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Identification of printed circuit boards mechanical properties using response surface methods 用响应面法识别印刷电路板的机械性能
IF 1.1 4区 工程技术 Q3 Engineering Pub Date : 2021-12-03 DOI: 10.1108/mi-09-2021-0085
Mohammad A. Gharaibeh
PurposeThis study aims to discuss the determination of the unknown in-plane mechanical material properties of printed circuit boards (PCBs) by correlating the results from dynamic testing and finite element (FE) models using the response surface method (RSM).Design/methodology/approachThe first 10 resonant frequencies and vibratory mode shapes are measured using modal analysis with hammer testing experiment, and hence, systematically compared with finite element analysis (FEA) results. The RSM is consequently used to minimize the cumulative error between dynamic testing and FEA results by continuously modifying the FE model, to acquire material properties of PCBs.FindingsGreat agreement is shown when comparing FEA to measurements, the optimum in-plane material properties were identified, and hence, verified.Originality/valueThis paper used FEA and RSMs along with modal measurements to obtain in-plane material properties of PCBs. The methodology presented here can be easily generalized and repeated for different board designs and configurations.
目的本研究旨在讨论通过使用响应面法(RSM)将动态测试结果与有限元(FE)模型相关联来确定印刷电路板(PCB)的未知平面内机械材料特性。设计/方法/方法使用模态分析和锤击测试来测量前10个谐振频率和振型实验,并因此与有限元分析(FEA)结果进行了系统的比较。因此,通过不断修改有限元模型,RSM被用于最大限度地减少动态测试和有限元分析结果之间的累积误差,以获得PCB的材料特性。发现当将有限元分析与测量结果进行比较时,显示出极大的一致性,从而确定并验证了最佳平面内材料特性。独创性/价值本文使用有限元分析和RSM以及模态测量来获得PCB的平面内材料特性。这里提出的方法可以很容易地推广和重复不同的板设计和配置。
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引用次数: 0
Improvement of inter layer dielectric crack for LQFP C90FG wafer technology devices in copper wire bonding process LQFP C90FG晶圆工艺器件在铜线键合过程中层间介电裂纹的改进
IF 1.1 4区 工程技术 Q3 Engineering Pub Date : 2021-11-23 DOI: 10.1108/mi-07-2021-0059
Xiuqiang Wu, D. Ye, Hanmin Zhang, Li Song, L. Guo
PurposeThis paper aims to investigate the root causes of and implement the improvements for the inter layer dielectric (ILD) crack for LQFP C90FG (CMOS90 Floating Gate) wafer technology devices in copper wire bonding process.Design/methodology/approachFailure analysis was conducted including cratering, scanning electron microscopy inspection and focus ion beam cross-section analysis, which showed ILD crack. Root cause investigation of ILD crack rate sudden jumping was carried out with cause-and-effect analysis, which revealed the root cause is shallower lead frame down-set. ILD crack mechanism deep-dive on ILD crack due to shallower lead frame down-set, which revealed the mechanism is lead frame flag floating on heat insert. Further investigation and energy dispersive X-ray analysis found the Cu particles on heat insert is another factor that can result in lead frame flag floating.FindingsLead frame flag floating on heat insert caused by shallower lead frame down-set or foreign matter on heat insert is a critical factor of ILD crack that has never been revealed before. Weak wafer structure strength caused by thinner wafer passivation1 thickness and sharp corner at Metal Trench (compared with the benchmarking fab) are other factors that can impact ILD crack.Originality/valueFor ILD crack improvement in copper wire bonding, besides the obvious factors such as wafer structure and wire bonding parameters, also should take other factors into consideration including lead frame flag floating on heat insert and heat insert maintenance.
