Journal of Applied Research on Medicinal and Aromatic Plants最新文献_第8页

Optimized flow cytometry protocol for assessing DNA content in Vanilla (Orchidaceae) species: Insights into crop wild relatives and a commercial hybrid 评估香草（兰科）物种DNA含量的优化流式细胞术方案：对作物野生近缘种和商业杂交品种的见解

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-02-01 DOI: 10.1016/j.jarmap.2024.100612

Jorge Warner , Yesly Camacho-Solís , Víctor M. Jiménez , Alfonso García-Piñeres

Vanilla (Orchidaceae) is a highly valued spice used in a wide range of products. Although wild crop relatives of Vanilla planifolia, the primary cultivated species, may possess traits for crop improvement, they have received limited attention. This study presents an optimized and reproducible flow cytometry protocol adapted to Vanilla leaves, that effectively prevents nuclei clustering and raphide interference. Assessing nuclear C-DNA content and estimating DNA ploidy in succulent plant tissues pose significant challenges. The abundance of polysaccharides and raphides in Vanilla tissues often leads to inaccuracies in cytometric measurements and difficulties in instrument maintenance. To address this issue, we used a nuclei isolation buffer containing 2.0 % (v/v) detergent and utilized the clear upper phase of the filtrate after raphide sedimentation to obtain accurate nuclear DNA content values. We applied this protocol to estimate the C-DNA content of 12 Vanilla species native to Costa Rica and a widely cultivated commercial hybrid. The values ranged from 5.211 ± 0.008 pg in V. planifolia to 9.0 ± 1.3 pg in V. pompona, with no clear distinction between species of the subgenera Vanilla and Xanata. For the first time, we report the 2C-DNA content of V. costaricensis, V. dressleri, V. hartii, V. helleri, V. inodora, V. karen-christianae and the 'Vaitsy' hybrid. Our results confirm the occurrence of partial endoreplication in all samples analyzed. This study contributes to the understanding of less studied wild crop relatives of V. planifolia and provides an improved method for flow cytometric analysis that can be applied to tissues containing mucilaginous compounds and raphides of other plant species.

香草（兰科）是一种非常有价值的香料，广泛用于各种产品。作为主要栽培品种，香草的野生近缘作物虽然具有作物改良的性状，但受到的关注有限。本研究提出了一种优化的、可重复的适用于香草叶片的流式细胞术方案，该方案有效地防止了细胞核聚集和raphide干扰。在多肉植物组织中评估核C-DNA含量和估计DNA倍性是一个重大的挑战。香草组织中丰富的多糖和raphides常常导致细胞测定的不准确性和仪器维护的困难。为了解决这个问题，我们使用了含有2.0 % （v/v）洗涤剂的核分离缓冲液，并在快速沉淀后利用滤液的透明上相获得准确的核DNA含量值。我们应用该方案估计了12种哥斯达黎加本土香草和一种广泛种植的商业杂交香草的C-DNA含量。平叶橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐橐。首次报道了V. costaricensis、V. dressleri、V. hartii、V. helleri、V. inodora、V. karen-christianae及其杂交品种Vaitsy的2C-DNA含量。我们的结果证实了在所有分析的样本中都发生了部分内复制。该研究有助于了解尚不为人所知的平叶卷叶的野生近缘作物，并为流式细胞术分析提供了一种改进的方法，可用于其他植物中含有黏液化合物和rapides的组织。

{"title":"Optimized flow cytometry protocol for assessing DNA content in Vanilla (Orchidaceae) species: Insights into crop wild relatives and a commercial hybrid","authors":"Jorge Warner , Yesly Camacho-Solís , Víctor M. Jiménez , Alfonso García-Piñeres","doi":"10.1016/j.jarmap.2024.100612","DOIUrl":"10.1016/j.jarmap.2024.100612","url":null,"abstract":"<div><div>Vanilla (Orchidaceae) is a highly valued spice used in a wide range of products. Although wild crop relatives of <em>Vanilla planifolia</em>, the primary cultivated species, may possess traits for crop improvement, they have received limited attention. This study presents an optimized and reproducible flow cytometry protocol adapted to <em>Vanilla</em> leaves, that effectively prevents nuclei clustering and raphide interference. Assessing nuclear C-DNA content and estimating DNA ploidy in succulent plant tissues pose significant challenges. The abundance of polysaccharides and raphides in <em>Vanilla</em> tissues often leads to inaccuracies in cytometric measurements and difficulties in instrument maintenance. To address this issue, we used a nuclei isolation buffer containing 2.0 % (v/v) detergent and utilized the clear upper phase of the filtrate after raphide sedimentation to obtain accurate nuclear DNA content values. We applied this protocol to estimate the C-DNA content of 12 <em>Vanilla</em> species native to Costa Rica and a widely cultivated commercial hybrid. The values ranged from 5.211 ± 0.008 pg in <em>V. planifolia</em> to 9.0 ± 1.3 pg in <em>V. pompona</em>, with no clear distinction between species of the subgenera <em>Vanilla</em> and <em>Xanata</em>. For the first time, we report the 2C-DNA content of <em>V. costaricensis</em>, <em>V. dressleri</em>, <em>V. hartii</em>, <em>V. helleri</em>, <em>V. inodora</em>, <em>V. karen-christianae</em> and the 'Vaitsy' hybrid. Our results confirm the occurrence of partial endoreplication in all samples analyzed. This study contributes to the understanding of less studied wild crop relatives of <em>V. planifolia</em> and provides an improved method for flow cytometric analysis that can be applied to tissues containing mucilaginous compounds and raphides of other plant species.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"44 ","pages":"Article 100612"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of genetic diversity and population structure of Pinellia ternata using EST-SSR markers 利用EST-SSR标记分析半夏的遗传多样性和群体结构

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-02-01 DOI: 10.1016/j.jarmap.2024.100611

Mingxing Wang , Rong Xu , Ming Luo , Xinyao Li , Lu Wei , Mi Lei , Dahui Liu , Yuhuan Miao

