Pub Date : 2025-04-21DOI: 10.1016/j.jhazmat.2025.138359
Michelle Morataya-Reyes , Aliro Villacorta , Javier Gutiérrez-García , Raquel Egea , Joan Martín-Pérez , Irene Barguilla , Ricard Marcos , Alba Hernández
This study examines the long-term impact of polyethylene terephthalate nanoplastics (PET-NPLs) and cigarette smoke condensate (CSC) on human lung BEAS-2B cells, focusing on key biological hallmarks of carcinogenesis. True-to-life PET-NPLs were generated from plastic water bottles and characterized to simulate environmental exposure conditions; and a comprehensive battery of assays was employed to assess genotoxicity, cellular transformation, and invasiveness. It was observed that, compared to passage control and individual exposures, co-exposure to PET-NPLs and CSC exacerbates oxidative stress, genotoxicity, and tumorigenic transformation, as evidenced by increased DNA damage, colony formation in soft agar, and enhanced cell migration and invasion. Transcriptomic analysis revealed a shift in cellular stress regulation including the upregulation of stress-response genes, including SLC7A11, NQO1, and HSPA1A, which are linked to oxidative stress adaptation and tumor survival. At the same time, key tumor-suppressor genes, such as LOX, and FN1, were significantly downregulated, promoting cellular transformation and invasiveness. These results provide compelling evidence that the combination of PET-NPLs and CSC enhances carcinogenic traits through oxidative stress, genomic instability, and disruption of tumor-suppressive pathways. This study underscores the importance of evaluating the synergistic effects of combined environmental exposures and their implications for human health.
{"title":"The long-term in vitro co-exposure of polyethylene terephthalate (PET) nanoplastics and cigarette smoke condensate exacerbates the induction of carcinogenic traits","authors":"Michelle Morataya-Reyes , Aliro Villacorta , Javier Gutiérrez-García , Raquel Egea , Joan Martín-Pérez , Irene Barguilla , Ricard Marcos , Alba Hernández","doi":"10.1016/j.jhazmat.2025.138359","DOIUrl":"10.1016/j.jhazmat.2025.138359","url":null,"abstract":"<div><div>This study examines the long-term impact of polyethylene terephthalate nanoplastics (PET-NPLs) and cigarette smoke condensate (CSC) on human lung BEAS-2B cells, focusing on key biological hallmarks of carcinogenesis. True-to-life PET-NPLs were generated from plastic water bottles and characterized to simulate environmental exposure conditions; and a comprehensive battery of assays was employed to assess genotoxicity, cellular transformation, and invasiveness. It was observed that, compared to passage control and individual exposures, co-exposure to PET-NPLs and CSC exacerbates oxidative stress, genotoxicity, and tumorigenic transformation, as evidenced by increased DNA damage, colony formation in soft agar, and enhanced cell migration and invasion. Transcriptomic analysis revealed a shift in cellular stress regulation including the upregulation of stress-response genes, including <em>SLC7A11, NQO1</em>, and <em>HSPA1A</em>, which are linked to oxidative stress adaptation and tumor survival. At the same time, key tumor-suppressor genes, such as <em>LOX,</em> and <em>FN1</em>, were significantly downregulated, promoting cellular transformation and invasiveness. These results provide compelling evidence that the combination of PET-NPLs and CSC enhances carcinogenic traits through oxidative stress, genomic instability, and disruption of tumor-suppressive pathways. This study underscores the importance of evaluating the synergistic effects of combined environmental exposures and their implications for human health.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138359"},"PeriodicalIF":12.2,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-21DOI: 10.1016/j.jhazmat.2025.138345
Qi Liu , Yao-Yao Ye , Sha Yang , Cheng-Yu Cui , Huan Du , Jin-Cheng Ye , Ji-Liang Cheng , Rui-Wen Hu , Lei Xiang , Bai-Lin Liu , Yan-Wen Li , Quan-Ying Cai , Miaoyue Zhang , Ce-Hui Mo , Hai-Ming Zhao
Butyl xanthate (BuX) is an emerging pollutant due to wide use as flotation collector, posing a serious threat to ecosystem health in mining areas. Here we develop a combinational plant-microbe remediation strategy for restoration of BuX-contaminated mining areas. A novel bacterial strain that completely degraded up to 1000 mg/L of BuX within 12 h was isolated and identified as Pseudomonas monteilii W50. It was found to harbor good tolerance to extreme environmental conditions and multiple plant growth-promoting traits such as phosphate and potassium solubilization, indole-3-acetic acid and gibberellin production, and cellulose degradation. This strain can colonize in the rhizosphere of an emergent macrophyte Cyperus alternifolius, improving removal of BuX and chemical oxygen demand (COD) from simulated wastewater. Compared to the phytoremediation alone, the removal of BuX and COD increased from 70 % to 98 % and from 21 % to 46 % respectively in the combined remediation The strain W50 protected the macrophyte from the phytotoxicity of BuX and the macrophyte provided it with a suitable habitat for return, benefiting each other. Compared to the individual treatment using C. alternifolius or strain W50, the combinational treatment significantly improved the plant growth and the residence of inoculated bacteria. Overall, C. alternifolius and strain W50 are the perfect combination for efficient and sustainable remediation of BuX-contaminated mine wastewater, overcoming the constraints of individual phytoremediation or bioaugmentation methods.
