首页 > 最新文献

中国当代儿科杂志最新文献

英文 中文
[Berberine ameliorates coronary artery endothelial cell injury in Kawasaki disease through complement and coagulation cascades]. [小檗碱通过补体和凝血级联改善川崎病冠状动脉内皮细胞损伤]。
Q3 Medicine Pub Date : 2025-01-15 DOI: 10.7499/j.issn.1008-8830.2406075
Jin-Wen Liao, Xin Guo, Bo Liang, Xu-Xia Li, Ming-Guo Xu

Objectives: To explore the role of berberine (BBR) in ameliorating coronary endothelial cell injury in Kawasaki disease (KD) by regulating the complement and coagulation cascade.

Methods: Human coronary artery endothelial cells (HCAEC) were divided into a healthy control group, a KD group, and a BBR treatment group (n=3 for each group). The healthy control group and KD group were supplemented with 15% serum from healthy children and KD patients, respectively, while the BBR treatment group received 15% serum from KD patients followed by the addition of 20 mmol/L BBR. Differential protein expression was analyzed and identified using isobaric tags for relative and absolute quantitation technology and liquid chromatography-tandem mass spectrometry, followed by GO functional enrichment analysis and KEGG signaling pathway enrichment analysis of the differential proteins. Western blot was used to detect differential protein expression.

Results: A total of 518 differential proteins were identified between the KD group and the healthy control group (300 upregulated proteins and 218 downregulated proteins). A total of 422 differential proteins were identified between the BBR treatment group and the KD group (221 upregulated proteins and 201 downregulated proteins). Bioinformatics analysis showed that compared to the healthy control group, the differential proteins in the KD group were enriched in the complement and coagulation cascade and ribosome biogenesis in eukaryotes. Compared to the KD group, the differential proteins in the BBR treatment group were also enriched in the complement and coagulation cascade and ribosome biogenesis in eukaryotes. Western blot results indicated that compared to the healthy control group, the expression of complement C1q subcomponent subunit C (C1QC), kininogen-1 (KNG1), complement C1s subcomponent (C1S), and C4b-binding protein alpha chain (C4BPA) was increased in the KD group (P<0.05). Compared to the KD group, the expression of KNG1, C1S, C1QC, and C4BPA was decreased in the BBR treatment group (P<0.05).

Conclusions: The complement and coagulation cascade may be involved in the regulation of BBR treatment for coronary injury in KD, and C1QC, KNG1, C1S, and C4BPA may serve as biomarkers for this treatment.

目的:探讨小檗碱(BBR)通过调节补体和凝血级联改善川崎病(KD)冠状动脉内皮细胞损伤的作用。方法:将人冠状动脉内皮细胞(HCAEC)分为健康对照组、KD组和BBR治疗组(每组n=3)。健康对照组和KD组分别添加15%的健康儿童血清和KD患者血清,BBR治疗组添加15%的KD患者血清,然后添加20 mmol/L BBR。采用等压标签相对定量和绝对定量技术、液相色谱-串联质谱技术对差异蛋白表达进行分析鉴定,并对差异蛋白进行GO功能富集分析和KEGG信号通路富集分析。Western blot检测差异蛋白表达。结果:KD组与健康对照组之间共鉴定出518个差异蛋白(300个上调蛋白,218个下调蛋白)。在BBR处理组和KD组之间共鉴定出422个差异蛋白(221个上调蛋白和201个下调蛋白)。生物信息学分析表明,与健康对照组相比,KD组的差异蛋白在真核生物的补体、凝血级联和核糖体生物发生中富集。与KD组相比,BBR处理组的差异蛋白在真核生物的补体、凝血级联和核糖体生物发生中也富集。Western blot结果显示,与健康对照组相比,KD组补体C1q亚组分亚单位C (C1QC)、kinino原-1 (KNG1)、补体C1s亚组分(C1s)和c4b结合蛋白α链(C4BPA)的表达增加(ppp)。结论:补体和凝血级联可能参与了KD患者BBR治疗冠状动脉损伤的调控,C1QC、KNG1、C1s和C4BPA可能是该治疗的生物标志物。
{"title":"[Berberine ameliorates coronary artery endothelial cell injury in Kawasaki disease through complement and coagulation cascades].","authors":"Jin-Wen Liao, Xin Guo, Bo Liang, Xu-Xia Li, Ming-Guo Xu","doi":"10.7499/j.issn.1008-8830.2406075","DOIUrl":"10.7499/j.issn.1008-8830.2406075","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the role of berberine (BBR) in ameliorating coronary endothelial cell injury in Kawasaki disease (KD) by regulating the complement and coagulation cascade.</p><p><strong>Methods: </strong>Human coronary artery endothelial cells (HCAEC) were divided into a healthy control group, a KD group, and a BBR treatment group (<i>n</i>=3 for each group). The healthy control group and KD group were supplemented with 15% serum from healthy children and KD patients, respectively, while the BBR treatment group received 15% serum from KD patients followed by the addition of 20 mmol/L BBR. Differential protein expression was analyzed and identified using isobaric tags for relative and absolute quantitation technology and liquid chromatography-tandem mass spectrometry, followed by GO functional enrichment analysis and KEGG signaling pathway enrichment analysis of the differential proteins. Western blot was used to detect differential protein expression.</p><p><strong>Results: </strong>A total of 518 differential proteins were identified between the KD group and the healthy control group (300 upregulated proteins and 218 downregulated proteins). A total of 422 differential proteins were identified between the BBR treatment group and the KD group (221 upregulated proteins and 201 downregulated proteins). Bioinformatics analysis showed that compared to the healthy control group, the differential proteins in the KD group were enriched in the complement and coagulation cascade and ribosome biogenesis in eukaryotes. Compared to the KD group, the differential proteins in the BBR treatment group were also enriched in the complement and coagulation cascade and ribosome biogenesis in eukaryotes. Western blot results indicated that compared to the healthy control group, the expression of complement C1q subcomponent subunit C (C1QC), kininogen-1 (KNG1), complement C1s subcomponent (C1S), and C4b-binding protein alpha chain (C4BPA) was increased in the KD group (<i>P</i><0.05). Compared to the KD group, the expression of KNG1, C1S, C1QC, and C4BPA was decreased in the BBR treatment group (<i>P</i><0.05).</p><p><strong>Conclusions: </strong>The complement and coagulation cascade may be involved in the regulation of BBR treatment for coronary injury in KD, and C1QC, KNG1, C1S, and C4BPA may serve as biomarkers for this treatment.</p>","PeriodicalId":39792,"journal":{"name":"中国当代儿科杂志","volume":"27 1","pages":"101-108"},"PeriodicalIF":0.0,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Development of a predictive scoring model for non-response to intravenous immunoglobulin in Kawasaki disease]. [川崎病静脉注射免疫球蛋白无反应的预测评分模型的建立]。
Q3 Medicine Pub Date : 2025-01-15 DOI: 10.7499/j.issn.1008-8830.2408077
Yi-Xu Huang, Yu Huang, Guang-Huan Pi

Objectives: To explore the predictive factors for non-response to intravenous immunoglobulin (IVIG) in children with Kawasaki disease (KD) and to establish an IVIG non-response prediction scoring model for the Sichuan region.

