Pub Date : 2025-01-15DOI: 10.1016/j.jhazmat.2025.137233
Qinyu Wang, Xuewen Peng, Niu Feng, Yiping Chen, Chunhua Deng
Artificial intelligence-assisted imaging biosensors have attracted increasing attention due to their flexibility, allowing for the digital image analysis and quantification of biomarkers. While deep learning methods have led to advancements in biomarker identification, the diversity in the density and adherence of targets still poses a serious challenge. In this regard, we propose CellNet, a neural network model specifically designed for detecting dense targets. The model uses a shape-aware radial basis function to learn the kernel representation of objects, improving the target counting accuracy, and exhibits excellent performance in identifying adherent polystyrene microspheres, with a detection accuracy of 98.39%. Considering these factors, we developed a biotin–streptavidin-based biosensing method using artificial intelligence transcoding (bs-SMART) to detect procalcitonin in serum samples. Given its excellent accuracy and sensitivity (limit of detection = 8.5 pg/mL), the technique provides a reliable platform for the accurate diagnosis of diseases. Furthermore, this study validated the ability of CellNet to recognize irregular and adherent cells. Overall, CellNet not only contributes to advancing computer vision and image processing technology but also presents potential benefits for medical diagnostics, food safety testing, and environmental monitoring.
{"title":"Kernel Representation-based End-to-End Network-enabled Decoding Strategy for Precise and Medical Diagnosis","authors":"Qinyu Wang, Xuewen Peng, Niu Feng, Yiping Chen, Chunhua Deng","doi":"10.1016/j.jhazmat.2025.137233","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137233","url":null,"abstract":"Artificial intelligence-assisted imaging biosensors have attracted increasing attention due to their flexibility, allowing for the digital image analysis and quantification of biomarkers. While deep learning methods have led to advancements in biomarker identification, the diversity in the density and adherence of targets still poses a serious challenge. In this regard, we propose CellNet, a neural network model specifically designed for detecting dense targets. The model uses a shape-aware radial basis function to learn the kernel representation of objects, improving the target counting accuracy, and exhibits excellent performance in identifying adherent polystyrene microspheres, with a detection accuracy of 98.39%. Considering these factors, we developed a biotin–streptavidin-based biosensing method using artificial intelligence transcoding (bs-SMART) to detect procalcitonin in serum samples. Given its excellent accuracy and sensitivity (limit of detection = 8.5<!-- --> <!-- -->pg/mL), the technique provides a reliable platform for the accurate diagnosis of diseases. Furthermore, this study validated the ability of CellNet to recognize irregular and adherent cells. Overall, CellNet not only contributes to advancing computer vision and image processing technology but also presents potential benefits for medical diagnostics, food safety testing, and environmental monitoring.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"20 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142981589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-15DOI: 10.1016/j.jhazmat.2025.137203
Zhuo Chen, Qiwei Chen, Yan Wang, Wuwei Zou, Yuan Li, Jinhan Mo
The primary challenges impeding the extensive application of adsorption for indoor air purification have been low efficiency and effective capacity. To fill the research gap, we developed carbonaceous net-like adsorption films featuring multi-scale porous structures for efficient indoor formaldehyde removal. By optimizing the interfacial mass transfer and internal diffusion, we designed macro- to mesoscale meshes on the film surface and micro- to nano-scale pores within the materials, which were achieved by direct-ink-writing (DIW) printing and sacrificial template methods, respectively. Compared to unmodified planar films, the developed films exhibited a significant increase in the initial single-pass efficiency of formaldehyde from 68.1% to 89.0%, with the 8-hour effective adsorption capacity (EACad) spiking from 2.74 mg/g to 8.60 mg/g. Through thermal regeneration, the film demonstrated stable operation for 30 days through a long-term experiment over 250 hours, and EACad significantly increased to 241.7 mg/g. The multi-scale adsorption films achieved nearly the highest purification rates and capacities among existing physisorption and chemisorption technologies, with the lowest energy cost of 0.37 kW·h per day. The innovative design and fabrication of multi-scale adsorption films evidence its substantial application for indoor formaldehyde purification and provide a viable solution for carbon capture and gas separation in environmental engineering.