{"title":"Sustainable remediation of butyl xanthate-contaminated mine wastewater by combining emergent macrophyte Cyperus alternifolius with a versatile bacterial isolate","authors":"Qi Liu , Yao-Yao Ye , Sha Yang , Cheng-Yu Cui , Huan Du , Jin-Cheng Ye , Ji-Liang Cheng , Rui-Wen Hu , Lei Xiang , Bai-Lin Liu , Yan-Wen Li , Quan-Ying Cai , Miaoyue Zhang , Ce-Hui Mo , Hai-Ming Zhao","doi":"10.1016/j.jhazmat.2025.138345","DOIUrl":"10.1016/j.jhazmat.2025.138345","url":null,"abstract":"<div><div>Butyl xanthate (BuX) is an emerging pollutant due to wide use as flotation collector, posing a serious threat to ecosystem health in mining areas. Here we develop a combinational plant-microbe remediation strategy for restoration of BuX-contaminated mining areas. A novel bacterial strain that completely degraded up to 1000 mg/L of BuX within 12 h was isolated and identified as <em>Pseudomonas monteilii</em> W50. It was found to harbor good tolerance to extreme environmental conditions and multiple plant growth-promoting traits such as phosphate and potassium solubilization, indole-3-acetic acid and gibberellin production, and cellulose degradation. This strain can colonize in the rhizosphere of an emergent macrophyte <em>Cyperus alternifolius</em>, improving removal of BuX and chemical oxygen demand (COD) from simulated wastewater. Compared to the phytoremediation alone, the removal of BuX and COD increased from 70 % to 98 % and from 21 % to 46 % respectively in the combined remediation The strain W50 protected the macrophyte from the phytotoxicity of BuX and the macrophyte provided it with a suitable habitat for return, benefiting each other. Compared to the individual treatment using <em>C. alternifolius</em> or strain W50, the combinational treatment significantly improved the plant growth and the residence of inoculated bacteria. Overall, <em>C. alternifolius</em> and strain W50 are the perfect combination for efficient and sustainable remediation of BuX-contaminated mine wastewater, overcoming the constraints of individual phytoremediation or bioaugmentation methods.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138345"},"PeriodicalIF":12.2,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-21DOI: 10.1016/j.jhazmat.2025.138368
Hyunki Cho , Si-Eun Sung , Hyunsoo Lim , Seonyong Chung , Young Jun Kim , Heung-Bin Lim , Youngsam Kim
Cigarette filters are the most common form of litter worldwide and pose significant ecological risks because they degrade into microfibers and microplastics in aquatic environments. While previous studies have focused on the acute toxicity of cigarette leachate, the long-term ecological consequences of microplastic release from cigarette filters remain largely unexplored. This study evaluated the toxicity of cigarette filter-derived microplastics, including non-smoked cellulose acetate filters (CAF), smoked cigarette filters (GSF), on Daphnia magna, as well as leachate from smoked filter (LSF) for comparison. Imaging analysis confirmed that D. magna ingested cigarette filter-derived microplastics, which acted as carriers, gradually releasing harmful substances within organisms, a phenomenon consistent with the Trojan horse effect. Acute toxicity tests revealed similar 48-hour EC50 values (∼50 mg/L) for both GSF and LSF; however, GSF induced more pronounced long-term toxic effects. Chronic exposure to GSF significantly impairs reproduction, delays the timing of the first brood, reduces offspring size, and disrupts ecdysteroid-regulated genes. These findings indicate that cigarette filters are a persistent source of chemical pollution, threatening aquatic ecosystems. Specifically, microplastics from discarded cigarette filters act as Trojan horses, continuously releasing toxic chemicals and transporting hydrophobic contaminants, amplifying their environmental impact.