Methods: A retrospective study was conducted by collecting clinical data from children with KD admitted to four tertiary hospitals in Sichuan Province between 2019 and 2023. Among them, 940 children responded to IVIG, while 74 children did not respond. Multivariate logistic regression analysis was used to identify the predictive factors for non-response to IVIG and to establish a predictive scoring model. The model's effectiveness was assessed using the receiver operating characteristic curve (ROC) and validated with an independent dataset.

Results: Multivariate logistic regression analysis showed that the platelet-to-lymphocyte ratio (PLR), hemoglobin (Hb), serum creatinine, aspartate aminotransferase (AST), and platelet count (PLT) were closely related to non-response to IVIG in children with KD (P<0.05). Based on these indicators, a predictive scoring model was established: PLR > 199, 0.4 points; Hb ≤ 116 g/L, 4 points; AST > 58 U/L, 0.2 points; serum creatinine > 38 µmol/L, 3.9 points; PLT count ≤ 275 × 109/L, 0.3 points. Using this model, children with KD were scored, and a total score greater than 4.3 was considered high risk of non-response to IVIG. The sensitivity of the model in predicting non-response to IVIG was 77.0%, specificity was 65.7%, and the area under the ROC curve was 0.746 (95%CI: 0.688-0.805).

Conclusions: The predictive scoring model based on PLR, Hb, serum creatinine, AST, and PLT demonstrates good predictive performance for non-response to IVIG in children with KD in the Sichuan region and can serve as a reference for clinical decision-making.

目的:探讨川崎病(KD)患儿静脉注射免疫球蛋白(IVIG)无反应的预测因素,建立适用于四川地区的IVIG无反应预测评分模型。方法:收集2019 - 2023年四川省四所三级医院收治的KD患儿临床资料,进行回顾性研究。其中940名儿童对IVIG有反应,74名儿童没有反应。采用多因素logistic回归分析,确定IVIG无反应的预测因素,建立预测评分模型。使用受试者工作特征曲线(ROC)评估模型的有效性,并使用独立数据集进行验证。结果:多因素logistic回归分析显示,血小板/淋巴细胞比(PLR)、血红蛋白(Hb)、血清肌酐、天冬氨酸转氨酶(AST)、血小板计数(PLT)与KD患儿IVIG无应答密切相关(P 199, 0.4分;Hb≤116 g/L, 4分;AST > 58 U/L, 0.2分;血清肌酐> 38µmol/L, 3.9分;PLT计数≤275 × 109/L, 0.3分。使用该模型对KD患儿进行评分,总分大于4.3分被认为是IVIG无反应的高风险。该模型预测IVIG无反应的敏感性为77.0%,特异性为65.7%,ROC曲线下面积为0.746 (95%CI: 0.688-0.805)。结论:基于PLR、Hb、血清肌酐、AST、PLT的预测评分模型对四川地区KD患儿IVIG无应答具有较好的预测效果,可作为临床决策参考。
{"title":"[Development of a predictive scoring model for non-response to intravenous immunoglobulin in Kawasaki disease].","authors":"Yi-Xu Huang, Yu Huang, Guang-Huan Pi","doi":"10.7499/j.issn.1008-8830.2408077","DOIUrl":"10.7499/j.issn.1008-8830.2408077","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the predictive factors for non-response to intravenous immunoglobulin (IVIG) in children with Kawasaki disease (KD) and to establish an IVIG non-response prediction scoring model for the Sichuan region.</p><p><strong>Methods: </strong>A retrospective study was conducted by collecting clinical data from children with KD admitted to four tertiary hospitals in Sichuan Province between 2019 and 2023. Among them, 940 children responded to IVIG, while 74 children did not respond. Multivariate logistic regression analysis was used to identify the predictive factors for non-response to IVIG and to establish a predictive scoring model. The model's effectiveness was assessed using the receiver operating characteristic curve (ROC) and validated with an independent dataset.</p><p><strong>Results: </strong>Multivariate logistic regression analysis showed that the platelet-to-lymphocyte ratio (PLR), hemoglobin (Hb), serum creatinine, aspartate aminotransferase (AST), and platelet count (PLT) were closely related to non-response to IVIG in children with KD (<i>P</i><0.05). Based on these indicators, a predictive scoring model was established: PLR > 199, 0.4 points; Hb ≤ 116 g/L, 4 points; AST > 58 U/L, 0.2 points; serum creatinine > 38 µmol/L, 3.9 points; PLT count ≤ 275 × 10<sup>9</sup>/L, 0.3 points. Using this model, children with KD were scored, and a total score greater than 4.3 was considered high risk of non-response to IVIG. The sensitivity of the model in predicting non-response to IVIG was 77.0%, specificity was 65.7%, and the area under the ROC curve was 0.746 (95%<i>CI</i>: 0.688-0.805).</p><p><strong>Conclusions: </strong>The predictive scoring model based on PLR, Hb, serum creatinine, AST, and PLT demonstrates good predictive performance for non-response to IVIG in children with KD in the Sichuan region and can serve as a reference for clinical decision-making.</p>","PeriodicalId":39792,"journal":{"name":"中国当代儿科杂志","volume":"27 1","pages":"75-81"},"PeriodicalIF":0.0,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Clinical characteristics and long-term follow-up study of basal ganglia infarction after minor head trauma in infants and young children]. [婴幼儿轻微头部外伤后基底神经节梗死的临床特点及长期随访研究]。
Q3 Medicine Pub Date : 2025-01-15 DOI: 10.7499/j.issn.1008-8830.2408136
Huan Xu, Chen-Chen Wu, Ji-Hong Tang, Jun Feng, Xiao Xiao, Xiao-Yan Shi, Dao-Qi Mei

Objectives: To investigate the clinical characteristics and prognosis of infants and young children with basal ganglia infarction after minor head trauma (BGIMHT).