{"title":"Tuning multi-scale pore structures in carbonaceous films via direct ink writing and sacrificial templates for efficient indoor formaldehyde removal","authors":"Zhuo Chen, Qiwei Chen, Yan Wang, Wuwei Zou, Yuan Li, Jinhan Mo","doi":"10.1016/j.jhazmat.2025.137203","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137203","url":null,"abstract":"The primary challenges impeding the extensive application of adsorption for indoor air purification have been low efficiency and effective capacity. To fill the research gap, we developed carbonaceous net-like adsorption films featuring multi-scale porous structures for efficient indoor formaldehyde removal. By optimizing the interfacial mass transfer and internal diffusion, we designed macro- to mesoscale meshes on the film surface and micro- to nano-scale pores within the materials, which were achieved by direct-ink-writing (DIW) printing and sacrificial template methods, respectively. Compared to unmodified planar films, the developed films exhibited a significant increase in the initial single-pass efficiency of formaldehyde from 68.1% to 89.0%, with the 8-hour effective adsorption capacity (<em>EAC</em><sub>ad</sub>) spiking from 2.74<!-- --> <!-- -->mg/g to 8.60<!-- --> <!-- -->mg/g. Through thermal regeneration, the film demonstrated stable operation for 30 days through a long-term experiment over 250<!-- --> <!-- -->hours, and <em>EAC</em><sub>ad</sub> significantly increased to 241.7<!-- --> <!-- -->mg/g. The multi-scale adsorption films achieved nearly the highest purification rates and capacities among existing physisorption and chemisorption technologies, with the lowest energy cost of 0.37<!-- --> <!-- -->kW·h per day. The innovative design and fabrication of multi-scale adsorption films evidence its substantial application for indoor formaldehyde purification and provide a viable solution for carbon capture and gas separation in environmental engineering.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"10 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142987713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
How plastics coupled with metals regulate microbial functions-diversity relationships remain unknown in plateau soil environment. Three representative catchments in the Qinghai-Tibet Plateau, focusing on microplastics, their plasticisers, and metals in soils, were investigated. This research explores responses of bacterial diversity and functions to the co-existence of target pollutants, and pathways by which target pollutants regulate the diversity. Soil bacterial beta diversity and functional genes exhibited negative correlations with phthalate esters across three catchments (p < 0.05). Dibutyl phthalate emerged as a primary factor affecting beta diversity, rather than the quantity of microplastics. Additionally, the synergy of cadmium and fiber-shaped microplastics exacerbated the impact on diversity. Structural equation modeling further elucidated that plastics, copper, and iron influenced nirK/nirS genes and phoD gene, subsequently affected cbbL/cbbM genes, and ultimately the diversity. In this context, microplastics, phthalate esters and copper, iron exerted antagonistic effects on one another. Consequently, the co-existence of plastics and cadmium weakened soil bacterial diversity in the Qinghai-Tibet Plateau by disrupting bacterial functions, but micronutrients alleviated these negative impacts. This research reveals that the co-existence of plastics and metals regulates soil bacterial diversity in the Qinghai-Tibet Plateau, providing a valuable reference for the protection of microbial ecology in plateau regions.
