Pub Date : 2025-04-22DOI: 10.1016/j.jhazmat.2025.138383
Huang Yu , Dandan Zhang , Rui Xiong , Shengwei Liu , Ruiwen Hu , Pubo Chen , Xiaoyan Wu , Hantong Zou , Nan Hu , Dexin Ding , Qingyun Yan , Zhili He
Uranium (U) can impact microbially driven soil phosphorus (P) and carbon (C) cycling. However, the response of microbial P and C turnover to U in different soils is not well understood. Through the quantitative assay of P pools and soil organic C (SOC) quantitative assay and sequencing of 16S rRNA gene amplicons and metagenomes, we investigated the effect of U on P and C biotransformation in grassland (GL), paddy soil (PY), forest soil (FT). U (60 mg kg−1) impacted the diversity, interaction and stability of soil bacterial communities, leading to a decrease in available P (AP). Under U stress, organophosphate mineralization substantially contributed to the AP in GL and FT, whereas intracellular P metabolism dominated the AP in PY. Also, the reductive citrate cycle (rTCA cycle) promoted the content of SOC in GL, while the rTCA cycle and complex organic C degradation pathways enhanced the SOC in PY and FT, respectively. Notably, functional bacteria carrying organic C degradation genes could decompose SOC to enhance soil AP. Bacteria developed various resistance strategies to cope with U stress. This study reveals soil-dependent response of microbial P and C cycling and its ecological functions under the influence of radioactive contaminants in different soil systems.
{"title":"Soil-dependent responses of bacterial communities, phosphorus and carbon turnover to uranium stress in different soil ecosystems","authors":"Huang Yu , Dandan Zhang , Rui Xiong , Shengwei Liu , Ruiwen Hu , Pubo Chen , Xiaoyan Wu , Hantong Zou , Nan Hu , Dexin Ding , Qingyun Yan , Zhili He","doi":"10.1016/j.jhazmat.2025.138383","DOIUrl":"10.1016/j.jhazmat.2025.138383","url":null,"abstract":"<div><div>Uranium (U) can impact microbially driven soil phosphorus (P) and carbon (C) cycling. However, the response of microbial P and C turnover to U in different soils is not well understood. Through the quantitative assay of P pools and soil organic C (SOC) quantitative assay and sequencing of 16S rRNA gene amplicons and metagenomes, we investigated the effect of U on P and C biotransformation in grassland (GL), paddy soil (PY), forest soil (FT). U (60 mg kg<sup>−1</sup>) impacted the diversity, interaction and stability of soil bacterial communities, leading to a decrease in available P (AP). Under U stress, organophosphate mineralization substantially contributed to the AP in GL and FT, whereas intracellular P metabolism dominated the AP in PY. Also, the reductive citrate cycle (rTCA cycle) promoted the content of SOC in GL, while the rTCA cycle and complex organic C degradation pathways enhanced the SOC in PY and FT, respectively. Notably, functional bacteria carrying organic C degradation genes could decompose SOC to enhance soil AP. Bacteria developed various resistance strategies to cope with U stress. This study reveals soil-dependent response of microbial P and C cycling and its ecological functions under the influence of radioactive contaminants in different soil systems.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138383"},"PeriodicalIF":12.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143857690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Inoculating functional bacterial strains is a cost-effective strategy for enhancing treatment of anaerobic digestion liquids in swine wastewater. This study systematically evaluated inoculation of heterotrophic nitrification aerobic denitrification strain Acinetobacter indicus CZH-5 in an internal circulation airlift zeolite sphere-based sequencing batch reactor (IR) for aerobic removal of nitrogen (N), phosphorus (P), and tetracycline (TEC). Inoculation with CZH-5 promoted secretion of quorum sensing signaling molecules, specifically N-acyl-homoserine lactones (C6-HSL and C10-HSL). These signaling molecules enhance quorum sensing and reinforce cooperation among functional bacteria. Under optimal conditions, average removal efficiencies of total nitrogen, total phosphate, and TEC were 92.8%, 88.4%, and 93.1%, respectively. The removal performance in IR exceeded that of the control by 26%–71%. N removal involved complete nitrification-denitrification, while accumulated P was transformed into phosphate monoesters within biofilm. Metagenomic analysis identified Thauera and Acinetobacter as the dominant genera, and Acinetobacter indicus as predominant species. Inoculation enhanced microbial richness and diversity to improve system operational stability. The abundance of functional genes associated with N, P, and TEC transformations significantly increased compared to the control. This study aimed to investigate the characteristics and mechanisms of inoculating a heterotrophic nitrification aerobic denitrification strain into an aerated biofilm system for swine wastewater remediation.