Pinellia ternata (Thunb.) Breit. is a widely used Chinese Medicinal Material with economic and medicinal value. Few molecular studies have been conducted on P. ternata, resulting in a lack of applicable molecular markers. This present research analyzed the genetic diversity of 255 P. ternata germplasm resources from China, including 117 wild germplasm resources and 138 cultivated germplasm resources. Following the development of 17 pairs of highly polymorphic primers, 126 alleles were found; the average polymorphism was 99.21 %. The average number of observed alleles (Na), effective alleles (Ne) were 1.98 and 1.36, respectively. The mean of the Nei genetic diversity index (H), the Shannon's information index (I) and the polymorphic information content (PIC) were 0.23, 0.36 and 0.6482, respectively. The population structure of P. ternata germplasm resources was categorized into two groups, designated as Group 1 and Group 2. The majority of wild germplasm resources was clustered in Group 1. Phylogenetic and population structure analyses produced similar results. A comparison of the genetic diversity parameters of wild and cultivated germplasm revealed that the genetic diversity was higher in the former, while the genetic differentiation level of the two types of germplasm was low. Analysis of molecular variance (AMOVA) demonstrated that 9 % of the genetic variation was between populations and 91 % of the genetic variation was within populations. Principal coordinate analysis (PCoA) served to corroborate the findings of the cluster and population structure analysis. It was found that P. ternata with tubers having red epidermis was more separated from other germplasm resources. Association analysis based on the generalized linear model (GLM) detected four EST-SSR markers significantly associated with P. ternata tuber color. This study will be helpful for P. ternata germplasm resource conservation, identification and trait-specific breeding programs.

半夏（Pinellia ternata）布莱特。是一种广泛使用的中药材，具有经济和药用价值。目前国内外对紫檀的分子研究较少，缺乏适用的分子标记。本研究分析了255 P的遗传多样性。其中，野生种质资源117种，栽培种质资源138种。经过17对高多态性引物的开发，共发现126个等位基因；平均多态性为99.21 %。平均观察到的等位基因数（Na）和有效等位基因数（Ne）分别为1.98和1.36。Nei遗传多样性指数(H)、Shannon信息指数(I)和多态性信息含量（PIC）的平均值分别为0.23、0.36和0.6482。柽柳种质资源的种群结构可划分为2个类群，分别为类群1和类群2。大部分野生种质资源集中在类群1。系统发育和种群结构分析也得出了类似的结果。野生和栽培种质的遗传多样性参数比较表明，野生和栽培种质的遗传多样性较高，而两种种质的遗传分化水平较低。分子变异分析（AMOVA）表明，9 %的遗传变异发生在群体间，91 %的遗传变异发生在群体内。主坐标分析（PCoA）证实了聚类分析和种群结构分析的结果。结果表明，块茎表皮呈红色的藤属植物与其他种质资源的分离程度较高。基于广义线性模型（GLM）的关联分析检测到4个EST-SSR标记与块茎颜色显著相关。本研究将为柽柳种质资源的保护、鉴定和性状选育提供依据。

{"title":"Analysis of genetic diversity and population structure of Pinellia ternata using EST-SSR markers","authors":"Mingxing Wang , Rong Xu , Ming Luo , Xinyao Li , Lu Wei , Mi Lei , Dahui Liu , Yuhuan Miao","doi":"10.1016/j.jarmap.2024.100611","DOIUrl":"10.1016/j.jarmap.2024.100611","url":null,"abstract":"<div><div><em>Pinellia ternata</em> (Thunb.) Breit. is a widely used Chinese Medicinal Material with economic and medicinal value. Few molecular studies have been conducted on <em>P. ternata</em>, resulting in a lack of applicable molecular markers. This present research analyzed the genetic diversity of 255 <em>P. ternata</em> germplasm resources from China, including 117 wild germplasm resources and 138 cultivated germplasm resources. Following the development of 17 pairs of highly polymorphic primers, 126 alleles were found; the average polymorphism was 99.21 %. The average number of observed alleles (<em>Na</em>), effective alleles (<em>Ne</em>) were 1.98 and 1.36, respectively. The mean of the Nei genetic diversity index (<em>H</em>), the Shannon's information index (<em>I</em>) and the polymorphic information content (<em>PIC</em>) were 0.23, 0.36 and 0.6482, respectively. The population structure of <em>P. ternata</em> germplasm resources was categorized into two groups, designated as Group 1 and Group 2. The majority of wild germplasm resources was clustered in Group 1. Phylogenetic and population structure analyses produced similar results. A comparison of the genetic diversity parameters of wild and cultivated germplasm revealed that the genetic diversity was higher in the former, while the genetic differentiation level of the two types of germplasm was low. Analysis of molecular variance (AMOVA) demonstrated that 9 % of the genetic variation was between populations and 91 % of the genetic variation was within populations. Principal coordinate analysis (PCoA) served to corroborate the findings of the cluster and population structure analysis. It was found that <em>P. ternata</em> with tubers having red epidermis was more separated from other germplasm resources. Association analysis based on the generalized linear model (GLM) detected four EST-SSR markers significantly associated with <em>P. ternata</em> tuber color. This study will be helpful for <em>P. ternata</em> germplasm resource conservation, identification and trait-specific breeding programs.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"44 ","pages":"Article 100611"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of the extraction of bioactive compounds from the leaves of Campomanesia sessiliflora (O. Berg) Mattos: Standardizing the utilization of a new resource 山芋叶中生物活性物质的提取工艺优化：新资源的规范化利用

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-02-01 DOI: 10.1016/j.jarmap.2024.100615

Raquel Parra Loureiro , Thiago Luis Aguayo de Castro , Claudia Andrea Lima Cardoso

