Chemosphere最新文献_第8页

Polystyrene nanoplastic exposure increases susceptibility of Pseudomonas aeruginosa infection in Caenorhabditis elegans model of host-pathogen interaction in p38-MAP Kinase dependent manner 在p38-MAP激酶依赖的秀丽隐杆线虫宿主-病原体相互作用模型中，聚苯乙烯纳米塑料暴露增加铜绿假单胞菌感染的易感性。

IF 8.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Chemosphere

Pub Date : 2025-11-20 DOI: 10.1016/j.chemosphere.2025.144774

Acharya Balkrishna , Saurabh Bhatti , Sandeep Kumar , Manisha Kabdwal , Kunal Bhattacharya , Savita Lochab , Rishabh Dev , Anurag Varshney

Nanoplastics have been shown to be potential environmental hazardous material, however not much is known on their role in modulating infection susceptibility and host-pathogen relationship. Present study investigated the role of polystyrene nanoparticles (PSNP) on infectivity of pathogenic Pseudomonas aeruginosa in Caenorhabditis elegans model. Physiochemical characterization of PSNP was done by Dynamic Light Scattering (DLS) and Fourier Transform Infrared Spectroscopy (FTIR). The internalization in C. elegans was determined by FTIR. Effect of PSNP in worms infected with P. aeruginosa was assessed through various toxicological parameters including survival, bacterial load, growth, behavior, and reproduction. PSNP (∼100 nm in size) exposure to P. aeruginosa-infected C. elegans did not alter their survivability, but affected the transition from larval stage to adult worms. PSNP exposure also affected the growth, progeny and locomotory behavior in P. aeruginosa-infected worms. FTIR analysis readily confirmed PSNP internalization in C. elegans. Interestingly, PSNP-exposure to mutant C. elegans, with compromised innate immunity due to p38-MAP Kinase deletion, resulted in enhanced susceptibility to P. aeruginosa infection, as evident from more severe defects in locomotory and reproductive behavior compared to P. aeruginosa alone infected worms. Overall, these results revealed that PSNP exposure enhanced susceptibility to P. aeruginosa infection by possibly compromising the immune response. This study emphasized the importance of understanding the hazardous effects of nanoplastic exposure on host-pathogen relation in humans.

纳米塑料已被证明是潜在的环境有害物质，但其在调节感染易感性和宿主-病原体关系中的作用尚不清楚。本文研究了聚苯乙烯纳米颗粒（PSNP）对秀丽隐杆线虫致病性铜绿假单胞菌感染的影响。采用动态光散射（DLS）和傅里叶变换红外光谱（FTIR）对PSNP进行了理化表征。FTIR法测定线虫的内化程度。PSNP对感染铜绿假单胞菌的蠕虫的影响通过各种毒理学参数进行评估，包括生存、细菌负荷、生长、行为和繁殖。暴露于铜绿假单胞菌感染的秀丽隐杆线虫的PSNP（约100 nm大小）不改变它们的生存能力，但影响了从幼虫阶段到成虫阶段的过渡。PSNP暴露也会影响铜绿假单胞菌感染蠕虫的生长、后代和运动行为。FTIR分析很容易证实了秀丽隐杆线虫中PSNP的内化。有趣的是，psnp暴露于突变的秀丽隐杆线虫，由于p38-MAP激酶缺失导致先天免疫受损，导致对铜绿假单胞菌感染的易感性增强，这一点从与单独感染铜绿假单胞菌的蠕虫相比，更严重的运动和生殖行为缺陷可见一斑。总的来说，这些结果表明PSNP暴露可能通过损害免疫反应而增加对铜绿假单胞菌感染的易感性。这项研究强调了了解纳米塑料暴露对人类宿主-病原体关系的有害影响的重要性。

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引用次数: 0

Rethinking soil contamination assessment for urban brownfield management: from total hazard to bioavailability 城市棕地管理中土壤污染评价的再思考：从总危害到生物可利用性。