目的研究LQFP C90FG (CMOS90浮栅)晶圆工艺器件在铜线键合过程中产生层间介电(ILD)裂纹的根本原因并实施改进措施。设计/方法/方法进行了失效分析,包括凹坑、扫描电镜检查和聚焦离子束截面分析,结果显示为ILD裂纹。通过因果分析,对ILD裂纹率突然跳变的根本原因进行了调查,发现其根本原因是引线架下陷较浅。由于引线框架下陷较浅,引线框架下陷较深,导致引线框架下陷较深,表明引线框架标志在热嵌件上浮动。进一步的调查和能量色散x射线分析发现,热插片上的Cu颗粒是导致引线框架旗漂浮的另一个因素。发现引线架下陷较浅或引线架上有异物引起的引线架旗在热嵌件上的浮动是造成ILD裂纹的关键因素,这是以往从未发现过的。与基准晶圆厂相比,晶圆钝化厚度较薄导致的晶圆结构强度较弱以及Metal Trench的尖角是影响ILD裂纹的其他因素。原创性/价值对于铜线焊接的ILD裂纹改善,除了考虑晶片结构、焊线参数等明显因素外,还应考虑热插片上引线框旗的浮动、热插片的维护等因素。
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引用次数: 1
A novel miniaturized Koch-Minkowski hybrid fractal antenna 一种新型微型化科赫-闵可夫斯基混合分形天线
IF 1.1 4区 工程技术 Q3 Engineering Pub Date : 2021-11-17 DOI: 10.1108/mi-07-2021-0069
Zain ul Abidin Jaffri, Zeeshan Ahmad, Asif Kabir, S. Bukhari
PurposeAntenna miniaturization, multiband operation and wider operational bandwidth are vital to achieve optimal design for modern wireless communication devices. Using fractal geometries is recognized as one of the most promising solutions to attain these characteristics. The purpose of this paper is to present a unique structure of patch antenna using hybrid fractal technique to enhance the performance characteristics for various wireless applications and to achieve better miniaturization.Design/methodology/approachIn this paper, the authors propose a novel hybrid fractal antenna by combining Koch and Minkowski (K-M) fractal geometries. A microstrip patch antenna (MPA) operating at 1.8 GHz is incorporated with a novel K-M hybrid fractal geometry. The proposed fractal antenna is designed and simulated in CST Microwave studio and compared with existing Koch fractal geometry. The prototype for the third iteration of the K-M fractal antenna is then fabricated on FR-4 substrate and tested through vector network analyzer for operating band/voltage standing wave ratio.FindingsThe third iteration of the proposed K-M fractal geometry results in achieving a 20% size reduction as compared to an ordinary MPA for the same resonant frequency with impedance bandwidth of 16.25 MHz and a directional gain of 6.48 dB, respectively. The operating frequency of MPA also lowers down to 1.44 GHz.Originality/valueFurther testing for the radiation patterns in an anechoic chamber shows good agreement to those of simulated results.
目的天线小型化、多频带操作和更宽的操作带宽对于实现现代无线通信设备的优化设计至关重要。使用分形几何被认为是实现这些特性的最有前途的解决方案之一。本文的目的是利用混合分形技术提出一种独特的贴片天线结构,以增强各种无线应用的性能特性,并实现更好的小型化。设计/方法/途径本文将Koch和Minkowski(K-M)分形几何相结合,提出了一种新型的混合分形天线。一种工作于1.8的微带贴片天线(MPA) GHz与一种新的K-M混合分形几何相结合。在CST微波工作室对所提出的分形天线进行了设计和仿真,并与现有的Koch分形几何进行了比较。然后在FR-4衬底上制作了K-M分形天线第三次迭代的原型,并通过矢量网络分析仪对其工作频带/电压驻波比进行了测试。发现对于阻抗带宽为16.25的相同谐振频率,所提出的K-M分形几何的第三次迭代导致与普通MPA相比,尺寸减小了20% MHz和6.48的定向增益 dB。MPA的工作频率也降至1.44 GHz。原始性/值在消声室中对辐射模式的进一步测试显示出与模拟结果的良好一致性。
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引用次数: 1
Influence of selected factors on parameters of a cooling system with a Peltier module and forced air flow 选定因素对带珀尔帖模块和强制气流的冷却系统参数的影响
IF 1.1 4区 工程技术 Q3 Engineering Pub Date : 2021-11-16 DOI: 10.1108/mi-07-2021-0058
K. Posobkiewicz, K. Górecki
PurposeThe purpose of this study is to investigate the validation of the usefulness of cooling systems containing Peltier modules for cooling power devices based on measurements of the influence of selected factors on the value of thermal resistance of such a cooling system.Design/methodology/approachA cooling system containing a heat-sink, a Peltier module and a fan was built by the authors and the measurements of temperatures and thermal resistance in various supply conditions of the Peltier module and the fan were carried out and discussed.FindingsConclusions from the research carried out answer the question if the use of Peltier modules in active cooling systems provides any benefits comparing with cooling systems containing just passive heat-sinks or conventional active heat-sinks constructed of a heat-sink and a fan.Research limitations/implicationsThe research carried out is the preliminary stage to asses if a compact thermal model of the investigated cooling system can be formulated.Originality/valueIn the paper, the original results of measurements and calculations of parameters of a cooling system containing a Peltier module and an active heat-sink are presented and discussed. An influence of power dissipated in the components of the cooling system on its efficiency is investigated.