The use of medicinal plants that produce a secondary metabolite continues to grow along with population growth. One of these species is Campomanesia sessiliflora (O. Berg) Mattos. The literature reports that C. sessiliflora is rich in phenolic compounds and flavonoids, which possess high anti-aging activity and can be utilized in the cosmetic and food industries. Optimizing the extraction of bioactive compounds from this plant is crucial for its industrial application. Currently, Campomanesia sessiliflora leaves are discarded as pruning waste. The evaluation of secondary metabolites from C. sessiliflora leaves is essential to making their use feasible for obtaining antioxidant and photoprotective extracts. This study aims to optimize the extraction of phenolic compounds, flavonoids, tannins, chlorophyll α, chlorophyll β, and carotenoids, as well as evaluate the antioxidant potential, sun protection factor (SPF), and the ultraviolet region scans of the leaf extracts. Liquid chromatography with a diode array detector was used to identify chemical compounds. The extracts were evaluated using different plant-to-solvent ratios and varying ethanol concentrations in water. A correlation test was conducted between the spectrophotometric profile and the levels of phenolic compounds, flavonoids, tannins, and the antioxidant potential of C. sessiliflora leaf extracts. Results showed a positive correlation between ethanol levels and the presence of chlorophyll α, chlorophyll β, carotenoids, and flavonoids. Additionally, a correlation was found between antioxidant potential, SPF, and phenolic compound content. Statistical analyses indicate that the ethanol concentration in water is more significantly relevant than the different plant-to-solvent proportions. Gallic acid, syringic acid, catechin, epicatechin, and rutin were identified in all samples. Absorption at the 260 nm wavelength can be used for extract quality control. Regarding the monitored parameters, the best extract was obtained using 2 % leaves in 25 % ethanol in water.

随着人口的增长，对产生次生代谢物的药用植物的使用也在继续增长。其中一种是Campomanesia sessiliflora (O. Berg) Mattos。文献报道无梗草含有丰富的酚类化合物和黄酮类化合物，具有较高的抗衰老活性，可用于化妆品和食品工业。优化从该植物中提取生物活性化合物的工艺对其工业应用至关重要。目前，无梗木芋叶作为修剪废弃物被丢弃。无梗草叶次生代谢物的评价是确定其抗氧化和光保护提取物的重要依据。本研究旨在优化黄酮类化合物、单宁、叶绿素α、叶绿素β和类胡萝卜素的提取工艺，并对叶提取物的抗氧化能力、防晒系数（SPF）和紫外区扫描进行评价。采用二极管阵列检测器的液相色谱法对化合物进行了鉴定。采用不同的植物与溶剂比和不同的乙醇浓度对提取物进行了评价。采用分光光度法对无梗草叶提取物中酚类化合物、黄酮类化合物、单宁含量及抗氧化活性进行了相关性分析。结果表明，乙醇水平与叶绿素α、叶绿素β、类胡萝卜素和黄酮类化合物的含量呈正相关。此外，还发现抗氧化能力、SPF和酚类化合物含量之间存在相关性。统计分析表明，与不同的植物-溶剂比例相比，水中乙醇浓度的相关性更显著。在所有样品中均鉴定出没食子酸、丁香酸、儿茶素、表儿茶素和芦丁。260 nm吸收波长可用于提取液的质量控制。根据监测参数，以2 %的叶片和25 %的乙醇水溶液为最佳提取液。

{"title":"Optimization of the extraction of bioactive compounds from the leaves of Campomanesia sessiliflora (O. Berg) Mattos: Standardizing the utilization of a new resource","authors":"Raquel Parra Loureiro , Thiago Luis Aguayo de Castro , Claudia Andrea Lima Cardoso","doi":"10.1016/j.jarmap.2024.100615","DOIUrl":"10.1016/j.jarmap.2024.100615","url":null,"abstract":"<div><div>The use of medicinal plants that produce a secondary metabolite continues to grow along with population growth. One of these species is <em>Campomanesia sessiliflora</em> (O. Berg) Mattos. The literature reports that <em>C. sessiliflora</em> is rich in phenolic compounds and flavonoids, which possess high anti-aging activity and can be utilized in the cosmetic and food industries. Optimizing the extraction of bioactive compounds from this plant is crucial for its industrial application. Currently, <em>Campomanesia sessiliflora</em> leaves are discarded as pruning waste. The evaluation of secondary metabolites from C. sessiliflora leaves is essential to making their use feasible for obtaining antioxidant and photoprotective extracts. This study aims to optimize the extraction of phenolic compounds, flavonoids, tannins, chlorophyll α, chlorophyll β, and carotenoids, as well as evaluate the antioxidant potential, sun protection factor (SPF), and the ultraviolet region scans of the leaf extracts. Liquid chromatography with a diode array detector was used to identify chemical compounds. The extracts were evaluated using different plant-to-solvent ratios and varying ethanol concentrations in water. A correlation test was conducted between the spectrophotometric profile and the levels of phenolic compounds, flavonoids, tannins, and the antioxidant potential of <em>C. sessiliflora</em> leaf extracts. Results showed a positive correlation between ethanol levels and the presence of chlorophyll α, chlorophyll β, carotenoids, and flavonoids. Additionally, a correlation was found between antioxidant potential, SPF, and phenolic compound content. Statistical analyses indicate that the ethanol concentration in water is more significantly relevant than the different plant-to-solvent proportions. Gallic acid, syringic acid, catechin, epicatechin, and rutin were identified in all samples. Absorption at the 260 nm wavelength can be used for extract quality control. Regarding the monitored parameters, the best extract was obtained using 2 % leaves in 25 % ethanol in water.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"44 ","pages":"Article 100615"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and physiological plasticity of alpine medicinal plants along an elevational gradient 高山药用植物在海拔梯度上的形态和生理可塑性

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-02-01 DOI: 10.1016/j.jarmap.2024.100613

Xuemei Wu , Liwen Zhong , Guopeng Chen , Shihong Zhong , Rui Gu

Alpine medicinal plant growth, physiological processes and final number of metabolites are affected by elevation, demonstrating phenotypic plasticity. Therefore, in-depth understanding the alpine medicinal plant behaviors in responding to shift elevation has actually implications for resources protection, sustainable utilization and domestication cultivation. Currently, lots of literature detailly studied the effect of environmental factors on the synthesis and metabolism of second metabolisms in medicinal plants while the responding results of phenotype and metabolism to constrain environmental still lack systematic classification and summary. Hence, basing on previous works and literature, the adjustment of morphology and metabolism of alpine medicinal plants in responding of shift elevation were systematically outlined. The results of this reviews can know how the alpine plant adapting to shift elevation through physiological, metabolic and morphological adaptation. And this knowledge can be integrated into a framework for domesticating and protecting alpine medicinal plants, and can also serve as a quick tool to study the effects of climate warming on plateau medicinal plants.

