Pub Date : 2025-04-17DOI: 10.1016/j.jhazmat.2025.138330
Hyun-Woo Kim , Jieun Lee , Seongmin Kim , Seung-Do Yun , Ki Jung Kim , Min-Ju Seo , Hyeoncheol Francis Son , Dong-Jun Lee , Chungoo Park , Won Seok Chi , Soo-Jin Yeom
Polycarbonate (PC), a widely used thermoplastic, poses significant environmental challenges due to its persistence and the release of bisphenol A (BPA), a known xenoestrogen. Here, we report the isolation of Bacillus subtilis JNU01 (BsJNU01), capable of utilizing PC as its sole carbon source. Through transcriptomic analysis, we identified metallophosphoesterase from BsJNU01 (BsMPPE), the first reported metallophosphoesterase capable of degrading polycarbonate by catalyzing the hydrolysis of carbonate ester bonds. This enzyme operates under mild aqueous conditions (30 °C, pH 7), releasing 30 μmol of BPA as a monomer and demonstrating effective PC degradation under environmentally friendly conditions. PC biodegradation was confirmed by Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), and gas chromatography-mass spectrometry (GC-MS). Furthermore, surface and mechanical analyses revealed significant degradation and structural changes in PC films following BsMPPE treatment, with toughness showing a 40–70 % decrease compared to untreated PC films. This study represents a breakthrough in microbial plastic degradation, establishing a sustainable biocatalytic platform for PC recycling and upcycling technologies.
{"title":"Exploring a Novel Metallophosphoesterase for Polycarbonate Degradation via Transcriptome Analysis","authors":"Hyun-Woo Kim , Jieun Lee , Seongmin Kim , Seung-Do Yun , Ki Jung Kim , Min-Ju Seo , Hyeoncheol Francis Son , Dong-Jun Lee , Chungoo Park , Won Seok Chi , Soo-Jin Yeom","doi":"10.1016/j.jhazmat.2025.138330","DOIUrl":"10.1016/j.jhazmat.2025.138330","url":null,"abstract":"<div><div>Polycarbonate (PC), a widely used thermoplastic, poses significant environmental challenges due to its persistence and the release of bisphenol A (BPA), a known xenoestrogen. Here, we report the isolation of <em>Bacillus subtilis</em> JNU01 (BsJNU01), capable of utilizing PC as its sole carbon source. Through transcriptomic analysis, we identified metallophosphoesterase from BsJNU01 (BsMPPE), the first reported metallophosphoesterase capable of degrading polycarbonate by catalyzing the hydrolysis of carbonate ester bonds. This enzyme operates under mild aqueous conditions (30 °C, pH 7), releasing 30 μmol of BPA as a monomer and demonstrating effective PC degradation under environmentally friendly conditions. PC biodegradation was confirmed by Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), and gas chromatography-mass spectrometry (GC-MS). Furthermore, surface and mechanical analyses revealed significant degradation and structural changes in PC films following BsMPPE treatment, with toughness showing a 40–70 % decrease compared to untreated PC films. This study represents a breakthrough in microbial plastic degradation, establishing a sustainable biocatalytic platform for PC recycling and upcycling technologies.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138330"},"PeriodicalIF":12.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143841718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-17DOI: 10.1016/j.jhazmat.2025.138309
Xing Wang , Juan Tong , Han Li , Mengjuan Lu , Yuan Liu , Hong Gan , Yifan Wang , Menglong Geng , Xuejiao Qie , Xiulong Wu , Hui Gao , Beibei Zhu , Shuman Tao , Xingyong Tao , Shuangqin Yan , Guopeng Gao , Xiaoyan Wu , Kun Huang , Yunxia Cao , Fangbiao Tao
Introduction
Exploring the stage-specific effects of prenatal exposure to organophosphate esters (OPEs) on offspring growth and developmental trajectories is critical for early-life health management.
Methods
Based on 2519 mother-child dyads from the Ma'anshan Birth Cohort, we examined the concentrations of OPEs in maternal urine during the three trimesters. Seventeen follow-up visits were made to the children, and physical data were collected. A grouped trajectory model was used to fit the growth trajectories.