{"title":"Toxicological assessment of cigarette filter-derived microplastics in Daphnia magna","authors":"Hyunki Cho , Si-Eun Sung , Hyunsoo Lim , Seonyong Chung , Young Jun Kim , Heung-Bin Lim , Youngsam Kim","doi":"10.1016/j.jhazmat.2025.138368","DOIUrl":"10.1016/j.jhazmat.2025.138368","url":null,"abstract":"<div><div>Cigarette filters are the most common form of litter worldwide and pose significant ecological risks because they degrade into microfibers and microplastics in aquatic environments. While previous studies have focused on the acute toxicity of cigarette leachate, the long-term ecological consequences of microplastic release from cigarette filters remain largely unexplored. This study evaluated the toxicity of cigarette filter-derived microplastics, including non-smoked cellulose acetate filters (CAF), smoked cigarette filters (GSF), on <em>Daphnia magna</em>, as well as leachate from smoked filter (LSF) for comparison. Imaging analysis confirmed that <em>D. magna</em> ingested cigarette filter-derived microplastics, which acted as carriers, gradually releasing harmful substances within organisms, a phenomenon consistent with the Trojan horse effect. Acute toxicity tests revealed similar 48-hour EC<sub>50</sub> values (∼50 mg/L) for both GSF and LSF; however, GSF induced more pronounced long-term toxic effects. Chronic exposure to GSF significantly impairs reproduction, delays the timing of the first brood, reduces offspring size, and disrupts ecdysteroid-regulated genes. These findings indicate that cigarette filters are a persistent source of chemical pollution, threatening aquatic ecosystems. Specifically, microplastics from discarded cigarette filters act as Trojan horses, continuously releasing toxic chemicals and transporting hydrophobic contaminants, amplifying their environmental impact.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138368"},"PeriodicalIF":12.2,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-21DOI: 10.1016/j.jhazmat.2025.138358
Lulin Hu , Weiyan Yu , Yi Yang , Jingcheng Hao , Lu Xu
As a class of very common and important industrial liquid materials, metalworking fluids (MWFs) are easy to be vaporized into the air and cause pulmonary toxic effects. The inhaled MWF aerosols should first interact with the pulmonary surfactant (PS) film that plays an essential role in maintaining the normal respiratory mechanics and pulmonary immunology in a human body. Here, to probe any potential adverse impacts of airborne MWFs on the biophysical and physiological functions of PS film that may help achieve a deep and comprehensive understanding of the pulmonary toxicology of MWFs, a systematic study on the interaction between an animal-derived natural PS (i.e., Calsurf) film and the aerosols of water-based MWFs and their different constituents is conducted using constrained drop surfactometry (CDS) capable of closely simulating normal tidal breathing and lung-related physiological conditions in vitro. It was found that the airborne MWFs can induce strong PS inhibitions once their accumulated amount in the environment attains 0.2 mg/cm3. And their inhibitory effects are demonstrated to mainly originate from the surfactant additives such as polyethylene glycol monooleate (PEGMO) that can desorb PS film from the air/water interface via competitive interfacial adsorption, although it has normally been regarded as an eco-friendly commercial reagent.
{"title":"Surfactant additives in water-based metalworking fluids lead to strong biophysical inhibition of pulmonary surfactant film","authors":"Lulin Hu , Weiyan Yu , Yi Yang , Jingcheng Hao , Lu Xu","doi":"10.1016/j.jhazmat.2025.138358","DOIUrl":"10.1016/j.jhazmat.2025.138358","url":null,"abstract":"<div><div>As a class of very common and important industrial liquid materials, metalworking fluids (MWFs) are easy to be vaporized into the air and cause pulmonary toxic effects. The inhaled MWF aerosols should first interact with the pulmonary surfactant (PS) film that plays an essential role in maintaining the normal respiratory mechanics and pulmonary immunology in a human body. Here, to probe any potential adverse impacts of airborne MWFs on the biophysical and physiological functions of PS film that may help achieve a deep and comprehensive understanding of the pulmonary toxicology of MWFs, a systematic study on the interaction between an animal-derived natural PS (<em>i.e.</em>, Calsurf) film and the aerosols of water-based MWFs and their different constituents is conducted using constrained drop surfactometry (CDS) capable of closely simulating normal tidal breathing and lung-related physiological conditions <em>in vitro</em>. It was found that the airborne MWFs can induce strong PS inhibitions once their accumulated amount in the environment attains 0.2 mg/cm<sup>3</sup>. And their inhibitory effects are demonstrated to mainly originate from the surfactant additives such as polyethylene glycol monooleate (PEGMO) that can desorb PS film from the air/water interface <em>via</em> competitive interfacial adsorption, although it has normally been regarded as an eco-friendly commercial reagent.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138358"},"PeriodicalIF":12.2,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-21DOI: 10.1016/j.jhazmat.2025.138367
Qilong Li , Lin Zhu , Xiaoying Pu , Qi Sui , Xuemei Sun , Xinguo Zhao , Rongyuan Wang , Bin Xia