高山药用植物的生长、生理过程和最终代谢物数量受到海拔的影响，表现出表型可塑性。因此，深入了解高寒药用植物对海拔变化的响应行为，对资源保护、可持续利用和驯化栽培具有重要意义。目前，大量文献详细研究了环境因素对药用植物第二代谢产物合成和代谢的影响，而表型和代谢对环境约束的响应结果仍缺乏系统的分类和总结。因此，在前人研究和文献的基础上，系统概述了高寒药用植物对海拔变化的形态和代谢调节。本文综述了高寒植物是如何通过生理、代谢和形态适应来适应海拔变化的。这些知识可以整合到高山药用植物驯化和保护的框架中，也可以作为研究气候变暖对高原药用植物影响的快速工具。

{"title":"Morphological and physiological plasticity of alpine medicinal plants along an elevational gradient","authors":"Xuemei Wu , Liwen Zhong , Guopeng Chen , Shihong Zhong , Rui Gu","doi":"10.1016/j.jarmap.2024.100613","DOIUrl":"10.1016/j.jarmap.2024.100613","url":null,"abstract":"<div><div>Alpine medicinal plant growth, physiological processes and final number of metabolites are affected by elevation, demonstrating phenotypic plasticity. Therefore, in-depth understanding the alpine medicinal plant behaviors in responding to shift elevation has actually implications for resources protection, sustainable utilization and domestication cultivation. Currently, lots of literature detailly studied the effect of environmental factors on the synthesis and metabolism of second metabolisms in medicinal plants while the responding results of phenotype and metabolism to constrain environmental still lack systematic classification and summary. Hence, basing on previous works and literature, the adjustment of morphology and metabolism of alpine medicinal plants in responding of shift elevation were systematically outlined. The results of this reviews can know how the alpine plant adapting to shift elevation through physiological, metabolic and morphological adaptation. And this knowledge can be integrated into a framework for domesticating and protecting alpine medicinal plants, and can also serve as a quick tool to study the effects of climate warming on plateau medicinal plants.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"44 ","pages":"Article 100613"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of gibberellic acid treatment and cold stratification on breaking combined (physiological + mechanical) dormancy and germination in Sassafras tzumu (Hemsl.) Hemsl seeds 赤霉素酸处理和冷分层对黄樟破（生理+机械）联合休眠和萌发的影响Hemsl种子

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-02-01 DOI: 10.1016/j.jarmap.2024.100606

Yu Wu , Zhi Yun Deng , Ming Zhu Wang , Lin Yue Liu , Yong Bao Shen

Sassafras tzumu (Hemsl.) Hemsl., known as Chinese sassafras, is an exclusive species of tree found in China, belonging to the Sassafras genus in the Lauraceae family. Its primary mode of propagation is through seeds, which possesses a profound dormancy and irregular germination. In its natural environment, it takes approximately 2–3 years for the seeds to emerge from the ground and begin germinating. Consequently, a rapid and effective method for breaking dormancy would greatly benefitsbn edjhn the production of S. tzumu seedlings. The objective of this investigation was to identify the optimal treatment for breaking dormancy. The S. tzumu seeds were subjected to gibberellic acid (GA₃) treatment and cold stratification. The findings indicated that the dormancy type of Sassafras tzumu seeds was combined (physiological + mechanical) dormancy. After 6 months of cold stratification, the germination percentage of seeds treated with various concentrations of GA₃ (200 mg/L, 500 mg/L, and 1000 mg/L) all exceeded 50 %. Among these treatments, the most effective method was the application of 200 mg/L GA₃ in combination with 5 months of cold stratification, resulting in a germination percentage of 55 %. During the process of cold stratification, soluble proteins, soluble sugars, starch, and fat were broken down into smaller molecules to support embryo growth. Interestingly, the content of soluble sugar exhibited an upward-downward trend, while the content of soluble protein displayed a downward-upward trend. The release of dormancy in S. tzumu seeds was jointly regulated by various endogenous hormones. The content of GA₃ demonstrated an upward-downward trend, while the ABA content exhibited a continuous decline with a brief upward trend. In contrast, the IAA content displayed a continuous upward trend, and the ZR content showed no significant change. The ratios of GA₃/ABA, IAA/ABA, and ZR/ABA all displayed an upward trend.

黄樟（木）Hemsl。中国檫木是中国特有的树种，属于樟科檫木属。其主要繁殖方式为种子，休眠深，萌发不规律。在自然环境中，种子大约需要2-3年才能从地下萌发并开始发芽。因此，寻找一种快速有效的方法来打破休眠，将极大地促进紫豆幼苗的生产。本研究的目的是确定打破休眠的最佳处理方法。采用赤霉素（GA3）处理和冷分层。结果表明，黄樟种子休眠类型为生理+机械组合休眠。冷分层6个月后，不同浓度GA3（200 mg/L、500 mg/L和1000 mg/L）处理的种子发芽率均超过50% %。其中，200 mg/L GA3加5个月的冷分层处理最有效，发芽率为55 %。在冷分层过程中，可溶性蛋白、可溶性糖、淀粉和脂肪被分解成支持胚胎生长的小分子。有趣的是，可溶性糖含量呈上升-下降趋势，可溶性蛋白含量呈下降-上升趋势。枸杞种子的休眠释放受多种内源激素的共同调控。GA3含量呈上升-下降趋势，ABA含量呈持续下降趋势，并有短暂上升趋势。IAA含量呈持续上升趋势，ZR含量变化不显著。GA3/ABA、IAA/ABA、ZR/ABA均呈上升趋势。

