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Circular Economy Integration in 1G+2G Sugarcane Bioethanol Production: Application of Carbon Capture, Utilization and Storage, Closed-Loop Systems, and Waste Valorization for Sustainability 1G+2G 甘蔗生物乙醇生产中的循环经济整合:碳捕获、利用和储存、闭环系统和废物价值化在可持续发展中的应用
Q2 Engineering Pub Date : 2024-07-08 DOI: 10.14416/j.asep.2024.07.005
Rich Jhon Paul Latiza, R. V. Rubi
Bioethanol production is a vital player in the renewable energy landscape. However, it faces pressing issues regarding carbon emissions and resource management. Traditional open-loop systems generate substantial waste and pollution, exacerbating environmental concerns. Various emerging technologies offer promising solutions. Carbon Capture, Utilization, and Storage (CCUS) presents avenues for tackling carbon emissions. Utilization transforms CO2 emissions into valuable products, while Storage securely stores emissions to prevent atmospheric release. Closed-loop processes and waste valorization capitalize on material reuse, conserving natural resources, and minimizing waste. By promoting resource efficiency and waste minimization, circular economy principles align seamlessly with CCUS, closed-loop systems, and waste valorization. This study delves into utilizing Utilization technologies tailored to sugarcane 1G+2G bioethanol production, evaluates CO2 capture options, and presents applications. Storage strategies suitable for bioethanol production facilities are scrutinized, and deployment options are explored, highlighting the closed-loop system and waste valorization's role in waste reduction and environmental preservation. Through synergistic integration, these technologies pave the way for sustainable sugarcane bioethanol production, addressing economic and technological challenges while fostering innovation and collaboration. This comprehensive study will serve as a guide for transitioning to a circular economy model in bioethanol production.
生物乙醇生产是可再生能源领域的重要一员。然而,它面临着碳排放和资源管理方面的紧迫问题。传统的开环系统会产生大量废物和污染,加剧了环境问题。各种新兴技术提供了前景广阔的解决方案。碳捕集、利用和封存(CCUS)为解决碳排放问题提供了途径。利用 "将二氧化碳排放转化为有价值的产品,而 "储存 "则将排放物安全地储存起来,以防止排放到大气中。闭环工艺和废物价值化利用了材料再利用、自然资源保护和废物最小化。通过促进资源效率和废物最小化,循环经济原则与 CCUS、闭环系统和废物价值化完美结合。本研究深入探讨了适合甘蔗 1G+2G 生物乙醇生产的利用技术,评估了二氧化碳捕集方案,并介绍了相关应用。此外,还仔细研究了适合生物乙醇生产设施的储存策略,并探讨了部署方案,强调了闭环系统和废物价值化在减少废物和保护环境方面的作用。通过协同整合,这些技术为甘蔗生物乙醇的可持续生产铺平了道路,在应对经济和技术挑战的同时促进了创新与合作。这项综合研究将为生物乙醇生产向循环经济模式过渡提供指导。
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引用次数: 0
Inhibiting Stenotrophomonas maltophilia, a Pathogenic Bacterium Responsible for Kernel Rot Disease in Pili nut (Canarium ovatum Engl.) with Ionic Liquid-loaded Nanoemulsions 用离子液体负载纳米乳剂抑制嗜麦芽僵化单胞菌--一种导致纤果(Canarium ovatum Engl.)核腐烂病的致病细菌
Q2 Engineering Pub Date : 2024-07-05 DOI: 10.14416/j.asep.2024.07.004
Roberth San Abando Solita, Felmer S. Latayada, Julius Anthony Magadan Leones, Elizabeth P. Parac, A. Alguno, R. Capangpangan