目的本研究的目的是在测量选定因素对冷却系统热阻值的影响的基础上,研究包含Peltier模块的冷却系统对冷却功率设备的有用性的验证。设计/方法/方法作者建造了一个包含散热器、珀耳帖模块和风扇的冷却系统,并对珀耳帖组件和风扇在各种供应条件下的温度和热阻进行了测量和讨论。研究结果:与仅包含被动散热器或由散热器和风扇构成的传统主动散热器的冷却系统相比,在主动冷却系统中使用珀耳帖模块是否有任何好处。研究局限性/含义所进行的研究是评估是否可以制定所研究冷却系统的紧凑热模型的初步阶段。独创性/价值本文介绍并讨论了包含珀耳帖模块和有源散热器的冷却系统参数的原始测量和计算结果。研究了冷却系统各部件的功率耗散对其效率的影响。
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引用次数: 1
Improving the electromagnetic compatibility of electronic products by using response surface methodology and artificial neural network 利用响应面法和人工神经网络提高电子产品的电磁兼容性
IF 1.1 4区 工程技术 Q3 Engineering Pub Date : 2021-11-03 DOI: 10.1108/mi-06-2021-0052
Ching-Hsiang Chen, Chien-Yi Huang, Yan-Ci Huang
PurposeThe purpose of this study is to use the Taguchi Method for parametric design in the early stages of product development. electromagnetic compatibility (EMC) issues can be considered in the early stages of product design to reduce counter-measure components, product cost and labor consumption increases due to a number of design changes in the R&D cycle and to accelerate the R&D process.Design/methodology/approachThe three EMC characteristics, including radiated emission, conducted emission and fast transient impulse immunity of power, are considered response values; control factors are determined with respect to the relevant parameters for printed circuit board and mechanical design of the product and peripheral devices used in conjunction with the product are considered as noise factors. The optimal parameter set is determined by using the principal component gray relational analysis in conjunction with both response surface methodology and artificial neural network.FindingsMarket specifications and cost of components are considered to propose an optimal parameter design set with the number of grounded screw holes being 14, the size of the shell heat dissipation holes being 3 mm and the arrangement angle of shell heat dissipation holes being 45 degrees, to dispose of 390 O filters on the noise source.Originality/valueThe optimal parameter set can improve EMC effectively to accommodate the design specifications required by customers and pass test regulations.
