As emerging pollutants, bisphenol A (BPA), tetrabromobisphenol A (TBBPA) and its analogs have become widespread in the coastal environment of China. To investigate the occurrence of these novel contaminants in Chinese marginal sea, 176 seawater and 88 sediment samples were collected from the Yellow Sea and East China Sea. In seawater and sediment, the detection rates of TBBPA are 83.9% and 100%, BPA and 20 analogs were within 1.7%-93.7% and 1.1%-100%, respectively. In seawater, the concentrations of TBBPA and analogs were significantly higher in winter than in summer. But in sediment, there were no significant seasonal differences. The distribution of targets in 28 sampling points of the Yellow River and Yangtze River showed that industrial point source emissions have a greater impact on concentration. Fugacity analysis showed that BPA tends to diffuse from seawater to sediment while the TBBPA did the opposite. The maximum hazard quotients (HQ) of TBBPA and its analogs for three aquatic organisms indicated that they have high ecological risks, especially for complex organisms. Five suspected metabolites were identified by non-targeted screening. This study provides novel insights into the pollution status, dispersal behavior, and ecological risk of TBBPA and its analogs in the marine environment.
{"title":"Exploring Factors Influencing the Spatial Distribution and Seasonal Changes of BPA, TBBPA, and 20 analogs in China’s Marginal Seas","authors":"Mengxin Xu, Minggang Zheng, Ke Ning, Ruixia Yang, Ling Wang, Aifeng Liu, Guangbo Qu","doi":"10.1016/j.jhazmat.2025.137209","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137209","url":null,"abstract":"As emerging pollutants, bisphenol A (BPA), tetrabromobisphenol A (TBBPA) and its analogs have become widespread in the coastal environment of China. To investigate the occurrence of these novel contaminants in Chinese marginal sea, 176 seawater and 88 sediment samples were collected from the Yellow Sea and East China Sea. In seawater and sediment, the detection rates of TBBPA are 83.9% and 100%, BPA and 20 analogs were within 1.7%-93.7% and 1.1%-100%, respectively. In seawater, the concentrations of TBBPA and analogs were significantly higher in winter than in summer. But in sediment, there were no significant seasonal differences. The distribution of targets in 28 sampling points of the Yellow River and Yangtze River showed that industrial point source emissions have a greater impact on concentration. Fugacity analysis showed that BPA tends to diffuse from seawater to sediment while the TBBPA did the opposite. The maximum hazard quotients (<em>HQ</em>) of TBBPA and its analogs for three aquatic organisms indicated that they have high ecological risks, especially for complex organisms. Five suspected metabolites were identified by non-targeted screening. This study provides novel insights into the pollution status, dispersal behavior, and ecological risk of TBBPA and its analogs in the marine environment.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"51 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142974872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-14DOI: 10.1016/j.jhazmat.2025.137223
Ying Zhang, Ming-Zhi Shen, Jian-Xia Wang, Jing-Han Wang, Zhan-You Chi
Microalgae-based wastewater treatment could realize simultaneous nutrients recovery and CO2 sequestration. However, impacts of environmental microplastics (MPs) and antibiotic co-exposure on microalgal growth, nutrients removal, intracellular nitric oxide (NO) accumulation and subsequent nitrous oxide (N2O) emission are unclarified, which could greatly offset the CO2 sequestration benefit. To reveal the potential impacts of environmental concentrations of MPs and antibiotic co-exposure on microalgal greenhouse gas mitigation, this study investigated the effects of representative MPs (PE, PVC, PA), antibiotic sulfamethoxazole (SMX), and nitrite (NO2--N) in various combinations on attached Chlorella sorokiniana growth, nutrients removal, anti-oxidative responses, and N2O emission originated from intracellular NO build-up. Microalgal biofilm growth was more inhibited under 10 μg/L MPs than 100 μg/L SMX, and MPs+SMX co-exposure displayed toxicity antagonism while MPs+MPs co-exposure caused toxicity synergism (up to 66% growth inhibition). Extracellular polysaccharides content correlated well with microalgal biofilm density under various stresses, while SMX involved stresses displayed chlorophyll a content reduction. Microalgal assimilation and MPs adsorption contributed to nutrients removal, and phosphorus removal displayed less variance among different stresses (residual phosphorus <0.5 mg/L) than nitrogen. Intracellular NO conversion to N2O almost doubled during the co-exposure processes, and N2O emission under NO2--N+PE+PVC co-exposure could offset the contribution of microalgal CO2 sequestration by as high as 176.2%. Results of this study appealed for urgent concern regarding environmental MPs and antibiotic co-exposure on primary producers’ growth characteristics and their greenhouse gas mitigation properties.
