos tener wall","authors":"Ari Sri Windyaswari , Media Fitri Isma Nugraha , Rika Hartati , Elfahmi","doi":"10.1080/14786419.2024.2384081","DOIUrl":"10.1080/14786419.2024.2384081","url":null,"abstract":"<div><div>The <em>Pothos</em> genus is extensively utilised in traditional medicine in China and India. An underexplored species of <em>Pothos tener</em> Wall was identified in Sulawesi, Indonesia. Antimicrobial activity was assessed using microdilutions and streak plates against <em>Staphylococcus aureus</em>, <em>Eschericia coli, Aeromonas hydrophila</em>, <em>Aspergillus niger</em>, and <em>Candida albicans</em>. Significant effectiveness was observed in the methanol extract, as indicated by the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values for three different extracts (methanol, ethyl acetate, and <em>n</em>-hexane) of <em>P. tener</em>. The isolates obtained were structurally analysed using Ultraviolet (UV)-spectroscopy, Fourier-transform Infra Red-Spectroscopy (FT-IR), Mass Spectroscopy (MS), Nuclear Magnetic Resonance (NMR), and antimicrobial testing after undergoing fractionation and subfractionation. The isolate obtained was stigmasterol with moderate antimicrobial activity against <em>A. niger</em> and <em>A. hydrophila</em>, with MIC equivalent to MBC of 500 µg/ml. The first report of stigmasterol from <em>P. tener</em> has potent antimicrobial properties, bolstering empirical data in this field.</div></div>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":"40 1","pages":"Pages 73-81"},"PeriodicalIF":1.6,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141893863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-02DOI: 10.1080/14786419.2024.2381017
Dabolé Bernard , Matcheme Matthieu , Gade Isaac Silvère , Moussa Djaouda , Nyemb Jean Noël , Moïse Diguir , Saram Clément , Talla Emmanuel , Sophie Laurent , Céline Henoumont , Alessandro Venditti , Atchadé Alex De Theodore , Loura Benoît
The phytochemical study of Cordia myxa L. led to the isolation, through chromatographic techniques, of a new triterpenoid saponin, 3-O-[α-L-rhamnopyranosyl-(1→3)-(6-O-acetyl-β-D-glucopyranosyl)]-22β-hydroxyolean-12-ene (3) namely Myxaoside A, together with three known compounds, Soyasaponine I (1), oleanolic acid (2), and 3-O-acetyl-oleanolic acid (4). All structures were established, based on 1 & 2D-NMR spectroscopic analysis and comparison with previous published reports. Compound 1-4 were evaluated for their antibacterial activity on various strains of bacteria including Salmonella typhi, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae and Vibrio cholerae. It appears that compounds 1 and 3 were active on all the tested microbial species, while compounds 2 and 4, shown no significant effect on S. aureus and K. pneumoniae at low concentrations 6.5 mg/mL and 3.0 mg/mL.
{"title":"Myxaoside A, a new triterpenoid saponin from Cordia myxa L. (Boraginaceae)","authors":"Dabolé Bernard , Matcheme Matthieu , Gade Isaac Silvère , Moussa Djaouda , Nyemb Jean Noël , Moïse Diguir , Saram Clément , Talla Emmanuel , Sophie Laurent , Céline Henoumont , Alessandro Venditti , Atchadé Alex De Theodore , Loura Benoît","doi":"10.1080/14786419.2024.2381017","DOIUrl":"10.1080/14786419.2024.2381017","url":null,"abstract":"<div><div>The phytochemical study of <em>Cordia myxa</em> L. led to the isolation, through chromatographic techniques, of a new triterpenoid saponin, 3-<em>O</em>-[<em>α</em>-<em>L</em>-rhamnopyranosyl-(1→3)-(6-<em>O</em>-acetyl-<em>β</em>-<em>D</em>-glucopyranosyl)]-22<em>β</em>-hydroxyolean-12-ene (<strong>3</strong>) namely Myxaoside A, together with three known compounds, Soyasaponine I (<strong>1</strong>), oleanolic acid (<strong>2</strong>), and 3-<em>O</em>-acetyl-oleanolic acid (<strong>4</strong>). All structures were established, based on 1 & 2D-NMR spectroscopic analysis and comparison with previous published reports. Compound <strong>1</strong>-<strong>4</strong> were evaluated for their antibacterial activity on various strains of bacteria including <em>Salmonella typhi, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae</em> and <em>Vibrio cholerae</em>. It appears that compounds <strong>1</strong> and <strong>3</strong> were active on all the tested microbial species, while compounds <strong>2</strong> and <strong>4</strong>, shown no significant effect on <em>S. aureus</em> and <em>K. pneumoniae</em> at low concentrations 6.5 mg/mL and 3.0 mg/mL.</div></div>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":"40 1","pages":"Pages 22-27"},"PeriodicalIF":1.6,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141875375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lichen is well-known for its various purposes. However, understanding the chemical composition and antimicrobial characteristics of Graphis cf. handelii remains insufficient. In this study, a new compound, graphinone A (1), together with three known compounds, handelone (2), 4-O-methylhiascic acid (3), and ethyl orsellinate (4) were isolated and structurally elucidated. Their chemical structures were established using comprehensive spectroscopic data (1D- and 2D-NMR and HRESIMS). Compounds 1–4 were tested for antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and evaluated for the alpha-glucosidase inhibition.
{"title":"A new diphenyl ether from the cultured lichen mycobiont of Graphis cf. handelii","authors":"Thanh-Hung Do , Thuc-Huy Duong , Minh-Truong-Tho Ho , Duc-Dung Pham , Thu-Hoang-Mai Nguyen , Chanat Aonbangkhen , Jirapast Sichaem , Chuong Hoang Nguyen , Thi-Phi-Giao Vo , Ngoc-Hong Nguyen , Thi-Minh-Dinh Tran","doi":"10.1080/14786419.2024.2398736","DOIUrl":"10.1080/14786419.2024.2398736","url":null,"abstract":"<div><div>Lichen is well-known for its various purposes. However, understanding the chemical composition and antimicrobial characteristics of <em>Graphis</em> cf. <em>handelii</em> remains insufficient. In this study, a new compound, graphinone A (<strong>1</strong>), together with three known compounds, handelone (<strong>2</strong>), 4-<em>O</em>-methylhiascic acid (<strong>3</strong>), and ethyl orsellinate (<strong>4</strong>) were isolated and structurally elucidated. Their chemical structures were established using comprehensive spectroscopic data (1D- and 2D-NMR and HRESIMS). Compounds <strong>1</strong>–<strong>4</strong> were tested for antimicrobial activity against methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) and evaluated for the alpha-glucosidase inhibition.</div></div>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":"40 1","pages":"Pages 189-195"},"PeriodicalIF":1.6,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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