{"title":"Inoculation with Acinetobacter indicus CZH-5 in internal circulation airlift zeolite spheres sequencing batch reactor to augment simultaneous removal of nitrogen, phosphorus, and tetracycline","authors":"Zuhao Chen, Yongyou Hu, Guanglei Qiu, Donghui Liang, Jianhua Cheng, Yuancai Chen, Guobin Wang, Xiaoqiang Zhu, Jieyun Xie","doi":"10.1016/j.jhazmat.2025.138384","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.138384","url":null,"abstract":"Inoculating functional bacterial strains is a cost-effective strategy for enhancing treatment of anaerobic digestion liquids in swine wastewater. This study systematically evaluated inoculation of heterotrophic nitrification aerobic denitrification strain <em>Acinetobacter indicus</em> CZH-5 in an internal circulation airlift zeolite sphere-based sequencing batch reactor (IR) for aerobic removal of nitrogen (N), phosphorus (P), and tetracycline (TEC). Inoculation with CZH-5 promoted secretion of quorum sensing signaling molecules, specifically N-acyl-homoserine lactones (C6-HSL and C10-HSL). These signaling molecules enhance quorum sensing and reinforce cooperation among functional bacteria. Under optimal conditions, average removal efficiencies of total nitrogen, total phosphate, and TEC were 92.8%, 88.4%, and 93.1%, respectively. The removal performance in IR exceeded that of the control by 26%–71%. N removal involved complete nitrification-denitrification, while accumulated P was transformed into phosphate monoesters within biofilm. Metagenomic analysis identified <em>Thauera</em> and <em>Acinetobacter</em> as the dominant genera, and <em>Acinetobacter indicus</em> as predominant species. Inoculation enhanced microbial richness and diversity to improve system operational stability. The abundance of functional genes associated with N, P, and TEC transformations significantly increased compared to the control. This study aimed to investigate the characteristics and mechanisms of inoculating a heterotrophic nitrification aerobic denitrification strain into an aerated biofilm system for swine wastewater remediation.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"67 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-22DOI: 10.1016/j.jhazmat.2025.138373
Tra My Bui Thi, Tao Chen, Tao Luo, Yann Leroux, Khalil Hanna, Jean-François Boily
Mn(III) species play critical roles in determining the environmental fate of antibiotics released into natural systems. Their reactivity is, however, strongly influenced by complexation reactions with (in)organic ligands. This study investigates the impact of Mn(III) complexation with pyrophosphate (PP), a model environmental ligand, on the redox-driven degradation of ciprofloxacin (CIP), a widely used antibiotic and environmental contaminant. Spectroscopic analysis and thermodynamic modeling revealed that Mn(III)-PP complexes initially dissociate into MnOH2+ species, which then undergo disproportionation to form MnO2 colloids. Both dissociation and disproportionation processes had comparable trends at pH 4 and 7, with reactivities that were strongly dependent on Mn(III):PP ratios. The progress of CIP oxidation by Mn compounds over time was sigmoidal, with an initial lag phase attributed to Mn(III)-PP complexes dissociation and disproportionation. CIP degradation was predominantly governed by pH, with maximal rate constants decreasing from k=0.390 h-1 at pH 3 and k=0.065 h-1 at pH 5, and no CIP removal under circumneutral to alkaline conditions. Cyclic voltammetry also suggested that pH values strongly altered the redox potential of the Mn(III)/Mn(II) couple. These collective findings indicated that ligand complexation, such as with PP, enhanced Mn(III) stability and mitigated dissociation and disproportionation reactions. The new insight provided by this work on the speciation and redox activity of Mn(III) should thereby be considered for understanding ciprofloxacin degradation in contaminated water systems.