{"title":"Synergy of plastics and heavy metals weakened soil bacterial diversity by regulating microbial functions in the Qinghai-Tibet Plateau","authors":"Yonglu Wang, Fengsong Zhang, Lucun Yang, Guixiang Zhang, Huaxin Wang, Shiliang Zhu, Hongyu Zhang, Tingyu Guo","doi":"10.1016/j.jhazmat.2025.137241","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137241","url":null,"abstract":"How plastics coupled with metals regulate microbial functions-diversity relationships remain unknown in plateau soil environment. Three representative catchments in the Qinghai-Tibet Plateau, focusing on microplastics, their plasticisers, and metals in soils, were investigated. This research explores responses of bacterial diversity and functions to the co-existence of target pollutants, and pathways by which target pollutants regulate the diversity. Soil bacterial beta diversity and functional genes exhibited negative correlations with phthalate esters across three catchments (<em>p</em> < 0.05). Dibutyl phthalate emerged as a primary factor affecting beta diversity, rather than the quantity of microplastics. Additionally, the synergy of cadmium and fiber-shaped microplastics exacerbated the impact on diversity. Structural equation modeling further elucidated that plastics, copper, and iron influenced <em>nirK</em>/<em>nirS</em> genes and <em>phoD</em> gene, subsequently affected <em>cbbL</em>/<em>cbbM</em> genes, and ultimately the diversity. In this context, microplastics, phthalate esters and copper, iron exerted antagonistic effects on one another. Consequently, the co-existence of plastics and cadmium weakened soil bacterial diversity in the Qinghai-Tibet Plateau by disrupting bacterial functions, but micronutrients alleviated these negative impacts. This research reveals that the co-existence of plastics and metals regulates soil bacterial diversity in the Qinghai-Tibet Plateau, providing a valuable reference for the protection of microbial ecology in plateau regions.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"1 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142981597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-15DOI: 10.1016/j.jhazmat.2025.137193
Li Ma, Hao Wang, Yuqiong Guo, Liyao Qin, Lingyu Ren, Tingting Ku, Guangke Li, Nan Sang
Fine particulate matter (PM2.5) is one of the most concerning air pollutants, with emerging evidence indicating that it can negatively impact embryonic development and lead to adverse birth outcomes. Hematopoiesis is a critical process essential for the survival and normal development of the embryo, consisting of three temporally overlapping stages and involving multiple hematopoietic loci, including the yolk sac and fetal liver. Therefore, we hypothesized that abnormal embryonic hematopoietic development can significantly influence developmental outcomes. In this study, we established a prenatal PM2.5 exposure model and observed decreased embryo weights and elevated platelet counts at embryonic day 18.5 (E18.5). Additionally, we employed flow cytometry and colony-forming unit assays, which revealed a significant decrease in the proliferative differentiation potential of erythro-myeloid progenitors in the E10.5 yolk sac, as well as a reduction in both the number and function of hematopoietic stem progenitor cells in the E14.5 fetal liver. Through bioinformatic analysis, we identified that these alterations are associated with several typical biological processes and genes regarding cell proliferation, cell differentiation, response to hypoxia, and regulation of hematopoiesis. Importantly, via quantitative real-time PCR, chromatin immunoprecipitation, and immunofluorescence, we further elucidated that prenatal exposure to PM2.5 affects embryonic hematopoiesis by downregulating the expression of SOX2, an important transcription factor involved in embryonic development, along with its related genes. Collectively, these findings provide experimental evidence supporting the need for controlling regional PM2.5 exposure to promote child well-being in polluted areas.
{"title":"Prenatal PM2.5 exposure affects embryonic hematopoietic development through SOX2-regulated gene expression","authors":"Li Ma, Hao Wang, Yuqiong Guo, Liyao Qin, Lingyu Ren, Tingting Ku, Guangke Li, Nan Sang","doi":"10.1016/j.jhazmat.2025.137193","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137193","url":null,"abstract":"Fine particulate matter (PM<sub>2.5</sub>) is one of the most concerning air pollutants, with emerging evidence indicating that it can negatively impact embryonic development and lead to adverse birth outcomes. Hematopoiesis is a critical process essential for the survival and normal development of the embryo, consisting of three temporally overlapping stages and involving multiple hematopoietic loci, including the yolk sac and fetal liver. Therefore, we hypothesized that abnormal embryonic hematopoietic development can significantly influence developmental outcomes. In this study, we established a prenatal PM<sub>2.5</sub> exposure model and observed decreased embryo weights and elevated platelet counts at embryonic day 18.5 (E18.5). Additionally, we employed flow cytometry and colony-forming unit assays, which revealed a significant decrease in the proliferative differentiation potential of erythro-myeloid progenitors in the E10.5 yolk sac, as well as a reduction in both the number and function of hematopoietic stem progenitor cells in the E14.5 fetal liver. Through bioinformatic analysis, we identified that these alterations are associated with several typical biological processes and genes regarding cell proliferation, cell differentiation, response to hypoxia, and regulation of hematopoiesis. Importantly, via quantitative real-time PCR, chromatin immunoprecipitation, and immunofluorescence, we further elucidated that prenatal exposure to PM<sub>2.5</sub> affects embryonic hematopoiesis by downregulating the expression of SOX2, an important transcription factor involved in embryonic development, along with its related genes. Collectively, these findings provide experimental evidence supporting the need for controlling regional PM<sub>2.5</sub> exposure to promote child well-being in polluted areas.