农林科学最新文献

{"title":"Transcriptomic and cellular responses to salinity stress in two populations of Asian clam (Corbicula fluminea) from estuarine and freshwater habitats","authors":"Yu Wang ,&nbsp;Yue Min ,&nbsp;Jianqi Yang ,&nbsp;Tianyu Yan ,&nbsp;Shikai Liu ,&nbsp;Qi Li","doi":"10.1016/j.aquaculture.2025.742937","DOIUrl":"10.1016/j.aquaculture.2025.742937","url":null,"abstract":"<div><div>Freshwater shortages and the growing demand for sustainable aquaculture have highlighted the potential of euryhaline species for brackish water and estuarine aquaculture. Asian clam (<em>Corbicula fluminea</em>) serves as a commercially valuable bivalve in East Asia. However, the physiological and molecular mechanisms underlying their salinity adaptation remain poorly understood. In this study, we investigated the transcriptomic and cellular responses of two Asian clam populations (estuarine and freshwater) exposed to salinities of 0, 15, and 25 PSU for 12 h, 24 h, and 5 days. A total of 50 RNA-Seq libraries (25 per population) were generated, revealing population-specific transcriptional reprogramming. The estuarine population exhibited a more tightly regulated and pathway-specific response, particularly under prolonged high-salinity exposure, with significant enrichment in MAPK and NF-κB signaling, amino acid metabolism, and osmoregulatory processes. In contrast, the freshwater population showed a broader yet less targeted transcriptomic profile. Weighted gene co-expression network analysis (WGCNA) identified key gene modules and hub genes associated with salinity stress, highlighting divergent regulatory strategies between populations. Histological and ultrastructural analyses of gill tissues corroborated transcriptomic findings, revealing more severe tissue damage in the freshwater population under high salinity, whereas the estuarine population maintained structural integrity. These results provide comprehensive insights into the molecular basis of salinity adaptation in Asian clam, offering valuable guidance for selective breeding programs aimed at enhancing salinity tolerance, expanding aquaculture potential, and improving environmental resilience in Asian clam.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742937"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144656913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Socio-economic opportunities and challenges of seaweed (Gracilaria sp.) farming in polyculture systems in Brebes regency, Indonesia","authors":"Olivia Azhari ,&nbsp;Sinne van der Veer ,&nbsp;Katell G. Hamon ,&nbsp;Ingvild H.T. Harkes ,&nbsp;Restiana W. Ariyati ,&nbsp;Lestari Lakhsmi ,&nbsp;Harxylen Kinanti Purnomo ,&nbsp;Sri Rejeki ,&nbsp;Lailatul Rokhmah ,&nbsp;W.K. van den Burg Sander","doi":"10.1016/j.aquaculture.2025.742944","DOIUrl":"10.1016/j.aquaculture.2025.742944","url":null,"abstract":"<div><div>Indonesia accounts for nearly 30 % of seaweed production worldwide and is one of the primary global producers. Part of Indonesian production is done in polyculture, defined as the joint cultivation of two or more species. This can improve pond productivity and increase environmental quality in the pond. Despite the potential benefits of polyculture, the socio-economic elements of the polyculture practices remain largely understudied. Investigated by means of literature review, interviews and survey of 101 farmers, this article aims to better understand the socio-economic aspects of polyculture seaweed (<em>Gracilaria</em> sp.) farming in Brebes regency, Central Java Province, Indonesia. The results presented illustrate that – from a financial perspective – there is no obvious incentive for farmers to switch to polyculture as there is no significant impact on operating profit. Two key considerations that influence adoption of polyculture practices are the relationship to other household productive activities and the benefits of polyculture. From societal perspective, polyculture is an alternative supporting farmers food resilience and offering the possibility to generate higher revenues per m². Farmers and farmer organizations need support for adopting polyculture practices. This can be done by increasing their bargaining power vis-à-vis the middlemen and/or financial support that enables farmers to do upfront investments in polyculture practices.