IF 8.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Chemosphere

Pub Date : 2025-11-20 DOI: 10.1016/j.chemosphere.2025.144769

Rachel Seillier , Frédéric Paran , Benjamin Pauget , Cédric Challaye , Steve Peuble , Frédéric Gallice , Jérémie Riquier , Baptiste Bouillot , Olivier Faure

Hazard identification at contaminated sites usually rely on total concentrations of potentially toxic elements (PTE), but it is largely admitted that only the bioavailable fraction can trigger adverse effects. Assessing total PTE amounts is thus a worst-case scenario, that overestimates environmental risks and often leads to costly remediation actions. This study presents a practical and cost-effective methodology for assessing soil contamination by PTE, taking into account their bioavailability. To address this issue, we performed a three-tiered hazard identification considering: (1) total hazard (total PTE content), (2) environmentally available hazard (CaCl₂ leachable fraction), and (3) environmentally bioavailable hazard (accumulation in plant leaves). Additionally, an Integrated Hazard Excess (IHE) index was computed, for aggregating raw contaminant levels into a single integrated hazard value. The methodology was applied to a former industrial site in Saint-Étienne, France. Soil and plant samples were analyzed for As, Cd, Cr, Cu, Ni, Pb, and Zn. Mapping of IHE values provided a clear and holistic view of contamination across the site. Results showed that while total PTE levels were very high, the mobile and bioavailable fractions were overall within the range of local background observed in urban parks used for recreational activities. This indicated that actual environmental risk was significantly lower than total concentrations suggested. Thus, distinguishing total, available, and phytoavailable PTE amounts provided a more accurate and ecologically meaningful assessment of soil hazard. This approach could help decision-making and support the adoption of less disruptive and more sustainable remediation strategies, avoiding unnecessary excavation and landfill operations.

污染场所的危害识别通常依赖于潜在有毒元素（PTE）的总浓度，但人们普遍承认，只有生物可利用部分才能引发不良影响。因此，评估PTE总量是最坏的情况，它高估了环境风险，往往导致代价高昂的补救行动。本研究提出了一种实用且具有成本效益的方法来评估PTE对土壤的污染，并考虑了它们的生物有效性。为了解决这个问题，我们进行了三层危害识别，考虑：(1)总危害（总PTE含量），(2)环境可利用危害（CaCl2可浸出分数），(3)环境生物可利用危害（植物叶片积累）。此外，还计算了综合危害过量指数（IHE），将原始污染物水平汇总为单个综合危害值。该方法应用于法国圣-Étienne的一个前工业区。分析了土壤和植物样品中的砷、镉、铬、铜、镍、铅和锌。IHE值的映射提供了整个站点污染的清晰和整体视图。结果表明，虽然城市公园的PTE总量非常高，但其流动组分和生物可利用组分总体上在当地背景范围内。这表明实际环境风险显著低于总浓度。因此，区分总的、有效的和植物有效的PTE量提供了更准确和有生态意义的土壤危害评估。这种方法有助于决策，并支持采用破坏性较小、更可持续的补救策略，避免不必要的挖掘和填埋作业。

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引用次数: 0

Determination of environmental estrogens (2018–2024): greener extraction and integrated workflows for water, wastewater and solid matrices aligned with regulatory monitoring needs 环境雌激素的测定（2018-2024）：更环保的提取和符合监管监测需求的水、废水和固体基质的集成工作流程。

IF 8.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Chemosphere

Pub Date : 2025-11-19 DOI: 10.1016/j.chemosphere.2025.144761

Marianna Piosik , Joanna Antos , Joanna Zembrzuska , Dobrochna Ginter-Kramarczyk , Izabela Kruszelnicka