Pili nut production in the Philippines has grown steadily, but it faces significant challenges from pests and diseases, notably kernel rot. Yield losses due to this pathogen are still not measured, but the damage could extend from the purple immature to the dried postharvest nuts. Therefore, there is a pressing need for safe, effective, and environmentally friendly control measures. This study reports on the successful formulation of various Ionic Liquid-loaded Eucalyptus Essential Oil Nanoemulsions (IL-EEONE) for potential applications against Stenotrophomonas maltophilia, a pathogenic bacterium responsible for kernel rot disease in Pili nut (Canarium ovatum). Combining eucalyptus essential oil (EO) and Tween 80 in an oil-in-water (O/W) system, followed by stirring and sonication, and the subsequent loading of 1-Butyl-3-methylimidazolium hydrogen sulfate ([Bmim][HSO4]), an ionic liquid, at varying ratios (1:1, 2:1, and 3:1), yielding the formation of IL-EEONE. The nanoemulsion droplets exhibited a size range of 9.4–12.26 nm, highlighting their nanoscale dimensions. The IL-loaded nanoemulsions formulated at varying ratios typically displayed nearly monodisperse characteristics, except for the higher concentration 1:1 ratio of IL:EEONE formulation, as indicated by their Polydispersity Index (PDI) values. Fourier Transform Infrared (FT-IR) analysis further confirmed the successful formulation of the different IL-EEONE nanoemulsion compositions. Significantly, these nanoemulsions demonstrated excellent inhibitory properties against S. maltophilia, as indicated by Zone of Inhibition (ZOI) ranging from 11.3 ± 0.58 mm to 32.7 ± 0.58 mm. The antibacterial activity varied from partially active to very active across different formulations, with the 1:1 IL-EEONE ratio formulation standing out as exceptionally effective. This study shows the potential of IL-loaded nanoemulsions, IL-EEONE, as a potential agent for mitigating S. maltophilia causing the kernel rot disease, offering innovative avenues for addressing bacterial infection in agricultural settings.
菲律宾的霹雳坚果产量稳步增长,但也面临着病虫害带来的巨大挑战,尤其是果仁腐烂病。这种病原体造成的产量损失仍无法估量,但其危害可能从紫色的未成熟坚果一直延伸到收获后的干果。因此,迫切需要安全、有效和环保的控制措施。本研究报告了各种离子液体负载桉树精油纳米乳剂(IL-EEONE)的成功配制,这些纳米乳剂可用于防治嗜麦芽僵化单胞菌(一种导致皮里坚果(Canarium ovatum)果核腐烂病的病原菌)。在水包油(O/W)体系中加入桉树精油(EO)和吐温 80,然后进行搅拌和超声处理,随后以不同比例(1:1、2:1 和 3:1)加入离子液体 1-丁基-3-甲基咪唑硫酸氢盐([Bmim][HSO4]),形成 IL-EEONE。纳米乳液液滴的尺寸范围为 9.4-12.26 纳米,凸显了其纳米级尺寸。除了浓度较高的 1:1 比例的 IL:EEONE 配方外,以不同比例配制的负载 IL 的纳米乳液通常显示出接近单分散的特性,这一点可以从它们的多分散指数(PDI)值看出。傅立叶变换红外(FT-IR)分析进一步证实了不同 IL-EEONE 纳米乳液组合物的成功配制。值得注意的是,这些纳米乳液对嗜麦芽糖酵母菌具有极佳的抑制特性,抑制区(ZOI)从 11.3 ± 0.58 mm 到 32.7 ± 0.58 mm 不等。不同配方的抗菌活性从部分活性到非常活性不等,其中 1:1 IL-EEONE 比率配方的抗菌效果尤为突出。这项研究表明,载入 IL 的纳米乳剂 IL-EEONE 具有减轻嗜麦芽酵母菌引起核腐病的潜在作用,为解决农业环境中的细菌感染问题提供了创新途径。
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引用次数: 0
Bio-sourced Black Soldier Fly (Hermetia illucens) Maggot Chitosan/PVA/PAN-based Polymer Electrolyte Membrane for Sustainable Energy Storage Applications 基于壳聚糖/PVA/PAN 的生物源黑兵蝇(Hermetia illucens)蛆聚合物电解质膜在可持续能源存储应用中的应用
Q2 Engineering Pub Date : 2024-07-04 DOI: 10.14416/j.asep.2024.07.002
Muhammad Thoriq Al Fath, N. F. Dalimunthe, Rivaldi Sidabutar, Michael Michael, Rosma Natalia Samosir, Thiodorus Marvin Tjandra, Gina Cynthia Raphita Hasibuan