{"title":"Less toxic combined microplastics exposure towards attached Chlorella sorokiniana in the presence of sulfamethoxazole while massive microalgal nitrous oxide emission under multiple stresses","authors":"Ying Zhang, Ming-Zhi Shen, Jian-Xia Wang, Jing-Han Wang, Zhan-You Chi","doi":"10.1016/j.jhazmat.2025.137223","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137223","url":null,"abstract":"Microalgae-based wastewater treatment could realize simultaneous nutrients recovery and CO<sub>2</sub> sequestration. However, impacts of environmental microplastics (MPs) and antibiotic co-exposure on microalgal growth, nutrients removal, intracellular nitric oxide (NO) accumulation and subsequent nitrous oxide (N<sub>2</sub>O) emission are unclarified, which could greatly offset the CO<sub>2</sub> sequestration benefit. To reveal the potential impacts of environmental concentrations of MPs and antibiotic co-exposure on microalgal greenhouse gas mitigation, this study investigated the effects of representative MPs (PE, PVC, PA), antibiotic sulfamethoxazole (SMX), and nitrite (NO<sub>2</sub><sup>-</sup>-N) in various combinations on attached <em>Chlorella sorokiniana</em> growth, nutrients removal, anti-oxidative responses, and N<sub>2</sub>O emission originated from intracellular NO build-up. Microalgal biofilm growth was more inhibited under 10<!-- --> <!-- -->μg/L MPs than 100<!-- --> <!-- -->μg/L SMX, and MPs+SMX co-exposure displayed toxicity antagonism while MPs+MPs co-exposure caused toxicity synergism (up to 66% growth inhibition). Extracellular polysaccharides content correlated well with microalgal biofilm density under various stresses, while SMX involved stresses displayed chlorophyll a content reduction. Microalgal assimilation and MPs adsorption contributed to nutrients removal, and phosphorus removal displayed less variance among different stresses (residual phosphorus <0.5<!-- --> <!-- -->mg/L) than nitrogen. Intracellular NO conversion to N<sub>2</sub>O almost doubled during the co-exposure processes, and N<sub>2</sub>O emission under NO<sub>2</sub><sup>-</sup>-N+PE+PVC co-exposure could offset the contribution of microalgal CO<sub>2</sub> sequestration by as high as 176.2%. Results of this study appealed for urgent concern regarding environmental MPs and antibiotic co-exposure on primary producers’ growth characteristics and their greenhouse gas mitigation properties.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"76 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142974873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The cyclic triangular complex – silver (I) 4-nitro-3,5-bis(trifluoromethyl)pyrazolate (Ag3pz3) with super π-acidity shows great potential in adsorptive desulfurization (ADS) as a novel adsorbent, however, it fails to work well in the continue flow adsorption study. In order to improve its dynamic adsorption performance, a composite has been prepared by mixing Ag3pz3 and multilayer graphene (MG) in methanol. Based on the results of characterization by FT-IR, XPS, SEM, and so on, the optimal mass ratio of Ag3pz3:MG in the synthesis is 0.14, so composite obtained under this condition is labeled as Ag3pz3/MG-0.14, in which the molecules of Ag3pz3 are uniformly distributed on the surface of MG via Ag∙∙∙C interactions and π-π stacking. The composite exhibits higher specific surface area than that of Ag3pz3. Importantly, the column test proves the practicality of the composite – Ag3pz3/MG-0.14 in continuous flow desulfurization with the enhanced dynamic capacity (4.0 mg S/g), in comparison with 1.9 mg S/g for graphene (C0 = 200 mg S/L for dibenzothiophene in iso-octane, madsorbent = 0.5 g, T = 298 K, v = 0.1 mL∙min-1). This work confirms the importance and advantage of the integration of functional coordination compound and suitable supporting materials for designing new type of high-performance ADS adsorbents.