Environmental estrogens are high-potency endocrine-disrupting contaminants detected across aquatic and terrestrial compartments. This review synthesizes advances from 2018 to 2024 in sample-preparation and extraction workflows for surface waters, wastewaters, and solid matrices, emphasizing approaches that enable trace-level quantification and are transferable to international monitoring. Solid-phase extraction remains the workhorse for aqueous samples, while greener and miniaturized formats including magnetic solid-phase extraction, dispersive liquid–liquid microextraction, thin-film microextraction, and deep eutectic solvent extractions reduce solvent footprints and processing time without sacrificing sensitivity. We evaluate matrix-driven performance with a focus on conjugate stability, potential deconjugation artefacts during sample storage and preparation, and their implications for reporting free versus total estrogens. Because environmental quality standards for key estrogens, notably EE2, approach low-picogram-per-liter levels, we benchmark reported detection limits against EU monitoring requirements (e.g., Watch List/EQS) and clarify IDL versus MDL definitions and reporting practices to improve comparability across studies. Collectively, these developments advance sensitive, scalable and more sustainable monitoring of environmental estrogens and support exposure and risk assessment across bio-, hydro- and lithospheric compartments.

环境雌激素是通过水生和陆地隔间检测到的高效内分泌干扰污染物。本文综述了2018年至2024年在地表水、废水和固体基质的样品制备和提取工作流程方面的进展，强调了能够实现痕量水平量化并可转移到国际监测的方法。固相萃取仍然是水相样品的主要方法，而更环保和小型化的形式包括磁性固相萃取、分散液液微萃取、薄膜微萃取和深共晶溶剂萃取，在不牺牲灵敏度的情况下减少了溶剂占用和处理时间。我们评估了基质驱动的性能，重点关注偶联稳定性，样品储存和制备过程中潜在的解偶联伪影，以及它们对报告游离雌激素和总雌激素的影响。由于主要雌激素的环境质量标准，特别是EE2，接近每升低皮克水平，我们根据欧盟监测要求（例如观察名单/EQS）对报告的检测限进行基准测试，并澄清IDL与MDL的定义和报告实践，以提高研究之间的可比性。总的来说，这些发展促进了对环境雌激素的敏感、可扩展和更可持续的监测，并支持跨生物、水文和岩石圈隔间的暴露和风险评估。

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引用次数: 0

Identifying soil drivers of rice productivity under fly ash and organic amendments using explainable machine learning 利用可解释的机器学习识别粉煤灰和有机修正下水稻生产力的土壤驱动因素。

IF 8.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Chemosphere

Pub Date : 2025-11-19 DOI: 10.1016/j.chemosphere.2025.144777

Soumyajeet Pradhan , Prasanna Kumar Samant , Rabindra Kumar Nayak , Meenakhi Prusty , Tushar Ranjan Mohanty , Abhiram Dash , Kumbha Karna Rout , Anshuman Nayak , Saheed Garnaik

Declining soil quality and nutrient imbalances constrain rice productivity in tropical acidic soils. The agricultural reuse of fly ash (FA), an industrial by-product, offers potential as a soil amendment when combined with organic inputs, yet mechanistic understanding of its effects on soil–yield relationships remain limited. Traditional statistical methods often fail to decode non-linear soil-yield relationships, necessitating advanced machine learning (ML) approaches. A field experiment evaluated the integrated effect of FA (10–40 t ha⁻¹), FYM (5 t ha⁻¹), and NPK effects on soil physio-chemical and biological properties and identified key soil predictors driving rice productivity using explainable machine learning. The FA40 + FYM + NPK treatment achieved the highest grain yield (54.0 q ha⁻¹), outperforming NPK alone by 38.5 %. This treatment improved soil porosity (45.5 %), water-holding capacity (37.8 %), available nitrogen (212.9 kg ha⁻¹), available phosphorus (19.6 kg ha⁻¹), and microbial enzyme activities, including urease (22.9 μg NH₄⁺-N g⁻¹ hr⁻¹) and β-glucosidase (15.1 μg pNP g⁻¹ hr⁻¹). Machine learning interpretation revealed β-glucosidase, organic carbon, urease, available phosphorus, and clay content as dominant predictors of yield variation. Conditional partial dependence plots revealed synergistic interactions between β-glucosidase and organic carbon, and between urease and available phosphorus, indicating that carbon turnover and nutrient mineralization jointly regulated yield response. These findings demonstrate that the combination of FA (20–40 t ha⁻¹) with FYM and NPK can improve soil functionality and sustain rice productivity. Explainable modelling provides mechanistic insight for advancing soil health assessment and fertilizer strategies in acidic agroecosystems.