{"title":"Ligand-limited oxidation of ciprofloxacin by Mn(III)","authors":"Tra My Bui Thi, Tao Chen, Tao Luo, Yann Leroux, Khalil Hanna, Jean-François Boily","doi":"10.1016/j.jhazmat.2025.138373","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.138373","url":null,"abstract":"Mn(III) species play critical roles in determining the environmental fate of antibiotics released into natural systems. Their reactivity is, however, strongly influenced by complexation reactions with (in)organic ligands. This study investigates the impact of Mn(III) complexation with pyrophosphate (PP), a model environmental ligand, on the redox-driven degradation of ciprofloxacin (CIP), a widely used antibiotic and environmental contaminant. Spectroscopic analysis and thermodynamic modeling revealed that Mn(III)-PP complexes initially dissociate into MnOH<sup>2+</sup> species, which then undergo disproportionation to form MnO<sub>2</sub> colloids. Both dissociation and disproportionation processes had comparable trends at pH 4 and 7, with reactivities that were strongly dependent on Mn(III):PP ratios. The progress of CIP oxidation by Mn compounds over time was sigmoidal, with an initial lag phase attributed to Mn(III)-PP complexes dissociation and disproportionation. CIP degradation was predominantly governed by pH, with maximal rate constants decreasing from k=0.390<!-- --> <!-- -->h<sup>-1</sup> at pH 3 and k=0.065<!-- --> <!-- -->h<sup>-1</sup> at pH 5, and no CIP removal under circumneutral to alkaline conditions. Cyclic voltammetry also suggested that pH values strongly altered the redox potential of the Mn(III)/Mn(II) couple. These collective findings indicated that ligand complexation, such as with PP, enhanced Mn(III) stability and mitigated dissociation and disproportionation reactions. The new insight provided by this work on the speciation and redox activity of Mn(III) should thereby be considered for understanding ciprofloxacin degradation in contaminated water systems.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"29 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-22DOI: 10.1016/j.jhazmat.2025.138371
Lukas Kogler , Sarah Stellnberger , Verena Schwingenschlögl-Maisetschläger , Lisa Aichinger , Verena Kopatz , Andreas H. Teuschl-Woller , Lukas Kenner , Verena Pichler
Microplastics comprise a heterogeneous group of polymer particles that vary in chemical properties, size, and shape, that may influence their environmental and in vivo behavior. Numerous in vitro and in vivo studies show induction of oxidative stress and metabolic disturbances. Valid critique regarding unrealistically high concentrations or additives within standard materials calls some results into question. Here, we present a novel protocol for the detergent-free production of polyethylene terephthalate (PET) and biodegradable poly(butylenadipat-co-terephthalat) (PBAT) micro- and nanoplastic particles (MNPs) as model microplastics for research. The particles were produced by dissolution precipitation from trifluoroacetic acid (TFA) for PET or tetrahydrofuran (THF)/ethanol for PBAT. Different PET sources were investigated for MNPs production. PET MNPs in the size range of 170–1000 nm with up to 80 % yield were produced from pellets as starting material. Particle size can be adjusted by ultrasounding. The non-toxic concentration range for two commonly used detergents was assessed by means of MTT assay. PET particles with a Zeta-potential of −45 were stable in aqueous suspension with and without detergents at neutral pH. Biodegradable PBAT particles in the micro- and nanometer range were produced by adapting the PET precipitation protocol. These high-yield production protocols provide additive-free authentic PET and PBAT MNPs for research.