The global energy crisis sparked by dwindling fossil fuel reserves has precipitated efforts to develop sustainable battery technologies, as conventional dry cell batteries utilize toxic lead, graphite, and manganese oxide components that pollute the environment. Chitosan derived from black soldier fly (Hermetia illucens) maggot presents a biodegradable substitute. This study fabricated chitosan-based polymer electrolyte membranes by blending chitosan with polyvinyl alcohol (PVA) and polyacrylonitrile (PAN), then doping with ammonium chloride (NH4Cl) using the solvent-casting method. Varying NH4Cl compositions aimed to maximize ionic conductivity. Chitosan (13.455% water, 27.810% ash) was subsequently combined with PVA/PAN (20:80 w/w), NH4Cl, and casted onto petri dishes. Electrolyte membranes exhibited a maximum conductivity of 0.19612 ± 0.01572 S/cm with 0.9 g NH4Cl. FTIR spectroscopy verified the incorporation of chitosan (peaks at 3446.79 cm–1, 1643.35 cm–1, and 1151.50 cm–1), PVA (3446.79 cm–1 and 1136.07 cm–1), and NH4Cl (3371.57 cm–1 and 721.38 cm–1). SEM imaging visualized the incorporation of NH4Cl within the membrane. The chitosan-based biodegradable approach is compelling but limited by 0.19612 S/cm ionic conductivity, necessitating further compositional and processing optimizations for viable applications. Though it is promising for sustainable bio-sourced energy storage, challenges remain in enhancing conductivity through advanced polymer blends/dopants and scaling up for commercial biobattery manufacturing.
化石燃料储量不断减少引发的全球能源危机促使人们努力开发可持续电池技术,因为传统干电池使用的有毒铅、石墨和氧化锰成分会污染环境。从黑兵蝇(Hermetia illucens)蛆中提取的壳聚糖是一种可生物降解的替代品。本研究将壳聚糖与聚乙烯醇(PVA)和聚丙烯腈(PAN)混合,然后用溶剂浇注法掺入氯化铵(NH4Cl),制成了壳聚糖基聚合物电解质膜。不同的 NH4Cl 成分旨在最大限度地提高离子传导性。壳聚糖(含水 13.455%,灰分 27.810%)随后与 PVA/PAN(20:80 w/w)和 NH4Cl 混合,并浇铸到培养皿上。在 0.9 g NH4Cl 的条件下,电解质膜的最大电导率为 0.19612 ± 0.01572 S/cm。傅立叶变换红外光谱验证了壳聚糖(峰值为 3446.79 cm-1、1643.35 cm-1 和 1151.50 cm-1)、PVA(3446.79 cm-1 和 1136.07 cm-1)和 NH4Cl(3371.57 cm-1 和 721.38 cm-1)的加入。扫描电子显微镜成像显示了 NH4Cl 在膜中的结合情况。基于壳聚糖的可生物降解方法很有吸引力,但受限于 0.19612 S/cm 的离子传导性,有必要进一步优化成分和加工,以实现可行的应用。虽然这种方法有望实现可持续的生物能源存储,但在通过先进的聚合物混合物/掺杂剂提高导电性以及扩大商业生物电池制造规模方面仍存在挑战。
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引用次数: 0
Research Progress on using Omics Technology to Examine the Antimicrobial Mechanisms of Natural Active Substances 利用 Omics 技术研究天然活性物质抗菌机制的研究进展
Q2 Engineering Pub Date : 2024-07-04 DOI: 10.14416/j.asep.2024.07.003
Chaoyi Zeng, Atittaya Tandhanskul, Samatcha Krungkaew, Tanawan Likhanapaiboon, Witthawat Kasayapanan, P. Yasurin, Jie Tang, T. Phusantisampan, A. Tawai
Pathogenic microbial metabolism during food storage can lead to food spoilage, which can cause food poisoning and foodborne infections, posing a significant risk to human health and safety. Additionally, food spoilage generates greenhouse gases, which could contribute to global warming and have significant impacts. These challenges prompt us to explore effective solutions to reduce food spoilage, aiming to mitigate its adverse impacts. Many secondary plant metabolites have been used in the food and pharmaceutical industries due to their natural antimicrobial activity and low drug resistance. However, the reported targets of antibacterial action are complex, and with the continuous development of research methods, it has become possible to deeply analyze the antibacterial mechanisms using omics technologies. This article discussed the trends and application of transcriptomics, metabolomics, and proteomics in investigating the antimicrobial and antifungal properties of essential oils (EOs) and their active ingredients, aiming to provide a theoretical basis for the use of plant EOs and their active ingredients in addressing health risks and environmental challenges posed by food spoilage.
