Journal of Hazardous Materials最新文献_第10页

Microplastic contamination in plants: Seed-to-harvest exposure and quantified evidence from medicinal crops 植物中的微塑料污染：药用作物从种子到收获的暴露和量化证据

IF 11.3 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials

Pub Date : 2026-01-10 DOI: 10.1016/j.jhazmat.2026.141105

Tongshan Zhu , Jingjing Yin , Yang Yu , Li Yao , Xiaozun Li , Yongqing Zhang , Guoxin Xu , Fayuan Wang

Soil-retained microplastics (MPs) are in direct contact with plant roots. However, their adhesion, penetration, and potential transport in crops at different developmental stages, particularly the contamination of roots following long-term exposure, remain poorly understood. In this study, two widely cultivated medicinal/root crops, Danshen (Salvia miltiorrhiza) and milkvetch (Astragalus membranaceus), were exposed to various types of MPs (0.5–5 μm in size), including polystyrene, polyethylene, polyvinyl chloride, and polylactic acid. Using a novel quantitative approach, we characterized the dynamics of MPs adsorption and penetration from germination to harvest. Results indicate that seed imbibition attracts MPs from surrounding soil, creating hot-spots and disrupting redox homeostasis in seeds. The penetration of MPs into seedling roots is species- and biostructure- dependent, while their distribution in mature roots shows similarities. Specifically, in seedling roots of Danshen, MPs are blocked by the exodermis, whereas in milkvetch, MPs enter the cortex. The periderm of mature roots acts as a double-edged sword: it prevents MPs from entering the vascular system and potential root-shoot transport, while trapping approximately 140,000–150,000 particles per gram of 1 μm MPs after 120 days of exposure at 50 mg/kg. Through the distinctive lens of biological microstructure, our study reveals the mechanisms of MPs-plant interaction throughout the vegetative growth phase, specifically quantifying MPs' contamination levels and their restricted distribution within the outermost barrier tissues. These findings establish a scientific foundation for optimizing cultivation practices and processing methods to reduce MPs in plant-based products.

土壤保留的微塑料（MPs）与植物根部直接接触。然而，它们在作物不同发育阶段的粘附、渗透和潜在运输，特别是长期暴露后对根系的污染，仍然知之甚少。本研究将两种广泛种植的药用/根茎作物丹参（丹参）和黄芪（黄芪）暴露于不同类型的MPs（大小为0.5 ~ 5 μm）中，包括聚苯乙烯、聚乙烯、聚氯乙烯和聚乳酸。利用一种新的定量方法，我们表征了MPs从萌发到收获的吸附和渗透动力学。结果表明，种子吸胀会吸引周围土壤中的MPs，形成热点并破坏种子的氧化还原稳态。MPs在幼苗根系中的渗透是物种和生物结构依赖的，而它们在成熟根系中的分布是相似的。具体来说，在丹参幼苗根中，MPs被外表皮阻断，而在黄芪中，MPs进入皮层。成熟根周起着双刃剑的作用：它阻止MPs进入维管系统和潜在的根尖运输，同时在50 mg/kg的浓度下暴露120天后，每克1 μm MPs捕获约14万至15万个颗粒。通过独特的生物微观结构，我们的研究揭示了整个营养生长阶段MPs与植物相互作用的机制，特别是量化MPs的污染水平及其在最外层屏障组织中的限制分布。这些发现为优化栽培方法和加工方法以减少植物性产品中的MPs奠定了科学基础。

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引用次数: 0

Single-atom Cu nanozyme anchored on carbon nitride with enhanced peroxidase-like activity for colorimetric detection of foodborne pathogens 固结在氮化碳上的具有增强过氧化物酶样活性的单原子铜纳米酶用于食源性病原体的比色检测

IF 11.3 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials

Pub Date : 2026-01-10 DOI: 10.1016/j.jhazmat.2026.141108

Fengyu Guo , Hua Lei , Qian Yang , Nana Jin , Xinyang Zhang , Jianhan Lin , Ruibing Hu , Baocheng Zhou , Wen Wang , Hua Yang , Xiaoping Dong