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"8 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142987709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The ecological impact of tire wear particles (TWP), a significant source of microplastics pollution, is increasingly concerning, especially given their potential effects on the health of aquatic ecosystems. This study investigates the size-dependent ecotoxicological responses of zebrafish (Danio rerio) to TWP exposure, focusing on physiological, metabolic, and microbial community impacts over a 15-day exposure period followed by a 15-day excretion period. Through integrated analysis of gut microbiome composition, liver transcriptomics, and host physiological markers, we found that smaller TWP particles (< 120 μm) induced oxidative stress, evidenced by increased SOD and MDA levels, and inhibited growth by reducing body mass and gut length. In contrast, larger TWP particles (250 - 380 μm) caused more substantial disruptions in lipid and xenobiotic metabolic pathways, as shown by significant downregulation of key metabolic genes (acads, cpt2_1, hadhaa), and alterations in the gut microbiome, including the enrichment of pathogenic genera, such as Enterococcus and Fusobacterium, while depleting beneficial microbes like Acinetobacter and Methyloversatilis. These microbiome shifts led to a more complex and potentially pathogenic gut microbiome. Notably, zebrafish displayed adaptive resilience during the excretion period, with significant recovery in body mass and microbial composition, emphasizing the adaptive capacity of aquatic organisms to pollutants. Our findings underscore the broader ecological risks posed by TWP, the pivotal role of gut microbiota in host resilience to pollutants, and the need for comprehensive management strategies addressing emerging contaminants in aquatic ecosystems.
{"title":"Size-dependent ecotoxicological impacts of tire wear particles on zebrafish physiology and gut microbiota: implications for aquatic ecosystem health","authors":"Yun Zhang, Qianqian Song, Qingxuan Meng, Tianyu Zhao, Xiaolong Wang, Xinrui Meng, Jing Cong","doi":"10.1016/j.jhazmat.2025.137215","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137215","url":null,"abstract":"The ecological impact of tire wear particles (TWP), a significant source of microplastics pollution, is increasingly concerning, especially given their potential effects on the health of aquatic ecosystems. This study investigates the size-dependent ecotoxicological responses of zebrafish (<em>Danio rerio</em>) to TWP exposure, focusing on physiological, metabolic, and microbial community impacts over a 15-day exposure period followed by a 15-day excretion period. Through integrated analysis of gut microbiome composition, liver transcriptomics, and host physiological markers, we found that smaller TWP particles (< 120 μm) induced oxidative stress, evidenced by increased SOD and MDA levels, and inhibited growth by reducing body mass and gut length. In contrast, larger TWP particles (250 - 380 μm) caused more substantial disruptions in lipid and xenobiotic metabolic pathways, as shown by significant downregulation of key metabolic genes (<em>acads</em>, <em>cpt2_1</em>, <em>hadhaa</em>), and alterations in the gut microbiome, including the enrichment of pathogenic genera, such as <em>Enterococcus</em> and <em>Fusobacterium</em>, while depleting beneficial microbes like <em>Acinetobacter</em> and <em>Methyloversatilis</em>. These microbiome shifts led to a more complex and potentially pathogenic gut microbiome. Notably, zebrafish displayed adaptive resilience during the excretion period, with significant recovery in body mass and microbial composition, emphasizing the adaptive capacity of aquatic organisms to pollutants. Our findings underscore the broader ecological risks posed by TWP, the pivotal role of gut microbiota in host resilience to pollutants, and the need for comprehensive management strategies addressing emerging contaminants in aquatic ecosystems.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"118 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142987712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tinidazole (TNZ), a common nitroimidazole antibiotic, is pervasive in aquatic ecosystems, posing potential threats to marine organisms. The environmental fate of TNZ, particularly under solar irradiation, and the associated secondary risks are not well characterized. Herein, the photochemical reactivity of TNZ and four other typical nitroimidazoles (i.e., metronidazole, ornidazole, dimetridazole, and secnidazole) were quantified for multiple photoreactive species. The photolysis products of these nitroimidazoles were identified under solar irradiation, from which the reaction pathways were tentatively proposed. Furthermore, the photo-induced toxicity evolution mechanisms of TNZ were investigated by comparing phenotypic, transcriptomic, and metabolomic changes in marine medaka embryos (Oryzias melastigma) after exposure to TNZ and its photo-irradiated mixtures. Our results indicated that the photo-irradiated TNZ enhanced visual toxicity to marine medaka embryos compared to the parent compound. The photolysis mixtures induced embryonic ocular malformation and significantly affected the expression of the associated genes with the initiation/termination of the phototransduction cascade, leading to metabolite changes related to visual impairment. This work reported the first comprehensive assessment of the photolysis-mediated environmental fate and secondary risks of TNZ in seawater. The findings highlighted the necessity of including complex photolysis mixtures under solar irradiation in future chemical risk assessments of marine environments.