食品储存过程中的病原微生物新陈代谢会导致食品变质,从而引起食物中毒和食源性感染,对人类健康和安全构成重大风险。此外,食品腐败还会产生温室气体,导致全球变暖并产生重大影响。这些挑战促使我们探索减少食品腐败的有效解决方案,以减轻其不利影响。由于具有天然抗菌活性和低耐药性,许多植物次生代谢物已被用于食品和制药行业。然而,所报道的抗菌作用靶点十分复杂,随着研究方法的不断发展,利用全息技术深入分析抗菌机制已成为可能。本文讨论了转录组学、代谢组学和蛋白质组学在研究精油及其活性成分的抗菌和抗真菌特性方面的趋势和应用,旨在为利用植物精油及其活性成分应对食品腐败带来的健康风险和环境挑战提供理论依据。
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引用次数: 0
Phenol Removal through Horseradish Peroxidase Immobilization on Ultrafiltration Membranes: Comparative Analysis of Immobilization Methods and Fouling Patterns 通过在超滤膜上固定辣根过氧化物酶去除苯酚:固定化方法和堵塞模式的比较分析
Q2 Engineering Pub Date : 2024-07-02 DOI: 10.14416/j.asep.2024.07.001
Apinya Onsarn, Karnika Karnika, S. Mattaraj, Wipada Dechapanya, Tiammanee Rattanaweerapan, Sompop Sanongraj
This research investigates the removal of phenol using pure peroxidase from horseradish grade I in conjunction with a dead-end ultrafiltration membrane. Various horseradish peroxidase (HRP) immobilization techniques— physical adsorption, covalent bonding, and cross-linking with glutaraldehyde—were applied to a regenerated cellulose (RC) membrane with a surface area of 44 m2 and a molecular weight cut-off of 30 kDa. The investigation examined factors influencing phenol removal, including phenol concentration, membrane fouling, and the reusability of immobilized enzymes. Results indicated that covalent bonding was the most suitable enzyme immobilization technique, achieving a remarkable 90.1% immobilization yield. Phenol removal efficiency reached 100% at 30 min under specific conditions: phenol concentration of 1 mg/L, pH 6.0, hydrogen peroxide concentration of 0.5 mM, and operating pressure set at 3 psig, with temperature maintained at 28 ± 3 °C. Membrane fouling resulted in a decrease in flux. The performance of fouling models was found to be influenced by phenol concentration, with the Cake Formation Model (CFM) proving most effective at low concentrations, while the Complete Pore Blocking Model (CBM) emerged as more suitable at higher concentrations. The immobilized enzyme exhibited reusability for five cycles, maintaining a phenol removal efficiency exceeding 50%. These findings contribute to understanding the enzymatic phenol removal process and the use of appropriate enzyme immobilization techniques for the effective and sustainable treatment of phenol-contaminated water.