Nanozyme-based colorimetric biosensors for foodborne pathogens are promising, but their sensitivity is often limited by the insufficient catalytic performance of nanozyme probes. Herein, a copper single-atom nanozyme anchored on carbon nitride (Cu-SA-CN), with a Cu loading of 24.1 wt%, was fabricated via a novel supramolecular self-assembly and pyrolysis strategy. The material features atomically dispersed Cu-N₃ sites and exhibits superior peroxidase-like activity (V_max = 51.4 ×10⁻⁸ M s⁻¹) by efficiently catalyzing H₂O₂ to generate reactive oxygen species (ROS). Leveraging the abundant surface amino groups of Cu-SA-CN for antibody conjugation and its high activity for signal amplification, a sensitive immunomagnetic colorimetric biosensor was constructed for Salmonella typhimurium (S. typhimurium) detection, where magnetic beads enable target enrichment and Cu-SA-CN serves as a robust signal amplifier. This biosensor achieved a low detection limit of 12.7 CFU mL⁻¹ with a linear range from 3.8 × 10¹ – 3.8 × 10⁶ CFU mL⁻¹. Importantly, the sensor exhibited excellent long-term stability. Successful application in spiked chicken samples highlighted its practicality. This work not only provides an effective strategy for creating high-performance single-atom nanozymes but also offers a new approach for the sensitive and specific detection of pathogenic bacteria in complex food matrices.

基于纳米酶的食源性病原体比色生物传感器很有前景，但其灵敏度往往受到纳米酶探针催化性能不足的限制。本文通过超分子自组装和热解方法制备了一种锚定在氮化碳上的铜单原子纳米酶（Cu- sa - cn）， Cu负载为24.1% wt%。该材料具有原子分散的Cu-N3位点，通过有效催化H2O2生成活性氧（ROS），表现出优异的过氧化物酶样活性（Vmax = 51.4 ×10−8 M s−1）。利用Cu-SA-CN丰富的表面氨基可偶联抗体和其高活性的信号放大，构建了一种用于鼠伤寒沙门氏菌检测的灵敏免疫磁比色生物传感器，其中磁珠可实现靶富集，Cu-SA-CN可作为鲁棒信号放大器。该生物传感器的最低检测限为12.7 CFU mL - 1，线性范围为3.8 × 101 - 3.8 × 106 CFU mL - 1。重要的是，该传感器表现出优异的长期稳定性。在加标鸡肉样品中的成功应用突出了该方法的实用性。这项工作不仅为构建高性能的单原子纳米酶提供了有效的策略，而且为复杂食品基质中致病菌的敏感和特异性检测提供了新的方法。

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引用次数: 0

Occurrence and prioritization of hydrophobic pollutants in China’s coastal marine biota 中国沿海海洋生物群中疏水污染物的发生及优先级

IF 11.3 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials

Pub Date : 2026-01-10 DOI: 10.1016/j.jhazmat.2026.141110

Haichao Mu , Ye Sun , Xiaodan Pei , Xuchun Li , Jiajia Wu , Wenwen Wang , Rong Cao , Xing Liu , Ziwei Yao

Pollutants in the marine environment directly affect biota and can be transmitted to humans through the food chain. However, comprehensive biomonitoring data for coastal marine biota remain limited. In this study, a suspect screening analysis (SSA) workflow using gas chromatography quadrupole time-of-flight mass spectrometry (GC-QTOF/MS) was conducted to investigate 830 hydrophobic pollutants in 130 marine biota samples collected from four major coastal seas in China (the Bohai, Yellow, East China, and South China Seas). A total of 165 hydrophobic pollutants were identified, with 40 high-frequency compounds across eight groups. Plasticizers were present at the highest concentrations, followed by ketones and polycyclic aromatic hydrocarbons (PAHs). The identified pollutants exhibited regional variations across the four seas, with higher pollution levels in the southern seas compared to the northern ones. Based on the Toxicological Priority Index framework, dibutyl phthalate was identified as the compound with the highest toxicity. The hazard quotient values for 28 compounds exceeded 1, with 4-Methylphenol ranking first, indicating potential risks from emerging chemicals in coastal marine biota. These findings provide comprehensive data for monitoring hydrophobic pollutants in coastal biota across China and highlight the need for expanded biomonitoring of emerging chemicals of concern in marine ecosystems.