土壤质量下降和养分失衡制约了热带酸性土壤的水稻生产力。粉煤灰（FA）是一种工业副产品，当与有机投入物结合使用时，其农业再利用具有土壤改进剂的潜力，但对其对土壤产量关系影响的机制理解仍然有限。传统的统计方法往往无法解码非线性土壤产量关系，需要先进的机器学习（ML）方法。田间试验评估了FA （10-40 t ha-1）、FYM （5 t ha-1）和NPK对土壤理化和生物特性的综合影响，并利用可解释的机器学习确定了驱动水稻生产力的关键土壤预测因子。FA40 + FYM + NPK处理籽粒产量最高（54.0 q ha-1），比单施NPK处理高出38.5%。该处理改善了土壤孔隙度（45.5%）、持水量（37.8%）、有效氮（212.9 kg ha-1）、有效磷（19.6 kg ha-1）和微生物酶活性，包括脲酶（22.9 μg NH4+-N g-1 hr-1）和β-葡萄糖苷酶（15.1 μg pNP g-1 hr-1）。机器学习解释显示β-葡萄糖苷酶、有机碳、脲酶、有效磷和粘土含量是产量变化的主要预测因子。条件部分依赖图显示，β-葡萄糖苷酶与有机碳、脲酶与速效磷之间存在协同作用，表明碳周转和养分矿化共同调控产量响应。综上所述，FA （20 ~ 40 t hm -1）与FYM和NPK配施可改善土壤功能，维持水稻生产力。可解释的模型为酸性农业生态系统中推进土壤健康评估和施肥策略提供了机制见解。

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引用次数: 0

Using integrated biomarkers to assess ecological risk from multi-metal and metalloid soil contamination 综合生物标志物评价多金属和类金属土壤污染生态风险

IF 8.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Chemosphere

Pub Date : 2025-11-19 DOI: 10.1016/j.chemosphere.2025.144770

Marion Sarah Deville-Cavellin , Floriane Guillevic , Christelle Gonindard-Melodelima , Sylvain Campillo-Cressot , Frédéric Laporte , Cindy Arnoldi , Magali Rossi , Arnaud Foulquier , Muriel Raveton

Soil contamination with trace metals and metalloids (TMM) can negatively impact biota in ways not captured by chemical analyses alone. This study evaluates the effectiveness of two integrated biological indices (Total Enzyme activity Index – TEI, Integrated Biomarker Response – IBR) in detecting toxicity effects on biota in soils subjected to long-term TMM contamination. An ex situ biotest using soils from Peisey-Nancroix (France), a former Pb–Ag mining site, was conducted with the sensitive plant Arabidopsis thaliana to identify relevant biomarkers and develop key biological indices. The results demonstrate clear TMM toxicity, reflected in decreased microbial functioning (evidenced by reduced TEI) and impaired plant growth (shown by elevated IBR). These indices were then assessed in situ at the same site. Unexpectedly, TEI—despite its status as an early indicator of soil degradation—showed no relationship with long-term TMM toxicity or Pb-mobility, whereas IBR—derived from endogenous plant biomarkers—strongly correlated with TMM contamination and bioavailable Pb levels, particularly in the sensitive species Geranium sylvaticum. These results indicate that IBR is a reliable proxy for ecological risk and TMM bioavailability in chronically contaminated soils.