Results
First-trimester bis(2-butoxyethyl) phosphate (BBOEP) was inversely associated with the children's adiposity rebound (AR) timing (β = −0.33, 95 % CI: −0.65, −0.01), and the ORs (95 % CIs) for early age at AR for each doubling of BBOEP and dibutyl phosphate (DBP) were 1.07 (1.00, 1.14) and 1.12 (1.03, 1.22), respectively. BBOEP increased the risk of a high-stable BMI-for-age z score (BMIz) group (OR = 1.18, 95 % CI: 1.01, 1.39), whereas tris(2-chloroethyl) phosphate (TCEP) and bis(2-ethylhexyl) phosphate reduced this risk. Diphenyl phosphate (OR = 0.74, 95 % CI: 0.59, 0.94) and aromatic OPEs (OR = 0.70, 95 % CI: 0.54, 0.90) reduced the odds of an extreme-high body fat group. TCEP also reduced the risk of a high body fat percentage group (p < 0.05). There appeared to be sex and ester bond differences in these associations, and breastfeeding could counteract the association between the OPEs and growth trajectories. No mixed effects of OPEs on BMIz trajectories were found.
Conclusions
The present study identified a heterogeneous association between OPE exposure during pregnancy and AR timing and physical growth patterns in offspring. Future studies are needed involving more regions and populations, with consideration of other developmentally toxic compounds, to obtain more reliable and comprehensive results.
{"title":"Sex-and stage-specific effect of prenatal exposure to organophosphate esters with children's physical growth patterns and adiposity rebound timing: Modification by breastfeeding","authors":"Xing Wang , Juan Tong , Han Li , Mengjuan Lu , Yuan Liu , Hong Gan , Yifan Wang , Menglong Geng , Xuejiao Qie , Xiulong Wu , Hui Gao , Beibei Zhu , Shuman Tao , Xingyong Tao , Shuangqin Yan , Guopeng Gao , Xiaoyan Wu , Kun Huang , Yunxia Cao , Fangbiao Tao","doi":"10.1016/j.jhazmat.2025.138309","DOIUrl":"10.1016/j.jhazmat.2025.138309","url":null,"abstract":"<div><h3>Introduction</h3><div>Exploring the stage-specific effects of prenatal exposure to organophosphate esters (OPEs) on offspring growth and developmental trajectories is critical for early-life health management.</div></div><div><h3>Methods</h3><div>Based on 2519 mother-child dyads from the Ma'anshan Birth Cohort, we examined the concentrations of OPEs in maternal urine during the three trimesters. Seventeen follow-up visits were made to the children, and physical data were collected. A grouped trajectory model was used to fit the growth trajectories.</div></div><div><h3>Results</h3><div>First-trimester bis(2-butoxyethyl) phosphate (BBOEP) was inversely associated with the children's adiposity rebound (AR) timing (<em>β</em> = −0.33, 95 % <em>CI</em>: −0.65, −0.01), and the <em>ORs</em> (95 % <em>CIs</em>) for early age at AR for each doubling of BBOEP and dibutyl phosphate (DBP) were 1.07 (1.00, 1.14) and 1.12 (1.03, 1.22), respectively. BBOEP increased the risk of a high-stable BMI-for-age z score (BMIz) group (<em>OR</em> = 1.18, 95 % <em>CI</em>: 1.01, 1.39), whereas tris(2-chloroethyl) phosphate (TCEP) and bis(2-ethylhexyl) phosphate reduced this risk. Diphenyl phosphate (<em>OR</em> = 0.74, 95 % <em>CI</em>: 0.59, 0.94) and aromatic OPEs (<em>OR</em> = 0.70, 95 % <em>CI</em>: 0.54, 0.90) reduced the odds of an extreme-high body fat group. TCEP also reduced the risk of a high body fat percentage group (<em>p</em> < 0.05). There appeared to be sex and ester bond differences in these associations, and breastfeeding could counteract the association between the OPEs and growth trajectories. No mixed effects of OPEs on BMIz trajectories were found.</div></div><div><h3>Conclusions</h3><div>The present study identified a heterogeneous association between OPE exposure during pregnancy and AR timing and physical growth patterns in offspring. Future studies are needed involving more regions and populations, with consideration of other developmentally toxic compounds, to obtain more reliable and comprehensive results.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138309"},"PeriodicalIF":12.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143841497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-17DOI: 10.1016/j.jhazmat.2025.138322
Mengyuan Xiao , Yongqing Yang , Hanin Alahmadi , Allison Harbolic , Gina M. Moreno , Terry Yu , Jerry Liu , Alex Guo , Genoa R. Warner , Phoebe A. Stapleton , Hao Chen
Microplastics (MPs) and nanoplastics (NPs) are pervasive pollutants and their analyses by traditional mass spectrometric methods require time-intensive sample preparation (e.g., extraction, digestion, and separation). This study presents a rapid and novel method for detecting MPs and NPs using flame ionization mass spectrometry (FI-MS) in which a dried sample (e.g., powder, soil and tissue) is directly burnt or heated with a flame in front of the MS inlet. FI-MS enables decomposition and ionization of various plastics such as polyethylene terephthalate (PET) and polystyrene (PS), allowing for analysis to be completed as fast as 10 seconds per sample. As a demonstration of application of this technique, PET contaminants in 1 L of bottled water or in 0.65 L of apple juice contained in plastic bottles were quickly detected from a filter paper after sample filtration and brief drying. A 0.89 mg soil sample spiked with 6000 ppm PET microplastics was measured to contain 4.98 µg of PET (5595 ppm, quantitation error: 6.8 %). Strikingly, PS nanoplastics (200 nm size) in mouse placentas were successfully identified and quantified, highlighting the method's ability to analyze biological tissue without tedious sample preparation. Overall, this study demonstrates the high potential of FI-MS for real-world sample analysis of MPs and NPs in environmental, biological, or consumer product samples.