海洋环境中的污染物直接影响生物群，并可通过食物链传播给人类。然而，沿海海洋生物群的综合生物监测数据仍然有限。本研究采用气相色谱-四极杆飞行时间质谱（GC-QTOF/MS）对中国4个主要沿海海域（渤海、黄海、东海和南海）的130个海洋生物群样品中的830种疏水污染物进行了可疑筛选分析（SSA）。共鉴定出165种疏水污染物，其中40种高频化合物分布在8组中。增塑剂的浓度最高，其次是酮类和多环芳烃（PAHs）。所确定的污染物在四个海域之间表现出区域差异，南部海域的污染水平高于北部海域。根据毒理学优先指数框架，邻苯二甲酸二丁酯被确定为毒性最高的化合物。28种化合物的危害商值超过1，其中4-甲基苯酚位居首位，表明新兴化学物质对沿海海洋生物群存在潜在风险。这些发现为监测中国沿海生物群中的疏水污染物提供了全面的数据，并强调了扩大对海洋生态系统中关注的新兴化学物质的生物监测的必要性。

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引用次数: 0

Hidden Pollutants in Food: Evidence of Small Microplastic Particles (100–5 µm) in Refined Sugar from the Italian Market 食品中隐藏的污染物：来自意大利市场的精制糖中含有小微塑料颗粒（100-5微米）的证据

IF 13.6 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials

Pub Date : 2026-01-10 DOI: 10.1016/j.jhazmat.2026.141113

Marta Fadda, Alessio Sacco, Andrea Mario Rossi, Andrea Mario Giovannozzi

Microplastics (MPs) are emerging contaminants increasingly reported in food, including sugar. This study presents a novel analytical approach enabling the detection of MPs < 20 µm without chemical digestion, preserving particle integrity through simple dissolution in ultrapure water. White sugars from major Italian brands and distributors were analyzed using µ-FTIR for MPs ≥ 50 µm and µ-Raman for particles between 5-50 µm. The µ-Raman results are semi-quantitative and based on sub-sampling of 34% of the filter area. While µ-FTIR detected relatively few MPs, µ-Raman revealed that the majority of particles fell below 20 µm, with a strong predominance in the 5-10 µm range. PVC emerged as the most abundant polymer, followed by PE, PS, PP and PET. The presence of MPs in a widely consumed product raises questions regarding food safety and human exposure. The predominance of MPs < 20 µm - a fraction not fully addressed by current EU regulatory frameworks - underscores the need for harmonized, standardized methodologies for monitoring MPs in food.

微塑料（MPs）是包括糖在内的食品中越来越多的新兴污染物。本研究提出了一种新的分析方法，可以在没有化学消化的情况下检测MPs <； 20µm，通过在超纯水中简单溶解保持颗粒完整性。对来自意大利主要品牌和经销商的白糖进行分析，对MPs≥50µm的使用µ-FTIR，对5-50µm的使用µ-拉曼。微拉曼结果是半定量的，基于过滤面积的34%的次采样。虽然μ -FTIR检测到的MPs相对较少，但μ -Raman显示，大多数颗粒在20 μ m以下，在5-10 μ m范围内具有很强的优势。聚氯乙烯成为最丰富的聚合物，其次是PE、PS、PP和PET。在广泛消费的产品中存在MPs引发了有关食品安全和人类暴露的问题。目前欧盟监管框架尚未完全解决的20 μ m的MPs占主导地位的问题，强调了对食品中MPs监测的统一、标准化方法的必要性。

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引用次数: 0

Seasonal dynamics and stability of gut antibiotic resistance genes in plateau pika (Ochotona curzoniae) and plateau zokor (Eospalax baileyi) 高原鼠兔和高原鼢鼠肠道抗生素耐药基因的季节性动态及稳定性