{"title":"Facile manufacture of silver(I) complex/multilayer graphene composite for dynamic adsorption desulfurization","authors":"Yujin Zhong, Xiaoquan Feng, Limin Ma, Yongxin Bi, Wenhua Zhang, Hongwei Hou, Guang Yang","doi":"10.1016/j.jhazmat.2025.137220","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137220","url":null,"abstract":"The cyclic triangular complex – silver (I) 4-nitro-3,5-bis(trifluoromethyl)pyrazolate (<strong>Ag</strong><sub><strong>3</strong></sub><strong>pz</strong><sub><strong>3</strong></sub>) with super π-acidity shows great potential in adsorptive desulfurization (ADS) as a novel adsorbent, however, it fails to work well in the continue flow adsorption study. In order to improve its dynamic adsorption performance, a composite has been prepared by mixing <strong>Ag</strong><sub><strong>3</strong></sub><strong>pz</strong><sub><strong>3</strong></sub> and multilayer graphene (MG) in methanol. Based on the results of characterization by FT-IR, XPS, SEM, and so on, the optimal mass ratio of <strong>Ag</strong><sub><strong>3</strong></sub><strong>pz</strong><sub><strong>3</strong></sub>:MG in the synthesis is 0.14, so composite obtained under this condition is labeled as <strong>Ag</strong><sub><strong>3</strong></sub><strong>pz</strong><sub><strong>3</strong></sub><strong>/MG</strong>-0.14, in which the molecules of <strong>Ag</strong><sub><strong>3</strong></sub><strong>pz</strong><sub><strong>3</strong></sub> are uniformly distributed on the surface of MG <em>via</em> Ag∙∙∙C interactions and π-π stacking. The composite exhibits higher specific surface area than that of <strong>Ag</strong><sub><strong>3</strong></sub><strong>pz</strong><sub><strong>3</strong></sub>. Importantly, the column test proves the practicality of the composite – <strong>Ag</strong><sub><strong>3</strong></sub><strong>pz</strong><sub><strong>3</strong></sub><strong>/MG</strong>-0.14 in continuous flow desulfurization with the enhanced dynamic capacity (4.0<!-- --> <!-- -->mg S/g), in comparison with 1.9<!-- --> <!-- -->mg S/g for graphene (C<sub>0</sub> = 200<!-- --> <!-- -->mg S/L for dibenzothiophene in <em>iso</em>-octane, m<sub>adsorbent</sub> = 0.5<!-- --> <!-- -->g, T = 298<!-- --> <!-- -->K, <em>v</em> = 0.1<!-- --> <!-- -->mL∙min<sup>-1</sup>). This work confirms the importance and advantage of the integration of functional coordination compound and suitable <span><span>supporting materials</span></span> for designing new type of high-performance ADS adsorbents.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"22 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142974881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-13DOI: 10.1016/j.jhazmat.2025.137213
Boyu Yang, Luning Sun, Zheng Peng, Qing Zhang, Mei Lin, Zhilin Peng, Jue Yang, Lan Zheng
Rare earth elements are integral to modern technology, but their increasing environmental distribution due to anthropogenic activities poses potential health risks to humans. This study utilized zebrafish as a model to assess developmental and locomotor performance effects of Europium and Samarium. Exposure to Eu or Sm induced a reduction in heart rate, growth inhibition, and morphological deformities. RNA-Seq revealed gene expression alterations linked to critical biological processes and functions following Eu or Sm exposure. Impaired organogenesis in liver and exocrine pancreas, evident through fluorescence imaging, was confirmed transcriptionally. Exposure to Eu or Sm significantly impaired the burst and spontaneous swimming behaviors of zebrafish larvae, characterized by pronounced reductions in movement distance, frequency, and velocity. These observations indicate severe locomotor dysfunction in zebrafish exposed to Eu and Sm. The comprehensive downregulation of the oxidative phosphorylation pathway is likely a primary factor contributing to these motor impairments. Apoptosis induced by Eu and Sm, confirmed through acridine orange staining, was accompanied by the upregulation of the intrinsic apoptosis pathway. Our findings contribute critical insights into the health risks of rare earth elements, informing risk assessment and management strategies.
{"title":"Toxicity of Rare Earth Elements Europium and Samarium on Zebrafish Development and Locomotor Performance","authors":"Boyu Yang, Luning Sun, Zheng Peng, Qing Zhang, Mei Lin, Zhilin Peng, Jue Yang, Lan Zheng","doi":"10.1016/j.jhazmat.2025.137213","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137213","url":null,"abstract":"Rare earth elements are integral to modern technology, but their increasing environmental distribution due to anthropogenic activities poses potential health risks to humans. This study utilized zebrafish as a model to assess developmental and locomotor performance effects of Europium and Samarium. Exposure to Eu or Sm induced a reduction in heart rate, growth inhibition, and morphological deformities. RNA-Seq revealed gene expression alterations linked to critical biological processes and functions following Eu or Sm exposure. Impaired organogenesis in liver and exocrine pancreas, evident through fluorescence imaging, was confirmed transcriptionally. Exposure to Eu or Sm significantly impaired the burst and spontaneous swimming behaviors of zebrafish larvae, characterized by pronounced reductions in movement distance, frequency, and velocity. These observations indicate severe locomotor dysfunction in zebrafish exposed to Eu and Sm. The comprehensive downregulation of the oxidative phosphorylation pathway is likely a primary factor contributing to these motor impairments. Apoptosis induced by Eu and Sm, confirmed through acridine orange staining, was accompanied by the upregulation of the intrinsic apoptosis pathway. Our findings contribute critical insights into the health risks of rare earth elements, informing risk assessment and management strategies.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"27 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-13DOI: 10.1016/j.jhazmat.2025.137216