{"title":"Rapid detection of microplastics and nanoplastics in seconds by mass spectrometry","authors":"Mengyuan Xiao , Yongqing Yang , Hanin Alahmadi , Allison Harbolic , Gina M. Moreno , Terry Yu , Jerry Liu , Alex Guo , Genoa R. Warner , Phoebe A. Stapleton , Hao Chen","doi":"10.1016/j.jhazmat.2025.138322","DOIUrl":"10.1016/j.jhazmat.2025.138322","url":null,"abstract":"<div><div>Microplastics (MPs) and nanoplastics (NPs) are pervasive pollutants and their analyses by traditional mass spectrometric methods require time-intensive sample preparation (e.g., extraction, digestion, and separation). This study presents a rapid and novel method for detecting MPs and NPs using flame ionization mass spectrometry (FI-MS) in which a dried sample (e.g., powder, soil and tissue) is directly burnt or heated with a flame in front of the MS inlet. FI-MS enables decomposition and ionization of various plastics such as polyethylene terephthalate (PET) and polystyrene (PS), allowing for analysis to be completed as fast as 10 seconds per sample. As a demonstration of application of this technique, PET contaminants in 1 L of bottled water or in 0.65 L of apple juice contained in plastic bottles were quickly detected from a filter paper after sample filtration and brief drying. A 0.89 mg soil sample spiked with 6000 ppm PET microplastics was measured to contain 4.98 µg of PET (5595 ppm, quantitation error: 6.8 %). Strikingly, PS nanoplastics (200 nm size) in mouse placentas were successfully identified and quantified, highlighting the method's ability to analyze biological tissue without tedious sample preparation. Overall, this study demonstrates the high potential of FI-MS for real-world sample analysis of MPs and NPs in environmental, biological, or consumer product samples.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138322"},"PeriodicalIF":12.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143841719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-17DOI: 10.1016/j.jhazmat.2025.138326
Tongyue Zhuo , Sibo Zhang , Zhong Zhang, Yanpeng Cai
The increasing presence of ibuprofen in aquatic ecosystems poses significant challenges to their biogeochemical functions, including nitrogen transformations. In this study, we employed 15N-labeling techniques to investigate the effects of environmentally relevant concentrations of ibuprofen (0–10,000 ng L−1) on denitrification and the associated nitrous oxide (N2O) emissions in river sediments over a 60-day period. The results revealed a hump-shaped response in denitrification rates to ibuprofen addition across a range of nitrate concentrations (1–60 mg N L−1), with rates peaking near 200 ng L−1 ibuprofen, followed by inhibition at certain higher concentrations, leading to a reduction of up to 25.8 % compared to the treatment without ibuprofen. Kinetic analysis showed that the maximum denitrification rate followed the same hump-shaped trend, despite a decrease in nitrate affinity with increasing ibuprofen concentrations. The abundance of denitrifying bacteria mirrored the pattern observed in denitrification rates across different ibuprofen concentrations. However, increasing ibuprofen concentrations consistently accelerated N2O production rates. Microbial analysis suggests that the increase in N2O production genes was faster than for reduction genes, while the decrease was slower with increasing ibuprofen concentrations. This study highlights the hump-shaped response of denitrification rates and the consistent increase in N2O emissions induced by ibuprofen, offering insights for developing environmental management strategies to mitigate ibuprofen and nitrogen pollution, as well as reducing N2O emissions.