IF 11.3 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials

Pub Date : 2026-01-10 DOI: 10.1016/j.jhazmat.2026.141112

Yu Wang , Yuan Qian , Chenwei Shi , Jie Bi , Pengcheng Dong , Yongqiang Zou , Yayuan Yang , Yuanqing Tao , Huan Li

The extreme ecosystems of the Tibetan Plateau harbor an alarming reservoir of antibiotic resistance genes (ARGs) within wildlife gut microbiomes—an emerging contaminant with significant implications for One Health. However, seasonal dynamics and pathogenic risks of these ARGs remain poorly understood. Through a 2.5-year study comparing the subterranean plateau zokor (Eospalax baileyi) and the surface-dwelling plateau pika (Ochotona curzoniae), we demonstrate how species-specific ecological strategies shape distinct ARG transmission patterns. Our results demonstrated striking eco-evolutionary trade-offs: surface pikas showed 1.3 times higher ARG diversity with strong seasonal variation, influenced by temperature-modulated bacterial community turnover and mobile genetic elements (MGEs). Conversely, subterranean zokors maintained stable, were enriched with high-risk ARGs dominated by mepA and tetO, reflecting their isolated niche. Both species consistently carried pathogenic-ARG complexes (Clostridium-bacA, Staphylococcus-Ermb) across seasons, genders, and ages, indicating established resistance reservoirs in plateau food webs. Metagenomic binning revealed co-transfer potential between ARGs and MGEs. This pioneering longitudinal study exposes a dual pattern: seasonal changes cause temporary turnover of ARGs, which harbor lower resistance risk in surface-dwelling animals, while subterranean hosts retain stable, higher-risk resistance. These findings establish critical baselines for monitoring antimicrobial resistance in vulnerable ecosystems and underscore the need for integrated One Health strategies.

在青藏高原的极端生态系统中，野生动物肠道微生物群中存在着惊人的抗生素耐药基因（ARGs），这是一种对“同一个健康”有重大影响的新兴污染物。然而，这些ARGs的季节性动态和致病风险仍然知之甚少。通过对地下高原鼢鼠（Eospalax baileyi）和地表高原鼠兔（Ochotona curzoniae）为期2.5年的研究，我们展示了物种特异性生态策略如何塑造不同的ARG传播模式。我们的研究结果显示了惊人的生态进化权衡：受温度调节细菌群落更替和移动遗传元件（MGEs）的影响，地表鼠兔的ARG多样性高1.3倍，具有强烈的季节变化。相反，地下动物保持稳定，富含以mepA和tetO为主的高风险ARGs，反映了其孤立的生态位。这两个物种在不同季节、性别和年龄都一致携带致病性arg复合物（双歧杆菌、葡萄球菌），表明在高原食物网中建立了抗性储存库。宏基因组分析揭示了ARGs和MGEs之间的共转移潜力。这项开创性的纵向研究揭示了一种双重模式：季节变化导致ARGs的暂时更替，这在地表动物中具有较低的抗性风险，而地下宿主则保持稳定，高风险的抗性。这些发现为监测脆弱生态系统中抗菌素耐药性确立了关键基线，并强调了综合“同一个健康”战略的必要性。

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引用次数: 0

Regulation of reactive species for the mitigation of biotoxicity in bio-treated effluent from electrochemically oxidized landfill leachate 调节活性物种以减轻电化学氧化垃圾渗滤液生物处理出水的生物毒性

IF 11.3 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials

Pub Date : 2026-01-10 DOI: 10.1016/j.jhazmat.2026.141107

Mandi Liu , Pengyu Liang , Xiaofan Liu , Kainan Zhang , Shuchi Zhang , Donglei Wu