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742944"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144656951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of P-AKK on glucose and lipid metabolism and immunity of Micropterus salmoides induced by high starch diet","authors":"Guokun Yang ,&nbsp;Boya Yang ,&nbsp;Tianhao Tan ,&nbsp;Mingyue Yin ,&nbsp;Jianing Gu ,&nbsp;Ying Zhang ,&nbsp;Yanmin Zhang ,&nbsp;Xindang Zhang ,&nbsp;Xulu Chang ,&nbsp;Shikun Feng ,&nbsp;Xiaolin Meng","doi":"10.1016/j.aquaculture.2025.742898","DOIUrl":"10.1016/j.aquaculture.2025.742898","url":null,"abstract":"<div><div>As the cheapest energy substance, the carbohydrate exerts a protein-sparing effect in the fish feed. However, excessive dietary carbohydrate intake induces glucose and lipid metabolic dysregulation, intestinal inflammatory responses in teleost. The AKK has been reported that it takes part in regulating glucose and lipid metabolism, inflammatory responses in mammals. However, roles of AKK in teleost remains limited and requires further systematic investigation. In present study, largemouth bass (<em>Micropterus salmoides</em>) was selected as experimental model organism. Following an 8-week culture experiment, the condition factor, triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and glycosylated hemoglobin (GHb) were promoted, while high-density lipoprotein cholesterol (HDL<img>C) was lowered in high starch diet group. Moreover, lipid droplet and glycogen in liver were promoted in high starch diet group. However, the liver fat and glycogen and serum lipid were reduced in the P-AKK supplemental group. In the liver, the mRNA of <em>g-6-pase</em>, <em>fas</em>, and <em>acc-a</em> were significantly inhibited, while the levels of <em>pk</em> and <em>lpl</em> were markedly promoted in P-AKK supplemental group compared with high starch diet and control groups. In the intestinal tissue, the levels of <em>tnf-α</em>, <em>MyD88</em>, <em>il-8</em>, <em>caspase3</em> and <em>caspase9</em> were observably inhibited with P-AKK supplemented into diet. Moreover, the TGF-β and IL-10 protein levels were observably elevated, and the TNF-α protein level was dramatically reduced in P-AKK supplemental group compared with high starch diet and control groups. These results demonstrate that P-AKK plays the crucial part in regulating glucose and lipid metabolism and intestinal inflammatory response in largemouth bass.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742898"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144534693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective role of oligochitosan in intestinal homeostasis to Edwardsiella ictaluri in hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)","authors":"Lixin Ma,&nbsp;Zhilong Chen,&nbsp;Zhuojin He,&nbsp;Xiaomin Zheng,&nbsp;Yutao Li,&nbsp;Cuiyun Zhou,&nbsp;Li Lin,&nbsp;Fei Shi","doi":"10.1016/j.aquaculture.2025.742923","DOIUrl":"10.1016/j.aquaculture.2025.742923","url":null,"abstract":"<div><h3>Background</h3><div><em>Edwardsiella ictaluri</em> is a major pathogen that causes severe infections in fish. Oligochitosan has antioxidant and antibacterial properties, and can also regulate the immune system and intestinal flora.</div></div><div><h3>Aims</h3><div>In this study, we investigated the mechanisms of <em>E. ictaluri</em> infection in hybrid grouper and evaluated the effects of oligochitosan supplementation on intestinal health, gut microbiota, and metabolism.</div></div><div><h3>Methods</h3><div>In this study, we employed a combination of histopathology, TdT-mediated dUTP nick end labeling (TUNEL), biochemical analysis, 16S rRNA sequencing, fluorescence in situ hybridization (FISH), transcriptomics, and metabolomics.