{"title":"Production of detergent-free PET and biodegradable PBAT micro- and nanoplastics","authors":"Lukas Kogler , Sarah Stellnberger , Verena Schwingenschlögl-Maisetschläger , Lisa Aichinger , Verena Kopatz , Andreas H. Teuschl-Woller , Lukas Kenner , Verena Pichler","doi":"10.1016/j.jhazmat.2025.138371","DOIUrl":"10.1016/j.jhazmat.2025.138371","url":null,"abstract":"<div><div>Microplastics comprise a heterogeneous group of polymer particles that vary in chemical properties, size, and shape, that may influence their environmental and <em>in vivo</em> behavior. Numerous <em>in vitro</em> and <em>in vivo</em> studies show induction of oxidative stress and metabolic disturbances. Valid critique regarding unrealistically high concentrations or additives within standard materials calls some results into question. Here, we present a novel protocol for the detergent-free production of polyethylene terephthalate (PET) and biodegradable poly(butylenadipat-co-terephthalat) (PBAT) micro- and nanoplastic particles (MNPs) as model microplastics for research. The particles were produced by dissolution precipitation from trifluoroacetic acid (TFA) for PET or tetrahydrofuran (THF)/ethanol for PBAT. Different PET sources were investigated for MNPs production. PET MNPs in the size range of 170–1000 nm with up to 80 % yield were produced from pellets as starting material. Particle size can be adjusted by ultrasounding. The non-toxic concentration range for two commonly used detergents was assessed by means of MTT assay. PET particles with a Zeta-potential of −45 were stable in aqueous suspension with and without detergents at neutral pH. Biodegradable PBAT particles in the micro- and nanometer range were produced by adapting the PET precipitation protocol. These high-yield production protocols provide additive-free authentic PET and PBAT MNPs for research.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138371"},"PeriodicalIF":12.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-22DOI: 10.1016/j.jhazmat.2025.138348
Bowei Ouyang, Ziyue Lv, Cui Gan, Cong Yang, Lei Tong, Jianbo Shi
Microbe-mediated iron cycling plays a pivotal role in biogeochemical processes. However, the impact of trace-level antibiotics on microbially mediated dissimilatory iron reduction (DIR) remains unexplored. In this study, we investigated the effects of four typical antibiotics on DIR mediated by Shewanella oneidensis MR-1, with a focus on their inhibitory effects on three extracellular electron transfer (EET) pathways: 1) direct EET, 2) soluble redox mediator-dependent EET, and 3) nanowire-mediated EET. Our findings demonstrate a concentration-dependent decline in DIR activity with increasing ceftizoxime concentrations, culminating in complete suppression at 64 μg/L. Polymyxin disrupts the cell membrane, causing structural damage that subsequently impairs the electron transport chain (ETC), leading to a reversible reduction in DIR activity. In contrast, ofloxacin and tetracycline directly down-regulated genes associated with electron production and transfer, thereby suppressing both electron transport system activity and the synthesis of NADH dehydrogenase and c-type cytochromes. This irreversibly disrupts ETC function, blocking S. oneidensis MR-1 from conducting EET and impairing DIR activity. This finding reveals antibiotic-induced alterations in microbial iron metabolism and provides new insights into their potential impact on the environmental iron cycling.
{"title":"Molecular mechanisms of antibiotic inhibition on microbial dissimilatory iron reduction","authors":"Bowei Ouyang, Ziyue Lv, Cui Gan, Cong Yang, Lei Tong, Jianbo Shi","doi":"10.1016/j.jhazmat.2025.138348","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.138348","url":null,"abstract":"Microbe-mediated iron cycling plays a pivotal role in biogeochemical processes. However, the impact of trace-level antibiotics on microbially mediated dissimilatory iron reduction (DIR) remains unexplored. In this study, we investigated the effects of four typical antibiotics on DIR mediated by <em>Shewanella oneidensis</em> MR-1, with a focus on their inhibitory effects on three extracellular electron transfer (EET) pathways: 1) direct EET, 2) soluble redox mediator-dependent EET, and 3) nanowire-mediated EET. Our findings demonstrate a concentration-dependent decline in DIR activity with increasing ceftizoxime concentrations, culminating in complete suppression at 64<!-- --> <!-- -->μg/L. Polymyxin disrupts the cell membrane, causing structural damage that subsequently impairs the electron transport chain (ETC), leading to a reversible reduction in DIR activity. In contrast, ofloxacin and tetracycline directly down-regulated genes associated with electron production and transfer, thereby suppressing both electron transport system activity and the synthesis of NADH dehydrogenase and c-type cytochromes. This irreversibly disrupts ETC function, blocking <em>S. oneidensis</em> MR-1 from conducting EET and impairing DIR activity. This finding reveals antibiotic-induced alterations in microbial iron metabolism and provides new insights into their potential impact on the environmental iron cycling.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"47 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-22DOI: 10.1016/j.jhazmat.2025.138369