本研究调查了使用 I 级辣根纯过氧化物酶结合死端超滤膜去除苯酚的情况。将各种辣根过氧化物酶(HRP)固定化技术--物理吸附、共价键和戊二醛交联--应用于表面积为 44 平方米、截留分子量为 30 kDa 的再生纤维素(RC)膜。调查研究了影响苯酚去除的因素,包括苯酚浓度、膜堵塞和固定化酶的可重复使用性。结果表明,共价键是最合适的酶固定化技术,固定化率高达 90.1%。在特定条件下:苯酚浓度为 1 mg/L,pH 值为 6.0,过氧化氢浓度为 0.5 mM,操作压力为 3 psig,温度保持在 28 ± 3 °C,30 分钟后苯酚去除率达到 100%。膜堵塞导致通量下降。污垢模型的性能受苯酚浓度的影响,在低浓度下,结块模型(CFM)最有效,而在高浓度下,完全孔堵塞模型(CBM)更适用。固定化酶可重复使用五个周期,苯酚去除率超过 50%。这些发现有助于了解酶法除酚过程,并有助于使用适当的酶固定化技术有效、可持续地处理苯酚污染的水。
{"title":"Phenol Removal through Horseradish Peroxidase Immobilization on Ultrafiltration Membranes: Comparative Analysis of Immobilization Methods and Fouling Patterns","authors":"Apinya Onsarn, Karnika Karnika, S. Mattaraj, Wipada Dechapanya, Tiammanee Rattanaweerapan, Sompop Sanongraj","doi":"10.14416/j.asep.2024.07.001","DOIUrl":"https://doi.org/10.14416/j.asep.2024.07.001","url":null,"abstract":"This research investigates the removal of phenol using pure peroxidase from horseradish grade I in conjunction with a dead-end ultrafiltration membrane. Various horseradish peroxidase (HRP) immobilization techniques— physical adsorption, covalent bonding, and cross-linking with glutaraldehyde—were applied to a regenerated cellulose (RC) membrane with a surface area of 44 m2 and a molecular weight cut-off of 30 kDa. The investigation examined factors influencing phenol removal, including phenol concentration, membrane fouling, and the reusability of immobilized enzymes. Results indicated that covalent bonding was the most suitable enzyme immobilization technique, achieving a remarkable 90.1% immobilization yield. Phenol removal efficiency reached 100% at 30 min under specific conditions: phenol concentration of 1 mg/L, pH 6.0, hydrogen peroxide concentration of 0.5 mM, and operating pressure set at 3 psig, with temperature maintained at 28 ± 3 °C. Membrane fouling resulted in a decrease in flux. The performance of fouling models was found to be influenced by phenol concentration, with the Cake Formation Model (CFM) proving most effective at low concentrations, while the Complete Pore Blocking Model (CBM) emerged as more suitable at higher concentrations. The immobilized enzyme exhibited reusability for five cycles, maintaining a phenol removal efficiency exceeding 50%. These findings contribute to understanding the enzymatic phenol removal process and the use of appropriate enzyme immobilization techniques for the effective and sustainable treatment of phenol-contaminated water.","PeriodicalId":8097,"journal":{"name":"Applied Science and Engineering Progress","volume":"5 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141684323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development and Characterization of Hybrid Particulate-fiber Reinforced Epoxy Composites 混合微粒-纤维增强环氧树脂复合材料的开发与表征
Q2 Engineering Pub Date : 2024-06-04 DOI: 10.14416/j.asep.2024.06.001
A. Akinwekomi, I. Oladele, Li Onuh, Essien Essien Essien, N. I. Agbeboh, M. Idris
Although considered wastes, animal fibers and gastropod shell particles are biodegradable, have low density, high stiffness, considerably high impact absorption capacity and relatively low cost. Therefore, they are finding increasing use as reinforcement materials in polymer composites. This research work studied the tensile, hardness, and wear resistance properties of hybrid snail shell (SSP) and chicken feather barb fibers (CFB) reinforced epoxy composites. The stir cast molding technique was utilized to synthesize the composite samples with 3, 6, 9, 12, 15, and 18 wt.% of the hybrid SSPs/CFB. Compared with the control samples, SSP/CFB hybrid reinforcements enhanced the mechanical properties of the composites. Composites with intermediate weight fraction of 9 wt.% SSP/CFB exhibited overall optimum properties when benchmarked against the control sample with approximately 37, 37, 133, 19, and 59% improvement in wear, hardness, impact, and ultimate tensile strength properties respectively. These enhancements suggested a synergistic effect of the two reinforcement phases. The results presented in this study demonstrated the potential of utilizing bio-derived waste materials for synthesizing eco-friendly composites.