</div></div><div><h3>Results</h3><div><em>E. ictaluri</em> infection significantly altered goblet cell counts (<em>P</em> &lt; 0.05), apoptosis rates (<em>P</em> &lt; 0.01), increased villus height (<em>P</em> &lt; 0.05), and reduced muscularis thickness (<em>P</em> &lt; 0.01). Oligochitosan intervention effectively restored intestinal structure. Compared to controls, <em>E. ictaluri</em> elevated intestinal superoxide dismutase (SOD) (<em>P</em> &lt; 0.05) and amylase (AMS) activities (<em>P</em> &lt; 0.01), while oligochitosan significantly decreased catalase (CAT) activity relative to infected fish (<em>P</em> &lt; 0.01). The infection also increased bacterial load and disrupted microbiota composition, whereas oligochitosan promoted beneficial genera, including <em>Faecalibaculum</em> and <em>Bacteroides</em>. FISH analysis confirmed increased bacterial colonization post-infection (<em>P</em> &lt; 0.01), which was alleviated by oligochitosan (<em>P</em> &lt; 0.01). Transcriptomics revealed that <em>E. ictaluri</em> altered genes involved in fat absorption and TNF signaling, effects mitigated by oligochitosan. Metabolomics showed infection suppressed D-amino acid, taurine, and hypotaurine metabolism, reducing amino acid levels, while oligochitosan reversed these trends. Multi-omics correlation analyses indicated that oligochitosan modulated intestinal amino acid metabolism by enhancing probiotic-associated pathways.</div></div><div><h3>Conclusion</h3><div>Collectively, oligochitosan can alleviate <em>E. ictaluri</em> induced by enhancing intestinal tissue integrity and antioxidant defense, maintaining intestinal flora balance, and regulating amino acid metabolism.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742923"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144548366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing the spatial configuration of scallop suspended cultivation net cages by leveraging seston flux variables based on the lattice Boltzmann hydrodynamics","authors":"Gangqin Tu ,&nbsp;Haifei Liu ,&nbsp;Zhiming Ru ,&nbsp;Tao Sun ,&nbsp;Wei Yang ,&nbsp;Wei Huang ,&nbsp;Li Cheng","doi":"10.1016/j.aquaculture.2025.742863","DOIUrl":"10.1016/j.aquaculture.2025.742863","url":null,"abstract":"<div><div>Suspended scallop farming using cultivation net cages in long-line cultivation structures is a common aquaculture activity. However, cultivation net cages in scallop production lines form obstacles that block currents. The hydrodynamic characteristics of an individual cultivation net cage, as well as the distance between net cages, can influence natural current circulation, further affecting food distribution and solute transport in the scallop farming environment. One of the challenges in scallop farming is to configure farms to optimize total scallop production and individual scallop quality under different environmental conditions. In this study, the appropriate spatial configuration of scallop farms was evaluated through the seston flux distribution characteristics of individual cultivation net cages under different environmental current speeds. A lattice Boltzmann model was established and validated. Then, this model was used to simulate the flow field and seston concentration around a cultivation net cage. The minimum seston flux disturbance of the cultivation net cage is taken as the criterion of the net cage distance. The longitudinal distance of the net cages was determined based on an 80% recovery of seston flux and a transverse distance of less than 5% flux disturbance. The results indicated that the longitudinal distance between net cages decreases with an increasing background flow velocity, and the transverse distance between net cages has little correlation with the background flow velocity. Thus, this study has potential applicability to improve the reforming and planning aquacultural sites.