土壤中微量金属和类金属（TMM）的污染会以化学分析无法捕捉到的方式对生物群产生负面影响。本研究评估了两种综合生物指标（总酶活性指数- TEI，综合生物标志物反应- IBR）在检测长期TMM污染土壤中生物群毒性效应中的有效性。利用法国peiseey - nancroix（原铅银矿区）土壤对敏感植物拟南芥（Arabidopsis thaliana）进行了迁地生物试验，以鉴定相关生物标志物并建立关键生物学指标。结果显示了明显的TMM毒性，反映在微生物功能降低（TEI降低）和植物生长受损（IBR升高）。然后在同一地点就地评估这些指标。出乎意料的是，尽管tei是土壤退化的早期指标，但它与TMM的长期毒性或Pb迁移性没有关系，而ibr——来自内源植物生物标志物——与TMM污染和生物可利用Pb水平密切相关，尤其是在敏感物种天竺葵中。这些结果表明，IBR是长期污染土壤生态风险和TMM生物有效性的可靠指标。

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引用次数: 0

Corrigendum to “Microplastic assessment in remote and high mountain lakes of Gilgit Baltistan, Pakistan” [Chemosphere 365 (2024) 143283] “吉尔吉特巴尔蒂斯坦偏远和高山湖泊的微塑料评估，巴基斯坦”的勘误表[Chemosphere 365(2024) 143283]。

IF 8.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Chemosphere

Pub Date : 2025-11-19 DOI: 10.1016/j.chemosphere.2025.144740

Maryem Mehboob , Rachid Dris , Bruno Tassin , Johnny Gasperi , Muhammad Usman Khan , Riffat N. Malik

引用次数: 0

Tolerance of the apple snail Pomacea canaliculata to individual and combined exposures of the pyrethroid cypermethrin and the tetramic acid spirotetramat 苹果螺Pomacea canaliculata对拟除虫菊酯、氯氰菊酯和四乙酸螺虫酯单独和联合暴露的耐受性。

IF 8.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Chemosphere

Pub Date : 2025-11-18 DOI: 10.1016/j.chemosphere.2025.144766

Manuela Vattiato , Sonia M. Landro , Fernando G. Spaccesi , Florencia Arrighetti , Sabrina M.L. Lavarías

This study evaluated the sensitivity of the apple snail Pomacea canaliculata to the insecticides cypermethrin (CYP), a type II pyrethroid, and spirotetramat (STM), a new-generation tetramic acid. Initially, the LC₅₀ values were determined for adult male and female snails, revealing a high tolerance to both compounds, with values of 65.6 mg/L for CYP and 12.8 mg/L for STM. Subsequently, female snails were exposed to sublethal concentrations of both pesticides individually and in combination for ten days. Several biomarkers, including biochemical parameters and histopathological changes, were studied in the digestive gland. The two-factor ANOVA analysis showed that of all the biochemical parameters evaluated, only total protein, uric acid, glutathione S-transferase, and lactate dehydrogenase activity were affected by individual CYP treatment (0.1 mg/L) compared to the control. Additionally, lactate dehydrogenase activity was affected by individual and combined STM treatments, showing a hormesis pattern. Triacylglycerol content showed no differences between the control and the different treatments. However, the 0.1 mg/L CYP +0.1 mg/L STM treatment resulted in the highest triacylglycerol accumulation. Semi-quantitative analysis of the histopathological index (HI) revealed that the exposure of both CYP and STM led to a significantly higher HI than in controls. However, no dose-dependent pattern was observed, suggesting a high sensitivity of the digestive gland. The integrated biomarker index (IBRv2) analysis showed that CYP caused greater damage than STM. The combined treatment exhibited an antagonistic interaction between CYP and STM. In conclusion, this study demonstrates the high tolerance of P. canaliculata to pesticides from different chemical groups. This remarkable adaptability may explain its success in adverse environmental conditions, establishing it as one of the world's most damaging invasive species. The study also reinforces the value of histopathological changes as the most appropriate and sensitive biomarker for assessing pesticide contamination in this organism.