{"title":"Effect of gibberellic acid treatment and cold stratification on breaking combined (physiological + mechanical) dormancy and germination in Sassafras tzumu (Hemsl.) Hemsl seeds","authors":"Yu Wu , Zhi Yun Deng , Ming Zhu Wang , Lin Yue Liu , Yong Bao Shen","doi":"10.1016/j.jarmap.2024.100606","DOIUrl":"10.1016/j.jarmap.2024.100606","url":null,"abstract":"<div><div><em>Sassafras tzumu</em> (Hemsl.) Hemsl., known as Chinese sassafras, is an exclusive species of tree found in China, belonging to the <em>Sassafras</em> genus in the Lauraceae family. Its primary mode of propagation is through seeds, which possesses a profound dormancy and irregular germination. In its natural environment, it takes approximately 2–3 years for the seeds to emerge from the ground and begin germinating. Consequently, a rapid and effective method for breaking dormancy would greatly benefitsbn edjhn the production of <em>S. tzumu</em> seedlings. The objective of this investigation was to identify the optimal treatment for breaking dormancy. The <em>S. tzumu</em> seeds were subjected to gibberellic acid (GA<sub>3</sub>) treatment and cold stratification. The findings indicated that the dormancy type of <em>Sassafras tzumu</em> seeds was combined (physiological + mechanical) dormancy. After 6 months of cold stratification, the germination percentage of seeds treated with various concentrations of GA<sub>3</sub> (200 mg/L, 500 mg/L, and 1000 mg/L) all exceeded 50 %. Among these treatments, the most effective method was the application of 200 mg/L GA<sub>3</sub> in combination with 5 months of cold stratification, resulting in a germination percentage of 55 %. During the process of cold stratification, soluble proteins, soluble sugars, starch, and fat were broken down into smaller molecules to support embryo growth. Interestingly, the content of soluble sugar exhibited an upward-downward trend, while the content of soluble protein displayed a downward-upward trend. The release of dormancy in <em>S. tzumu</em> seeds was jointly regulated by various endogenous hormones. The content of GA<sub>3</sub> demonstrated an upward-downward trend, while the ABA content exhibited a continuous decline with a brief upward trend. In contrast, the IAA content displayed a continuous upward trend, and the ZR content showed no significant change. The ratios of GA<sub>3</sub>/ABA, IAA/ABA, and ZR/ABA all displayed an upward trend.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"44 ","pages":"Article 100606"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using microwave-assisted extraction with advanced artificial intelligence models for predicting tannins in black pepper (Piper nigrum L.) 微波辅助提取与先进人工智能模型预测黑胡椒（Piper nigrum L.）单宁含量

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-02-01 DOI: 10.1016/j.jarmap.2024.100594

Mostafa Khajeh , Mansour Ghaffari-Moghaddam , Jamshid Piri , Afsaneh Barkhordar , Halil Şenol , Didem Saloglu

Black pepper (Piper nigrum L.) is a widely used spice that provides great potential for research in the field of natural products. In this work, the recovery of tannins from black pepper was conducted using microwave-assisted extraction (MAE). The study involves four independent variables: power (from 100 to 300 W), extraction time (from 10 to 40 minutes), temperature (from 35 to 50 °C), and the ratio of food to solvent (from 0.25 to 0.5 g/10 mL). The response variable was the extraction yield, which is the total tannin content. A total of 30 different experimental runs were completed in the MAE system. An evaluation and comparison of two non-verbal modeling approaches and artificial intelligence-based models was conducted. In order to predict design performance and results, the three SVR-RSM, M5Tree, and RM5Tree models were compared to a proposed nonlinear regression model. Evaluations were conducted using health criteria such as RMSE and NSE. With an RMSE of 0.035 and an NSE of 0.91, the SVR-RSM algorithm showed the highest level of accuracy. A RMSE of 0.048 and an NSE of 0.83 is obtained from the RM5tree model, while a RMSE of 0.055 and an NSE of 0.78 is obtained from the M5Tree model. Also, an NSE of 0.65 and a RMSE of 0.068 were obtained for the proposed nonlinear model. The SVR-RSM algorithm had maximum accuracy, but tree models for systems requiring a quick response are the right options. Using the proposed non-error model, complex relationships between variables could also be modeled.

黑胡椒（Piper nigrum L.）是一种应用广泛的香料，在天然产物领域具有很大的研究潜力。采用微波辅助萃取法从黑胡椒中提取单宁。该研究涉及四个自变量：功率（从100到300 W），提取时间（从10到40 分钟），温度（从35到50°C），以及食物与溶剂的比例（从0.25到0.5 g/10 mL）。响应变量为提取率，即总单宁含量。在MAE系统中共完成了30次不同的实验运行。对两种非语言建模方法和基于人工智能的模型进行了评价和比较。为了预测设计性能和结果，将SVR-RSM、M5Tree和RM5Tree三种模型与提出的非线性回归模型进行了比较。使用RMSE和NSE等健康标准进行评估。SVR-RSM算法的RMSE为0.035，NSE为0.91，显示出最高的精度。RM5tree模型的RMSE为0.048，NSE为0.83，而M5Tree模型的RMSE为0.055，NSE为0.78。该模型的NSE为0.65，RMSE为0.068。SVR-RSM算法具有最高的精度，但对于需要快速响应的系统，树模型是正确的选择。利用提出的非误差模型，变量之间的复杂关系也可以建模。