Pinxi Zhou , Mengzhu Wang , Shane DuBay , Yiwei Cao , Shangmingyu Zhang , Jiayu Zhang , Zhengrui Hu , Zhixiong Yang , Yibo Wang , Xiaoying Zhao , Lin Sun , Jiachen Dang , Xingcheng He , Yongjie Wu
Widespread pollution of microplastics (MPs) and nanoplastics (NPs) poses significant threats to organisms and human health. However, the extent of MPs and NPs contamination and their ecological risks to wildlife remain underexplored. In this study, we used Laser Direct Infrared (LDIR) spectroscopy to identify and characterize MPs in the intestinal contents of 49 bird species, and Pyrolysis-Gas Chromatography/Mass Spectrometry (Py-GC/MS) to identify NPs in the intestinal contents of five species. LDIR analysis indicated that chlorinated polyethylene (CPE) and polyvinyl chloride (PVC) were the most prevalent plastics among 32 identified types. MP particle sizes below 100 μm were most abundant, and MPs were predominantly in the form of fragments or pellets. We also found that birds with narrower dietary niche breadth had more MPs. Herbivorous and carnivorous birds had higher MP abundance than omnivorous species, which suggests the capacity of MP accumulation across diet categories. The Polymer Hazard Index (PHI) for MPs revealed that most species sampled were classified at hazard levels III or IV. Py-GC/MS identified four types of NPs in bird intestines, including nylon 66 (PA66), PVC, polyethylene (PE), and polypropylene (PP). This study advances our knowledge of plastic pollution ingested by terrestrial organisms and the risks associated with increased plastic pollution in the environment.
{"title":"Widespread microplastic and nanoplastic contamination in the intestines of birds: A case study from Chengdu, China","authors":"Pinxi Zhou , Mengzhu Wang , Shane DuBay , Yiwei Cao , Shangmingyu Zhang , Jiayu Zhang , Zhengrui Hu , Zhixiong Yang , Yibo Wang , Xiaoying Zhao , Lin Sun , Jiachen Dang , Xingcheng He , Yongjie Wu","doi":"10.1016/j.jhazmat.2025.138369","DOIUrl":"10.1016/j.jhazmat.2025.138369","url":null,"abstract":"<div><div>Widespread pollution of microplastics (MPs) and nanoplastics (NPs) poses significant threats to organisms and human health. However, the extent of MPs and NPs contamination and their ecological risks to wildlife remain underexplored. In this study, we used Laser Direct Infrared (LDIR) spectroscopy to identify and characterize MPs in the intestinal contents of 49 bird species, and Pyrolysis-Gas Chromatography/Mass Spectrometry (Py-GC/MS) to identify NPs in the intestinal contents of five species. LDIR analysis indicated that chlorinated polyethylene (CPE) and polyvinyl chloride (PVC) were the most prevalent plastics among 32 identified types. MP particle sizes below 100 μm were most abundant, and MPs were predominantly in the form of fragments or pellets. We also found that birds with narrower dietary niche breadth had more MPs. Herbivorous and carnivorous birds had higher MP abundance than omnivorous species, which suggests the capacity of MP accumulation across diet categories. The Polymer Hazard Index (PHI) for MPs revealed that most species sampled were classified at hazard levels III or IV. Py-GC/MS identified four types of NPs in bird intestines, including nylon 66 (PA66), PVC, polyethylene (PE), and polypropylene (PP). This study advances our knowledge of plastic pollution ingested by terrestrial organisms and the risks associated with increased plastic pollution in the environment.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138369"},"PeriodicalIF":12.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Studies on the association between nitrogen dioxide (NO2) exposure and out-of-hospital cardiac arrest (OHCA) are limited and present inconsistent results. In the context of global urbanization, a large population is exposed to high ambient NO2 pollution, highlighting the need for further clarification of NO2-related health hazards. Furthermore, previous studies mostly applied exposure data from monitoring stations, with relatively few investigations examining acute effects using high-resolution modeled data. To explore the association between NO2 and cardiac OHCA onset risk, a time-stratified case-crossover study was conducted using data from emergency medical service (EMS) systems across 23 Chinese provinces throughout 2020. Conditional logistic regression models were used to investigate the potential association between NO2 and OHCA onset. Individual-level NO2 data from both models and monitoring stations were analyzed separately to evaluate their comparability in practice. The analysis incorporated 76,263 EMS-attended cardiac OHCA onsets. The health estimates from NO2 predictions and measurements were comparable without statistically significant differences, with each 10 µg/m3 increase associated with a 1.16% (95% confidence interval [CI]: 0.38-1.94%] and 1.03% (95% CI: 0.37-1.69%] increase in the risk of OHCA onset, respectively. This nationwide multicenter study demonstrated adverse effects of NO2 exposure on OHCA onset in a large population residing in regions with higher and variable NO2 levels. These findings contribute robust epidemiological evidence to this field and offer new evidence to support global policymaking, particularly in developing countries. Additionally, modeled NO2 predictions with high resolution and coverage can serve as effective alternatives to traditional monitoring station data in epidemiological studies.