Marine microplastic (MP) pollution has emerged as a critical environmental issue, posing significant risks to a wide range of marine organisms. However, the trophic transfer of MPs with different characteristics among marine species remain poorly understood. This study investigated the abundances and characteristics of MPs in various fishery organisms from the Bohai Sea, China, and evaluated the influencing factors, trophic transfer and associated risks. Results showed that MP abundances ranged from 0.50 ± 0.52–2.38 ± 1.41 items per individual. Most MPs were smaller than 1 mm (74.6 %), with fiber being the most common shape (80.8 %), and transparent being the dominant color (75.3 %). Among the 13 identified polymer types, cellophane was the most prevalent. MP ingestion by fishery organisms was significantly influenced by body size, biological taxa, and habitat, while showing no correlation with feeding habits. Notably, the trophic transfer and significant biomagnification of MPs occurred through the marine food chain. Specially, MPs within the size ranges from 30 to 500 µm, in fibrous shape, transparent color, and composed of cellophane, PET, PP, and PA polymers, demonstrated enhanced potential for trophic transfer. PHI-based quantitative risk assessment revealed a medium to danger risk of MPs to marine fishery organisms, with hazard indices spanning medium to danger risk levels. These findings provide new insights into the bioaccumulation and risk of MPs across different trophic levels in marine organisms.
{"title":"Occurrence, trophic transfer and risk assessment of microplastics in fishery organisms from the Bohai Sea, China","authors":"Qilong Li , Lin Zhu , Xiaoying Pu , Qi Sui , Xuemei Sun , Xinguo Zhao , Rongyuan Wang , Bin Xia","doi":"10.1016/j.jhazmat.2025.138367","DOIUrl":"10.1016/j.jhazmat.2025.138367","url":null,"abstract":"<div><div>Marine microplastic (MP) pollution has emerged as a critical environmental issue, posing significant risks to a wide range of marine organisms. However, the trophic transfer of MPs with different characteristics among marine species remain poorly understood. This study investigated the abundances and characteristics of MPs in various fishery organisms from the Bohai Sea, China, and evaluated the influencing factors, trophic transfer and associated risks. Results showed that MP abundances ranged from 0.50 ± 0.52–2.38 ± 1.41 items per individual. Most MPs were smaller than 1 mm (74.6 %), with fiber being the most common shape (80.8 %), and transparent being the dominant color (75.3 %). Among the 13 identified polymer types, cellophane was the most prevalent. MP ingestion by fishery organisms was significantly influenced by body size, biological taxa, and habitat, while showing no correlation with feeding habits. Notably, the trophic transfer and significant biomagnification of MPs occurred through the marine food chain. Specially, MPs within the size ranges from 30 to 500 µm, in fibrous shape, transparent color, and composed of cellophane, PET, PP, and PA polymers, demonstrated enhanced potential for trophic transfer. PHI-based quantitative risk assessment revealed a medium to danger risk of MPs to marine fishery organisms, with hazard indices spanning medium to danger risk levels. These findings provide new insights into the bioaccumulation and risk of MPs across different trophic levels in marine organisms.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138367"},"PeriodicalIF":12.2,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-21DOI: 10.1016/j.jhazmat.2025.138360
Xiaowei Li , Xin Wu , Junzhe Zhang , Changjian Xie , Yingjun Song , Yunpeng Liu , Lingna Zheng , Shujing Zhang , Peng Zhang , Martina G. Vijver , Willie J.G.M. Peijnenburg , Iseult Lynch , Zhiling Guo
This study aims to investigate the toxicity and underlying mechanisms of ZnO nanoparticles (ZnO NPs), bulk ZnO (ZnO MPs), and zinc ions (Zn2 +) on Dugesia japonica planarians, with a focus on their bioaccumulation, transformation, and associated biological effects. Using advanced techniques such as synchrotron X-ray fluorescence (XRF), X-ray Absorption Near Edge Structure (XANES) and single particle ICP-MS (sp-ICP-MS), we measured the accumulation, distribution, and transformation of these materials in planarians. All treatments caused significant Zn accumulation: ZnO NPs increased Zn by 120-fold, ZnO MPs by 100-fold, and Zn2+ by 430-fold. XANES and sp-ICP-MS analysis confirmed that ZnO NPs remained largely in particulate form (40–60 %) following uptake by planarians. Toxicity tests revealed that all treatments impaired blastema growth, locomotion, stem cell proliferation, differentiation, and neural regeneration. ZnO MPs exhibited higher toxicity than ZnO NPs, while Zn2+ resulted in elevated oxidative stress. ZnO NPs induced severe energy damage and triggered cell apoptosis, whereas ZnO MPs caused more pronounced necrosis cell death. Transcriptomic and proteomic analyses showed that all treatments disrupted pathways related to oxidative stress response, energy metabolism and cell apoptosis. ZnO NPs primarily affected the membrane integrity pathway, ZnO MPs altered cell homeostasis and membrane potential, while Zn2+ exposure triggered metal ion-specific cellular reactions. These molecular and cellular changes collectively explain the observed phenotypic outcomes, which align with the Adverse Outcome Pathway framework. The findings provide insights into the environmental risks of different ZnO forms and highlight their distinct toxicity mechanisms.