布洛芬在水生生态系统中日益增加的存在对其生物地球化学功能（包括氮转化）构成了重大挑战。在这项研究中，我们采用15n标记技术研究了环境相关浓度的布洛芬（0-10,000 ng L-1）在60天内对河流沉积物中反硝化和相关氧化亚氮（N2O）排放的影响。结果显示，在硝酸盐浓度范围内（1-60 mg N - L-1），添加布洛芬对脱氮速率有驼峰状响应，在200 ng L-1布洛芬附近速率达到峰值，随后在某些较高浓度下受到抑制，与不添加布洛芬的处理相比，脱氮速率降低了25.8%。动力学分析表明，随着布洛芬浓度的增加，硝酸盐亲和力降低，但最大反硝化速率呈相同的驼峰型趋势。反硝化细菌的丰度反映了不同布洛芬浓度下反硝化率的变化规律。然而，增加布洛芬浓度持续加速N2O的生成速率。微生物分析表明，N2O产生基因的增加速度快于还原基因，而随着布洛芬浓度的增加，N2O产生基因的减少速度较慢。该研究强调了布洛芬引起的反硝化速率的驼峰响应和N2O排放的持续增加，为制定环境管理策略以减轻布洛芬和氮污染以及减少N2O排放提供了见解。
{"title":"Differential effects of environmentally relevant concentrations of ibuprofen on denitrification and nitrous oxide emissions in river sediments","authors":"Tongyue Zhuo , Sibo Zhang , Zhong Zhang, Yanpeng Cai","doi":"10.1016/j.jhazmat.2025.138326","DOIUrl":"10.1016/j.jhazmat.2025.138326","url":null,"abstract":"<div><div>The increasing presence of ibuprofen in aquatic ecosystems poses significant challenges to their biogeochemical functions, including nitrogen transformations. In this study, we employed <sup>15</sup>N-labeling techniques to investigate the effects of environmentally relevant concentrations of ibuprofen (0–10,000 ng L<sup>−1</sup>) on denitrification and the associated nitrous oxide (N<sub>2</sub>O) emissions in river sediments over a 60-day period. The results revealed a hump-shaped response in denitrification rates to ibuprofen addition across a range of nitrate concentrations (1–60 mg N L<sup>−1</sup>), with rates peaking near 200 ng L<sup>−1</sup> ibuprofen, followed by inhibition at certain higher concentrations, leading to a reduction of up to 25.8 % compared to the treatment without ibuprofen. Kinetic analysis showed that the maximum denitrification rate followed the same hump-shaped trend, despite a decrease in nitrate affinity with increasing ibuprofen concentrations. The abundance of denitrifying bacteria mirrored the pattern observed in denitrification rates across different ibuprofen concentrations. However, increasing ibuprofen concentrations consistently accelerated N<sub>2</sub>O production rates. Microbial analysis suggests that the increase in N<sub>2</sub>O production genes was faster than for reduction genes, while the decrease was slower with increasing ibuprofen concentrations. This study highlights the hump-shaped response of denitrification rates and the consistent increase in N<sub>2</sub>O emissions induced by ibuprofen, offering insights for developing environmental management strategies to mitigate ibuprofen and nitrogen pollution, as well as reducing N<sub>2</sub>O emissions.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138326"},"PeriodicalIF":12.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-17DOI: 10.1016/j.jhazmat.2025.138317
Julio Fernández-Arribas, Teresa Moreno, Ethel Eljarrat
A total diet study focused on exposure to plastic additives has been performed on 109 food samples. Plasticizers were detected in 85 % of analyzed samples with total concentrations ranging between not detected (nd) and 22.0 µg/g wet weight (ww). Non-phthalate plasticizers (NPPs), such as acetyl tributyl citrate (ATBC) or di(2-ethylhexyl) adipate (DEHA), were detected predominantly in baby foods (nd-3.38 µg/g ww) and meat (nd-15.0 µg/g ww), respectively. Significant differences (p ≤ 0.001) were observed across foods with different packaging types regarding the presence of ATBC and DEHA. ATBC was primarily detected in foods packaged in glass containers, meanwhile DEHA is mainly related to fresh food wrapped in plastic materials. Additionally, transference assays in selected ready to cook meals and fresh vegetables were performed, with NPPs exhibiting a higher transference from packaging to food than other compounds. The data obtained have been used for an assessment of estimated daily intake (EDI) of plastic additives in infants (6–12 months), toddlers (1–3 years), and adults (>18 years), resulting in values ranging 0.29–516 µg/kg body weight (bw)/day. Human risk related to baby food consumption, expressed as hazard quotients (HQs), was found with di(2-ethylhexyl) phthalate (DEHP) in the infant population sub-group.