目的本研究的目的是在产品开发的早期阶段使用田口方法进行参数化设计。电磁兼容性(EMC)问题可以在产品设计的早期阶段考虑,以减少在研发周期中由于多次设计变更而增加的对策元件、产品成本和人工消耗,加快研发进程。考虑了电源的辐射发射、传导发射和快速瞬态冲击抗扰度三个电磁兼容特性的响应值;控制因素是根据印刷电路板和产品的机械设计的相关参数确定的,与产品一起使用的外围设备被认为是噪声因素。采用响应面法和人工神经网络相结合的主成分灰色关联分析方法确定了最优参数集。考虑元器件的市场规格和成本,提出了接地螺孔数为14个,外壳散热孔尺寸为3mm,外壳散热孔布置角度为45度的最优参数设计方案,在噪声源上配置390个O型滤波器。最优的参数设置可以有效地提高EMC,以适应客户要求的设计规范,并通过测试法规。
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引用次数: 1
On the methodology of calculating volume charge density in a MIFGMOS substrate using Poisson’s equation 用泊松方程计算MIFGMOS衬底中体积电荷密度的方法
IF 1.1 4区 工程技术 Q3 Engineering Pub Date : 2021-10-14 DOI: 10.1108/mi-01-2021-0004
Francisco Javier Plascencia Jauregui, A. M. Medina Vázquez, Edwin Christian Becerra Álvarez, José Manuel Arce Zavala, Sandra Fabiola Flores Ruiz
PurposeThis study aims to present a mathematical method based on Poisson’s equation to calculate the voltage and volume charge density formed in the substrate under the floating gate area of a multiple-input floating-gate metal-oxide semiconductor metal-oxide semiconductor (MOS) transistor.Design/methodology/approachBased on this method, the authors calculate electric fields and electric potentials from the charges generated when voltages are applied to the control gates (CG). This technique allows us to consider cases when the floating gate has any trapped charge generated during the manufacturing process. Moreover, the authors introduce a mathematical function to describe the potential behavior through the substrate. From the resultant electric field, the authors compute the volume charge density at different depths.FindingsThe authors generate some three-dimensional graphics to show the volume charge density behavior, which allows us to predict regions in which the volume charge density tends to increase. This will be determined by the voltages on terminals, which reveal the relationship between CG and volume charge density and will allow us to analyze some superior-order phenomena.Originality/valueThe procedure presented here and based on coordinates has not been reported before, and it is an aid to generate a model of the device and to build simulation tools in an analog design environment.
目的本研究旨在提出一种基于泊松方程的数学方法来计算多输入浮栅金属氧化物半导体金属氧化物半导体(MOS)晶体管的浮栅区域下衬底中形成的电压和体积电荷密度。设计/方法/方法基于该方法,作者根据向控制门(CG)施加电压时产生的电荷来计算电场和电势。这种技术使我们能够考虑浮栅在制造过程中产生任何捕获电荷的情况。此外,作者还引入了一个数学函数来描述通过衬底的电势行为。根据产生的电场,作者计算了不同深度的体积电荷密度。发现作者生成了一些三维图形来显示体积电荷密度的行为,这使我们能够预测体积电荷密度趋于增加的区域。这将由端子上的电压决定,这揭示了CG和体积电荷密度之间的关系,并将使我们能够分析一些高阶现象。独创性/价值本文介绍的基于坐标的程序以前从未报道过,它有助于生成设备模型并在模拟设计环境中构建模拟工具。
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引用次数: 0
Optimization of flexible printed circuit board’s cooling with air flow and thermal effects using response surface methodology 用响应面法优化气流和热效应对柔性印刷电路板的冷却
IF 1.1 4区 工程技术 Q3 Engineering Pub Date : 2021-10-04 DOI: 10.1108/mi-06-2021-0049
Chongqing Li, M. Z. Abdullah, Ishak Abdul Aziz, C. Khor, M. S. Abdul Aziz
PurposeThis study aims to investigate the interaction of independent variables [Reynolds number (Re), thermal power and the number of ball grid array (BGA) packages] and the relation of the variables with the responses [Nusselt number ((Nu) ¯ ), deflection/FPCB’s length (d/L) and von Mises stress]. The airflow and thermal effects were considered for optimizing the Re of various numbers of BGA packages with thermal power attached on flexible printed circuit board (FPCB) for optimum cooling performance with least deflection and stress by using the response surface method (RSM).Design/methodology/approachFlow and thermal effects on FPCB with heat source generated in the BGA packages have been examined in the simulation. The interactive relationship between factors (i.e. Re, thermal power and number of BGA packages) and responses (i.e. deflection over FPCB length ratio, stress and average Nusselt number) were analysed using analysis of variance. RSM was used to optimize the Re for the different number of BGA packages attached to the FPCB.FindingsIt is important to understand the behaviour of FPCB when exposed to both flow and thermal effects simultaneously under the operating conditions. Maximum d/L and von Misses stress were significantly affected by all parametric factors whilst (Nu)¯ is significantly affected by Re and thermal power. Optimized Re for 1–3 BGA packages with maximum thermal power applied has been identified as 21,364, 23,858 and 29,367, respectively.Practical implicationsThis analysis offers a better interpretation of the parameter control in FPCB with optimized Re for the use of force convection electronic cooling. Optimal Re could be used as a reference in the thermal management aspect in designing the BGA package.Originality/valueThis research presents the parameters’ effects on the reliability and heat transfer in FPCB design. It also presents a method to optimize Re for the different number of BGA packages attached to increase the reliability in FPCB’s design.