{"title":"Key events relating to homeostasis and regeneration of freshwater planarians (Dugesia Japonica) after exposure to various ZnO-forms","authors":"Xiaowei Li , Xin Wu , Junzhe Zhang , Changjian Xie , Yingjun Song , Yunpeng Liu , Lingna Zheng , Shujing Zhang , Peng Zhang , Martina G. Vijver , Willie J.G.M. Peijnenburg , Iseult Lynch , Zhiling Guo","doi":"10.1016/j.jhazmat.2025.138360","DOIUrl":"10.1016/j.jhazmat.2025.138360","url":null,"abstract":"<div><div>This study aims to investigate the toxicity and underlying mechanisms of ZnO nanoparticles (ZnO NPs), bulk ZnO (ZnO MPs), and zinc ions (Zn<sup>2 +</sup>) on <em>Dugesia japonica</em> planarians, with a focus on their bioaccumulation, transformation, and associated biological effects. Using advanced techniques such as synchrotron X-ray fluorescence (XRF), X-ray Absorption Near Edge Structure (XANES) and single particle ICP-MS (sp-ICP-MS), we measured the accumulation, distribution, and transformation of these materials in planarians. All treatments caused significant Zn accumulation: ZnO NPs increased Zn by 120-fold, ZnO MPs by 100-fold, and Zn<sup>2+</sup> by 430-fold. XANES and sp-ICP-MS analysis confirmed that ZnO NPs remained largely in particulate form (40–60 %) following uptake by planarians. Toxicity tests revealed that all treatments impaired blastema growth, locomotion, stem cell proliferation, differentiation, and neural regeneration. ZnO MPs exhibited higher toxicity than ZnO NPs, while Zn<sup>2+</sup> resulted in elevated oxidative stress. ZnO NPs induced severe energy damage and triggered cell apoptosis, whereas ZnO MPs caused more pronounced necrosis cell death. Transcriptomic and proteomic analyses showed that all treatments disrupted pathways related to oxidative stress response, energy metabolism and cell apoptosis. ZnO NPs primarily affected the membrane integrity pathway, ZnO MPs altered cell homeostasis and membrane potential, while Zn<sup>2+</sup> exposure triggered metal ion-specific cellular reactions. These molecular and cellular changes collectively explain the observed phenotypic outcomes, which align with the Adverse Outcome Pathway framework. The findings provide insights into the environmental risks of different ZnO forms and highlight their distinct toxicity mechanisms.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138360"},"PeriodicalIF":12.2,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-21DOI: 10.1016/j.jhazmat.2025.138365
Yun-Seo Lee , Jae Jun Lee , Sooseong Lee , Jiyun Kang , Ki-Tae Kim , Cheal Kim
Microplastics (MPs) are recognized as a significant environmental hazardous material. Therefore, identifying and tracking microplastics are crucial to understand their harmfulness. Herein, we introduce (Z)-N'-(pyren-1-ylmethylene)pyrazine-2-carbohydrazide (PPC) as a cost-effective and efficient fluorescence staining agent. Unique properties of PPC, including aggregation-induced emission (AIE) and solvatochromism, were verified by spectroscopic studies and theoretical calculations. PPC selectively stained eight types of MPs with green fluorescence in water/tetrahydrofuran (THF) solution (97/3, v/v). Interestingly, only polyurethane (PU) was selectively stained both in green and blue channel using 50 % ethanol (EtOH) treatment. Moreover, with 30 % EtOH treatment, PU and polyethylene terephthalate (PET) were distinctly stained in the blue channel, highlighting their selective fluorescence. These results suggested that changes in solvent polarity induced by different EtOH amounts might alter the binding strength between PPC and MPs, resulting in varying fluorescence responses. In addition, the adsorption interaction of PPC to MPs was proposed, based on thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), and non-covalent interaction (NCI) analysis. PPC could identify MPs without any influence in the size, aging and pH difference. Successfully, PPC could stain eight types of MPs and selectively PU and PET in artificial environmental samples like seawater, river water and soil. Moreover, PPC-labeled MPs could be tracked in biological system such as Danio rerio (zebrafish) to monitor the transit and accumulation of MPs. These results underscore the potential of PPC for efficient detection of MPs in environmental and biological systems.