{"title":"Plastic additives in the diet: Occurrence and dietary exposure in different population groups","authors":"Julio Fernández-Arribas, Teresa Moreno, Ethel Eljarrat","doi":"10.1016/j.jhazmat.2025.138317","DOIUrl":"10.1016/j.jhazmat.2025.138317","url":null,"abstract":"<div><div>A total diet study focused on exposure to plastic additives has been performed on 109 food samples. Plasticizers were detected in 85 % of analyzed samples with total concentrations ranging between not detected (nd) and 22.0 µg/g wet weight (ww). Non-phthalate plasticizers (NPPs), such as acetyl tributyl citrate (ATBC) or di(2-ethylhexyl) adipate (DEHA), were detected predominantly in baby foods (nd-3.38 µg/g ww) and meat (nd-15.0 µg/g ww), respectively. Significant differences (p ≤ 0.001) were observed across foods with different packaging types regarding the presence of ATBC and DEHA. ATBC was primarily detected in foods packaged in glass containers, meanwhile DEHA is mainly related to fresh food wrapped in plastic materials. Additionally, transference assays in selected ready to cook meals and fresh vegetables were performed, with NPPs exhibiting a higher transference from packaging to food than other compounds. The data obtained have been used for an assessment of estimated daily intake (EDI) of plastic additives in infants (6–12 months), toddlers (1–3 years), and adults (>18 years), resulting in values ranging 0.29–516 µg/kg body weight (bw)/day. Human risk related to baby food consumption, expressed as hazard quotients (HQs), was found with di(2-ethylhexyl) phthalate (DEHP) in the infant population sub-group.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138317"},"PeriodicalIF":12.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143841498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-17DOI: 10.1016/j.jhazmat.2025.138333
Yating Lu , Fuqing He , Xu Zhu , Jing Tang , Yue Lu , Jiajia Wang , Jiangfang Yu , Li Chen , Xingyang Cheng , Tianhao Liu , Lin Tang
Rapid, on-site accurate tracking of harmful mycotoxin in food waste composting is essential to provide instant content information for efficient supervision. However, on-site analytical tools especially colorimetric sensors currently suffer from low sensitivity/selectivity and poor environment robustness, posing hurdles for their applications. In this work, we have proposed a portable paper-based colorimetric sensor to detect the representative mycotoxin (aflatoxin B1, AFB1) in the composting process, where the superior catalytic velocity (6-fold higher than the natural enzyme) and well-regulated catalytic activity of nanozyme by the aptamers ensure the high sensitivity (a wide linear range: 0.1–1000 ng/mL; an ultra-low limit of detection: 0.082 ng/mL) and good selectivity of the colorimetric sensing, respectively. The smartphone-based platform exhibits high accuracy with the relative standard deviation within 3.67 % compared commercial enzyme-linked immunosorbent assay. Finally, on-site tracking of the AFB1 content in food waste during the composting process with or without the oxidant (potassium persulfate) has been carried out using the developed colorimetric sensor. It is concluded that reducing the AFB1 generation in the food waste is more meaningful than the compost treatment. This study offers a promising method for in situ analysis of trace AFB1 in food waste compost to ensure environmental and human health safety.
{"title":"On-site tracking of trace Aflatoxin B1 in food waste composting via a portable colorimetric sensing platform with nanozymes","authors":"Yating Lu , Fuqing He , Xu Zhu , Jing Tang , Yue Lu , Jiajia Wang , Jiangfang Yu , Li Chen , Xingyang Cheng , Tianhao Liu , Lin Tang","doi":"10.1016/j.jhazmat.2025.138333","DOIUrl":"10.1016/j.jhazmat.2025.138333","url":null,"abstract":"<div><div>Rapid, on-site accurate tracking of harmful mycotoxin in food waste composting is essential to provide instant content information for efficient supervision. However, on-site analytical tools especially colorimetric sensors currently suffer from low sensitivity/selectivity and poor environment robustness, posing hurdles for their applications. In this work, we have proposed a portable paper-based colorimetric sensor to detect the representative mycotoxin (aflatoxin B1, AFB1) in the composting process, where the superior catalytic velocity (6-fold higher than the natural enzyme) and well-regulated catalytic activity of nanozyme by the aptamers ensure the high sensitivity (a wide linear range: 0.1–1000 ng/mL; an ultra-low limit of detection: 0.082 ng/mL) and good selectivity of the colorimetric sensing, respectively. The smartphone-based platform exhibits high accuracy with the relative standard deviation within 3.67 % compared commercial enzyme-linked immunosorbent assay. Finally, on-site tracking of the AFB1 content in food waste during the composting process with or without the oxidant (potassium persulfate) has been carried out using the developed colorimetric sensor. It is concluded that reducing the AFB1 generation in the food waste is more meaningful than the compost treatment. This study offers a promising method for in situ analysis of trace AFB1 in food waste compost to ensure environmental and human health safety.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138333"},"PeriodicalIF":12.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143841499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-17DOI: 10.1016/j.jhazmat.2025.138331