Landfill leachate bio-treated effluent (LLBE) is characterized by high levels of chloride ions and complex organic pollutants with poor biodegradability. The use of electrochemical oxidation (EO) to the water sample can enhance its biodegradability. However, the generation of chlorinated disinfection by-products (Cl-DBPs) and residual free available chlorine (FAC) can pose risks to subsequent biological treatment processes. This study aims to propose a method based on H₂O₂ regulation to convert the reactive species in the EO system from a FAC-dominated regime to a ¹O₂-contributing regime, inhibiting the production of Cl-DBPs and eliminating FAC residues. After treatment by the EO system, the BOD₅/COD ratio of the water sample increased from 0.15 to 0.49. Under the regulation of H₂O₂, the concentration of Cl-DBPs in the effluent of the EO system diminished from 14.02 mg/L to 2.88 mg/L, while the inhibition rate of luminous bacteria reduced from 95.97 % to 19.80 %. Moreover, sludge derived from the effluent of the EO system treated with H₂O₂ exhibited superior performance and microbial community structure compared to sludge obtained from the original EO system effluent. It is proposed that H₂O₂ undergoes a series of reactions within the EO system: electron transfer occurs at the electrode surface, generating free radicals such as

{HO}_{2}^{\cdot}

and

O_{2}^{\cdot -}

. Free radical chain reactions and the rapid chemical reactions between H₂O₂ and FAC are the two pathways through which ¹O₂ is produced. It indicated that EO treatment improved the biodegradability and the addition of H₂O₂ reduced the biological toxicity of the effluent.

垃圾填埋场渗滤液生物处理出水（LLBE）的特点是含有高浓度氯离子和复杂的有机污染物，生物降解性差。利用电化学氧化（EO）对水样进行处理，可以提高水样的生物降解性。然而，氯化消毒副产物（Cl-DBPs）和剩余游离有效氯（FAC）的产生可能对随后的生物处理过程构成风险。本研究旨在提出一种基于H2O2调控的方法，将EO系统中的活性物质从以FAC为主的状态转化为贡献H2O2的状态，从而抑制cl - dbp的产生并消除FAC残留物。经EO系统处理后，水样的BOD5/COD比由0.15提高到0.49。在H2O2的调控下，EO系统出水Cl-DBPs浓度由14.02 mg/L降至2.88 mg/L，对发光菌的抑制率由95.97 %降至19.80 %。此外，与原始EO系统出水污泥相比，经H2O2处理的EO系统出水污泥表现出更优越的性能和微生物群落结构。提出H2O2在EO体系内发生一系列反应：在电极表面发生电子转移，产生HO2·和O2·-等自由基。自由基链反应和H2O2与FAC之间的快速化学反应是产生1O2的两种途径。结果表明，EO处理提高了出水的可生化性，H2O2的加入降低了出水的生物毒性。

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引用次数: 0

PFPeA exposure drives hepatoxicity and liver fibrosis via oxidative stress/Wnt5a-induced hepatocyte senescence PFPeA暴露通过氧化应激/ wnt5a诱导的肝细胞衰老驱动肝毒性和肝纤维化

IF 11.3 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials

Pub Date : 2026-01-09 DOI: 10.1016/j.jhazmat.2026.141085

Kaijie Ren , Yuanchang Peng , Huan Chen , Xueni Wang , Tianhao Min , Yuyi Ma , Xiaoyuan Deng , Shiyao Tong , Yuanyuan Liu , Yong Zhang , Kun Zhu , Chengxue Dang , Benhua Sun , Hao Zhang , Wei Wang , Tuanhe Sun

Perfluoropentanoic acid (PFPeA), one of short-chain PFASs, has raised concern due to existence in the environment. The hepatotoxicity of PFPeA remains to be further elucidated. Cellular senescence, a distinct cellular state that occurs under stress conditions, plays a vital role in the progression of various diseases. However, the contribution of cellular senescence to PFPeA induced liver toxicity remains unclear. Our study investigated the specific role of cellular senescence in PFPeA induced liver injury and its underlying mechanisms in C57BL/6 J mice and THLE-2 cells. Our results demonstrated that PFPeA exposure at 5 μg/ml significantly induces hepatocyte senescence and liver injury, and fibrosis in mice. Further experiments revealed that PFPeA triggers excessive ROS production in hepatocytes, which leads to sustained activation of the Wnt5a signaling pathway, subsequently inducing cellular senescence and senescence-associated secretory phenotype (SASP) secretion, and ultimately resulting in liver dysfunction and fibrosis. Combination therapy targeting both senescence and Wnt5a significantly alleviated PFPeA -induced liver injury. In summary, our study provides compelling evidence highlighting the important role of cellular senescence in PFPeA induced liver injury. This research broadens our understanding of PFPeA induced hepatotoxicity and provides potential therapeutic and preventive strategies for managing PFPeA-related liver injury.