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742863"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144548365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benefits of using an exogenous protease on the performance, digestibility, and sustainability in white-leg shrimp (L. vannamei) farming","authors":"Ester Santigosa ,&nbsp;Rui J.M. Rocha ,&nbsp;Jorge Dias","doi":"10.1016/j.aquaculture.2025.742854","DOIUrl":"10.1016/j.aquaculture.2025.742854","url":null,"abstract":"<div><div>The effect of graded supplemental doses of an exogenous protease on the growth performance, nutrient digestibility, and nitrogen excretion of white leg shrimp (<em>Litopenaeus vannamei</em>) was assessed: A basal diet formulation mimicking a commercial shrimp feed was supplemented with 0, 100, 200 and 300 mg/kg equivalent to 30,000, 60,000 and 90,000 NFP/kg (diets CTRL, PRO100, PRO200 and PRO300, respectively) of an exogenous protease. Quintuplicate groups of 65 shrimp (IBW = 0.97 ± 0.1 g) were fed one of the four diets. After 62 days of feeding, final body weight (FBW) and specific growth rate (SGR) were not significantly affected by dietary treatments. Shrimp fed PRO200 and PRO300 diets showed a significantly lower feed intake (FI) and feed conversion ratio (FCR) than those fed the CTRL and PRO100 diets. Inversely, shrimp fed the diets with the highest enzyme inclusion (PRO200 and PRO300) demonstrated a significantly higher protein efficacy ratio (PER) than those fed the CTRL and PRO100 diets. Shrimp fed the PRO200 diet benefited from a significantly higher whole-body protein retention than those fed the CTRL diet. Irrespective of the dose, protease supplementation led to a significant increase of dry matter and protein digestibility. At the exception of Isoleucine (Ile) and Leucine (Leu), protease supplementation significantly increased the digestibility of all other amino acids. Shrimp fed all the protease-supplemented diets showed significantly lower excretion of total ammonia nitrogen (TAN), than those fed the CTRL diet. Overall data supports a beneficial effect of supplemental protease in shrimp, supported by an increase of protein and amino acid digestibility, which resulted in a significant improvement of feed utilization criteria (FCR and PER) and a lower excretion of TAN into the water. To demonstrate full efficacy, future trials should target more stringent scenarios of dietary protein supply, including diets formulated with lower crude protein levels and/or protein sources of lower quality.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742854"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Energy modulation in Atlantic salmon: Oleic acid increases adipogenesis and energy deposition","authors":"Beichen Yang ,&nbsp;Matthew K. Jago ,&nbsp;Thomas S. Mock ,&nbsp;Giovanni M. Turchini ,&nbsp;Michael J. Salini ,&nbsp;Richard P. Smullen ,&nbsp;David S. Francis","doi":"10.1016/j.aquaculture.2025.742895","DOIUrl":"10.1016/j.aquaculture.2025.742895","url":null,"abstract":"<div><div>Due to climate change, water temperatures in Tasmania (Australia) summer are rising at 0.4 °C per decade, exceeding the upper thermal tolerance limits of Atlantic salmon, leading to reduced feed intake, increased metabolic rate, and energy loss. These suboptimal conditions affect growth performance and quality of the final products. Thus, higher energy storage in salmon tissue prior to summer has been envisaged as a strategy to minimise the detrimental effects of this culture phase. The objective of this study was to investigate if dietary oleic acid could increase energy storage in visceral adipose tissue of juvenile Atlantic salmon reared under pre-summer conditions (15 °C), primarily as triacylglycerols, and assess whether it was more effective than high-lipid diets for energy modulation. For this, four diets were formulated and manufactured with two different levels of oleic acid (high and low) and two different total lipid (high and low) and fed to Atlantic salmon (71.8 ± 0.20 g) for 124 days. The results showed Atlantic salmon fed high-lipid diets demonstrated higher growth performance compared to the low-lipid diets, with a significantly lower feed conversion ratio, higher digestible energy retention, and higher triacylglycerol concentration in the fillet. High-oleic acid diets resulted in significantly higher total lipid and triacylglycerol concentrations in visceral adipose tissue compared to low-oleic acid diets. This was achieved via adipocyte hyperplasia originated from the increased provision of the energy substrate, oleic acid, in visceral adipose tissue lipid for triacylglycerol synthesis. Diets containing low lipid and high oleic acid accumulated the highest metabolic energy in visceral adipose tissue and can be considered a viable energy modulation strategy for Atlantic salmon. However, further investigation is needed to understand the long-term effects of triacylglycerol accumulation on fish health to ensure commercial viability.