Methods: A retrospective analysis was conducted on the clinical data and follow-up results of children aged 28 days to 3 years with BGIMHT who were hospitalized at Children's Hospital of Soochow University from January 2011 to January 2022.

Results: A total of 45 cases of BGIMHT were included, with the most common symptom being limb movement disorders (96%, 43/45), followed by facioplegia (56%, 25/45). Cerebral imaging showed that 72% (31/43) had infarction accompanied by basal ganglia calcification. After conservative treatment, 42 children (93%) showed significant symptom improvement, while 3 children (7%) experienced recurrent strokes. The median follow-up time was 82 months (range: 17-141 months). At the last follow-up, 97% (29/30) had residual basal ganglia softening lesions. Among 29 cases participating in questionnaire follow-up, 66% (19/29) recovered normally, 17% (5/29) showed significant improvement in symptoms, and 17% (5/29) had poor improvement. According to the grading of the Global Burden of Disease Control Projects, only 1 child (3%) had severe sequelae. There were no significant differences in age at onset, gender, or presence of concomitant basal ganglia calcification between children with and without neurological sequelae (P>0.05).

Conclusions: The most common initial symptom of BGIMHT is limb movement disorder, and imaging results indicate that most children have concurrent intracranial calcifications. Most infarct lesions later transform into softening lesions, resulting in a generally good prognosis.

目的:探讨婴幼儿颅脑外伤后基底神经节梗死的临床特点及预后。方法:回顾性分析2011年1月至2022年1月苏州大学儿童医院收治的28天~ 3岁BGIMHT患儿的临床资料及随访结果。结果:共纳入45例BGIMHT,最常见的症状为肢体运动障碍(96%,43/45),其次为面部截瘫(56%,25/45)。脑显像显示72%(31/43)脑梗死伴基底节区钙化。保守治疗后,42例患儿(93%)症状明显改善,3例患儿(7%)卒中复发。中位随访时间82个月(范围17-141个月)。在最后一次随访中,97%(29/30)有残留的基底节区软化病变。参与问卷随访的29例患者中,66%(19/29)恢复正常,17%(5/29)症状明显改善,17%(5/29)症状改善不佳。根据全球疾病控制项目负担的分级,只有1名儿童(3%)有严重的后遗症。有和无神经系统后遗症的儿童在发病年龄、性别或是否存在伴发基底神经节钙化方面无显著差异(P < 0.05)。结论:BGIMHT最常见的首发症状为肢体运动障碍,影像学结果显示多数患儿伴有颅内钙化。大多数梗死灶后来转化为软化灶,预后一般较好。
{"title":"[Clinical characteristics and long-term follow-up study of basal ganglia infarction after minor head trauma in infants and young children].","authors":"Huan Xu, Chen-Chen Wu, Ji-Hong Tang, Jun Feng, Xiao Xiao, Xiao-Yan Shi, Dao-Qi Mei","doi":"10.7499/j.issn.1008-8830.2408136","DOIUrl":"10.7499/j.issn.1008-8830.2408136","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the clinical characteristics and prognosis of infants and young children with basal ganglia infarction after minor head trauma (BGIMHT).</p><p><strong>Methods: </strong>A retrospective analysis was conducted on the clinical data and follow-up results of children aged 28 days to 3 years with BGIMHT who were hospitalized at Children's Hospital of Soochow University from January 2011 to January 2022.</p><p><strong>Results: </strong>A total of 45 cases of BGIMHT were included, with the most common symptom being limb movement disorders (96%, 43/45), followed by facioplegia (56%, 25/45). Cerebral imaging showed that 72% (31/43) had infarction accompanied by basal ganglia calcification. After conservative treatment, 42 children (93%) showed significant symptom improvement, while 3 children (7%) experienced recurrent strokes. The median follow-up time was 82 months (range: 17-141 months). At the last follow-up, 97% (29/30) had residual basal ganglia softening lesions. Among 29 cases participating in questionnaire follow-up, 66% (19/29) recovered normally, 17% (5/29) showed significant improvement in symptoms, and 17% (5/29) had poor improvement. According to the grading of the Global Burden of Disease Control Projects, only 1 child (3%) had severe sequelae. There were no significant differences in age at onset, gender, or presence of concomitant basal ganglia calcification between children with and without neurological sequelae (<i>P</i>>0.05).</p><p><strong>Conclusions: </strong>The most common initial symptom of BGIMHT is limb movement disorder, and imaging results indicate that most children have concurrent intracranial calcifications. Most infarct lesions later transform into softening lesions, resulting in a generally good prognosis.</p>","PeriodicalId":39792,"journal":{"name":"中国当代儿科杂志","volume":"27 1","pages":"68-74"},"PeriodicalIF":0.0,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Clinical practice guidelines for the diagnosis and treatment of anemia of prematurity (2025)]. 【早产儿贫血诊治临床实践指南(2025)】。
Q3 Medicine Pub Date : 2025-01-15 DOI: 10.7499/j.issn.1008-8830.2407094

Anemia of prematurity (AOP) is a multifactorial condition associated with congenital iron deficiency, low erythropoietin levels, a short lifespan of red blood cells, and iatrogenic blood loss. AOP is a common complication in premature infants that can adversely affect growth, development, and long-term neurocognitive outcomes. To standardize the diagnosis and treatment of AOP, the Neonatal Clinical Practice Guidelines Expert Committee and the Neonatal Evidence-Based Medicine Group of the Commission of Neonatal Medicine of the Cross-Strait Medical and Health Exchange Association, along with the Editorial Office of the Chinese Journal of Contemporary Pediatrics, have developed the "Clinical practice guidelines for the diagnosis and treatment of anemia of prematurity (2025)", based on the World Health Organization's handbook for guideline development and the formulation/revision principles of Chinese clinical practice guidelines. This guideline addresses eight clinical issues related to AOP, including risk factors, early identification, etiological diagnosis, diagnostic criteria, early prevention, transfusion therapy, strategies to improve prognosis, and post-discharge follow-up. It presents 29 recommendations formed from current evidence and expert consensus, aiming to provide guidance and decision-making support for healthcare professionals in the diagnosis and treatment of AOP.