Taeyeon Kim, Soyeon Eom, Moon-Kyung Kim, Kyung-Duk Zoh
Structural diversity can affect the degradability of per- and polyfluoroalkyl substances (PFASs) during treatment. Here, three PFASs with different functional groups—C6F13-R, PFHpA, PFHxS, and 6:2 FTS—were degraded using vacuum ultraviolet (VUV/UV)-based advanced reduction processes. While fully fluorinated PFASs—PFHpA and PFHxS—were degraded faster in the VUV/UV/sulfite reaction than in VUV/UV photolysis, VUV/UV photolysis was more effective for degrading 6:2 FTS by OH radicals produced. PFCAs such as PFHxA, PFPeA, and PFBA were formed by VUV/UV photolysis of PFHpA and 6:2 FTS, but the PFCA formation was inhibited in the VUV/UV/sulfite reaction. The degradation of the three PFASs in the VUV/UV/sulfite reaction was mainly carried out by H/F and SO3•/F exchange mechanisms, mediated by hydrated electrons (eaq-) produced in the reaction. During the VUV/UV/sulfite reaction, PFCA precursors were first formed as transformation products, which were further transformed into PFCAs by the following VUV/UV/H2O2 reaction, implying enhanced defluorination of 6:2 FTS. Our results indicate that VUV/UV-based treatments can be an option for PFAS degradation and defluorination by combining advanced reduction and oxidation processes and utilizing both eaq- and oxidative radicals.
{"title":"Degradation and Defluorination of C6F13 PFASs with Different Functional Groups by VUV/UV-based Reduction and Oxidation Processes","authors":"Taeyeon Kim, Soyeon Eom, Moon-Kyung Kim, Kyung-Duk Zoh","doi":"10.1016/j.jhazmat.2025.137216","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137216","url":null,"abstract":"Structural diversity can affect the degradability of per- and polyfluoroalkyl substances (PFASs) during treatment. Here, three PFASs with different functional groups—C<sub>6</sub>F<sub>13</sub>-R, PFHpA, PFHxS, and 6:2 FTS—were degraded using vacuum ultraviolet (VUV/UV)-based advanced reduction processes. While fully fluorinated PFASs—PFHpA and PFHxS—were degraded faster in the VUV/UV/sulfite reaction than in VUV/UV photolysis, VUV/UV photolysis was more effective for degrading 6:2 FTS by OH radicals produced. PFCAs such as PFHxA, PFPeA, and PFBA were formed by VUV/UV photolysis of PFHpA and 6:2 FTS, but the PFCA formation was inhibited in the VUV/UV/sulfite reaction. The degradation of the three PFASs in the VUV/UV/sulfite reaction was mainly carried out by H/F and SO<sub>3</sub><sup>•</sup>/F exchange mechanisms, mediated by hydrated electrons (e<sub>aq</sub><sup>-</sup>) produced in the reaction. During the VUV/UV/sulfite reaction, PFCA precursors were first formed as transformation products, which were further transformed into PFCAs by the following VUV/UV/H<sub>2</sub>O<sub>2</sub> reaction, implying enhanced defluorination of 6:2 FTS. Our results indicate that VUV/UV-based treatments can be an option for PFAS degradation and defluorination by combining advanced reduction and oxidation processes and utilizing both e<sub>aq</sub><sup>-</sup> and oxidative radicals.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"22 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142974884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-13DOI: 10.1016/j.jhazmat.2025.137197
Minwook Park, Young-Seong Kim, Seonghwan Kim, Joong Yeon Lim
This paper introduces a highly absorbent and sensitive cellulose nanofiber (CNF)/gold nanorod (GNR)@Ag surface-enhanced Raman scattering (SERS) sensor, fabricated using the vacuum filtration method. By optimizing the Ag thickness in the GNR@Ag core–shell structures and integrating them with CNFs, optimal SERS hotspots were identified using the Raman probe molecule 4-aminothiophenol (4-ATP). To concentrate pesticides extracted from fruit and vegetable surfaces, we utilized the evaporation enrichment effect using hydrophilic CNF and hole-punched hydrophobic polydimethylsiloxane (PDMS). This design leverages the hydrophilic substrate and localized evaporation to create a microfluidic flow that concentrates analytes within a small hole area, enhancing SERS sensitivity by up to 465%. The sensor achieved on-site detection limits for Thiram as low as 10-11 M on fruit surfaces, specifically apples and chili peppers. This approach underscores how localized molecule enrichment can substantially improve field-based pesticide analysis. the sensor’s response to interfering substances (e.g., glucose and citric acid) and other harmful molecules (e.g., carbendazim and nitrofurazone was also evaluated, demonstrating high sensitivity and accuracy). The PDMS-assisted CNF/GNR@Ag SERS sensor exhibits flexibility, ease of fabrication, and excellent sensitivity and selectivity, showing significant potential for applications in food safety, agriculture, and environmental monitoring. These advancements are anticipated to promote the practical adoption of SERS-based sensor technology across diverse fields, suggesting broad future utility.