本研究评价了苹果螺Pomacea canaliculata对II型拟除虫菊酯类杀虫剂氯氰菊酯（CYP）和新一代四羧酸类杀虫剂螺虫菊酯（STM）的敏感性。最初，测定了成年雄性和雌性蜗牛的LC50值，显示出对这两种化合物的高耐受性，CYP的值为65.6 mg/L， STM的值为12.8 mg/L。随后，雌性蜗牛分别暴露于亚致死浓度的杀虫剂和组合杀虫剂中10天。研究了几种生物标志物，包括生化参数和组织病理学变化。双因素方差分析显示，与对照组相比，单个CYP处理（0.1 mg/L）仅影响总蛋白、尿酸、谷胱甘肽s -转移酶和乳酸脱氢酶活性。此外，乳酸脱氢酶活性受到单独和联合STM处理的影响，表现出激效模式。三酰甘油含量在对照和不同处理间无显著差异。然而，0.1 mg/L CYP +0.1 mg/L STM处理的甘油三酯积累量最高。半定量分析的组织病理学指数（HI）显示，暴露于CYP和STM导致HI显著高于对照组。然而，没有观察到剂量依赖性模式，表明消化腺具有高敏感性。综合生物标志物指数（IBRv2）分析显示，CYP造成的损伤大于STM。联合处理显示出CYP与STM之间的拮抗相互作用。综上所述，本研究证明了小管藤对不同化学类群的农药具有较高的耐受性。这种非凡的适应能力可以解释它在恶劣环境条件下的成功，使其成为世界上最具破坏性的入侵物种之一。该研究还强调了组织病理学变化作为评估该生物体内农药污染最合适和最敏感的生物标志物的价值。

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引用次数: 0

Fluorene-induced antioxidant disruption, neurophysiological impairment, and DNA damage in freshwater snail (Bellamya bengalensis) and fish (Anabas testudineus): Evidence from in vivo and in silico studies 氟诱导的淡水蜗牛（Bellamya bengalensis）和鱼类（Anabas testudineus）的抗氧化破坏、神经生理损伤和DNA损伤：来自体内和计算机研究的证据。

IF 8.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Chemosphere

Pub Date : 2025-11-18 DOI: 10.1016/j.chemosphere.2025.144776

Azubuike Chukwuka , Pramita Sharma , Soumendranath Chatterjee , Nimai Chandra Saha

Fluorene (FL), a low-molecular-weight polycyclic aromatic hydrocarbon (PAH), is increasingly detected in freshwater environments, yet its mechanistic toxicity remains poorly characterized. This study evaluated FL-induced toxicity in Bellamya bengalensis (BB) and Anabas testudineus (AT) under acute (96hrs) and chronic exposures (28 and 45 days respectively). Acute assays revealed concentration-dependent mortality, with 96-h LC₅₀ values of 3.074 mg/L for BB and 1.991 mg/L for AT, indicating higher fish sensitivity. Behavioral impairments—reduced crawling and mucus secretion in BB, and erratic swimming, equilibrium loss, and opercular changes in AT—signaled early neurophysiological disruption. Chronic exposure (10 % and 20 % LC₅₀) caused significant oxidative stress, tissue damage, and DNA fragmentation, corroborated by elevated tail length and tail moment in the comet assay. Molecular docking provided mechanistic support, revealing strong fluorene affinity for catalase and DNA polymerase in BB (−6.8 and −6.1 kcal/mol) versus DNA topoisomerase in AT (−6.2 kcal/mol). These interactions, dominated by π–π stacking and hydrophobic contacts, suggest catalytic inhibition and structural destabilization of antioxidant and genomic enzymes. Consequently, oxidative stress emerged as the primary toxicity pathway in BB, while replication stress and genotoxicity dominated in AT. Histopathological lesions—epithelial atrophy in BB and hepatocellular hypertrophy in AT—further reflected divergent metabolic responses. Collectively, the cross-species evidence highlights that even a structurally simple PAH such as fluorene exerts complex, multi-level effects through conserved molecular targets. These findings underscore species-specific vulnerabilities and demonstrate the ecological and regulatory importance of incorporating in silico–in vivo integration in aquatic PAH risk assessment.