全氟戊酸（PFPeA）是一种短链全氟磺酸，由于其存在于环境中而引起人们的关注。PFPeA的肝毒性有待进一步研究。细胞衰老是应激条件下发生的一种独特的细胞状态，在各种疾病的发展中起着至关重要的作用。然而，细胞衰老在PFPeA诱导的肝毒性中的作用尚不清楚。我们研究了细胞衰老在PFPeA诱导的C57BL/6 J小鼠和THLE-2细胞肝损伤中的具体作用及其潜在机制。结果表明，5 μg/ml的PFPeA可显著诱导小鼠肝细胞衰老、肝损伤和肝纤维化。进一步实验发现，PFPeA可触发肝细胞过量ROS生成，导致Wnt5a信号通路持续激活，进而诱导细胞衰老和衰老相关分泌表型（senescence associated secretory phenotype， SASP）分泌，最终导致肝功能障碍和纤维化。针对衰老和Wnt5a联合治疗可显著减轻PFPeA诱导的肝损伤。总之，我们的研究提供了令人信服的证据，强调了细胞衰老在PFPeA诱导的肝损伤中的重要作用。本研究拓宽了我们对PFPeA肝毒性的认识，并为PFPeA相关肝损伤的治疗和预防提供了潜在的策略。

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引用次数: 0

Air pollution drives community assembly of abundant and rare microbial taxa in urban PM2.5 空气污染促使城市PM2.5中丰富而稀有的微生物类群群落聚集

IF 11.3 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials

Pub Date : 2026-01-09 DOI: 10.1016/j.jhazmat.2026.141074

Yuanyuan Pan, Xin Ke, Qiang Chen, Xinping Xiao, Zhiwei Zuo

Environmental microbial communities typically exhibit unbalanced distribution, characterized by a few abundant species coexisting with a multitude of rare species. However, few attentions were paid to the abundant and rare taxa in airborne microbial community. Here, we explored the distribution pattern, assembly mechanisms, and ecological roles of airborne bacterial and fungal communities, including pathogens. Daily sampling was conducted over a one-month period in Nanchang, China. Our results found out the uneven distribution of airborne microbial community. Compared with rare taxa, abundant taxa, exhibited broader niche widths, more ubiquitous distribution and stronger correlations with environmental factors. Stochastic processes dominated the assembly of abundant taxa, whereas deterministic processes governed the assembly of rare taxa. Air pollutants (PM_2.5, PM₁₀, NO₂, etc.) were identified as key factors regulating the assembly of bacterial subcommunities, but had no significant influence on that of fungal subcommunities. Co-occurrence network analysis indicated that abundant species played a more critical role in maintaining the community stability in the atmosphere. Potential pathogens were mainly distributed in abundant taxa. Notably, dominant pathogens like Staphylococcus aureus (0.62 %) and Nigrospora sps. (15.72 %) were unpredicted by neutral community model and showed strong correlations with environmental factors. Overall, our findings provide insights into the ecological mechanisms structuring airborne microbial communities, with particular implications for understanding pathogenic microorganisms.