{"title":"Using microwave-assisted extraction with advanced artificial intelligence models for predicting tannins in black pepper (Piper nigrum L.)","authors":"Mostafa Khajeh , Mansour Ghaffari-Moghaddam , Jamshid Piri , Afsaneh Barkhordar , Halil Şenol , Didem Saloglu","doi":"10.1016/j.jarmap.2024.100594","DOIUrl":"10.1016/j.jarmap.2024.100594","url":null,"abstract":"<div><div>Black pepper (<em>Piper nigrum</em> L.) is a widely used spice that provides great potential for research in the field of natural products. In this work, the recovery of tannins from black pepper was conducted using microwave-assisted extraction (MAE). The study involves four independent variables: power (from 100 to 300 W), extraction time (from 10 to 40 minutes), temperature (from 35 to 50 °C), and the ratio of food to solvent (from 0.25 to 0.5 g/10 mL). The response variable was the extraction yield, which is the total tannin content. A total of 30 different experimental runs were completed in the MAE system. An evaluation and comparison of two non-verbal modeling approaches and artificial intelligence-based models was conducted. In order to predict design performance and results, the three SVR-RSM, M5Tree, and RM5Tree models were compared to a proposed nonlinear regression model. Evaluations were conducted using health criteria such as RMSE and NSE. With an RMSE of 0.035 and an NSE of 0.91, the SVR-RSM algorithm showed the highest level of accuracy. A RMSE of 0.048 and an NSE of 0.83 is obtained from the RM5tree model, while a RMSE of 0.055 and an NSE of 0.78 is obtained from the M5Tree model. Also, an NSE of 0.65 and a RMSE of 0.068 were obtained for the proposed nonlinear model. The SVR-RSM algorithm had maximum accuracy, but tree models for systems requiring a quick response are the right options. Using the proposed non-error model, complex relationships between variables could also be modeled.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"44 ","pages":"Article 100594"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology and phylogeny of Calonectria elaeocarpi sp. nov., a new foliar pathogen of Elaeocarpus sphaericus (Gaertn.) Heer from India 球果叶面新病原菌elaeocarpi sp. nov.的形态与系统发育来自印度的海尔

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-02-01 DOI: 10.1016/j.jarmap.2024.100614

Nitika Bansal , Anju Rani , Shailesh Pandey

Elaeocarpus sphaericus is one of the most important medicinal species in the ancient Indian system of medicine. In 2023, leaf spots were observed on E. sphaericus trees in Dehradun, India. Morphological features and phylogenetic analyses of combined partial actin, calmodulin, histone, internal transcribed spacer, translation elongation factor 1-alpha and β-tubulin regions confirmed the association of a new fungal species of Nectriaceae, which is illustrated and described as Calonectria elaeocarpi sp. nov. In pathogenicity tests, this species caused disease symptoms on the healthy leaves of E. sphaericus and successfully re-isolated, thus fulfilling Koch’s postulates. This study is the first confirmed report of a new Calonectria species causing foliar symptoms on E. sphaericus in India. Future studies are warranted to monitor its natural host range and understand cross infectivity potential.

球菖蒲（Elaeocarpus sphaericus）是古印度医学体系中最重要的药用植物之一。2023年，在印度德拉敦（Dehradun）地区，发现了球形叶斑病。结合部分肌动蛋白、钙调蛋白、组蛋白、内部转录间隔区、翻译延伸因子1- α和β-微管蛋白区域的形态特征和系统发育分析，证实了一种新的真菌物种的关联，说明并描述为Calonectria elaeocarpi sp. 11 .在致病性试验中，该物种在E. sphaericus的健康叶片上引起疾病症状并成功重新分离，从而实现了Koch的假设。本研究是在印度首次证实的一种新的Calonectria种引起球形叶面症状的报告。未来的研究需要监测其自然宿主范围和了解交叉感染的潜力。

引用次数: 0

Extraction of polyphenolic compounds from rose and marigold, UPLC-ESI-QToF-MS/MS, FTIR characterization and assessment of antioxidant activity 玫瑰和万寿菊中多酚类化合物的提取、UPLC-ESI-QToF-MS/MS、FTIR表征及抗氧化活性评价

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-02-01 DOI: 10.1016/j.jarmap.2024.100608

Lekshmi SG , Shruti Sethi , Aditi Kundu , Anupama Singh , Kanwar Pal Singh , Namita , Rajesh Kumar , Ram Asrey , P.M. Sindhu

This study aimed to optimize the process for effective extraction of polyphenolic compounds, flavanoids and antioxidant activity of rose and marigold leaf (RL, ML) and petal (RP, MP) extracts and to analyse their composition. Various drying methods, such as shade, oven (40–70 °C), and microwave (600 W) were utilized for extraction of bioactives with three solvents (water, ethanol, methanol). Microwave dried methanolic extracted samples exhibited high quantities of total phenols, flavanoids, and antioxidants for RL, ML, and MP, while aqueous extracts were found to be the best for extracting bioactives from RP. As the drying temperature increased, the colour values of the extracts decreased. Pigments (chlorophyll, anthocyanin, carotenoids) degraded when the drying temperature increased. The ultra-performance liquid chromatography (UPLC) and fourier transform infrared (FTIR) analysis revealed the primary polyphenol compounds in rose as quercetin, quercetin 3-O-rutinoside, and kaempferol acetyl disaccharide while in marigold they were identified as kaempferol, epicatechin gallate, and feruloyl-caffeoylquinic acid). Also 126 other polyphenolic compounds were identified putatively from rose and marigold through UPLC-ESI-QToF-MS/MS and FTIR. Marigold and rose foliage accounts for 58 % and 82 % of the entire plant's fresh weight, respectively that is usually disposed off as waste. The floral fraction is typically discarded after it has served its primary function. The process of extracting bioactive compounds from these medicinal crops can enhance their value and yield useful products having varying end-use.

本研究旨在优化玫瑰和万寿菊叶片（RL， ML）和花瓣（RP， MP）提取物中多酚类化合物、黄酮类化合物的有效提取工艺，并对其成分进行分析。采用遮阳、烘箱（40-70 °C）和微波（600 W）等多种干燥方法，以水、乙醇、甲醇三种溶剂提取生物活性物质。微波干燥的甲醇提取样品显示出大量的总酚、黄酮类化合物和抗氧化剂，用于RL、ML和MP，而水提取物被发现是提取RP中生物活性的最佳方法。随着干燥温度的升高，提取物的颜色值降低。当干燥温度升高时，色素（叶绿素、花青素、类胡萝卜素）降解。超高效液相色谱（UPLC）和傅里叶变换红外（FTIR）分析表明，玫瑰中的主要多酚类化合物为槲皮素、槲皮素3- o -芦丁苷和山奈酚乙酰二糖，万寿菊中的主要多酚类化合物为山奈酚、表儿茶素没食子酸酯和阿魏酰咖啡酰奎宁酸。另外，通过UPLC-ESI-QToF-MS/MS和FTIR等方法从玫瑰和万寿菊中鉴定出126种多酚类化合物。金盏花和玫瑰的叶子分别占整个植物鲜重的58% %和82， %，通常作为废物处理掉。花的部分通常在完成其主要功能后被丢弃。从这些药用作物中提取生物活性化合物的过程可以提高它们的价值，并产生具有不同最终用途的有用产品。