{"title":"A cost-effective and efficient fluorescence staining agent for the identification of microplastics in environmental samples and zebrafish (Danio rerio)","authors":"Yun-Seo Lee , Jae Jun Lee , Sooseong Lee , Jiyun Kang , Ki-Tae Kim , Cheal Kim","doi":"10.1016/j.jhazmat.2025.138365","DOIUrl":"10.1016/j.jhazmat.2025.138365","url":null,"abstract":"<div><div>Microplastics (MPs) are recognized as a significant environmental hazardous material. Therefore, identifying and tracking microplastics are crucial to understand their harmfulness. Herein, we introduce (<em>Z</em>)-<em>N'</em>-(pyren-1-ylmethylene)pyrazine-2-carbohydrazide (PPC) as a cost-effective and efficient fluorescence staining agent. Unique properties of PPC, including aggregation-induced emission (AIE) and solvatochromism, were verified by spectroscopic studies and theoretical calculations. PPC selectively stained eight types of MPs with green fluorescence in water/tetrahydrofuran (THF) solution (97/3, v/v). Interestingly, only polyurethane (PU) was selectively stained both in green and blue channel using 50 % ethanol (EtOH) treatment. Moreover, with 30 % EtOH treatment, PU and polyethylene terephthalate (PET) were distinctly stained in the blue channel, highlighting their selective fluorescence. These results suggested that changes in solvent polarity induced by different EtOH amounts might alter the binding strength between PPC and MPs, resulting in varying fluorescence responses. In addition, the adsorption interaction of PPC to MPs was proposed, based on thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), and non-covalent interaction (NCI) analysis. PPC could identify MPs without any influence in the size, aging and pH difference. Successfully, PPC could stain eight types of MPs and selectively PU and PET in artificial environmental samples like seawater, river water and soil. Moreover, PPC-labeled MPs could be tracked in biological system such as <em>Danio rerio</em> (zebrafish) to monitor the transit and accumulation of MPs. These results underscore the potential of PPC for efficient detection of MPs in environmental and biological systems.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138365"},"PeriodicalIF":12.2,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-21DOI: 10.1016/j.jhazmat.2025.138350
Xiaofang Li , Bikram Basak , Rahul S. Tanpure , Xin Zheng , Byong-Hun Jeon
Microbial metal resistance, a trait that enables microorganisms to withstand high levels of toxic metals, has been studied for over a century. The significance of uncovering these mechanisms goes beyond basic science as they have implications for human health through their connection to microbial pathogenesis, metal bioremediation, and biomining. Recent advances in analytical chemistry and molecular biology have accelerated the discovery and understanding of genetic mechanisms underlying microbial metal resistance, identifying specific metal resistance genes and their operons. The emergence of omics tools has further propelled research towards a comprehensive understanding of how cells respond to metal stress at the systemic level, revealing the complex regulatory networks and evolutionary dynamics that drive microbial adaptation to metal-rich environments. In this article, we present a historical overview of the evolving understanding of the genetic determinants of metal resistance in microbes. Through multiple narrative threads, we illustrate how our knowledge of microbial metal resistance and genetics has interacted with genetic tools and concept development. This review also discusses how our understanding of microbial metal resistance has progressed from the Mendelian perspective to the current systems biology viewpoint, particularly as omics approaches have considerably enhanced our understanding. This system-level understanding has opened new possibilities for genetically engineered microorganisms to regulate metal homeostasis.