早产儿贫血(AOP)是一种多因素疾病,与先天性缺铁、低促红细胞生成素水平、红细胞寿命短和医源性失血有关。AOP是早产儿中一种常见的并发症,可以对生长、发育和长期神经认知结果产生不利影响。为规范AOP的诊治,海峡两岸医疗卫生交流协会新生儿医学专业委员会新生儿临床实践指南专家委员会、新生儿循证医学专家组与《中国当代儿科学杂志》编辑部共同制定了《早产儿贫血诊治临床实践指南(2025)》。依据世界卫生组织指南制定手册和中国临床实践指南制定/修订原则。本指南涉及与AOP相关的八个临床问题,包括危险因素、早期识别、病因诊断、诊断标准、早期预防、输血治疗、改善预后的策略和出院后随访。根据现有证据和专家共识提出了29条建议,旨在为医疗保健专业人员在AOP的诊断和治疗方面提供指导和决策支持。
{"title":"[Clinical practice guidelines for the diagnosis and treatment of anemia of prematurity (2025)].","authors":"","doi":"10.7499/j.issn.1008-8830.2407094","DOIUrl":"10.7499/j.issn.1008-8830.2407094","url":null,"abstract":"<p><p>Anemia of prematurity (AOP) is a multifactorial condition associated with congenital iron deficiency, low erythropoietin levels, a short lifespan of red blood cells, and iatrogenic blood loss. AOP is a common complication in premature infants that can adversely affect growth, development, and long-term neurocognitive outcomes. To standardize the diagnosis and treatment of AOP, the Neonatal Clinical Practice Guidelines Expert Committee and the Neonatal Evidence-Based Medicine Group of the Commission of Neonatal Medicine of the Cross-Strait Medical and Health Exchange Association, along with the Editorial Office of the <i>Chinese Journal of Contemporary Pediatrics</i>, have developed the \"Clinical practice guidelines for the diagnosis and treatment of anemia of prematurity (2025)\", based on the World Health Organization's handbook for guideline development and the formulation/revision principles of Chinese clinical practice guidelines. This guideline addresses eight clinical issues related to AOP, including risk factors, early identification, etiological diagnosis, diagnostic criteria, early prevention, transfusion therapy, strategies to improve prognosis, and post-discharge follow-up. It presents 29 recommendations formed from current evidence and expert consensus, aiming to provide guidance and decision-making support for healthcare professionals in the diagnosis and treatment of AOP.</p>","PeriodicalId":39792,"journal":{"name":"中国当代儿科杂志","volume":"27 1","pages":"1-17"},"PeriodicalIF":0.0,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Explanation and interpretation of blood transfusion provisions for children with hematological diseases in the national health standard "Guideline for pediatric transfusion"]. 【对国家卫生标准《小儿输血指南》中血液病患儿输血规定的说明和解读】。
Q3 Medicine Pub Date : 2025-01-15 DOI: 10.7499/j.issn.1008-8830.2410093
Ming-Yi Zhao, Rong Huang, Rong Gui, Qing-Nan He, Ming-Yan Hei, Xiao-Fan Zhu, Jun Lu, Xiao-Jun Xu, Tian-Ming Yuan, Rong Zhang, Xu Wang, Jin-Ping Liu, Jing Wang, Zhi-Li Shao, Yong-Jian Guo, Xin-Yin Wu, Jia-Rui Chen, Qi-Rong Chen, Jia Guo, Ming-Hua Yang

To guide clinical blood transfusion practices for pediatric patients, the National Health Commission has issued the health standard "Guideline for pediatric transfusion" (WS/T 795-2022). Blood transfusion is one of the most commonly used supportive treatments for children with hematological diseases. This guideline provides guidance and recommendations for blood transfusions in children with aplastic anemia, thalassemia, autoimmune hemolytic anemia, glucose-6-phosphate dehydrogenase deficiency, acute leukemia, myelodysplastic syndromes, immune thrombocytopenic purpura, and thrombotic thrombocytopenic purpura. This article presents the evidence and interpretation of the blood transfusion provisions for children with hematological diseases in the "Guideline for pediatric transfusion", aiming to assist in the understanding and implementing the blood transfusion section of this guideline.

为指导儿科患者的临床输血操作,国家卫健委发布了卫生标准《儿科输血指南》(WS/T 795-2022)。输血是儿童血液病最常用的支持性治疗方法之一。本指南为再生障碍性贫血、地中海贫血、自身免疫性溶血性贫血、葡萄糖-6-磷酸脱氢酶缺乏症、急性白血病、骨髓增生异常综合征、免疫性血小板减少性紫癜和血栓性血小板减少性紫癜患儿输血提供指导和建议。本文通过对《儿科输血指南》中血液病患儿输血规定的证据和解读,旨在帮助理解和实施该指南中输血部分。
{"title":"[Explanation and interpretation of blood transfusion provisions for children with hematological diseases in the national health standard \"Guideline for pediatric transfusion\"].","authors":"Ming-Yi Zhao, Rong Huang, Rong Gui, Qing-Nan He, Ming-Yan Hei, Xiao-Fan Zhu, Jun Lu, Xiao-Jun Xu, Tian-Ming Yuan, Rong Zhang, Xu Wang, Jin-Ping Liu, Jing Wang, Zhi-Li Shao, Yong-Jian Guo, Xin-Yin Wu, Jia-Rui Chen, Qi-Rong Chen, Jia Guo, Ming-Hua Yang","doi":"10.7499/j.issn.1008-8830.2410093","DOIUrl":"10.7499/j.issn.1008-8830.2410093","url":null,"abstract":"<p><p>To guide clinical blood transfusion practices for pediatric patients, the National Health Commission has issued the health standard \"Guideline for pediatric transfusion\" (WS/T 795-2022). Blood transfusion is one of the most commonly used supportive treatments for children with hematological diseases. This guideline provides guidance and recommendations for blood transfusions in children with aplastic anemia, thalassemia, autoimmune hemolytic anemia, glucose-6-phosphate dehydrogenase deficiency, acute leukemia, myelodysplastic syndromes, immune thrombocytopenic purpura, and thrombotic thrombocytopenic purpura. This article presents the evidence and interpretation of the blood transfusion provisions for children with hematological diseases in the \"Guideline for pediatric transfusion\", aiming to assist in the understanding and implementing the blood transfusion section of this guideline.</p>","PeriodicalId":39792,"journal":{"name":"中国当代儿科杂志","volume":"27 1","pages":"18-25"},"PeriodicalIF":0.0,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750235/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Risk factors for plastic bronchitis in children with macrolide-unresponsive Mycoplasma pneumoniae pneumonia and establishment of a nomogram model]. [大环内酯无反应肺炎支原体肺炎患儿塑性支气管炎的危险因素及nomogram模型的建立]。
Q3 Medicine Pub Date : 2025-01-15 DOI: 10.7499/j.issn.1008-8830.2408073
Xiao-Song Shi, Xiao-Hua He, Jie Chen

Objectives: To investigate the risk factors for plastic bronchitis (PB) in children with macrolide-unresponsive Mycoplasma pneumoniae pneumonia (MUMPP) and to establish a nomogram prediction model.