{"title":"Extraction of polyphenolic compounds from rose and marigold, UPLC-ESI-QToF-MS/MS, FTIR characterization and assessment of antioxidant activity","authors":"Lekshmi SG , Shruti Sethi , Aditi Kundu , Anupama Singh , Kanwar Pal Singh , Namita , Rajesh Kumar , Ram Asrey , P.M. Sindhu","doi":"10.1016/j.jarmap.2024.100608","DOIUrl":"10.1016/j.jarmap.2024.100608","url":null,"abstract":"<div><div>This study aimed to optimize the process for effective extraction of polyphenolic compounds, flavanoids and antioxidant activity of rose and marigold leaf (RL, ML) and petal (RP, MP) extracts and to analyse their composition. Various drying methods, such as shade, oven (40–70 °C), and microwave (600 W) were utilized for extraction of bioactives with three solvents (water, ethanol, methanol). Microwave dried methanolic extracted samples exhibited high quantities of total phenols, flavanoids, and antioxidants for RL, ML, and MP, while aqueous extracts were found to be the best for extracting bioactives from RP. As the drying temperature increased, the colour values of the extracts decreased. Pigments (chlorophyll, anthocyanin, carotenoids) degraded when the drying temperature increased. The ultra-performance liquid chromatography (UPLC) and fourier transform infrared (FTIR) analysis revealed the primary polyphenol compounds in rose as quercetin, quercetin 3-O-rutinoside, and kaempferol acetyl disaccharide while in marigold they were identified as kaempferol, epicatechin gallate, and feruloyl-caffeoylquinic acid). Also 126 other polyphenolic compounds were identified putatively from rose and marigold through UPLC-ESI-QToF-MS/MS and FTIR. Marigold and rose foliage accounts for 58 % and 82 % of the entire plant's fresh weight, respectively that is usually disposed off as waste. The floral fraction is typically discarded after it has served its primary function. The process of extracting bioactive compounds from these medicinal crops can enhance their value and yield useful products having varying end-use.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"44 ","pages":"Article 100608"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of pretreatments on seed germination of musk rose (Rosa moschata Herrm.) 预处理对麝香玫瑰种子萌发的影响

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-01-29 DOI: 10.1016/j.jarmap.2025.100628

Vikas Yadav Patade , Nirbhay Singh , Atul Grover , Madhu Bala

We investigated effects of different chemical pre-treatments on seed propagation in musk rose (Rosa moschata Herrm.), an important Himalayan medicinal and aromatic climbing shrub. The seeds were pre-treated with potassium nitrate (150, 300, 450, 600 mM) or polyethylene glycol-PEG 8000 (12.5, 25.0 mM) for 24 or 48 hours. The seed pre-treatments were carried out at 15 °C or 25 °C in a seed germinator. The pre-treatment with potassium nitrate (300, 450 or 600 mM) for 48 h at 15 °C resulted in significantly (P ≤ 0.05) higher germination than the control and other treatments. The seed germination in treatment with 600 mM potassium nitrate (39.71 %) for 48 h at 15 °C was 34 fold higher than in the control (1.16 %). Further, the rate of seed germination (Timson’s Index) was also significantly higher in the potassium nitrate treatments than the control and other treatments. The pre-treatment was found more effective in case of seeds stored for 26 months than the one stored for 13 months. Among the other treatments, pre-treatment with 12.5 mM PEG 8000 for 24 h at 25 °C resulted in significantly higher germination (21.83 %) than the control. The germinating seeds from the treatments successfully established in seedlings and mature plants subsequently. Results of the present study thus suggest the pre-germination seed treatment with 600 mM potassium nitrate solution for higher, synchronous and rapid germination in musk rose for a large scale propagation of this economically and ecologically important Himalayan plant.

研究了不同化学处理对麝香月季种子繁殖的影响。麝香月季是喜马拉雅地区重要的药用和芳香攀缘灌木。用硝酸钾（150、300、450、600 mM）或聚乙二醇- peg 8000（12.5、25.0 mM）预处理种子24或48 小时。种子预处理在15 °C或25 °C的种子萌发器中进行。在15℃条件下，硝酸钾（300、450或600 mM）预处理48 h，发芽率显著高于对照和其他处理（P ≤ 0.05）。600 mM硝酸钾（39.71 %）在15 °C下处理48 h，种子萌发率比对照（1.16 %）高34倍。另外，硝态钾处理的种子发芽率（Timson’s Index）也显著高于对照和其他处理。结果表明，贮藏26个月的种子比贮藏13个月的种子更有效。在其他处理中，12.5 mM PEG 8000在25 °C下预处理24 h，发芽率显著高于对照（21.83 %）。处理后的发芽种子随后成功地在幼苗和成熟植株上生根。因此，本研究结果表明，600 mM硝酸钾溶液在麝香玫瑰种子萌发前处理，可以使这种具有重要经济和生态意义的喜马拉雅植物在大规模繁殖中更高、同步和快速发芽。