动物纤维和腹足类贝壳颗粒虽然被视为废物,但它们可生物降解、密度低、刚度高、冲击吸收能力相当强,而且成本相对较低。因此,它们越来越多地被用作聚合物复合材料的增强材料。本研究对混合蜗牛壳(SSP)和鸡毛钩纤维(CFB)增强环氧树脂复合材料的拉伸、硬度和耐磨性能进行了研究。采用搅拌铸造成型技术合成了重量百分比分别为 3、6、9、12、15 和 18 的混合 SSP/CFB 复合材料样品。与对照样品相比,SSP/CFB 混合增强材料提高了复合材料的机械性能。与对照样品相比,中间重量百分比为 9 wt.% 的 SSP/CFB 复合材料表现出最佳的整体性能,在磨损、硬度、冲击和极限拉伸强度性能方面分别提高了约 37%、37%、133%、19% 和 59%。这些改进表明两种强化相具有协同效应。本研究的结果证明了利用生物衍生废料合成环保复合材料的潜力。
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引用次数: 0
Design of Machine Learning for Limes Classification Based Upon Thai Agricultural Standard No. TAS 27-2017 基于泰国农业标准第 TAS 27-2017 号的莱姆分类机器学习设计
Q2 Engineering Pub Date : 2024-01-26 DOI: 10.14416/j.asep.2024.01.005
A. Kengpol, Alongkorn Klaiklueng
Accurately classifying the limes quality of limes according to established standards is paramount for instilling trust in farmers' trading of agricultural produce. Historically, machinery has been employed to categorize the lime quality, with dual objectives of cost reduction and error mitigation, thereby facilitating the classifying process. Nevertheless, deploying such machinery to classify limes in their fresh produce form, intended for consumer sale, has encountered limitations imposed by the stringent criteria stipulated in Thai Agricultural Standards No. TAS 27-2017, a standard derived from the Codex Standard and widely adopted by numerous countries. Considering these constraints, the presented research aims to enhance the efficiency of limes classification, adhering to the standards. The Machine Learning System is designed to recognize and categorize limes based upon their skin color and defects to achieve this goal. This system employed convolutional neural network (CNN) models in conjunction with logistic regression equations, which are unavailable in the literature. The research findings indicate that this system is proficient in accurately presenting lime images and their corresponding quality classes via a Graphical User Interface on a computer screen, achieving an accuracy rate exceeding 90%. The implications of this research extend to the agricultural sector by augmenting the efficacy of Machine Learning for classifying limes in compliance with Thai Agricultural Standard No. TAS 27-2017. Furthermore, the methodology developed in this study can find applicability in classifying other agricultural products.
按照既定标准对石灰的质量进行准确分类,对于建立农民对农产品交易的信任至关重要。从历史上看,人们一直使用机械对酸橙质量进行分类,以达到降低成本和减少误差的双重目的,从而促进分类过程。然而,使用这种机械对供消费者销售的新鲜酸橙进行分类,却遇到了泰国农业标准第 TAS 27-2017 号规定的严格标准所带来的限制,该标准源自食品法典标准,被许多国家广泛采用。考虑到这些限制因素,本研究旨在根据标准提高酸橙分类的效率。为实现这一目标,设计了机器学习系统,根据青柠檬的肤色和缺陷对其进行识别和分类。该系统采用了卷积神经网络(CNN)模型和逻辑回归方程,这在文献中是没有的。研究结果表明,该系统能够通过计算机屏幕上的图形用户界面准确呈现石灰图像及其相应的质量等级,准确率超过 90%。这项研究的意义延伸到了农业领域,它增强了机器学习在按照泰国农业标准第 TAS 27-2017 号对石灰进行分类方面的功效。此外,本研究开发的方法还适用于其他农产品的分类。
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引用次数: 0
Recent Updates on Jellyfish: Applications in Agro-based Biotechnology and Pharmaceutical Interests 水母的最新情况:在农业生物技术和制药领域的应用
Q2 Engineering Pub Date : 2024-01-24 DOI: 10.14416/j.asep.2024.01.004
N. Barzkar, B. Thumthanaruk, M. S. Kalhoro, V. Rungsardthong, T. Phusantisampan
Jellyfish are gelatinous sea creatures that belong to the subphylum Medusozoa of the phylum Cnidaria and are found on many beaches worldwide. Despite being considered a nuisance, jellyfish have many uses, such as being a source of high-value molecules such as collagen, gelatin, and protein hydrolysates and a source of high-protein food. Studies related to its availability, post-harvest applications, and need-based use in biomedicine are thrust research of analysis or investigation. Therefore, this review has been designed with all the latest information with a focus on applications of jellyfish in agro-based biotechnology and pharmaceutics. The review has been systematically arranged to present on the broader search platform for future research studies and possible need-based applications.