Methods: A retrospective analysis was conducted on 178 children with MUMPP who underwent bronchoscopy from January to December 2023. According to the presence or absence of PB, the children were divided into a PB group (49 children) and a non-PB group (129 children). The predictive factors for the development of PB in children with MUMPP were analyzed, and a nomogram prediction model was established. The model was assessed in terms of discriminatory ability, accuracy, and clinical effectiveness.

Results: The multivariate logistic regression analysis showed that older age and higher levels of lactate dehydrogenase and fibrinogen were closely associated with the development of PB in children with MUMPP (P<0.05). A nomogram model established based on these factors had an area under the receiver operating characteristic curve of 0.733 (95%CI: 0.651-0.816, P<0.001) and showed a good discriminatory ability. The Hosmer-Lemeshow goodness-of-fit test indicated that the predictive model had a good degree of fit (P>0.05), and the decision curve analysis showed that the model had a good clinical application value.

Conclusions: The risk nomogram model established based on age and lactate dehydrogenase and fibrinogen levels has good discriminatory ability, accuracy, and predictive efficacy for predicting the development of PB in children with MUMPP.

目的:探讨大环内酯无反应性肺炎支原体肺炎(MUMPP)患儿塑性支气管炎(PB)的危险因素,并建立模型预测模型。方法:对2023年1 - 12月行支气管镜检查的178例MUMPP患儿进行回顾性分析。根据是否存在PB,将儿童分为PB组(49名)和非PB组(129名)。分析MUMPP患儿PB发展的预测因素,建立nomogram预测模型。评估该模型的区分能力、准确性和临床有效性。结果:多因素logistic回归分析显示,年龄越大、乳酸脱氢酶和纤维蛋白原水平越高与MUMPP患儿PB的发生密切相关(PCI: 0.651-0.816, PP>0.05),决策曲线分析表明该模型具有较好的临床应用价值。结论:基于年龄、乳酸脱氢酶和纤维蛋白原水平建立的风险nomogram模型对预测MUMPP患儿PB的发展具有良好的鉴别能力、准确性和预测效果。
{"title":"[Risk factors for plastic bronchitis in children with macrolide-unresponsive <i>Mycoplasma pneumoniae</i> pneumonia and establishment of a nomogram model].","authors":"Xiao-Song Shi, Xiao-Hua He, Jie Chen","doi":"10.7499/j.issn.1008-8830.2408073","DOIUrl":"10.7499/j.issn.1008-8830.2408073","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the risk factors for plastic bronchitis (PB) in children with macrolide-unresponsive <i>Mycoplasma pneumoniae</i> pneumonia (MUMPP) and to establish a nomogram prediction model.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 178 children with MUMPP who underwent bronchoscopy from January to December 2023. According to the presence or absence of PB, the children were divided into a PB group (49 children) and a non-PB group (129 children). The predictive factors for the development of PB in children with MUMPP were analyzed, and a nomogram prediction model was established. The model was assessed in terms of discriminatory ability, accuracy, and clinical effectiveness.</p><p><strong>Results: </strong>The multivariate logistic regression analysis showed that older age and higher levels of lactate dehydrogenase and fibrinogen were closely associated with the development of PB in children with MUMPP (<i>P</i><0.05). A nomogram model established based on these factors had an area under the receiver operating characteristic curve of 0.733 (95%<i>CI</i>: 0.651-0.816, <i>P</i><0.001) and showed a good discriminatory ability. The Hosmer-Lemeshow goodness-of-fit test indicated that the predictive model had a good degree of fit (<i>P</i>>0.05), and the decision curve analysis showed that the model had a good clinical application value.</p><p><strong>Conclusions: </strong>The risk nomogram model established based on age and lactate dehydrogenase and fibrinogen levels has good discriminatory ability, accuracy, and predictive efficacy for predicting the development of PB in children with MUMPP.</p>","PeriodicalId":39792,"journal":{"name":"中国当代儿科杂志","volume":"27 1","pages":"62-67"},"PeriodicalIF":0.0,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Advances in the application of comprehensive behavioral intervention in tic disorder]. 综合行为干预在抽动障碍中的应用进展
Q3 Medicine Pub Date : 2024-12-15 DOI: 10.7499/j.issn.1008-8830.2407171
Sai Fu, Qian Song, Xiang-Jun He, Xiao-Yu Tian

Tic disorder is a neurodevelopmental disorder that occurs in children or adolescents, often attracting the attention of others due to involuntary, repetitive, and non-rhythmic tics, and drug therapy often causes negative emotions in children and their families due to its significant adverse reactions, poor compliance, and tendency of recurrence after drug withdrawal. In recent years, comprehensive behavioral intervention has shown great potential as a safe and effective treatment modality for tic disorders, with few adverse reactions. This article reviews the advances in the application of comprehensive behavioral intervention for tic disorder in China and abroad in the past 5 years, in order to provide a reference for clinical application.

抽动障碍是一种发生于儿童或青少年的神经发育障碍,常因不自主的、重复的、无节奏的抽动而引起他人的注意,药物治疗因其不良反应显著、依从性差、停药后有复发倾向,常引起儿童及其家庭的负面情绪。近年来,综合行为干预作为一种安全有效、不良反应少的抽动障碍治疗方式显示出巨大的潜力。本文综述了近5年来国内外抽动障碍综合行为干预的应用进展,以期为临床应用提供参考。
{"title":"[Advances in the application of comprehensive behavioral intervention in tic disorder].","authors":"Sai Fu, Qian Song, Xiang-Jun He, Xiao-Yu Tian","doi":"10.7499/j.issn.1008-8830.2407171","DOIUrl":"10.7499/j.issn.1008-8830.2407171","url":null,"abstract":"<p><p>Tic disorder is a neurodevelopmental disorder that occurs in children or adolescents, often attracting the attention of others due to involuntary, repetitive, and non-rhythmic tics, and drug therapy often causes negative emotions in children and their families due to its significant adverse reactions, poor compliance, and tendency of recurrence after drug withdrawal. In recent years, comprehensive behavioral intervention has shown great potential as a safe and effective treatment modality for tic disorders, with few adverse reactions. This article reviews the advances in the application of comprehensive behavioral intervention for tic disorder in China and abroad in the past 5 years, in order to provide a reference for clinical application.</p>","PeriodicalId":39792,"journal":{"name":"中国当代儿科杂志","volume":"26 12","pages":"1367-1372"},"PeriodicalIF":0.0,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11684835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Clinical characteristics and prognosis of children with T-lineage acute lymphoblastic leukemia: a single-center study]. 儿童t系急性淋巴细胞白血病的临床特征和预后:一项单中心研究。
Q3 Medicine Pub Date : 2024-12-15 DOI: 10.7499/j.issn.1008-8830.2408039
Xiao-Yan Chen, Jia-Yi Wang, Hua Jiang, Wei-Na Zhang

Objectives: To study the clinical characteristics and prognosis of T-lineage acute lymphoblastic leukemia (T-ALL) and related prognostic factors.