芴（FL）是一种低分子量的多环芳烃（PAH），在淡水环境中被越来越多地检测到，但其机制毒性的研究仍然很少。本研究评估了fl在急性暴露（96小时）和慢性暴露（28天和45天）下对孟加拉贝拉米亚鼠（BB）和testudineus （AT）的毒性。急性试验显示出浓度依赖性死亡率，BB的96小时LC50值为3.074 mg/L， AT的LC50值为1.991 mg/L，表明鱼的敏感性更高。行为障碍- BB中爬行和粘液分泌减少，at中游泳不稳定，平衡丧失和眼角膜改变标志着早期神经生理障碍。长期暴露（10%和20% LC50）引起显著的氧化应激、组织损伤和DNA断裂，彗星试验中尾巴长度和尾巴力矩的增加证实了这一点。分子对接提供了机制支持，揭示了BB中过氧化氢酶和DNA聚合酶（-6.8和-6.1 kcal/mol）与AT中的DNA拓扑异构酶（-6.2 kcal/mol）具有很强的芴亲和力。这些相互作用以π-π堆积和疏水接触为主，表明了抗氧化酶和基因组酶的催化抑制和结构不稳定。因此，氧化应激是BB的主要毒性途径，而复制应激和遗传毒性在AT中占主导地位。组织病理学病变——BB的上皮萎缩和at的肝细胞肥大进一步反映了不同的代谢反应。总的来说，跨物种的证据表明，即使是结构简单的多环芳烃，如芴，也会通过保守的分子靶点产生复杂的、多层次的影响。这些发现强调了物种特异性脆弱性，并证明了将硅-体内整合纳入水生多环芳烃风险评估的生态和调控重要性。

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引用次数: 0

Identification and quantification of per- and polyfluoroalkyl substances in wastewater from Taiwan using targeted and non-targeted approaches 使用目标和非目标方法鉴定和量化台湾废水中的全氟烷基和多氟烷基物质。

IF 8.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Chemosphere

Pub Date : 2025-11-18 DOI: 10.1016/j.chemosphere.2025.144762

Jen-Yi Hsu , Chih-Wei Chang , Yu-Wen Lin , Yuan-Chih Chen , Pao-Chi Liao

This study assessed per- and polyfluoroalkyl substances (PFAS) in municipal wastewater influents in Taiwan using a high-resolution mass spectrometry (HRMS) workflow combining targeted and non-targeted analyses. Targeted analysis detected seven PFAS above quantification, with perfluorooctane sulfonate (PFOS, 5.33–8.37 ng/L) at the highest levels, followed by PFHxS (1.31–2.88 ng/L), PFBS (0.88–1.26 ng/L), and 6:2 fluorotelomer sulfonate (up to 3.40 ng/L). Short-chain acids such as PFBA and PFPeA were mostly below quantification. Non-targeted screening expanded the PFAS inventory to 45 compounds, including ultra-short-chain PFAS (e.g., trifluoroacetic acid, trifluoromethanesulfonic acid) and multiple precursors. The diversity of detected PFAS underscores the importance of integrating non-targeted strategies into wastewater monitoring. These findings provide the first nationwide PFAS profile for Taiwanese municipal wastewater and establish a baseline for future environmental surveillance and regulatory development.