{"title":"Effect of pretreatments on seed germination of musk rose (Rosa moschata Herrm.)","authors":"Vikas Yadav Patade , Nirbhay Singh , Atul Grover , Madhu Bala","doi":"10.1016/j.jarmap.2025.100628","DOIUrl":"10.1016/j.jarmap.2025.100628","url":null,"abstract":"<div><div>We investigated effects of different chemical pre-treatments on seed propagation in musk rose (<em>Rosa moschata</em> Herrm.), an important Himalayan medicinal and aromatic climbing shrub. The seeds were pre-treated with potassium nitrate (150, 300, 450, 600 mM) or polyethylene glycol-PEG 8000 (12.5, 25.0 mM) for 24 or 48 hours. The seed pre-treatments were carried out at 15 °C or 25 °C in a seed germinator. The pre-treatment with potassium nitrate (300, 450 or 600 mM) for 48 h at 15 °C resulted in significantly (<em>P</em> ≤ 0.05) higher germination than the control and other treatments. The seed germination in treatment with 600 mM potassium nitrate (39.71 %) for 48 h at 15 °C was 34 fold higher than in the control (1.16 %). Further, the rate of seed germination (Timson’s Index) was also significantly higher in the potassium nitrate treatments than the control and other treatments. The pre-treatment was found more effective in case of seeds stored for 26 months than the one stored for 13 months. Among the other treatments, pre-treatment with 12.5 mM PEG 8000 for 24 h at 25 °C resulted in significantly higher germination (21.83 %) than the control. The germinating seeds from the treatments successfully established in seedlings and mature plants subsequently. Results of the present study thus suggest the pre-germination seed treatment with 600 mM potassium nitrate solution for higher, synchronous and rapid germination in musk rose for a large scale propagation of this economically and ecologically important Himalayan plant.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"45 ","pages":"Article 100628"},"PeriodicalIF":3.8,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143275755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of ultrasound phenolic extraction from Erica multiflora leaves using response surface methodology and artificial neural networks 基于响应面法和人工神经网络的超声多酚提取工艺优化

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-01-22 DOI: 10.1016/j.jarmap.2025.100627

Anfel Benmanseur , Mustapha Tacherfiout , Yacine Benguerba , Fatma Zahra Hab , Rechda Amel Tachour , Bachra Khettal , Rania Derguine , Mostapha Bachir-Bey , Abdelmalek Rezgui , Widad Sobhi

This study aimed to develop and optimize an efficient ultrasound-assisted extraction method for phenolic compounds from Erica multiflora leaves, with the primary objectives of preserving extract quality, minimizing solvent usage, and standardizing extraction conditions. A total of 15 extractions were performed, varying solvent concentration, extraction time, and ultrasonic amplitude, using a Box-Behnken design. The effects of the extraction parameters on phenolic yield were modeled using response surface methodology (RSM) and artificial neural networks (ANN). Both models demonstrated strong predictive capabilities for all measured responses. Among the extraction parameters, amplitude and time were identified as key factors influencing phenolic yield. The optimized conditions 50.79 % solvent concentration, 60 seconds extraction time, and 86.38 % ultrasonic amplitude resulted in a TPC of 106.42 ± 3.79 mg gallic acid equivalents (GAE) per gram of dry weight (DW), a TFC of 14.37 ± 0.51 mg quercetin equivalents (QE) per gram DW, and a DPPH antioxidant capacity of 3.11 ± 0.85 mg ascorbic acid equivalents (AE) per gram DW. The experimental results closely matched the model predictions, validating the optimization process. The methanolic extracts of Erica multiflora demonstrated significant potential for use in pharmaceuticals, nutraceuticals, and functional foods.

以保证提取物质量、减少溶剂用量、规范提取条件为主要目标，建立并优化超声辅助提取多花莲叶中酚类化合物的方法。采用Box-Behnken设计，在不同的溶剂浓度、提取时间和超声振幅下，共进行了15次提取。采用响应面法（RSM）和人工神经网络（ANN）对提取工艺参数对苯酚得率的影响进行了建模。两种模型对所有测量的响应都显示出很强的预测能力。在提取参数中，振幅和时间是影响苯酚得率的关键因素。 % 50.79溶剂浓度的优化条件,60 秒萃取时间,和86.38 %超声波振幅导致TPC 106.42 ±3.79  mg没食子酸当量(GAE)每克干重(DW),交通14.37 ±0.51  毫克每克DW槲皮素等价物(QE),和DPPH 抗氧化能力3.11±0.85  毫克每克DW抗坏血酸等价物(AE)。实验结果与模型预测结果吻合较好，验证了优化过程。多花莲的甲醇提取物在制药、营养保健品和功能食品中具有重要的应用潜力。

{"title":"Optimization of ultrasound phenolic extraction from Erica multiflora leaves using response surface methodology and artificial neural networks","authors":"Anfel Benmanseur , Mustapha Tacherfiout , Yacine Benguerba , Fatma Zahra Hab , Rechda Amel Tachour , Bachra Khettal , Rania Derguine , Mostapha Bachir-Bey , Abdelmalek Rezgui , Widad Sobhi","doi":"10.1016/j.jarmap.2025.100627","DOIUrl":"10.1016/j.jarmap.2025.100627","url":null,"abstract":"<div><div>This study aimed to develop and optimize an efficient ultrasound-assisted extraction method for phenolic compounds from <em>Erica multiflora</em> leaves, with the primary objectives of preserving extract quality, minimizing solvent usage, and standardizing extraction conditions. A total of 15 extractions were performed, varying solvent concentration, extraction time, and ultrasonic amplitude, using a Box-Behnken design. The effects of the extraction parameters on phenolic yield were modeled using response surface methodology (RSM) and artificial neural networks (ANN). Both models demonstrated strong predictive capabilities for all measured responses. Among the extraction parameters, amplitude and time were identified as key factors influencing phenolic yield. The optimized conditions 50.79 % solvent concentration, 60 seconds extraction time, and 86.38 % ultrasonic amplitude resulted in a TPC of 106.42 ± 3.79 mg gallic acid equivalents (GAE) per gram of dry weight (DW), a TFC of 14.37 ± 0.51 mg quercetin equivalents (QE) per gram DW, and a DPPH antioxidant capacity of 3.11 ± 0.85 mg ascorbic acid equivalents (AE) per gram DW. The experimental results closely matched the model predictions, validating the optimization process. The methanolic extracts of <em>Erica multiflora</em> demonstrated significant potential for use in pharmaceuticals, nutraceuticals, and functional foods.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"45 ","pages":"Article 100627"},"PeriodicalIF":3.8,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0