目的研究自变量[雷诺数(Re)、热功率和球栅阵列(BGA)封装数]之间的相互作用,以及这些变量与响应[努塞尔数(Nu)¯)、挠度/FPCB长度(d/L)和von Mises应力]之间的关系。采用响应面法(RSM),考虑气流和热效应对柔性印刷电路板(FPCB)上不同数量的热功率BGA封装的Re进行优化,以获得最小挠度和应力的最佳冷却性能。设计/方法/方法在模拟中研究了BGA封装中产生的热源对FPCB的流和热效应。利用方差分析分析了各因素(Re、热功率和BGA封装数)与响应(即FPCB长度比、应力和平均努塞尔数的挠度)之间的交互关系。采用RSM法对FPCB上不同数量的BGA封装进行了优化。了解FPCB在工作条件下同时暴露于流动和热效应时的行为是很重要的。最大d/L和von miss应力受各参数影响显著,而(Nu)¯受Re和热功率影响显著。在最大热功率下,1-3 BGA封装的优化Re分别为21,364、23,858和29,367。实际意义本分析提供了一个更好的解释在FPCB参数控制与优化Re的使用力对流电子冷却。最优Re可作为BGA封装热管理设计的参考。原创性/价值本文研究了FPCB设计中参数对可靠性和传热的影响。提出了一种针对不同BGA封装数量优化Re的方法,以提高FPCB设计的可靠性。
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引用次数: 2
Influence of copper pillar bump structure on flip chip packaging during reflow soldering: a numerical approach 回流焊过程中铜柱凹凸结构对倒装芯片封装的影响:数值方法
IF 1.1 4区 工程技术 Q3 Engineering Pub Date : 2021-09-23 DOI: 10.1108/mi-05-2021-0044
M. Ishak, M. Aziz, F. Ismail, M. Z. Abdullah
PurposeThe purpose of this paper is to present the experimental and simulation studies on the influence of copper pillar bump structure on flip chip packaging during reflow soldering.Design/methodology/approachIn this work, solidification/melting modelling and volume of fluid modelling were used. Reflow soldering process of Cu pillar type FC was modelled using computational fluid dynamic software (FLUENT). The experimental results have been validated with the simulation results to prove the accuracy of the numerical method.FindingsThe findings of this study reveal that solder volume is the most important element influencing reflow soldering. The solder cap volume reduces as the Cu pillar bump diameter lowers, making the reflow process more difficult to establish a good solder union, as less solder is allowed to flow. Last but not least, the solder cap height for the reflow process must be optimized to enable proper solder joint formation.Practical implicationsThis study provides a basis and insights into the impact of copper pillar bump structure on flip chip packaging during reflow soldering that will be advancing the future design of 3D stack package. This study also provides a superior visualization and knowledge of the melting and solidification phenomenon during the reflow soldering process.Originality/valueThe computational fluid dynamics analysis of copper pillar bump structure on flip chip packaging during reflow soldering is scant. To the authors’ best knowledge, no research has been concentrated on copper pillar bump size configurations in a thorough manner. Without the in-depth study, copper pillar bump size might have the impact of copper pillar bump structure on flip chip packaging during reflow soldering. Five design of parameter of flip chip IC package model was proposed for the investigation of copper pillar bump structure on flip chip packaging during reflow soldering.