水母是一种胶状的海洋生物,属于腔肠动物门的中生动物亚门,在世界各地的许多海滩上都有发现。尽管水母被认为是一种讨厌的生物,但它有许多用途,例如是胶原蛋白、明胶和蛋白质水解物等高价值分子的来源,也是高蛋白食物的来源。与海蜇的可用性、收获后的应用以及在生物医学中的需求相关的研究是分析或调查的重点。因此,本综述汇集了所有最新信息,重点关注海蜇在农业生物技术和制药方面的应用。这篇综述经过系统地编排,为未来的研究和可能的应用提供了更广阔的搜索平台。
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引用次数: 0
Diversity of Anammox Bacteria from Landfill Treatment Plant Sludge in Tropical Area 热带地区垃圾填埋处理厂污泥中厌氧菌的多样性
Q2 Engineering Pub Date : 2024-01-18 DOI: 10.14416/j.asep.2024.01.003
Z. Zulkarnaini, Nadiah Atsil, Hillary Citra Aribah, P. S. Komala, Shinta Silvia Shinta Silvia, Norihisa Matsuura
The anaerobic ammonium oxidation (anammox) process is known as the warm process. Tropical areas have an advantage due to their consistent temperature throughout the year. This study analyzed the diversity of anammox bacteria in the tropical area using leachate sludge from a landfill as an inoculum in a filter bioreactor (FtBR) and observed nitrogen removal performance. Ammonium and nitrite concentrations of 70, 150, and 200 mg-N/L were delivered into the reactor continuously with hydraulic retention time (HRT) of 24 h and 12 h and run for 131 days at ambient tropical temperature (25–28 °C). High performance achieved with nitrogen removal rate (NRR), nitrogen removal efficiency (NRE), and ammonium conversion efficiency (ACE) were 0.866 kg-N/m3.d, 99.19%, and 98.90%, respectively. The cultivated leachate sludge could perform an anammox process with four anammox species, Candidatus Brocadia fulgida, Candidatus Brocadia sapporoensis, Candidatus Brocadia sp uncultured, Candidatus Jettenia sp with abundance 6.52%, 13.82%, 0.77%, and 0.69%, respectively. These findings contribute to the advancement of biotechnology in wastewater treatment, particularly in tropical countries, and highlight the potential for highly cost-effective technology.