Methods: A retrospective analysis was conducted on the children with T-ALL who were treated with the Chinese Children's Cancer Group Acute Lymphoblastic Leukemia (CCCG-ALL) regimen in Guangzhou Women and Children's Medical Center between April 2015 and December 2022.

Results: A total of 80 children were included, with a median age of 7 years and 3 months and a male/female ratio of 6:1. Among these children, the children with mediastinal mass accounted for 20% (16/80), those with central nervous system leukemia accounted for 4% (3/80), and those with testicular leukemia accounted for 1% (1/69). SIL/TAL1 was the most common fusion gene (22%, 18/80), and NOTCH1 was the most common mutation gene (69%, 37/54). The median follow-up time was 52 months, with a 5-year overall survival (OS) rate of 87.3%±4.0% and a 5-year event-free survival rate of 84.0%±4.3%. The non-central nervous system-1 group had a significantly lower 5-year OS rate than the central nervous system-1 group (66.7%±16.1% vs 90.3%±3.8%; P<0.05), and the group with minimal residual disease (MRD) ≥0.01% on day 46 of induction therapy had a significantly lower 5-year OS rate than the group with MRD <0.01% (68.6%±13.5% vs 94.8%±3.0%; P<0.05).

Conclusions: Children treated with the CCCG-ALL regimen tend to have a good treatment outcome. Non-central nervous system-1 status and MRD ≥0.01% on day 46 of induction therapy are associated with the poor prognosis in these children.