{"title":"Sunlight-mediated environmental risks of tinidazole in seawater: A neglected ocular toxicity of photolysis mixtures","authors":"Ruping Zheng, Shengqi Zhang, Shengyue Chen, Wenqi Zha, Xinyue Li, Qiuru Li, Jinlin He, Shanshan He, Mingbao Feng, Yingjia Shen","doi":"10.1016/j.jhazmat.2025.137217","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137217","url":null,"abstract":"Tinidazole (TNZ), a common nitroimidazole antibiotic, is pervasive in aquatic ecosystems, posing potential threats to marine organisms. The environmental fate of TNZ, particularly under solar irradiation, and the associated secondary risks are not well characterized. Herein, the photochemical reactivity of TNZ and four other typical nitroimidazoles (i.e., metronidazole, ornidazole, dimetridazole, and secnidazole) were quantified for multiple photoreactive species. The photolysis products of these nitroimidazoles were identified under solar irradiation, from which the reaction pathways were tentatively proposed. Furthermore, the photo-induced toxicity evolution mechanisms of TNZ were investigated by comparing phenotypic, transcriptomic, and metabolomic changes in marine medaka embryos (<em>Oryzias melastigma</em>) after exposure to TNZ and its photo-irradiated mixtures. Our results indicated that the photo-irradiated TNZ enhanced visual toxicity to marine medaka embryos compared to the parent compound. The photolysis mixtures induced embryonic ocular malformation and significantly affected the expression of the associated genes with the initiation/termination of the phototransduction cascade, leading to metabolite changes related to visual impairment. This work reported the first comprehensive assessment of the photolysis-mediated environmental fate and secondary risks of TNZ in seawater. The findings highlighted the necessity of including complex photolysis mixtures under solar irradiation in future chemical risk assessments of marine environments.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"42 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142974871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-14DOI: 10.1016/j.jhazmat.2025.137201
A. Ouaksel, A. Carboni, D. Slomberg, V. Vidal, O. Proux, C. Santaella, L. Brousset, B. Angeletti, A. Thiéry, J. Rose, M. Auffan
Within the ITER project (International Thermonuclear Experimental Reactor) an international project building a magnetic confinement device to achieve fusion as a sustainable energy source, tungsten (W) is planned to serve as a plasma-facing component (PFC) in the tokamak, a magnetic confinement device used to produce controlled thermonuclear fusion power. Post plasma-W interactions, submicron tungsten particles can be released. This study investigated the exposure of lentic freshwater ecosystems to ITER-like tungsten nanoparticles in indoor aquatic mesocosms. Monitoring included tungsten (bio)distribution, (bio)transformation, speciation, and impacts following a relevant exposure scenario (chronic, medium-term, low-dose contamination). Additionally, mechanistic studies using a combination of microfluidic cells and X-ray Absorption Spectroscopy (XAS) provided a time-resolved understanding of tungsten's oxidative dissolution in freshwater. Following contamination, tungsten persisted in the water column (over 90%), showing significant (~40%) and rapid (< 7 days) oxidation-dissolution and polymerization. This led to significant exposure of planktonic niches, strong affinity of polymeric tungsten species for aquatic vegetation, and potential transfer to higher trophic levels like aquatic snails. Over five weeks, the bio-physicochemical parameters of the mesocosms remained stable, and no acute impacts were observed on micro- and macro-organisms.