{"title":"Ultra-Sensitive, On-Site Pesticide Detection for Environmental and Food Safety Monitoring using Flexible Cellulose Nano Fiber/Au Nanorod@Ag SERS Sensor","authors":"Minwook Park, Young-Seong Kim, Seonghwan Kim, Joong Yeon Lim","doi":"10.1016/j.jhazmat.2025.137197","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137197","url":null,"abstract":"This paper introduces a highly absorbent and sensitive cellulose nanofiber (CNF)/gold nanorod (GNR)@Ag surface-enhanced Raman scattering (SERS) sensor, fabricated using the vacuum filtration method. By optimizing the Ag thickness in the GNR@Ag core–shell structures and integrating them with CNFs, optimal SERS hotspots were identified using the Raman probe molecule 4-aminothiophenol (4-ATP). To concentrate pesticides extracted from fruit and vegetable surfaces, we utilized the evaporation enrichment effect using hydrophilic CNF and hole-punched hydrophobic polydimethylsiloxane (PDMS). This design leverages the hydrophilic substrate and localized evaporation to create a microfluidic flow that concentrates analytes within a small hole area, enhancing SERS sensitivity by up to 465%. The sensor achieved on-site detection limits for Thiram as low as 10<sup>-11<!-- --> </sup>M on fruit surfaces, specifically apples and chili peppers. This approach underscores how localized molecule enrichment can substantially improve field-based pesticide analysis. the sensor’s response to interfering substances (e.g., glucose and citric acid) and other harmful molecules (e.g., carbendazim and nitrofurazone was also evaluated, demonstrating high sensitivity and accuracy). The PDMS-assisted CNF/GNR@Ag SERS sensor exhibits flexibility, ease of fabrication, and excellent sensitivity and selectivity, showing significant potential for applications in food safety, agriculture, and environmental monitoring. These advancements are anticipated to promote the practical adoption of SERS-based sensor technology across diverse fields, suggesting broad future utility.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"1 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microplastics are pervasive environmental contaminants found across diverse ecosystems, inducing toxic effects in a wide range of organisms. However, the neurotoxic effects of thermally degraded polystyrene (T-PS) and its underlying mechanisms remain poorly unexplored. In this study, Caenorhabditis elegans was exposed to environmentally relevant concentrations of T-PS (0.1–100 μg/L), and endpoints including locomotion behaviors, neuronal development, neurotransmitter levels, and gene expression were assessed. Significant alterations in morphology, crystallinity, elemental composition, and functional groups were observed in T-PS compared to virgin polystyrene (V-PS), indicating that thermal degradation modifies the physicochemical properties of V-PS. Exposure to 10–100 μg/L T-PS resulted in a more pronounced decrease in head thrashes, body bends, forward turns, and backward turns compared to V-PS. In transgenic nematodes, T-PS exposure significantly impacted fluorescence intensity and the percentage of worms exhibiting neurodegeneration in serotonergic, cholinergic, dopaminergic, and γ-aminobutyric acid (GABA) neurons. Correspondingly, marked reductions were observed in the levels of dopamine, serotonin, GABA, and choline neurotransmitters, alongside significant declines in neurotransmitter-related gene expression (e.g., dat-1, tph-1, unc-30, and cha-1). Pearson’s correlation analysis revealed a significant positive association between these genes and locomotion behaviors. Furthermore, the absence of locomotion behavior impairment in dat-1 (ok157), tph-1 (mg280), unc-30 (e191), and cha-1 (e1152) mutants highlights the pivotal roles of these genes in mediating T-PS-induced neurotoxicity in C. elegans. This study enhances our understanding of the neurotoxic mechanisms of T-PS at environmental concentrations, providing valuable insights into its potential environmental health risks.