目的:探讨t系急性淋巴细胞白血病(T-ALL)的临床特点、预后及相关影响因素。方法:回顾性分析2015年4月至2022年12月在广州市妇女儿童医疗中心接受中国儿童肿瘤组急性淋巴母细胞白血病(CCCG-ALL)方案治疗的T-ALL患儿。结果:共纳入患儿80例,中位年龄7岁3个月,男女比例为6:1。其中纵隔肿块患儿占20%(16/80),中枢神经系统白血病患儿占4%(3/80),睾丸白血病患儿占1%(1/69)。SIL/TAL1是最常见的融合基因(22%,18/80),NOTCH1是最常见的突变基因(69%,37/54)。中位随访时间为52个月,5年总生存率(OS)为87.3%±4.0%,5年无事件生存率为84.0%±4.3%。非中枢神经系统-1组5年OS率显著低于中枢神经系统-1组(66.7%±16.1% vs 90.3%±3.8%;结论:CCCG-ALL方案患儿治疗效果较好。诱导治疗第46天非中枢神经系统-1状态和MRD≥0.01%与这些患儿预后不良相关。
{"title":"[Clinical characteristics and prognosis of children with T-lineage acute lymphoblastic leukemia: a single-center study].","authors":"Xiao-Yan Chen, Jia-Yi Wang, Hua Jiang, Wei-Na Zhang","doi":"10.7499/j.issn.1008-8830.2408039","DOIUrl":"10.7499/j.issn.1008-8830.2408039","url":null,"abstract":"<p><strong>Objectives: </strong>To study the clinical characteristics and prognosis of T-lineage acute lymphoblastic leukemia (T-ALL) and related prognostic factors.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on the children with T-ALL who were treated with the Chinese Children's Cancer Group Acute Lymphoblastic Leukemia (CCCG-ALL) regimen in Guangzhou Women and Children's Medical Center between April 2015 and December 2022.</p><p><strong>Results: </strong>A total of 80 children were included, with a median age of 7 years and 3 months and a male/female ratio of 6:1. Among these children, the children with mediastinal mass accounted for 20% (16/80), those with central nervous system leukemia accounted for 4% (3/80), and those with testicular leukemia accounted for 1% (1/69). <i>SIL/TAL1</i> was the most common fusion gene (22%, 18/80), and <i>NOTCH1</i> was the most common mutation gene (69%, 37/54). The median follow-up time was 52 months, with a 5-year overall survival (OS) rate of 87.3%±4.0% and a 5-year event-free survival rate of 84.0%±4.3%. The non-central nervous system-1 group had a significantly lower 5-year OS rate than the central nervous system-1 group (66.7%±16.1% vs 90.3%±3.8%; <i>P</i><0.05), and the group with minimal residual disease (MRD) ≥0.01% on day 46 of induction therapy had a significantly lower 5-year OS rate than the group with MRD <0.01% (68.6%±13.5% vs 94.8%±3.0%; <i>P</i><0.05).</p><p><strong>Conclusions: </strong>Children treated with the CCCG-ALL regimen tend to have a good treatment outcome. Non-central nervous system-1 status and MRD ≥0.01% on day 46 of induction therapy are associated with the poor prognosis in these children.</p>","PeriodicalId":39792,"journal":{"name":"中国当代儿科杂志","volume":"26 12","pages":"1308-1314"},"PeriodicalIF":0.0,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11684830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Role of the nuclear factor-kappa B signaling pathway in the repair of white matter injury in neonatal rats through human umbilical cord mesenchymal stem cell transplantation]. [核因子- κ B信号通路在人脐带间充质干细胞移植修复新生大鼠白质损伤中的作用]。
Q3 Medicine Pub Date : 2024-12-15 DOI: 10.7499/j.issn.1008-8830.2408099
Shu-Juan Zhang, Chao Wang, Qian-Qian Xu, Jun Zhang, Yan-Ping Zhu
<p><strong>Objectives: </strong>To observe the reparative effects of human umbilical cord mesenchymal stem cell (hUC-MSC) transplantation on white matter injury (WMI) in neonatal rats and explore its mechanism through the nuclear factor-kappa B (NF-κB) signaling pathway mediated by microglial cells.</p><p><strong>Methods: </strong>Sprague-Dawley rats, aged 2 days, were randomly divided into three groups: sham-operation,WMI, and hUC-MSC (<i>n</i>=18 each). Fourteen days after modeling, hematoxylin-eosin staining was used to observe pathological changes in the white matter, and immunofluorescence staining was used to measure the expression level of ionized calcium-binding adapter molecule 1 (Iba1). Western blotting was used to measure the protein expression levels of inhibitory subunit of nuclear factor-kappa B alpha (IκBα), phosphorylated IκBα (p-IκBα), phosphorylated NF-κB p65 (p-NF-κB p65), myelin basic protein (MBP), and neuron-specific nuclear protein (NeuN). Quantitative real-time PCR was used to assess the mRNA expression levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), MBP, and NeuN. Immunohistochemistry was used to measure the protein expression levels of MBP and NeuN. On day 28, the Morris water maze test was used to evaluate spatial cognitive ability.</p><p><strong>Results: </strong>Fourteen days after modeling, the sham-operation group exhibited intact white matter structure with normal cell morphology and orderly nerve fiber arrangement. In the WMI group, large-scale cell degeneration and necrosis were observed, and nerve fiber arrangement was disordered. The hUC-MSC group showed relatively normal cell morphology and more orderly nerve fibers. Compared with the sham-operation group, the WMI group had significantly higher proportions of Iba1-positive cells, increased protein levels of p-IκBα and p-NF-κB p65, and higher mRNA levels of TNF-α and IL-1β. The protein expression of IκBα and the positive expression of MBP and NeuN, as well as their protein and mRNA levels, were significantly reduced in the WMI group (<i>P</i><0.05). Compared with the WMI group, the hUC-MSC group showed reduced proportions of Iba1-positive cells, decreased protein levels of p-IκBα and p-NF-κB p65, and lower mRNA levels of TNF-α and IL-1β. Furthermore, IκBα protein expression and MBP and NeuN expression (both at the protein and mRNA levels) were significantly increased in the hUC-MSC group (<i>P</i><0.05). On day 28, the Morris water maze results showed that compared with the sham-operation group, the WMI group had significantly longer escape latency and fewer platform crossings (<i>P</i><0.05). In contrast, the hUC-MSC group had significantly shorter escape latency and more platform crossings than the WMI group (<i>P</i><0.05).</p><p><strong>Conclusions: </strong>hUC-MSC transplantation can repair WMI in neonatal rats, promote the maturation of oligodendrocytes, and support neuronal survival, likely by inhibiting activation of the NF-κB signal
目的:观察人脐带间充质干细胞(hUC-MSC)移植对新生大鼠白质损伤(WMI)的修复作用,并通过小胶质细胞介导的核因子-κB (NF-κB)信号通路探讨其机制。方法:取2日龄的Sprague-Dawley大鼠,随机分为假手术组、WMI组和hUC-MSC组,每组18只。造模后第14天,采用苏木精-伊红染色观察大鼠白质的病理变化,免疫荧光染色检测离子化钙结合转接器分子1 (Iba1)的表达水平。Western blotting检测核因子κBα抑制亚基(IκBα)、磷酸化i -κB α (p- i -κB α)、磷酸化NF-κB p65 (p-NF-κB p65)、髓鞘碱性蛋白(MBP)、神经元特异性核蛋白(NeuN)的蛋白表达水平。采用实时荧光定量PCR检测肿瘤坏死因子-α (TNF-α)、白细胞介素-1β (IL-1β)、MBP、NeuN mRNA表达水平。免疫组化法检测MBP和NeuN蛋白表达水平。第28天采用Morris水迷宫试验评价空间认知能力。结果:造模后第14天,假手术组脑白质结构完整,细胞形态正常,神经纤维排列有序。WMI组大范围细胞变性坏死,神经纤维排列紊乱。hUC-MSC组细胞形态相对正常,神经纤维较为有序。与假手术组相比,WMI组iba1阳性细胞比例显著升高,p -κB α和p-NF-κB p65蛋白水平升高,TNF-α和IL-1β mRNA水平升高。结论:hUC-MSC移植可能通过抑制小胶质细胞介导的NF-κB信号通路的激活来修复新生大鼠WMI,促进少突胶质细胞成熟,支持神经元存活。短句来源
{"title":"[Role of the nuclear factor-kappa B signaling pathway in the repair of white matter injury in neonatal rats through human umbilical cord mesenchymal stem cell transplantation].","authors":"Shu-Juan Zhang, Chao Wang, Qian-Qian Xu, Jun Zhang, Yan-Ping Zhu","doi":"10.7499/j.issn.1008-8830.2408099","DOIUrl":"10.7499/j.issn.1008-8830.2408099","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To observe the reparative effects of human umbilical cord mesenchymal stem cell (hUC-MSC) transplantation on white matter injury (WMI) in neonatal rats and explore its mechanism through the nuclear factor-kappa B (NF-κB) signaling pathway mediated by microglial cells.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Sprague-Dawley rats, aged 2 days, were randomly divided into three groups: sham-operation,WMI, and hUC-MSC (&lt;i&gt;n&lt;/i&gt;=18 each). Fourteen days after modeling, hematoxylin-eosin staining was used to observe pathological changes in the white matter, and immunofluorescence staining was used to measure the expression level of ionized calcium-binding adapter molecule 1 (Iba1). Western blotting was used to measure the protein expression levels of inhibitory subunit of nuclear factor-kappa B alpha (IκBα), phosphorylated IκBα (p-IκBα), phosphorylated NF-κB p65 (p-NF-κB p65), myelin basic protein (MBP), and neuron-specific nuclear protein (NeuN). Quantitative real-time PCR was used to assess the mRNA expression levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), MBP, and NeuN. Immunohistochemistry was used to measure the protein expression levels of MBP and NeuN. On day 28, the Morris water maze test was used to evaluate spatial cognitive ability.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Fourteen days after modeling, the sham-operation group exhibited intact white matter structure with normal cell morphology and orderly nerve fiber arrangement. In the WMI group, large-scale cell degeneration and necrosis were observed, and nerve fiber arrangement was disordered. The hUC-MSC group showed relatively normal cell morphology and more orderly nerve fibers. Compared with the sham-operation group, the WMI group had significantly higher proportions of Iba1-positive cells, increased protein levels of p-IκBα and p-NF-κB p65, and higher mRNA levels of TNF-α and IL-1β. The protein expression of IκBα and the positive expression of MBP and NeuN, as well as their protein and mRNA levels, were significantly reduced in the WMI group (&lt;i&gt;P&lt;/i&gt;&lt;0.05). Compared with the WMI group, the hUC-MSC group showed reduced proportions of Iba1-positive cells, decreased protein levels of p-IκBα and p-NF-κB p65, and lower mRNA levels of TNF-α and IL-1β. Furthermore, IκBα protein expression and MBP and NeuN expression (both at the protein and mRNA levels) were significantly increased in the hUC-MSC group (&lt;i&gt;P&lt;/i&gt;&lt;0.05). On day 28, the Morris water maze results showed that compared with the sham-operation group, the WMI group had significantly longer escape latency and fewer platform crossings (&lt;i&gt;P&lt;/i&gt;&lt;0.05). In contrast, the hUC-MSC group had significantly shorter escape latency and more platform crossings than the WMI group (&lt;i&gt;P&lt;/i&gt;&lt;0.05).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;hUC-MSC transplantation can repair WMI in neonatal rats, promote the maturation of oligodendrocytes, and support neuronal survival, likely by inhibiting activation of the NF-κB signal","PeriodicalId":39792,"journal":{"name":"中国当代儿科杂志","volume":"26 12","pages":"1352-1361"},"PeriodicalIF":0.0,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11684829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Clinical and pathological features of children with immunoglobulin A vasculitis with nephritis accompanied by different proportions of crescent formation]. [免疫球蛋白A血管炎合并肾炎伴不同比例月牙形成的临床病理特点]。
Q3 Medicine Pub Date : 2024-12-15 DOI: 10.7499/j.issn.1008-8830.2407060
Meng-Ke Bai, Long Wang, Hang Li, Hang Su, Yue-Li Yang, Xiao-Qing Yang