目的对回流焊过程中铜柱凹凸结构对倒装封装的影响进行了实验和仿真研究。设计/方法/方法在这项工作中,使用了凝固/熔化建模和流体体积建模。利用计算流体动力学软件FLUENT对铜柱型FC的回流焊过程进行了建模。实验结果与仿真结果进行了验证,验证了数值方法的准确性。研究结果表明,焊料体积是影响回流焊效果的最重要因素。随着铜柱凸点直径的减小,焊帽体积减小,使得回流过程更难建立良好的焊料结合,因为允许流动的焊料减少。最后但并非最不重要的是,回流工艺的焊帽高度必须优化,以使适当的焊点形成。实际意义本研究为研究回流焊过程中铜柱凹凸结构对倒装封装的影响提供了基础和见解,将推动未来3D堆叠封装的设计。本研究还提供了回流焊过程中熔化和凝固现象的可视化和知识。对倒装芯片封装中回流焊过程中铜柱凸点结构的计算流体动力学分析还很缺乏。据作者所知,目前还没有对铜柱凹凸尺寸配置进行深入的研究。如果没有深入的研究,回流焊过程中铜柱凸点的尺寸可能会对倒装芯片封装产生影响。提出了倒装IC封装模型参数的五种设计方法,以研究倒装封装回流焊过程中铜柱凹凸结构的影响。
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引用次数: 1
Computer‐aided selective production of low-resistance NiP and NiCuP layers 低阻NiP和NiCuP层的计算机辅助选择性生产
IF 1.1 4区 工程技术 Q3 Engineering Pub Date : 2021-09-22 DOI: 10.1108/mi-04-2021-0032
P. Kowalik, E. Wróbel
PurposeThis paper aims to present the possibility of computer-aided technology of chemical metallization for the production of electrodes and resistors based on Ni-P and Ni-Cu-P layers.Design/methodology/approachBased on the calculated parameters of the process, test structures were made on an alumina substrate using the selective metallization method. Dependences of the surface resistance on the metallization time were made. These dependencies take into account the comparison of the calculations with the performed experiment.FindingsThe author created a convenient and easy-to-use tool for calculating basic Ni-P and Ni-Cu-P layer parameters, namely, surface resistance and temperature coefficient of resistance (TCR) of test resistor, based on chemical metallization parameters. The values are calculated for a given level of surface resistance of Ni-P and Ni-Cu-P layer and defined required range of changes of TCR of test resistor. The calculations are possible for surface resistance values in the range of 0.4 Ohm/square ÷ 2.5 Ohm/square. As a result of the experiment, surface resistances were obtained that practically coincide with the calculations made with the use of the program created by the authors. The quality of the structures made is very good.Originality/valueTo the best of the authors’ knowledge, the paper presents a new, unpublished method of manufacturing electrodes (resistors) on silicon, Al2O3 and low temperature co-fired ceramic substrates based on the authors developed computer program.