Environmental Implication
Tungsten nanoparticles (nanoW) are expected to be released into freshwater environments during the operation and maintenance of ITER (International Thermonuclear Experimental Reactor, a nuclear fusion research and engineering project aimed at creating energy through a fusion process similar to that of the sun). Although nanoW are a hazardous material of concern, there is a significant lack of knowledge regarding their behavior, fate, and toxicity in aquatic ecosystems. The present work investigates this topic by (i) mimicking a realistic exposure scenario in mesocosm, (ii) characterizing the (bio)distribution, fate, and (bio)transformation of tungsten nanoparticles in the environmental compartments, (iii) identifying its exposure pathways at ecological niches level and (iii) providing a time-resolved understanding of the nanoW speciation and oxidative dissolution mechanisms in freshwater environments.
{"title":"Behavior and fate of ITER-like tungsten nanoparticles in freshwater ecosystems produced during operation and maintenance","authors":"A. Ouaksel, A. Carboni, D. Slomberg, V. Vidal, O. Proux, C. Santaella, L. Brousset, B. Angeletti, A. Thiéry, J. Rose, M. Auffan","doi":"10.1016/j.jhazmat.2025.137201","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137201","url":null,"abstract":"Within the ITER project (International Thermonuclear Experimental Reactor) an international project building a magnetic confinement device to achieve fusion as a sustainable energy source, tungsten (W) is planned to serve as a plasma-facing component (PFC) in the tokamak, a magnetic confinement device used to produce controlled thermonuclear fusion power. Post plasma-W interactions, submicron tungsten particles can be released. This study investigated the exposure of lentic freshwater ecosystems to ITER-like tungsten nanoparticles in indoor aquatic mesocosms. Monitoring included tungsten (bio)distribution, (bio)transformation, speciation, and impacts following a relevant exposure scenario (chronic, medium-term, low-dose contamination). Additionally, mechanistic studies using a combination of microfluidic cells and X-ray Absorption Spectroscopy (XAS) provided a time-resolved understanding of tungsten's oxidative dissolution in freshwater. Following contamination, tungsten persisted in the water column (over 90%), showing significant (~40%) and rapid (< 7 days) oxidation-dissolution and polymerization. This led to significant exposure of planktonic niches, strong affinity of polymeric tungsten species for aquatic vegetation, and potential transfer to higher trophic levels like aquatic snails. Over five weeks, the bio-physicochemical parameters of the mesocosms remained stable, and no acute impacts were observed on micro- and macro-organisms.<h3>Environmental Implication</h3>Tungsten nanoparticles (nanoW) are expected to be released into freshwater environments during the operation and maintenance of ITER (International Thermonuclear Experimental Reactor, a nuclear fusion research and engineering project aimed at creating energy through a fusion process similar to that of the sun). Although nanoW are a hazardous material of concern, there is a significant lack of knowledge regarding their behavior, fate, and toxicity in aquatic ecosystems. The present work investigates this topic by (i) mimicking a realistic exposure scenario in mesocosm, (ii) characterizing the (bio)distribution, fate, and (bio)transformation of tungsten nanoparticles in the environmental compartments, (iii) identifying its exposure pathways at ecological niches level and (iii) providing a time-resolved understanding of the nanoW speciation and oxidative dissolution mechanisms in freshwater environments.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"68 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142981639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-14DOI: 10.1016/j.jhazmat.2025.137225
Kai Lyu, Jiameng Li, Yuting Wu, Jana Asselman, Zhou Yang
The concomitant prevalence of toxic cyanobacteria blooms and plastic pollution in aquatic ecosystems is emerging as a pressing global water pollution dilemma. While toxic cyanobacteria and microplastics (MPs) can each independently exert significant impacts on aquatic biota, the magnitude and trajectory of the combined interactions remains rudimentary. In this study, we evaluated how MPs influences cyanobacterial stress on keystone grazer Daphnia, focusing on population, individual, biochemical and toxicogenomic signatures. We found that toxic Microcystis (TM) adversely affected the fitness of Daphnia populations (intrinsic rate of population increase), and these adverse effects were amplified in the presence of MPs. Through detailed observation, it was ascertained that MPs promoted the ingestion of TM, culminating in enhanced microcystin bioaccumulation. Using the Eco-Evo model, we found that there was potential absence of correlation between the MPs toxicity and the effect size of MPs on the TM. Utilizing gene set enrichment analysis (GSEA), we further identified a marked suppression of molecular pathways and entities crucial to individual growth and development in the TM-MPs consortium compared to exposure to TM alone. The present study provides important insights about the influence of MPs on cyanobacteria toxicity and the prediction the risk of harmful algal blooms in aquatic ecosystems.