{"title":"Association between nitrogen dioxide exposure and out-of-hospital cardiac arrest onset in China: A multicenter, time-stratified, case-crossover study","authors":"Chang Pan, Xinyue Li, Xuan Zhang, Jiaqi Zheng, Ruixue Song, Ziyang Zhang, Renjie Chen, Haidong Kan, Feng Xu, Yuguo Chen, Xia Meng","doi":"10.1016/j.jhazmat.2025.138341","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.138341","url":null,"abstract":"Studies on the association between nitrogen dioxide (NO<sub>2</sub>) exposure and out-of-hospital cardiac arrest (OHCA) are limited and present inconsistent results. In the context of global urbanization, a large population is exposed to high ambient NO<sub>2</sub> pollution, highlighting the need for further clarification of NO<sub>2</sub>-related health hazards. Furthermore, previous studies mostly applied exposure data from monitoring stations, with relatively few investigations examining acute effects using high-resolution modeled data. To explore the association between NO<sub>2</sub> and cardiac OHCA onset risk, a time-stratified case-crossover study was conducted using data from emergency medical service (EMS) systems across 23 Chinese provinces throughout 2020. Conditional logistic regression models were used to investigate the potential association between NO<sub>2</sub> and OHCA onset. Individual-level NO<sub>2</sub> data from both models and monitoring stations were analyzed separately to evaluate their comparability in practice. The analysis incorporated 76,263 EMS-attended cardiac OHCA onsets. The health estimates from NO<sub>2</sub> predictions and measurements were comparable without statistically significant differences, with each 10<!-- --> <!-- -->µg/m<sup>3</sup> increase associated with a 1.16% (95% confidence interval [CI]: 0.38-1.94%] and 1.03% (95% CI: 0.37-1.69%] increase in the risk of OHCA onset, respectively. This nationwide multicenter study demonstrated adverse effects of NO<sub>2</sub> exposure on OHCA onset in a large population residing in regions with higher and variable NO<sub>2</sub> levels. These findings contribute robust epidemiological evidence to this field and offer new evidence to support global policymaking, particularly in developing countries. Additionally, modeled NO<sub>2</sub> predictions with high resolution and coverage can serve as effective alternatives to traditional monitoring station data in epidemiological studies.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"3 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-22DOI: 10.1016/j.jhazmat.2025.138388
Hanxun Zou, Guoming Lin, Yuxuan Bao, Jie Liang, Yunyun Li, Lifeng Cai, Han-Ying Wu, Fan Yang, Hang Chen, Xiuchun Lin, Yi-Hong Xiao, Lei Yang, Jianhui Huang, Yu Hai
Hydrazine is a commonly used chemical in various industries, including pharmaceuticals, agriculture, and aerospace. However, its high toxicity may cause serious harm to the natural environments and human health. The development of new methods for sensitive and selective detection of hydrazine is of great significance. In this study, we present a fluorescent probe that employs a unique two-step excited-state intramolecular proton transfer (ESIPT) activation mechanism for hydrazine detection. This probe integrates a phthalimide group into benzothiazole scaffold with its fluorescence initially quenched due to the inhibition of ESIPT process and photo-induced electron transfer (OFF state). Upon exposure to hydrazine, the nucleophilic cleavage of the phthalimide group activates the first ESIPT process, yielding yellow emission (ON1 state). A subsequent deprotection step triggers the second ESIPT process, producing blue fluorescence (ON2 state). These three states fluorescent change along with dual-emission signal output provide a highly sensitive and reliable method for hydrazine detection and monitoring, with a limit of detection (LOD) of 18 nM. Moreover, this probe showed versatile applications in environmental monitoring, food sample analysis, plant imaging, and bioimaging, including a convenient smartphone-assisted quantitative assay. The dual-activation mechanism offers valuable insights for the design of novel ESIPT probes, paving the way for promoting their applications in chemical, biological, and environmental fields.