{"title":"Unraveling the genetic basis of microbial metal resistance: shift from mendelian to systems biology","authors":"Xiaofang Li , Bikram Basak , Rahul S. Tanpure , Xin Zheng , Byong-Hun Jeon","doi":"10.1016/j.jhazmat.2025.138350","DOIUrl":"10.1016/j.jhazmat.2025.138350","url":null,"abstract":"<div><div>Microbial metal resistance, a trait that enables microorganisms to withstand high levels of toxic metals, has been studied for over a century. The significance of uncovering these mechanisms goes beyond basic science as they have implications for human health through their connection to microbial pathogenesis, metal bioremediation, and biomining. Recent advances in analytical chemistry and molecular biology have accelerated the discovery and understanding of genetic mechanisms underlying microbial metal resistance, identifying specific metal resistance genes and their operons. The emergence of omics tools has further propelled research towards a comprehensive understanding of how cells respond to metal stress at the systemic level, revealing the complex regulatory networks and evolutionary dynamics that drive microbial adaptation to metal-rich environments. In this article, we present a historical overview of the evolving understanding of the genetic determinants of metal resistance in microbes. Through multiple narrative threads, we illustrate how our knowledge of microbial metal resistance and genetics has interacted with genetic tools and concept development. This review also discusses how our understanding of microbial metal resistance has progressed from the Mendelian perspective to the current systems biology viewpoint, particularly as omics approaches have considerably enhanced our understanding. This system-level understanding has opened new possibilities for genetically engineered microorganisms to regulate metal homeostasis.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138350"},"PeriodicalIF":12.2,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-20DOI: 10.1016/j.jhazmat.2025.138344
Xuexia Gao , Binbin Li , Xiaohan Yuan , Yajing Yang , Min Lv , Zhishang Zhu , Jinfeng Song , Chengbo Gu
Phytoremediation associated with plant-beneficial bacteria has gained considerable attention for its efficiency in remediating cadmium (Cd)-contaminated soil. In this study, we isolated an endophytic bacterium, PEB-9, from pigeon pea, which was identified as Bacillus cereus and exhibited strong plant growth-promoting traits along with high Cd resistance in vitro. Pot experiments demonstrated that PEB-9 inoculation increased in pigeon pea biomass (11.45–19.29 %), Cd accumulation (43.89–69.90 %), and the Cd transfer factor (4.17–16.89 %) in Cd-stress soil (2–10 mg/kg), with soil remediation efficiency improving by 11.1–15.4 %. Under Cd stress, PEB-9-treated pigeon pea exhibited significant improvement in the activities of superoxide dismutase, peroxidase, and catalase, while a notable decrease in malondialdehyde content, indicating a reduction in Cd cytotoxicity. Additionally, the chlorophyll content in PEB-9-treated plants was significantly higher than that in the control group. Furthermore, PEB-9 inoculation enhanced bioavailable Cd, soil enzymes activity and nutrient content, including available nitrogen, phosphorus, and organic matter, while also boosting the relative abundance of stress-resistant bacterial groups, such as Proteobacteria and Actinobacteria. Correlation analysis indicated that soil nutrient changes induced by PEB-9 significantly influenced bacterial community structure, thereby regulating plant physiological responses, and improving Cd remediation efficacy of pigeon pea. These findings offer a valuable basis for the practical implementation of PEB-9 in remediating Cd-contaminated soils.
{"title":"Potential of pigeon pea [Cajanus cajan (L.) Millsp.] associated with endophytic bacterium Bacillus cereus PEB-9 to remediate cadmium-contaminated soil","authors":"Xuexia Gao , Binbin Li , Xiaohan Yuan , Yajing Yang , Min Lv , Zhishang Zhu , Jinfeng Song , Chengbo Gu","doi":"10.1016/j.jhazmat.2025.138344","DOIUrl":"10.1016/j.jhazmat.2025.138344","url":null,"abstract":"<div><div>Phytoremediation associated with plant-beneficial bacteria has gained considerable attention for its efficiency in remediating cadmium (Cd)-contaminated soil. In this study, we isolated an endophytic bacterium, PEB-9, from pigeon pea, which was identified as <em>Bacillus cereus</em> and exhibited strong plant growth-promoting traits along with high Cd resistance <em>in vitro</em>. Pot experiments demonstrated that PEB-9 inoculation increased in pigeon pea biomass (11.45–19.29 %), Cd accumulation (43.89–69.90 %), and the Cd transfer factor (4.17–16.89 %) in Cd-stress soil (2–10 mg/kg), with soil remediation efficiency improving by 11.1–15.4 %. Under Cd stress, PEB-9-treated pigeon pea exhibited significant improvement in the activities of superoxide dismutase, peroxidase, and catalase, while a notable decrease in malondialdehyde content, indicating a reduction in Cd cytotoxicity. Additionally, the chlorophyll content in PEB-9-treated plants was significantly higher than that in the control group. Furthermore, PEB-9 inoculation enhanced bioavailable Cd, soil enzymes activity and nutrient content, including available nitrogen, phosphorus, and organic matter, while also boosting the relative abundance of stress-resistant bacterial groups, such as <em>Proteobacteria</em> and <em>Actinobacteria</em>. Correlation analysis indicated that soil nutrient changes induced by PEB-9 significantly influenced bacterial community structure, thereby regulating plant physiological responses, and improving Cd remediation efficacy of pigeon pea. These findings offer a valuable basis for the practical implementation of PEB-9 in remediating Cd-contaminated soils.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138344"},"PeriodicalIF":12.2,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-20DOI: 10.1016/j.jhazmat.2025.138352