{"title":"Changes in population fitness and gene co-expression networks reveal the boosted impact of toxic cyanobacteria on Daphnia magna through microplastic exposure","authors":"Kai Lyu, Jiameng Li, Yuting Wu, Jana Asselman, Zhou Yang","doi":"10.1016/j.jhazmat.2025.137225","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137225","url":null,"abstract":"The concomitant prevalence of toxic cyanobacteria blooms and plastic pollution in aquatic ecosystems is emerging as a pressing global water pollution dilemma. While toxic cyanobacteria and microplastics (MPs) can each independently exert significant impacts on aquatic biota, the magnitude and trajectory of the combined interactions remains rudimentary. In this study, we evaluated how MPs influences cyanobacterial stress on keystone grazer <em>Daphnia</em>, focusing on population, individual, biochemical and toxicogenomic signatures. We found that toxic <em>Microcystis</em> (TM) adversely affected the fitness of <em>Daphnia</em> populations (intrinsic rate of population increase), and these adverse effects were amplified in the presence of MPs. Through detailed observation, it was ascertained that MPs promoted the ingestion of TM, culminating in enhanced microcystin bioaccumulation. Using the Eco-Evo model, we found that there was potential absence of correlation between the MPs toxicity and the effect size of MPs on the TM. Utilizing gene set enrichment analysis (GSEA), we further identified a marked suppression of molecular pathways and entities crucial to individual growth and development in the TM-MPs consortium compared to exposure to TM alone. The present study provides important insights about the influence of MPs on cyanobacteria toxicity and the prediction the risk of harmful algal blooms in aquatic ecosystems.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"83 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142974874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-14DOI: 10.1016/j.jhazmat.2025.137222
Qingling Wang, Jinyu Hou, Li Peng, Wuxing Liu, Yongming Luo
Bioaugmentation offers an effective strategy for the bioremediation of petroleum-contaminated soils. However, little is known about petroleum hydrocarbons (PHs) degradation with thermophilic consortium application under high temperature. A microcosm was established to study hydrocarbons degradation, microbial communities and functional genes response using a thermophilic petroleum-degrading consortium HT. The results showed that the consortium HT significantly enhanced PHs degradation, particularly for medium (C16-C21) (87.1%) and long-chain alkanes (C21-C40) (67.2%) within 140 days under high temperature. Colonization of HT in the soil exhibited lagged characteristics, with a substantial increase in bacterial genera originated from the HT after 60 days. Additionally, LEfSe analysis indicated that the biomarkers of HT treatment were mainly from the HT consortium. Moreover, functional analysis revealed genes related to n-alkane degradation (AlkB, P450, LadA), alkane utilization regulator (AraC, TetR, GntR), as well as several thermotolerance genes were significantly increased in HT treatment. Additionally, network analysis demonstrated distinct co-occurrence patterns induced by nutrient addition and exogenous consortium, with the latter strengthening interactions and stability of bacterial networks under high temperature. This study represents pioneering investigation into the effects of exogenous thermophilic consortium on petroleum degradation, bacterial communities, functional genes and ecological interactions in application of petroleum remediation under thermophilic conditions.