{"title":"Dual-Emission Fluorescent Probe with Sequential Two-Step ESIPT Activation Mechanism for Selective Hydrazine Detection and Multifunctional Applications","authors":"Hanxun Zou, Guoming Lin, Yuxuan Bao, Jie Liang, Yunyun Li, Lifeng Cai, Han-Ying Wu, Fan Yang, Hang Chen, Xiuchun Lin, Yi-Hong Xiao, Lei Yang, Jianhui Huang, Yu Hai","doi":"10.1016/j.jhazmat.2025.138388","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.138388","url":null,"abstract":"Hydrazine is a commonly used chemical in various industries, including pharmaceuticals, agriculture, and aerospace. However, its high toxicity may cause serious harm to the natural environments and human health. The development of new methods for sensitive and selective detection of hydrazine is of great significance. In this study, we present a fluorescent probe that employs a unique two-step excited-state intramolecular proton transfer (ESIPT) activation mechanism for hydrazine detection. This probe integrates a phthalimide group into benzothiazole scaffold with its fluorescence initially quenched due to the inhibition of ESIPT process and photo-induced electron transfer (OFF state). Upon exposure to hydrazine, the nucleophilic cleavage of the phthalimide group activates the first ESIPT process, yielding yellow emission (ON1 state). A subsequent deprotection step triggers the second ESIPT process, producing blue fluorescence (ON2 state). These three states fluorescent change along with dual-emission signal output provide a highly sensitive and reliable method for hydrazine detection and monitoring, with a limit of detection (LOD) of 18<!-- --> <!-- -->nM. Moreover, this probe showed versatile applications in environmental monitoring, food sample analysis, plant imaging, and bioimaging, including a convenient smartphone-assisted quantitative assay. The dual-activation mechanism offers valuable insights for the design of novel ESIPT probes, paving the way for promoting their applications in chemical, biological, and environmental fields.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"108 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microplastics (MPs) could interact with heavy metals via multiple mechanisms in subsurface environment. Understanding the effect of MPs on the fate of heavy metals is essential for the prediction of their ecological impacts. In this study, laboratory columns were conducted to investigate the effects of pristine/aged secondary polystyrene fragments of different sizes and dosages on the transport of Pb2+ in saturated porous media. MPs generally promoted Pb2+ mobility, and the promotion degree was greater with increasing MP size and dosage. The enhancement of Pb2+ mobility by naturally aged MPs was slightly weaker than that by pristine MPs. The sensitivity of Pb2+ mobility to MPs was correlated with the flow velocity, the promotion impact was more significant at larger flow velocity. The enhanced MPs mobility of Pb2+ was mainly attributed to the decrease Pb2+sorption capacity of the media resulting from dilution effect, as well as alterations in pore structure and porosity caused by the high heterogeneity of MP fragments. Findings of this study indicated that the fate and transport of heavy metals are strongly influenced by the co-contamination with MPs. Evaluation of their cotransport in the subsurface is essential for accurately predicting their environmental risks.