环境微生物群落通常呈现不平衡分布的特点，即少数丰富的物种与大量稀有的物种共存。然而，空气微生物群落中丰富而稀有的类群却很少受到重视。在此，我们探讨了空气中细菌和真菌群落（包括病原体）的分布格局、组装机制和生态作用。在中国南昌市进行了为期一个月的每日抽样。我们的结果发现空气微生物群落分布不均匀。与稀有类群相比，丰富类群的生态位宽度更宽，分布更普遍，与环境因子的相关性更强。丰富类群的组合受随机过程的支配，而稀有类群的组合受确定性过程的支配。空气污染物（PM2.5、PM10、NO2等）是调节细菌亚群落聚集的关键因子，但对真菌亚群落的影响不显著。共现网络分析表明，丰富的物种在维持群落稳定中起着更为关键的作用。潜在病原体主要分布在丰富的分类群中。值得注意的是，优势病原体如金黄色葡萄球菌（0.62 %）和黑孢菌。（15.72 %）与环境因子有较强的相关性。总的来说，我们的研究结果为构建空气微生物群落的生态机制提供了见解，对理解致病微生物具有特殊意义。

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引用次数: 0

Fusarium oxysporum f. sp. niveum invasion promotes Pseudomonas-driven antibiotic resistance gene enrichment 尖孢镰刀菌入侵促进假单胞菌驱动的抗生素耐药基因富集

IF 11.3 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials

Pub Date : 2026-01-09 DOI: 10.1016/j.jhazmat.2026.141084

Haiyan Wu , Jiahui Shen , Hongyuan Zhang , Qirui Fang , Tongtong Zhu , Jun Yuan , Qirong Shen , Chao Xue

The dissemination of antibiotic resistance genes (ARGs) in agricultural soils poses a growing threat to ecosystem health and food security, highlighting the need to identify key environmental drivers. Although soil-borne phytopathogens disrupt microbial communities, their specific impact on ARG dynamics remains poorly understood. In this study, we examined how Fusarium oxysporum f. sp. niveum (FON) invasion changes soil ARG profiles. Our results indicate that increasing FON loads heighten the abundances of soil ARGs, virulence factor genes (VFGs), and mobile genetic elements (MGEs). This invasion significantly transformed rhizosphere bacterial communities by enriching Pseudomonas populations. We further identified Pseudomonas-driven mechanisms that involve functional adaptations such as SOS response activation and enhanced biofilm formation. Genomic features, including vertically inherited ARGs (e.g., mexF/T/W, bacA) and horizontal gene transfer (HGT) elements tnpA transpositions, along with ecological interactions such as growth-supporting metabolic exchanges, collectively fueled ARG enrichment and dissemination. Pseudomonas strains exhibited prevalent multidrug resistance (MDR) and further promoted the enrichment of antibiotic-resistant bacteria (ARB) through growth - supporting metabolic interactions. An analysis of 689 Pseudomonas genomes from diverse habitats revealed that nearly all genomes contain multiple ARGs, VFGs, and MGEs, suggesting that Pseudomonas proliferation significantly contributes to ARG dissemination. Our findings establish FON load as a critical driver of resistome dissemination by selectively enriching multi-resistant Pseudomonas reservoirs and activating key resistance-enhancing mechanisms within these reservoirs. These results offer mechanistic insights for managing antimicrobial resistance risks in agroecosystems.

抗生素耐药基因（ARGs）在农业土壤中的传播对生态系统健康和粮食安全构成越来越大的威胁，这凸显了确定关键环境驱动因素的必要性。虽然土壤传播的植物病原体会破坏微生物群落，但它们对ARG动态的具体影响仍知之甚少。本文研究了镰刀菌（Fusarium oxysporum f. sp. niveum， FON）入侵对土壤ARG谱的影响。结果表明，随着FON负荷的增加，土壤ARGs、毒力因子基因（vfg）和移动遗传因子（MGEs）的丰度增加。这种入侵通过丰富假单胞菌种群显著地改变了根际细菌群落。我们进一步确定了假单胞菌驱动的机制，包括功能适应，如SOS反应激活和增强的生物膜形成。基因组特征，包括垂直遗传的ARG（如mexF/T/W， bacA）和水平基因转移（HGT）元件tnpA转位，以及支持生长的代谢交换等生态相互作用，共同促进了ARG的富集和传播。假单胞菌表现出普遍的多药耐药（MDR），并通过支持生长的代谢相互作用进一步促进耐药菌（ARB）的富集。对来自不同生境的689个假单胞菌基因组的分析表明，几乎所有假单胞菌基因组都含有多个ARGs、vfg和MGEs，这表明假单胞菌的增殖对ARG的传播有重要作用。我们的研究结果表明，通过选择性地富集多耐药假单胞菌库并激活这些库中的关键抗性增强机制，FON负荷是抗性组传播的关键驱动因素。这些结果为管理农业生态系统中抗菌素耐药性风险提供了机制见解。