Shuaiqi Wang , Bing Zhang , Sainan Zhang , Shaohui Yang , Meng-Zhu Lu , Jiehua Wang
Small organic compounds (SOCs) are widespread environmental pollutants that pose a significant threat to ecosystem health and human well-being. In this study, the FrmA gene from Escherichia coli was overexpressed alone or in combination with FrmB in Arabidopsis thaliana and their resistance to multiple SOCs was investigated. The transgenic plants exhibited varying degrees of increased tolerance to methanol, formic acid, toluene, and phenol, extending beyond the known role of FrmA in formaldehyde metabolism. Biochemical and histochemical analyses showed reduced oxidative damage, especially in the FrmA/BOE lines, as evidenced by lower malondialdehyde (MDA), H2O2 and O2•− levels, indicating improved scavenging of reactive oxygen species (ROS). SOC treatment led to significantly higher levels of glutathione (GSH) and, to a lesser extent, ascorbic acid (AsA) in the transgenic plants than in the wild-types. After methanol exposure, GSH levels increased by 95 % and 72 % in the FrmA/BOE and FrmAOE plants, respectively, while showing no significant increase in the wild-type plants. The transgenic plants also maintained higher GSH:GSSG and AsA:DHA ratios, exhibited upregulated glutathione reductase (GR) and dehydroascorbate reductase (DHAR) activities, and correspondingly increased gene expression. In addition, the photosynthetic parameters of the transgenic plants were less affected by SOC stress, which represents a significant photosynthetic advantage. These results emphasize the potential of genetically engineered plants for phytoremediation and crop improvement, as they exhibit increased tolerance to multiple hazardous SOCs. This research lays the foundation for sustainable approaches to combat pollution and improve plant resilience in the face of escalating environmental problems.
{"title":"The overexpression of E. coli formaldehyde metabolism genes in Arabidopsis conferred varying degrees of resistance to oxidative stress induced by small organic compounds","authors":"Shuaiqi Wang , Bing Zhang , Sainan Zhang , Shaohui Yang , Meng-Zhu Lu , Jiehua Wang","doi":"10.1016/j.jhazmat.2025.138352","DOIUrl":"10.1016/j.jhazmat.2025.138352","url":null,"abstract":"<div><div>Small organic compounds (SOCs) are widespread environmental pollutants that pose a significant threat to ecosystem health and human well-being. In this study, the <em>FrmA</em> gene from <em>Escherichia coli</em> was overexpressed alone or in combination with <em>FrmB</em> in <em>Arabidopsis thaliana</em> and their resistance to multiple SOCs was investigated. The transgenic plants exhibited varying degrees of increased tolerance to methanol, formic acid, toluene, and phenol, extending beyond the known role of <em>FrmA</em> in formaldehyde metabolism. Biochemical and histochemical analyses showed reduced oxidative damage, especially in the <em>FrmA/B</em><sup>OE</sup> lines, as evidenced by lower malondialdehyde (MDA), H<sub>2</sub>O<sub>2</sub> and O<sub>2</sub><sup>•−</sup> levels, indicating improved scavenging of reactive oxygen species (ROS). SOC treatment led to significantly higher levels of glutathione (GSH) and, to a lesser extent, ascorbic acid (AsA) in the transgenic plants than in the wild-types. After methanol exposure, GSH levels increased by 95 % and 72 % in the <em>FrmA/B</em><sup>OE</sup> and <em>FrmA</em><sup>OE</sup> plants, respectively, while showing no significant increase in the wild-type plants. The transgenic plants also maintained higher GSH:GSSG and AsA:DHA ratios, exhibited upregulated glutathione reductase (GR) and dehydroascorbate reductase (DHAR) activities, and correspondingly increased gene expression. In addition, the photosynthetic parameters of the transgenic plants were less affected by SOC stress, which represents a significant photosynthetic advantage. These results emphasize the potential of genetically engineered plants for phytoremediation and crop improvement, as they exhibit increased tolerance to multiple hazardous SOCs. This research lays the foundation for sustainable approaches to combat pollution and improve plant resilience in the face of escalating environmental problems.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"493 ","pages":"Article 138352"},"PeriodicalIF":12.2,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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