Wenshuai Ye , Ning Liu , Sicheng Li , Guo Zhao , Gang Liu
The cost-effective, disposable, batch-prepared electrochemical sensors with high sensitivity and accurate detection are essential to the rapid on-site detection of heavy metals in soil and water. Here, we report a one-step laser additive manufacturing technique for the batch fabrication of electrochemical sensors. With the systematic optimization of multiple laser parameters (including laser power, engraving speed, and frequency), highly sensitive laser-induced porous graphene (LIPG) electrodes were fabricated to detect Cd2+ and Pb2+ efficiently. Followingly, with the modification of Nafion and bismuth-film, the obtained Bi/Nafion/LIPG electrode showed excellent repeatability, stability, selectivity, and outstanding detection limits of 0.25 µg/L for Cd2+ and 0.41 µg/L for Pb2+, respectively. As-prepared Bi/Nafion/LIPG electrode also works in actual soil and water samples, with an average recovery of 99.61 and 99.90 for Cd2+ and Pb2+. Furthermore, from the perspective of the laser photothermal effect, the important influence of laser parameters on the physical and chemical properties of LIPG was analyzed, confirming that the laser photothermal effect has a superimposed cumulative effect on the surface of the PI film. This study provides a new methodical guidance for the rapid and batch preparation of LIPG electrodes with the advantages of low-cost, disposable, high-sensitivity, and flexibility, which can promote the on-site electrochemical detection of heavy metals in soil and water.
{"title":"Methodological guidance to the batch preparation of graphene-based sensor for low-cost and high-performance Cd2+ and Pb2+ detection","authors":"Wenshuai Ye , Ning Liu , Sicheng Li , Guo Zhao , Gang Liu","doi":"10.1016/j.jhazmat.2025.138331","DOIUrl":"10.1016/j.jhazmat.2025.138331","url":null,"abstract":"<div><div>The cost-effective, disposable, batch-prepared electrochemical sensors with high sensitivity and accurate detection are essential to the rapid on-site detection of heavy metals in soil and water. Here, we report a one-step laser additive manufacturing technique for the batch fabrication of electrochemical sensors. With the systematic optimization of multiple laser parameters (including laser power, engraving speed, and frequency), highly sensitive laser-induced porous graphene (LIPG) electrodes were fabricated to detect Cd<sup>2+</sup> and Pb<sup>2+</sup> efficiently. Followingly, with the modification of Nafion and bismuth-film, the obtained Bi/Nafion/LIPG electrode showed excellent repeatability, stability, selectivity, and outstanding detection limits of 0.25 µg/L for Cd<sup>2+</sup> and 0.41 µg/L for Pb<sup>2+</sup>, respectively. As-prepared Bi/Nafion/LIPG electrode also works in actual soil and water samples, with an average recovery of 99.61 and 99.90 for Cd<sup>2+</sup> and Pb<sup>2+</sup>. Furthermore, from the perspective of the laser photothermal effect, the important influence of laser parameters on the physical and chemical properties of LIPG was analyzed, confirming that the laser photothermal effect has a superimposed cumulative effect on the surface of the PI film. This study provides a new methodical guidance for the rapid and batch preparation of LIPG electrodes with the advantages of low-cost, disposable, high-sensitivity, and flexibility, which can promote the on-site electrochemical detection of heavy metals in soil and water.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138331"},"PeriodicalIF":12.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143841500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-17DOI: 10.1016/j.jhazmat.2025.138319
Bin Wang , Ruiqiu Fang , Guwen Zhang , Na Liu , Zhijuan Feng , Yuanpeng Bu , Yaming Gong
Cadmium (Cd) toxicity poses a severe environmental threat, leading to substantial declines in crop growth and productivity worldwide. Accumulation of proline is recognized as a critical adaptive response in plants facing heavy metal stress. However, the precise mechanisms regulating Cd-induced proline accumulation remain elusive. Here, we discovered that a Cd-induced CCT5 gene in vegetable soybean, designated as GmCIC5, plays a pivotal role in regulating proline biosynthesis by activating the transcription of the proline biosynthesis gene, GmP5CS. Under greenhouse conditions, silencing GmCIC5 plants exhibited heightened sensitivity to Cd stress and suppressed root growth after 15 days of 30 mg/L CdCl2 treatment. Furthermore, qRT-PCR and ChIP-qPCR revealed that GmCIC5 directly targeted GmP5CS. Consequently, the GmCIC5 knockdown plants showed drastically reduced levels of GmP5CS transcripts and proline accumulation. Overexpression of GmP5CS largely suppressed the Cd hypersensitivity phenotype of GmCIC5 knockdown plants, suggesting that GmCIC5-mediated Cd tolerance was dependent on proline. Taken together, our results indicated that GmCIC5 is a novel regulator of Cd stress tolerance through proline biosynthesis, which may provide new insights into the molecular mechanisms of plant responses to Cd stress and offer a theoretical foundation for breeding Cd-resistant crops.