Objectives: To investigate the impact of the different proportions of crescent formation on clinical manifestations and pathological features in children with immunoglobulin A vasculitis with nephritis (IgAVN).

Methods: The children with IgAVN were divided into no-crescent group (75 children), ≤25% crescent group (156 children), and >25% crescent group (33 children).

Results: Compared with the no-crescent group, the other two groups had significant increases in 24-hour urinary protein, urinary immunoglobulin G (IgG)/creatinine ratio, urine red blood cell count, fibrinogen, and neutrophil-lymphocyte ratio, a significant reduction in serum IgG, and a significantly higher proportion of children with low albumin and hypercoagulability, pathological grade III+IV or diffuse mesangial proliferation (P<0.05). Compared with the ≤25% crescent group, the >25% crescent group had significant increases in 24-hour urinary protein, urine red blood cell count, and fibrinogen, significant reductions in serum IgG and glomerular filtration rate, and a significantly higher proportion of children with diffuse mesangial proliferation, tubular atrophy or interstitial fibrosis (P<0.05). Compared with the no-crescent group, the >25% crescent group had significantly higher levels of total cholesterol, triglycerides, urea nitrogen, and serum creatinine (P<0.05). A reduction in serum IgG, hypercoagulability, an increase in 24-hour urinary protein, diffuse mesangial proliferation, and chronic tubulointerstitial lesions were influencing factors for the increase in the proportion of crescent formation (P<0.05).

Conclusions: For children with IgAVN, the higher proportion of crescent formation is associated with greater abnormalities in laboratory markers and more severe chronic tubulointerstitial lesions, and thus a detailed analysis of the proportion of crescent formation can better guide clinical treatment.

目的:探讨不同比例的新月形成对免疫球蛋白A血管炎合并肾炎(IgAVN)患儿临床表现和病理特征的影响。方法:将IgAVN患儿分为无月牙组(75例)、月牙≤25%组(156例)和月牙≤25%组(33例)。结果:与无新月组比较,其他两组24小时尿蛋白、尿免疫球蛋白G (IgG)/肌酐比值、尿红细胞计数、纤维蛋白原、中性粒细胞/淋巴细胞比值显著升高,血清IgG显著降低,低白蛋白、高凝性、病状III+IV级或弥漫性系膜增生患儿比例显著升高(P25%新月组24小时尿蛋白显著升高;尿红细胞计数、纤维蛋白原、血清IgG和肾小球滤过率显著降低,弥漫性系膜增生、小管萎缩或间质纤维化患儿比例显著升高(P25%新月组总胆固醇、甘油三酯、尿素氮和血清肌酐水平显著升高)。对于IgAVN患儿,月牙形成比例越高,实验室标志物异常越大,慢性小管间质病变越严重,详细分析月牙形成比例可以更好地指导临床治疗。
{"title":"[Clinical and pathological features of children with immunoglobulin A vasculitis with nephritis accompanied by different proportions of crescent formation].","authors":"Meng-Ke Bai, Long Wang, Hang Li, Hang Su, Yue-Li Yang, Xiao-Qing Yang","doi":"10.7499/j.issn.1008-8830.2407060","DOIUrl":"10.7499/j.issn.1008-8830.2407060","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the impact of the different proportions of crescent formation on clinical manifestations and pathological features in children with immunoglobulin A vasculitis with nephritis (IgAVN).</p><p><strong>Methods: </strong>The children with IgAVN were divided into no-crescent group (75 children), ≤25% crescent group (156 children), and >25% crescent group (33 children).</p><p><strong>Results: </strong>Compared with the no-crescent group, the other two groups had significant increases in 24-hour urinary protein, urinary immunoglobulin G (IgG)/creatinine ratio, urine red blood cell count, fibrinogen, and neutrophil-lymphocyte ratio, a significant reduction in serum IgG, and a significantly higher proportion of children with low albumin and hypercoagulability, pathological grade III+IV or diffuse mesangial proliferation (<i>P</i><0.05). Compared with the ≤25% crescent group, the >25% crescent group had significant increases in 24-hour urinary protein, urine red blood cell count, and fibrinogen, significant reductions in serum IgG and glomerular filtration rate, and a significantly higher proportion of children with diffuse mesangial proliferation, tubular atrophy or interstitial fibrosis (<i>P</i><0.05). Compared with the no-crescent group, the >25% crescent group had significantly higher levels of total cholesterol, triglycerides, urea nitrogen, and serum creatinine (<i>P</i><0.05). A reduction in serum IgG, hypercoagulability, an increase in 24-hour urinary protein, diffuse mesangial proliferation, and chronic tubulointerstitial lesions were influencing factors for the increase in the proportion of crescent formation (<i>P</i><0.05).</p><p><strong>Conclusions: </strong>For children with IgAVN, the higher proportion of crescent formation is associated with greater abnormalities in laboratory markers and more severe chronic tubulointerstitial lesions, and thus a detailed analysis of the proportion of crescent formation can better guide clinical treatment.</p>","PeriodicalId":39792,"journal":{"name":"中国当代儿科杂志","volume":"26 12","pages":"1329-1334"},"PeriodicalIF":0.0,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11684822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
中国当代儿科杂志
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1