{"title":"Effects of polystyrene fragments on the transport of Pb2+ in saturated porous media: the role of microplastics characteristics and flow velocity","authors":"Yining Ji, Kaiwen Yang, Xiaohui Li, Lizhu Wang, Hongxia Xu, Jichun Wu","doi":"10.1016/j.jhazmat.2025.138362","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.138362","url":null,"abstract":"Microplastics (MPs) could interact with heavy metals via multiple mechanisms in subsurface environment. Understanding the effect of MPs on the fate of heavy metals is essential for the prediction of their ecological impacts. In this study, laboratory columns were conducted to investigate the effects of pristine/aged secondary polystyrene fragments of different sizes and dosages on the transport of Pb<sup>2+</sup> in saturated porous media. MPs generally promoted Pb<sup>2+</sup> mobility, and the promotion degree was greater with increasing MP size and dosage. The enhancement of Pb<sup>2+</sup> mobility by naturally aged MPs was slightly weaker than that by pristine MPs. The sensitivity of Pb<sup>2+</sup> mobility to MPs was correlated with the flow velocity, the promotion impact was more significant at larger flow velocity. The enhanced MPs mobility of Pb<sup>2+</sup> was mainly attributed to the decrease Pb<sup>2+</sup>sorption capacity of the media resulting from dilution effect, as well as alterations in pore structure and porosity caused by the high heterogeneity of MP fragments. Findings of this study indicated that the fate and transport of heavy metals are strongly influenced by the co-contamination with MPs. Evaluation of their cotransport in the subsurface is essential for accurately predicting their environmental risks.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"24 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143857692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-22DOI: 10.1016/j.jhazmat.2025.138342
Dan Ai , Tao Wu , Deying Huang , Zeguo Ying , Jibiao Zhang
Microalgal-fungal symbiotic systems (MFSS) have emerged as a promising approach for wastewater treatment, yet the mechanisms driving reactive oxygen species (ROS) generation and pharmaceutical pollutant removal remain underexplored. This study investigates the synergistic interactions within MFSS and their role in Mn(II) oxidation, with a focus on enhancing carbamazepine (CBZ) degradation and microbial community dynamics. The results reveal that microalgal-fungal interactions inhibit Fe-S cluster activity, disrupting electron transport chains and promoting extracellular superoxide production. This superoxide surge directly accelerates Mn(II) oxidation, while Mn(III) and ROS drive synergistic effects to amplify CBZ removal efficiency. Notably, system-specific variations in superoxide generation were observed across different MFSS configurations, determining their degradation performance. Water quality factors, such as microbial community complexity and nitrate concentration, play crucial roles in CBZ degradation in natural water systems. High-throughput sequencing reveals dynamic shifts in bacterial and eukaryotic communities, highlighting their synergistic interactions in pollutant degradation. Temporal and spatial changes in microbial community structure suggest that the system evolves into a more adaptive configuration during pollutant treatment, enhancing long-term stability. These findings advance the mechanistic understanding of ROS-mediated pollutant degradation in MFSS and provide actionable strategies for optimizing bioremediation systems in engineered and natural water environments.
{"title":"Enhanced removal of carbamazepine by microalgal-fungal symbiotic systems in the presence of Mn(II): Synergistic mechanisms and microbial community dynamics","authors":"Dan Ai , Tao Wu , Deying Huang , Zeguo Ying , Jibiao Zhang","doi":"10.1016/j.jhazmat.2025.138342","DOIUrl":"10.1016/j.jhazmat.2025.138342","url":null,"abstract":"<div><div>Microalgal-fungal symbiotic systems (MFSS) have emerged as a promising approach for wastewater treatment, yet the mechanisms driving reactive oxygen species (ROS) generation and pharmaceutical pollutant removal remain underexplored. This study investigates the synergistic interactions within MFSS and their role in Mn(II) oxidation, with a focus on enhancing carbamazepine (CBZ) degradation and microbial community dynamics. The results reveal that microalgal-fungal interactions inhibit Fe-S cluster activity, disrupting electron transport chains and promoting extracellular superoxide production. This superoxide surge directly accelerates Mn(II) oxidation, while Mn(III) and ROS drive synergistic effects to amplify CBZ removal efficiency. Notably, system-specific variations in superoxide generation were observed across different MFSS configurations, determining their degradation performance. Water quality factors, such as microbial community complexity and nitrate concentration, play crucial roles in CBZ degradation in natural water systems. High-throughput sequencing reveals dynamic shifts in bacterial and eukaryotic communities, highlighting their synergistic interactions in pollutant degradation. Temporal and spatial changes in microbial community structure suggest that the system evolves into a more adaptive configuration during pollutant treatment, enhancing long-term stability. These findings advance the mechanistic understanding of ROS-mediated pollutant degradation in MFSS and provide actionable strategies for optimizing bioremediation systems in engineered and natural water environments.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138342"},"PeriodicalIF":12.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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