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引用次数: 0

Ultra-high SERS-active nanowires-PTFE composite substrate for trace detection of Aflatoxin B1 in aquaculture water 超高sers活性纳米线- ptfe复合衬底用于水产养殖水中黄曲霉毒素B1的痕量检测。

IF 11.3 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials

Pub Date : 2026-01-09 DOI: 10.1016/j.jhazmat.2026.141103

Liu Wang , Jie Han , Liangyan Sun , Jinru Feng , Xiaohan Lin , Daoliang Li , Cong Wang

Aflatoxin B₁ (AFB₁), a potential carcinogen distributed in aquaculture systems, threatens aquatic health and food safety. However, current methods for detecting AFB1 in aquaculture water still rely on time-consuming sample pretreatment and costly large-scale instruments. Herein, we developed a high-performance surface-enhanced Raman scattering (SERS) sensor based on ultra-active silver nanowires (U-AgNWs) for AFB₁ quantification in aquaculture water. PTFE substrate with hydrophobic condensation effect were selected to enhance SERS signal. Self-assembled AgNWs at the three-phase (oil-water-gas) interface were added onto the PTFE substrate. Subsequently, AgNWs were etched with aqua regia to increase surface roughness. Following iterative refinement, a novel PTFE substrate with remarkable SERS activity was successfully fabricated. This hybrid structure with significant SERS enhancement had been validated through FDTD simulation, and subsequent experiments had confirmed that the sensor had anti-interference, reproducibility, and repeatability. The sensor exhibited excellent linearity (R² > 0.97) for AFB₁ within the range of 0.1ng/mL-10⁴ng/mL and had satisfactory performance in aquaculture with recoveries of 97.3 %-103.2 % (reference HPLC/LM-MS). This sensor provides a simple, highly sensitive, and selective strategy for the timely detection of AFB₁ in aquaculture, thus contributing to the security of aquatic products, the safeguarding of ecosystems, and the protection of public health. And this work also paved a way for developing facile devices to detect antibiotics, heavy metals, and other targets.

黄曲霉毒素B1 （AFB1）是一种分布在水产养殖系统中的潜在致癌物，威胁着水产健康和食品安全。然而，目前检测水产养殖水体中AFB1的方法仍然依赖于耗时的样品预处理和昂贵的大型仪器。在此，我们开发了一种基于超活性银纳米线（U-AgNWs）的高性能表面增强拉曼散射（SERS）传感器，用于水产养殖水中AFB1的定量分析。选择具有疏水凝结效应的聚四氟乙烯基片增强SERS信号。将三相（油水-气）界面自组装的AgNWs添加到聚四氟乙烯基板上。随后，用王水蚀刻AgNWs以增加表面粗糙度。经过反复改进，成功制备了具有显著SERS活性的新型PTFE基板。通过FDTD仿真验证了这种具有明显SERS增强的混合结构，随后的实验证实了该传感器具有抗干扰性、再现性和可重复性。该传感器对AFB1在0.1ng/mL ~ 104ng/mL范围内具有良好的线性关系（R²> 0.97），在水产养殖中具有良好的效果，回收率为97.3% % ~ 103.2 %（参考HPLC/LM-MS）。该传感器为水产养殖中AFB1的及时检测提供了一种简单、高灵敏度和选择性的策略，从而为水产品的安全、生态系统的保护和公众健康的保护做出了贡献。这项工作也为开发检测抗生素、重金属和其他目标的简易设备铺平了道路。

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引用次数: 0