{"title":"Behavioral and Molecular Neurotoxicity of Thermally Degraded Polystyrene in Caenorhabditis elegans","authors":"Hui Li, Jinyu Chen, Chenyin Dong, Xiaoxia Chen, Yulun Gu, Yongqi Jiang, Jingwen Cui, Haibo Chen","doi":"10.1016/j.jhazmat.2025.137212","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137212","url":null,"abstract":"Microplastics are pervasive environmental contaminants found across diverse ecosystems, inducing toxic effects in a wide range of organisms. However, the neurotoxic effects of thermally degraded polystyrene (T-PS) and its underlying mechanisms remain poorly unexplored. In this study, <em>Caenorhabditis elegans</em> was exposed to environmentally relevant concentrations of T-PS (0.1–100<!-- --> <!-- -->μg/L), and endpoints including locomotion behaviors, neuronal development, neurotransmitter levels, and gene expression were assessed. Significant alterations in morphology, crystallinity, elemental composition, and functional groups were observed in T-PS compared to virgin polystyrene (V-PS), indicating that thermal degradation modifies the physicochemical properties of V-PS. Exposure to 10–100<!-- --> <!-- -->μg/L T-PS resulted in a more pronounced decrease in head thrashes, body bends, forward turns, and backward turns compared to V-PS. In transgenic nematodes, T-PS exposure significantly impacted fluorescence intensity and the percentage of worms exhibiting neurodegeneration in serotonergic, cholinergic, dopaminergic, and γ-aminobutyric acid (GABA) neurons. Correspondingly, marked reductions were observed in the levels of dopamine, serotonin, GABA, and choline neurotransmitters, alongside significant declines in neurotransmitter-related gene expression (e.g., <em>dat-1</em>, <em>tph-1</em>, <em>unc-30</em>, and <em>cha-1</em>). Pearson’s correlation analysis revealed a significant positive association between these genes and locomotion behaviors. Furthermore, the absence of locomotion behavior impairment in <em>dat-1 (ok157)</em>, <em>tph-1 (mg280)</em>, <em>unc-30 (e191)</em>, and <em>cha-1 (e1152)</em> mutants highlights the pivotal roles of these genes in mediating T-PS-induced neurotoxicity in <em>C. elegans</em>. This study enhances our understanding of the neurotoxic mechanisms of T-PS at environmental concentrations, providing valuable insights into its potential environmental health risks.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"27 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Liquid crystal monomers (LCMs) are emerging pollutants that have attracted attention recently due to their unique chemical properties and wide applications. However, in-depth research on LCMs' potential risks to soil health remains blank. Therefore, 107 LCMs and nine soil health characterization proteins/enzymes were selected as research objects in this study. A grading evaluation system for soil health toxicological effect indicators under LCMs exposure was constructed from five dimensions (i.e., soil animals, soil plants, soil microorganisms, soil carbon, nitrogen and phosphorus cycles, and human health) by molecular docking and molecular dynamics simulation methods. Priority control lists for soil health toxicological effects under LCMs exposure were developed based on the proposed evaluation system, with rationality verified through non-bonded interaction, 2D-QSAR and Meta-analysis. Results showed that 32, 56 and 19 LCMs presented unacceptable, potential, and acceptable soil health risks, respectively. The oxidative damage of LCMs to plant leaves, the toxicity to earthworm growth and development, and its effects on key enzymes of the soil nitrogen cycle were suggested to be the priority-attention indicators. This is the first study that provides theoretical support for revealing the toxicological effects of LCM exposure on soil health and relevant pollution control strategies.
Environmental Implication
(a) why the studied material should be considered "hazardous material".Liquid crystal monomers (LCMs), which produce significant quantities and have broad applications, have been widely present in various environmental media. LCMs are thus considered emerging organic pollutants with persistence, bioaccumulation, and toxicity, which are a non- negligible threat to ecological environments and human health.(b) how the work helps address environmental problems.In this study, a soil health toxicity grading system for LCMs and a priority control list were designed and constructed for the first time. The soil health toxicological effects of LCMs were revealed, and solid theoretical support for LCMs’ pollution management strategies was provided.