目的介绍基于Ni-P层和Ni-Cu-P层的化学金属化计算机辅助技术生产电极和电阻器的可能性。设计/方法/方法基于计算的工艺参数,采用选择性金属化方法在氧化铝基板上制作测试结构。研究了表面电阻与金属化时间的关系。这些相关性考虑了计算与实验的比较。作者创建了一个方便易用的工具来计算基本的Ni-P和Ni-Cu-P层参数,即基于化学金属化参数的测试电阻的表面电阻和电阻温度系数(TCR)。在给定的Ni-P和Ni-Cu-P层表面电阻水平下,计算出这些值,并定义了测试电阻TCR的要求变化范围。可计算的表面电阻值范围为0.4欧姆/平方÷ 2.5欧姆/平方。实验结果表明,表面电阻的计算结果与作者编写的程序的计算结果基本一致。制作的结构质量很好。原创性/价值据作者所知,本文基于作者编写的计算机程序,提出了一种在硅、氧化铝和低温共烧陶瓷衬底上制造电极(电阻器)的新方法,该方法尚未发表。
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引用次数: 2
Preliminary dielectrophoresis study: Manipulation of protein albumin and electrical quantification by using cyclic voltammetry technique 初步的电泳研究:白蛋白的操作和循环伏安技术的电定量
IF 1.1 4区 工程技术 Q3 Engineering Pub Date : 2021-09-05 DOI: 10.1108/mi-02-2021-0026
Nur Shahira Abdul Nasir, Revathy Deivasigamani, Muhammad Khairulanwar Abdul Rahim, Siti Nur AshakirinMohd Nashruddin, A. A. Hamzah, M. R. Wee, M. R. Buyong
PurposeThe purpose of this paper is to visualize protein manipulation using dielectrophoresis (DEP) as a substantial perspective on being an effective protein analysis and biosensor method as DEP is able to be used as a means for manipulation, fractionation, pre-concentration and separation. This research aims to quantify DEP using an electrochemical technique known as cyclic voltammetry (CV), as albumin is non-visible without any fluorescent probe or dye.Design/methodology/approachThe principles of DEP were generated by an electric field on tapered DEP microelectrodes. The principle of CV was analysed using different concentrations of albumin on a screen-printed carbon electrode. Using preliminary data from both DEP and CV methods as a future prospect for the integration of both techniques to do electrical quantification of DEP forces.FindingsThe size of the albumin is known to be 0.027 µm. Engineered polystyrene particle of size 0.05 µm was selected to mimic the DEP actuation of albumin. Positive DEP of the sample engineered polystyrene particle was able to be visualized clearly at 10 MHz supplied with 20 Vpp. However, negative DEP was not able to be visualized because of the limitation of the apparatus. However, albumin was not able to be visualized under the fluorescent microscope because of its translucent properties. Thus, a method of electrical quantification known as the CV technique is used. The detection of bovine serum albumin (BSA) using the CV method is successful. As the concentration of BSA increases, the peak current obtained from the voltammogram decreases. The peak current can be an indicator of DEP response as it correlates to the adsorption of the protein onto the electrodes. The importance of the results from both CV and DEP shows that the integration of both techniques is possible.Originality/valueThe integration of both methods could give rise to a new technique with precision to be implemented into the dialyzers used in renal haemodialysis treatment for manipulation and sensing of protein albumin.
目的:本文的目的是可视化的蛋白质操作使用电介质电泳(DEP)作为一个有效的蛋白质分析和生物传感器方法的实质性观点,因为DEP能够用作操作,分馏,预浓缩和分离的手段。这项研究的目的是使用一种称为循环伏安法(CV)的电化学技术来量化DEP,因为白蛋白在没有任何荧光探针或染料的情况下是不可见的。DEP的原理是通过在锥形DEP微电极上施加电场产生的。在丝网印刷的碳电极上使用不同浓度的白蛋白,分析了CV的原理。利用DEP和CV方法的初步数据作为两种技术集成的未来前景,以进行DEP力的电量化。结果白蛋白的大小为0.027µm。选择尺寸为0.05µm的工程聚苯乙烯颗粒来模拟白蛋白的DEP驱动。样品工程聚苯乙烯颗粒的正DEP能够在10 MHz和20 Vpp下清晰地显示。然而,由于仪器的限制,负DEP无法显示。然而,由于白蛋白的半透明性质,在荧光显微镜下无法看到。因此,使用了一种称为CV技术的电量化方法。用CV法检测牛血清白蛋白(BSA)是成功的。随着BSA浓度的增加,从伏安图中得到的峰值电流减小。峰值电流可以作为DEP响应的一个指标,因为它与蛋白质在电极上的吸附有关。CV和DEP结果的重要性表明,两种技术的整合是可能的。两种方法的整合可以产生一种新的精确技术,用于肾血液透析治疗的透析器中,用于蛋白质白蛋白的操作和传感。
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引用次数: 2
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Microelectronics International
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