{"title":"A CCT protein GmCIC5 activates GmP5CS to regulate proline accumulation and cadmium tolerance in vegetable soybean","authors":"Bin Wang , Ruiqiu Fang , Guwen Zhang , Na Liu , Zhijuan Feng , Yuanpeng Bu , Yaming Gong","doi":"10.1016/j.jhazmat.2025.138319","DOIUrl":"10.1016/j.jhazmat.2025.138319","url":null,"abstract":"<div><div>Cadmium (Cd) toxicity poses a severe environmental threat, leading to substantial declines in crop growth and productivity worldwide. Accumulation of proline is recognized as a critical adaptive response in plants facing heavy metal stress. However, the precise mechanisms regulating Cd-induced proline accumulation remain elusive. Here, we discovered that a Cd-induced <em>CCT5</em> gene in vegetable soybean, designated as <em>GmCIC5</em>, plays a pivotal role in regulating proline biosynthesis by activating the transcription of the proline biosynthesis gene, <em>GmP5CS</em>. Under greenhouse conditions, silencing <em>GmCIC5</em> plants exhibited heightened sensitivity to Cd stress and suppressed root growth after 15 days of 30 mg/L CdCl<sub>2</sub> treatment. Furthermore, qRT-PCR and ChIP-qPCR revealed that GmCIC5 directly targeted <em>GmP5CS</em>. Consequently, the <em>GmCIC5</em> knockdown plants showed drastically reduced levels of <em>GmP5CS</em> transcripts and proline accumulation. Overexpression of <em>GmP5CS</em> largely suppressed the Cd hypersensitivity phenotype of <em>GmCIC5</em> knockdown plants, suggesting that GmCIC5-mediated Cd tolerance was dependent on proline. Taken together, our results indicated that GmCIC5 is a novel regulator of Cd stress tolerance through proline biosynthesis, which may provide new insights into the molecular mechanisms of plant responses to Cd stress and offer a theoretical foundation for breeding Cd-resistant crops.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138319"},"PeriodicalIF":12.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143841716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-17DOI: 10.1016/j.jhazmat.2025.138325
Lin Zhang , Helin Wang , Xiaohan Liu , Hongbin Zhan , Uwe Schneidewind , Stefan Krause , Menggui Jin , Xing Liang , Yanfeng Liu , Ping Li
Agricultural soils in riparian zones near rivers often experience frequent water table fluctuations, which can lead to increased nitrogen losses and greenhouse gas emissions via the nitrogen biogeochemical processes. However, the influence of water table fluctuations on the multiple nitrogen transformation processes that dominate nitrate attenuation and nitrous oxide (N2O) effluxes remains poorly understood. In this study, the dynamic changes in depth-dependent nitrate attenuation and soil N2O effluxes, and the responses of microbial communities influenced by water table fluctuations were studied using a series of large column experiments. Our results revealed that dissolved oxygen (DO) concentrations at a depth of −10 cm in sand columns with three different grain sizes (fine→medium→coarse) oscillated, producing oxidizing conditions during drainage and reducing conditions during imbibition periods. DO micro-sensors installed in a layered (sand and sandy loam) column as well as in two sandy loam columns with different regimes in induced water table changes all revealed steady hypoxic conditions. The diversity of the microbial community was significantly correlated with total nitrogen, total organic carbon, and nitrate concentrations, as well as potential denitrification rates. The dominant microbial populations related to the nrfA gene were Methanothrix and Sedimentibacte, whereas those related to denitrification (nirK, nirS, and nosZ) were Pseudomonas and Sulfuricaulis. These findings improve our understanding of the effects of water table fluctuations on groundwater nitrate loss in riparian corridors.