{"title":"Liquid crystal monomers in soil: Developing priority list based on the proposed soil health indicators","authors":"Tingzhi Xu, Qikun Pu, Luanxiao Wei, Yu Li, Xixi Li, Yong Niu, Xia Jiang","doi":"10.1016/j.jhazmat.2025.137161","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137161","url":null,"abstract":"Liquid crystal monomers (LCMs) are emerging pollutants that have attracted attention recently due to their unique chemical properties and wide applications. However, in-depth research on LCMs' potential risks to soil health remains blank. Therefore, 107 LCMs and nine soil health characterization proteins/enzymes were selected as research objects in this study. A grading evaluation system for soil health toxicological effect indicators under LCMs exposure was constructed from five dimensions (i.e., soil animals, soil plants, soil microorganisms, soil carbon, nitrogen and phosphorus cycles, and human health) by molecular docking and molecular dynamics simulation methods. Priority control lists for soil health toxicological effects under LCMs exposure were developed based on the proposed evaluation system, with rationality verified through non-bonded interaction, 2D-QSAR and Meta-analysis. Results showed that 32, 56 and 19 LCMs presented unacceptable, potential, and acceptable soil health risks, respectively. The oxidative damage of LCMs to plant leaves, the toxicity to earthworm growth and development, and its effects on key enzymes of the soil nitrogen cycle were suggested to be the priority-attention indicators. This is the first study that provides theoretical support for revealing the toxicological effects of LCM exposure on soil health and relevant pollution control strategies.<h3>Environmental Implication</h3><strong>(a) why the studied material should be considered \"hazardous material\".</strong>Liquid crystal monomers (LCMs), which produce significant quantities and have broad applications, have been widely present in various environmental media. LCMs are thus considered emerging organic pollutants with persistence, bioaccumulation, and toxicity, which are a non- negligible threat to ecological environments and human health.<strong>(b) how the work helps address environmental problems.</strong>In this study, a soil health toxicity grading system for LCMs and a priority control list were designed and constructed for the first time. The soil health toxicological effects of LCMs were revealed, and solid theoretical support for LCMs’ pollution management strategies was provided.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"16 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Controlling the spread of antibiotic-resistance genes (ARGs) under antibiotic stress has become an increasingly urgent issue. Microalgae possess the capability to remove antibiotics while concurrently inhibiting ARGs. Microalgae-bacteria systems can produce significant quantities of extracellular polymeric substances (EPS). However, the roles of EPS in the spread of ARGs have not been sufficiently explored, resulting in an insufficient understanding of the contribution of each EPS component and a lack of analysis on the complex interactions between EPS and ARGs. This study systematically explored the overlooked role of EPS in the transmission of ARGs within microalgae-bacteria systems. The current results showed that the potential of the microalgae-bacteria system for treating antibiotic wastewater. The tightly bound-EPS (TB-EPS) can acquire the higher absolute abundances of ARGs compared with the loosely bound-EPS (LB-EPS). The correlation coefficient between polysaccharides and TB-EPS ARGs was higher than that between polysaccharides and LB-EPS ARGs. The gene patterns of LB-EPS closely clustered with those of TB-EPS, while intracellular ARG gene patterns differed from both TB-EPS and LB-EPS. Metagenomic analyses indicated that the relative abundances of sul1 and sul2 were considerably higher at the beginning stage compared to the end stage. The abundance of Achromobacter, increased by the end stage, aligning with its potential to produce exopolysaccharide. Additionally, the absolute abundance of genes encoding exopolysaccharides (nagB and galE) and conjugative transfer transcription regulator (traF), increased over time. These findings enhanced our comprehension of the significance of EPS on the fate of ARGs in microalgae-bacteria systems during the treatment of antibiotic-contaminated wastewater.
Environmental Implication
Despite the increasing recognition of extracellular polymeric substances (EPS), their role in the transmission of antibiotic-resistance genes (ARGs) within microalgae-bacteria systems remains largely unexplored. This study aims to elucidate the physicochemical characteristics of EPS extracted from microalgae-bacteria system and perform multivariate analyses of EPS and ARGs. Results revealed that polysaccharides had a higher correlation coefficient with tightly bound-EPS (TB-EPS) ARGs compared to loosely bound-EPS (LB-EPS) ARGs. The genes patterns of LB-EPS were closely clustered with those of TB-EPS genes. This work can enhance our comprehension of the significance of EPS in the microalgae-bacteria system for managing the proliferation of ARGs.