厌氧氨氧化(anammox)过程被称为温暖过程。热带地区因全年温度稳定而具有优势。本研究使用垃圾填埋场的渗滤液污泥作为过滤生物反应器(FtBR)中的接种物,分析了热带地区厌氧氨氧化细菌的多样性,并观察了脱氮性能。在热带环境温度(25-28 °C)下,向反应器连续输送 70、150 和 200 mg-N/L 浓度的铵和亚硝酸盐,水力停留时间(HRT)分别为 24 小时和 12 小时,运行 131 天。所获得的高性能脱氮率(NRR)、脱氮效率(NRE)和氨转化效率(ACE)分别为 0.866 kg-N/m3.d、99.19% 和 98.90%。经培养的渗滤液污泥可进行氨氧化过程,其中有四种氨氧化物种:Candidatus Brocadia fulgida、Candidatus Brocadia sapporoensis、Candidatus Brocadia sp uncultured、Candidatus Jettenia sp,丰度分别为 6.52%、13.82%、0.77% 和 0.69%。这些发现有助于推动废水处理生物技术的发展,特别是在热带国家,并凸显了高成本效益技术的潜力。
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引用次数: 0
Enzyme-Assisted Extraction of Fucosylated Chondroitin Sulfate from Sea Cucumber Holothuria scabra and Bohadschia argus and their Potential in Pharmaceutical Applications 酶辅助提取海参 Holothuria scabra 和 Bohadschia argus 中的岩藻糖基化硫酸软骨素及其在药物中的应用潜力
Q2 Engineering Pub Date : 2023-12-15 DOI: 10.14416/j.asep.2023.12.004
K. Rattanaporn, Thanagorn Ruensodsai, Richard Q. Mensah, Shrivarshini Vasudevan, Ratheeshkumar Shanmugam, P. Venkatachalam, Nichaphat Kitiborwornkul, M. Sriariyanun
Due to the health benefit of fucosylated chondroitin sulfate (FuCS), the efficient method for extraction of FuCS from raw materials is a crucial issue in reducing the production cost. In this study, enzymatic extraction of FuCS from two species of sea cucumber, Holothuria scabra and Bohadschia argus was undertaken using two protease enzymes, alcalase and papain. Response surface methodology (RSM) was employed in determining the optimal extraction conditions with the highest yield of FuCS concentration. The predicted optimal papain-assisted extraction conditions of Holothuria scabra and Bohadschia argus obtained a predicted FuCS yield of 1609.73 mg/100 g and 444.51 mg/100 g, respectively. To compare extraction efficiencies of two protease enzymes, employment of the RSM optimal conditions to Holothuria scabra resulted in 1538.76 ± 20.26 mg/ 100 g and 1295.50 ± 14.28 mg/100 g of purified FuCS for papain and alcalase, respectively. Whereas Bohadschia argus produced 412.39 ± 10.12 mg/100 g and 461.11 ± 8.45 mg/100 g purified FuCS for papain and alcalase, respectively. The acquired FTIR and NMR spectrums of extracted FuCS showed typical bands of sulfation patterns and were compared to commercial FuCS. The extracted FuCS showed enzyme type dependent antioxidant activity, and significant tyrosinase inhibitory activity than commercial FuCS. It also exhibited similar anti-glucosidase activity as commercial FuCS. Thus, this study reveals potential applications of enzyme-assisted FuCS from sea cucumber in food and pharmaceutical industries.
由于岩藻糖基化硫酸软骨素(FuCS)对健康有益,从原材料中提取 FuCS 的有效方法是降低生产成本的关键问题。本研究使用两种蛋白酶,即炼糖酶和木瓜蛋白酶,从两种海参,即疣刺海参(Holothuria scabra)和刺参(Bohadschia argus)中酶解提取 FuCS。采用响应面方法(RSM)确定了提取 FuCS 浓度产量最高的最佳提取条件。根据预测的最佳木瓜蛋白酶辅助提取条件,Holothuria scabra 和 Bohadschia argus 的 FuCS 产量分别为 1609.73 毫克/100 克和 444.51 毫克/100 克。为了比较两种蛋白酶的提取效率,对疮痂藻采用 RSM 最佳条件后,木瓜蛋白酶和脂肪酶的纯化 FuCS 产量分别为 1538.76 ± 20.26 mg/100 g 和 1295.50 ± 14.28 mg/100g。而 Bohadschia argus 产生的木瓜蛋白酶和脂肪酶纯化 FuCS 分别为 412.39 ± 10.12 mg/100 g 和 461.11 ± 8.45 mg/100g。提取的 FuCS 的傅立叶变换红外光谱和核磁共振光谱显示了硫酸化模式的典型条带,并与商用 FuCS 进行了比较。提取的 FuCS 表现出与酶类型相关的抗氧化活性,对酪氨酸酶的抑制活性明显高于商用 FuCS。它还表现出与商业 FuCS 相似的抗葡萄糖苷酶活性。因此,这项研究揭示了从海参中提取酶促 FuCS 在食品和制药业中的潜在应用。
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Applied Science and Engineering Progress
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