{"title":"Dynamic Responses in Bioaugmentation of Petroleum-Contaminated Soils using Thermophilic Degrading Consortium HT: Hydrocarbons, Microbial Communities, and Functional Genes","authors":"Qingling Wang, Jinyu Hou, Li Peng, Wuxing Liu, Yongming Luo","doi":"10.1016/j.jhazmat.2025.137222","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137222","url":null,"abstract":"Bioaugmentation offers an effective strategy for the bioremediation of petroleum-contaminated soils. However, little is known about petroleum hydrocarbons (PHs) degradation with thermophilic consortium application under high temperature. A microcosm was established to study hydrocarbons degradation, microbial communities and functional genes response using a thermophilic petroleum-degrading consortium HT. The results showed that the consortium HT significantly enhanced PHs degradation, particularly for medium (C16-C21) (87.1%) and long-chain alkanes (C21-C40) (67.2%) within 140 days under high temperature. Colonization of HT in the soil exhibited lagged characteristics, with a substantial increase in bacterial genera originated from the HT after 60 days. Additionally, LEfSe analysis indicated that the biomarkers of HT treatment were mainly from the HT consortium. Moreover, functional analysis revealed genes related to n-alkane degradation (<em>AlkB, P450</em>, <em>LadA</em>), alkane utilization regulator (<em>AraC, TetR, GntR</em>), as well as several thermotolerance genes were significantly increased in HT treatment. Additionally, network analysis demonstrated distinct co-occurrence patterns induced by nutrient addition and exogenous consortium, with the latter strengthening interactions and stability of bacterial networks under high temperature. This study represents pioneering investigation into the effects of exogenous thermophilic consortium on petroleum degradation, bacterial communities, functional genes and ecological interactions in application of petroleum remediation under thermophilic conditions.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"123 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142974882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cadmium (Cd) is recognized as one of the most toxic heavy metal in the environment that causes pronounced phytotoxicity. This study investigated the physiological and biochemical responses and detoxification mechanisms of Hemarthria compressa under various concentrations of Cd stress (0, 30, 60, 90, and 270 mg·kg-1). Our research findings indicate that the growth and photosynthetic capacity of H. compressa reach their peak at a Cd concentration of 60 mg·kg-1. At this concentration, the Cd concentration in the shoots of H. compressa is 0.67 mg·kg-1, the total Cd accumulation is 0.25 μg, and the MDA content is 6.25 nmol·g-1, which represents the lowest values among all treatments.Metabolomics analysis reveals that sugar is related to Cd stress resistance, and the levels of organic acids involved in metabolic processes show only minor changes. H. compressa alters the composition of its root exudates by secreting substantial quantities of organic acids (such as citric acid, fumaric acid, and malic acid), sugars (such as trehalose, maltose, and glucose), and fatty acids (such as citraconic acid). These organic acids modulate the pH of the rhizosphere soil and recruit beneficial microorganisms, including Gp6, Sphingoaurantiacus, Devosia, and Neobacillus species, thereby enhancing plant growth and mitigating Cd accumulation.
{"title":"Metabolomics and microbiome analysis elucidate the detoxification mechanisms of Hemarthria compressa, a low cadmium accumulating plant, in response to cadmium stress","authors":"Hao Jing, Xiaoliang Xue, Xin Zhang, Xianji Xu, Yuzhou Tang, Hongji Wang, Jiaqi Zheng, Hongyuan Yang, Yuzhu Han","doi":"10.1016/j.jhazmat.2025.137226","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.137226","url":null,"abstract":"Cadmium (Cd) is recognized as one of the most toxic heavy metal in the environment that causes pronounced phytotoxicity. This study investigated the physiological and biochemical responses and detoxification mechanisms of <em>Hemarthria compressa</em> under various concentrations of Cd stress (0, 30, 60, 90, and 270<!-- --> <!-- -->mg·kg<sup>-1</sup>). Our research findings indicate that the growth and photosynthetic capacity of <em>H. compressa</em> reach their peak at a Cd concentration of 60<!-- --> <!-- -->mg·kg<sup>-1</sup>. At this concentration, the Cd concentration in the shoots of <em>H. compressa</em> is 0.67<!-- --> <!-- -->mg·kg<sup>-1</sup>, the total Cd accumulation is 0.25<!-- --> <!-- -->μg, and the MDA content is 6.25 nmol·g<sup>-1</sup>, which represents the lowest values among all treatments.Metabolomics analysis reveals that sugar is related to Cd stress resistance, and the levels of organic acids involved in metabolic processes show only minor changes. <em>H. compressa</em> alters the composition of its root exudates by secreting substantial quantities of organic acids (such as citric acid, fumaric acid, and malic acid), sugars (such as trehalose, maltose, and glucose), and fatty acids (such as citraconic acid). These organic acids modulate the pH of the rhizosphere soil and recruit beneficial microorganisms, including <em>Gp6</em>, <em>Sphingoaurantiacus</em>, <em>Devosia</em>, and <em>Neobacillus</em> species, thereby enhancing plant growth and mitigating Cd accumulation.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"20 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142981640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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