{"title":"Overlooked role of extracellular polymeric substances in antibiotic-resistance gene transfer within microalgae-bacteria system","authors":"Shengnan Li, Yun Bai, Zhiling Li, Aijie Wang, Nan-Qi Ren, Shih-Hsin Ho","doi":"10.1016/j.jhazmat.2025.137206","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137206","url":null,"abstract":"Controlling the spread of antibiotic-resistance genes (ARGs) under antibiotic stress has become an increasingly urgent issue. Microalgae possess the capability to remove antibiotics while concurrently inhibiting ARGs. Microalgae-bacteria systems can produce significant quantities of extracellular polymeric substances (EPS). However, the roles of EPS in the spread of ARGs have not been sufficiently explored, resulting in an insufficient understanding of the contribution of each EPS component and a lack of analysis on the complex interactions between EPS and ARGs. This study systematically explored the overlooked role of EPS in the transmission of ARGs within microalgae-bacteria systems. The current results showed that the potential of the microalgae-bacteria system for treating antibiotic wastewater. The tightly bound-EPS (TB-EPS) can acquire the higher absolute abundances of ARGs compared with the loosely bound-EPS (LB-EPS). The correlation coefficient between polysaccharides and TB-EPS ARGs was higher than that between polysaccharides and LB-EPS ARGs. The gene patterns of LB-EPS closely clustered with those of TB-EPS, while intracellular ARG gene patterns differed from both TB-EPS and LB-EPS. Metagenomic analyses indicated that the relative abundances of <em>sul1</em> and <em>sul2</em> were considerably higher at the beginning stage compared to the end stage. The abundance of <em>Achromobacter</em>, increased by the end stage, aligning with its potential to produce exopolysaccharide. Additionally, the absolute abundance of genes encoding exopolysaccharides (<em>nagB</em> and <em>galE</em>) and conjugative transfer transcription regulator (<em>traF</em>), increased over time. These findings enhanced our comprehension of the significance of EPS on the fate of ARGs in microalgae-bacteria systems during the treatment of antibiotic-contaminated wastewater.<h3>Environmental Implication</h3>Despite the increasing recognition of extracellular polymeric substances (EPS), their role in the transmission of antibiotic-resistance genes (ARGs) within microalgae-bacteria systems remains largely unexplored. This study aims to elucidate the physicochemical characteristics of EPS extracted from microalgae-bacteria system and perform multivariate analyses of EPS and ARGs. Results revealed that polysaccharides had a higher correlation coefficient with tightly bound-EPS (TB-EPS) ARGs compared to loosely bound-EPS (LB-EPS) ARGs. The genes patterns of LB-EPS were closely clustered with those of TB-EPS genes. This work can enhance our comprehension of the significance of EPS in the microalgae-bacteria system for managing the proliferation of ARGs.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"28 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142975088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Polyhydroxybutyrate (PHB) has attracted attention as a representative polymer for biodegradable plastics produced by microorganisms. Since information regarding the fate of PHB released into the environment is limited, it is necessary to identify them based on metagenomic information. We estimated the PHB biodegradability in coastal water samples collected from 15 near shore sites around Japan using oxygen consumption as an indicator in laboratory-scale incubation experiments and conducted 16S rRNA gene-based microbial community profiling. The PHB-biodegradation-rate was significantly positively correlated with the diversity indices of the microbial community in seawater prior to incubation, indicating that seawater with higher diversity is more advantageous for biodegradation. We identified 41 operational taxonomic units exhibiting a significant positive correlation between their abundance and PHB-degradation-rates; these included several microorganisms with hitherto unreported PHB-degrading ability. Next, we analyzed gene expression patterns over incubation time using seawater samples employing metagenomic and metatranscriptomic approaches. Fifty-seven putative extracellular PHB/PHA depolymerase genes were found in 38 metagenomic bins and their expression changed with increasing biodegradation rates, indicating that PHB released into the marine environment is subject to degradation by phylogenetically diverse PHB-depolymerase-producing bacteria. These findings should contribute to expanding the knowledge on degradation of biodegradable plastics by complex marine microbial ecosystems.
{"title":"Metagenomic and metatranscriptomic analyses reveal uncharted microbial constituents responsible for polyhydroxybutyrate biodegradation in coastal waters","authors":"Kyohei Kuroda, Kyosuke Yamamoto, Rino Isshiki, Riho Tokizawa, Chisato Shiiba, Shodai Hino, Naoko Yamano, Erika Usui, Tomoyo Miyakawa, Takamasa Miura, Kei Kamino, Hideyuki Tamaki, Atsuyoshi Nakayama, Takashi Narihiro","doi":"10.1016/j.jhazmat.2025.137202","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137202","url":null,"abstract":"Polyhydroxybutyrate (PHB) has attracted attention as a representative polymer for biodegradable plastics produced by microorganisms. Since information regarding the fate of PHB released into the environment is limited, it is necessary to identify them based on metagenomic information. We estimated the PHB biodegradability in coastal water samples collected from 15 near shore sites around Japan using oxygen consumption as an indicator in laboratory-scale incubation experiments and conducted 16S rRNA gene-based microbial community profiling. The PHB-biodegradation-rate was significantly positively correlated with the diversity indices of the microbial community in seawater prior to incubation, indicating that seawater with higher diversity is more advantageous for biodegradation. We identified 41 operational taxonomic units exhibiting a significant positive correlation between their abundance and PHB-degradation-rates; these included several microorganisms with hitherto unreported PHB-degrading ability. Next, we analyzed gene expression patterns over incubation time using seawater samples employing metagenomic and metatranscriptomic approaches. Fifty-seven putative extracellular PHB/PHA depolymerase genes were found in 38 metagenomic bins and their expression changed with increasing biodegradation rates, indicating that PHB released into the marine environment is subject to degradation by phylogenetically diverse PHB-depolymerase-producing bacteria. These findings should contribute to expanding the knowledge on degradation of biodegradable plastics by complex marine microbial ecosystems.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"26 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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