{"title":"Denitrification dominates nitrate attenuation and nitrous oxide effluxes under water table fluctuations","authors":"Lin Zhang , Helin Wang , Xiaohan Liu , Hongbin Zhan , Uwe Schneidewind , Stefan Krause , Menggui Jin , Xing Liang , Yanfeng Liu , Ping Li","doi":"10.1016/j.jhazmat.2025.138325","DOIUrl":"10.1016/j.jhazmat.2025.138325","url":null,"abstract":"<div><div>Agricultural soils in riparian zones near rivers often experience frequent water table fluctuations, which can lead to increased nitrogen losses and greenhouse gas emissions via the nitrogen biogeochemical processes. However, the influence of water table fluctuations on the multiple nitrogen transformation processes that dominate nitrate attenuation and nitrous oxide (N<sub>2</sub>O) effluxes remains poorly understood. In this study, the dynamic changes in depth-dependent nitrate attenuation and soil N<sub>2</sub>O effluxes, and the responses of microbial communities influenced by water table fluctuations were studied using a series of large column experiments. Our results revealed that dissolved oxygen (DO) concentrations at a depth of −10 cm in sand columns with three different grain sizes (fine→medium→coarse) oscillated, producing oxidizing conditions during drainage and reducing conditions during imbibition periods. DO micro-sensors installed in a layered (sand and sandy loam) column as well as in two sandy loam columns with different regimes in induced water table changes all revealed steady hypoxic conditions. The diversity of the microbial community was significantly correlated with total nitrogen, total organic carbon, and nitrate concentrations, as well as potential denitrification rates. The dominant microbial populations related to the <em>nrfA</em> gene were <em>Methanothrix</em> and <em>Sedimentibacte</em>, whereas those related to denitrification (<em>nirK</em>, <em>nirS</em>, and <em>nosZ</em>) were <em>Pseudomonas</em> and <em>Sulfuricaulis</em>. These findings improve our understanding of the effects of water table fluctuations on groundwater nitrate loss in riparian corridors.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138325"},"PeriodicalIF":12.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143841717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-17DOI: 10.1016/j.jhazmat.2025.138332
Linjun Qin , Wuyuxin Zhu , Lili Yang , Minghui Zheng , Guorui Liu
Environmentally persistent free radicals (EPFRs) are environmental pollutants whose potential DNA damage and apoptosis toxicity may be mediated by reactive oxygen species (ROS). The currently available knowledge of their environmental characteristics and transformation mechanisms is not sufficient to understand the environmental behaviors and health effects of EPFRs and should be further expanded. This review offers a comprehensive review of the current state of EPFRs, including characterization methods, formation mechanisms, and environmental behavior of EPFRs. Electron paramagnetic resonance (EPR) spectroscopy directly probes EPFRs in environmental matrices. EPFRs can be simply categorized by g value, but structure confirmation solely by EPR is challenging because the complexity of environmental matrices results in the absence of a hyperfine splitting spectrum. Combined advanced EPR and multi-spectroscopic methods enable the structural identification of EPFRs in environmental samples. The environmental behavior and ecological impacts of EPFRs have been progressively studied. This review highlights the important role of EPFRs in natural environments and emphasizes the necessity of further research on EPFRs.
{"title":"Persistent free radicals in the environment","authors":"Linjun Qin , Wuyuxin Zhu , Lili Yang , Minghui Zheng , Guorui Liu","doi":"10.1016/j.jhazmat.2025.138332","DOIUrl":"10.1016/j.jhazmat.2025.138332","url":null,"abstract":"<div><div>Environmentally persistent free radicals (EPFRs) are environmental pollutants whose potential DNA damage and apoptosis toxicity may be mediated by reactive oxygen species (ROS). The currently available knowledge of their environmental characteristics and transformation mechanisms is not sufficient to understand the environmental behaviors and health effects of EPFRs and should be further expanded. This review offers a comprehensive review of the current state of EPFRs, including characterization methods, formation mechanisms, and environmental behavior of EPFRs. Electron paramagnetic resonance (EPR) spectroscopy directly probes EPFRs in environmental matrices. EPFRs can be simply categorized by g value, but structure confirmation solely by EPR is challenging because the complexity of environmental matrices results in the absence of a hyperfine splitting spectrum. Combined advanced EPR and multi-spectroscopic methods enable the structural identification of EPFRs in environmental samples. The environmental behavior and ecological impacts of EPFRs have been progressively studied. This review highlights the important role of EPFRs in natural environments and emphasizes the necessity of further research on EPFRs.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138332"},"PeriodicalIF":12.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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