本研究利用高分辨率质谱（HRMS）结合目标分析和非目标分析的工作流程，评估了台湾城市污水中的全氟烷基和多氟烷基物质（PFAS）。目标分析检测出定量以上的7种PFAS，其中全氟辛烷磺酸（PFOS, 5.33-8.37 ng/L）含量最高，其次是PFHxS （1.31-2.88 ng/L）、PFBS （0.88-1.26 ng/L）和6:2氟端聚物磺酸（高达3.40 ng/L）。短链酸如PFBA和PFPeA大多无法定量。非靶向筛选将PFAS清单扩大到45种化合物，包括超短链PFAS（如三氟乙酸、三氟甲烷磺酸）和多种前体。检测到的PFAS的多样性强调了将非目标战略纳入废水监测的重要性。这些发现提供了台湾城市污水的第一个全国性PFAS概况，并为未来的环境监测和监管发展奠定了基础。

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引用次数: 0

The influence of BPA on isometric contraction, cholinergic activity, and antioxidant status in the crop gizzard of Lumbricus terrestris 双酚a对蚓囊等长收缩、胆碱能活性和抗氧化状态的影响。

IF 8.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Chemosphere

Pub Date : 2025-11-17 DOI: 10.1016/j.chemosphere.2025.144771

Zofia Goc, Zuzanna Mucha, Edyta Kapusta, Grzegorz Formicki

The billions of tons of bisphenol A (BPA)-containing waste constitutes a serious concern. BPA leaching from waste through water is a major source of soil contamination. BPA enters the body primarily through ingestion and affects cellular redox balance and cholinergic neuronal activity. The aim of this study was to assess gastrointestinal motility, acetylcholinesterase (AChE) activity, antioxidant enzyme activity (SOD, CAT, and GPx), and the GSH, MDA, and choline (Ch) concentrations in the digestive systems of Lumbricus terrestris earthworms reared for 7 d in soil containing various BPA concentrations. After the 7-d exposure period, crop gizzards were collected from the digestive tracts for analysis. BPA exposure caused an increase in AChE activity, except in animals exposed to the highest BPA concentration. Changes in cholinergic activity were confirmed via Ch concentration measurements. We observed that BPA exerted a relaxant effect on crop gizzard muscles, with statistically significant decreases in the frequency and maximum force of spontaneous isometric contractions (ICs). The IC parameters induced by acetylcholine chloride (AChC) were lower in animals reared in BPA-contaminated soil than in those of the control group. Statistically significant reductions in the mean and minimum IC forces were observed. SOD activity and GPx activity increased in response to low and medium BPA concentrations but decreased in animals exposed to the highest BPA concentration. A similar pattern was observed for the GSH concentration. CAT activity notably decreased in a BPA concentration-dependent manner. Additionally, we observed a statistically significant and BPA concentration-dependent increase in the MDA concentration.

数十亿吨含双酚A （BPA）的废物构成了一个严重的问题。通过水从废物中浸出BPA是土壤污染的主要来源。BPA主要通过摄入进入人体，影响细胞氧化还原平衡和胆碱能神经元活动。本研究的目的是评估在不同BPA浓度土壤中饲养7 d的陆生蚓的胃肠蠕动、乙酰胆碱酯酶（AChE）活性、抗氧化酶（SOD、CAT和GPx）活性以及消化系统中GSH、MDA和胆碱（Ch）浓度。暴露7 d后，从消化道采集作物砂囊进行分析。除了暴露在BPA浓度最高的动物外，BPA暴露导致乙酰胆碱酯酶活性增加。通过测定Ch浓度证实胆碱能活性的变化。我们观察到BPA对作物砂囊肌肉有松弛作用，自发等长收缩（ICs）的频率和最大力有统计学意义上的显著降低。在双酚a污染土壤中饲养的动物，乙酰胆碱氯（AChC）诱导的IC参数低于对照组。观察到平均和最小IC力在统计上显着降低。低、中BPA浓度下，SOD活性和GPx活性升高，高BPA浓度下，SOD活性和GPx活性降低。谷胱甘肽浓度也有类似的变化。CAT活性以BPA浓度依赖性显著降低。此外，我们观察到具有统计学意义且BPA浓度依赖性的MDA浓度增加。

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