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742895"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144513820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of ocean acidification on skeletal structures in gilthead sea bream (Sparus aurata): In vitro and in vivo studies","authors":"Inmaculada Rodríguez ,&nbsp;Isabel García-Pérez ,&nbsp;Nazanin Sadeghi ,&nbsp;Manel Montblanch ,&nbsp;Joaquim Gutiérrez ,&nbsp;Isabel Navarro ,&nbsp;Encarnación Capilla ,&nbsp;Daniel Garcia de la serrana","doi":"10.1016/j.aquaculture.2025.742919","DOIUrl":"10.1016/j.aquaculture.2025.742919","url":null,"abstract":"<div><div>Ocean acidification is considered a significant risk to aquaculture, as it may adversely affect the growth and development of aquatic organisms. The effect of ocean acidification has been shown to impair the growth and survival of fish and to increase otoliths calcification in certain species; however, its effects on bone mineralization remain not well studied. The objective of the present study was to examine the effects of seawater acidification on the skeletal mineralization of gilthead sea bream juveniles, and to assess the direct impact of distinct pH levels on bone-derived cells development. After 68 days of exposure to low pH, fish exhibited a significantly reduced specific growth rate and elevated plasma pH levels, which influenced electrolyte concentrations such as potassium. Moreover, fish exposed to low pH showed increased otoliths size but no differences in shape. In bone, a higher vertebral length/height ratio was also observed, accompanied by significantly reduced opacity and increased expression of the osteoblast and osteoclast markers, alkaline phosphatase (<em>alp</em>) and matrix metalloproteinase 9 (<em>mmp9</em>), respectively, suggesting an elevated rate of bone turnover although reduced mineralization. <em>In vitro</em>, osteoblasts exposed to a low extracellular pH for 30 days exhibited increased viability and mineralization compared to cells maintained at a plasma pH or an alkaline pH. Additionally, the pH level significantly influenced the expression of several extracellular matrix components and osteoblast markers supporting those observations. Overall, these findings underscore the threat that ocean acidification poses to aquaculture, particularly through its impact on skeletal mineralization in gilthead sea bream, and highlight the importance of identifying approaches to farming resilient fish.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742919"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144572280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploiting the untapped wild resources: Developing new diploid tetrasporophyte Kappaphycus alvarezii cultivars from sporelings of wild cystocarpic parental plants","authors":"Jonh Rey L. Gacura ,&nbsp;Lourie Ann R. Hinaloc ,&nbsp;Rachel N. Oguira ,&nbsp;Bienson Ceasar V. Narvarte ,&nbsp;Ronel T. Aguilar ,&nbsp;Emmanuel M. Mendoza ,&nbsp;Vicenta Z. Projimo ,&nbsp;Michael Y. Roleda","doi":"10.1016/j.aquaculture.2025.742916","DOIUrl":"10.1016/j.aquaculture.2025.742916","url":null,"abstract":"<div><div>Successive clonal propagation of commercially cultivated haplotypes of <em>Kappaphycus alvarezii</em> from a limited gene pool over decades has plausibly led to strain fatigue and loss of vigor. Henceforth, diversifying the genetic pool of cultivated <em>K. alvarezii</em> became imperative to reverse these trends, and reinvigorate the productivity of the seaweed farming industry. Here, we utilized the spores from wild cystocarpic <em>K. alvarezii</em> to develop new cultivars that may potentially exhibit resilience to various biotic and abiotic stressors. In this study, we investigated the physiological, and biochemical performances of selected next-generation tetrasporophytes from cystocarpic parental plants of <em>K. alvarezii</em>of novel haplotypes<em>,</em> here labeled [strain (haplotype)] as, TR-C5 (KALV-D4), TR-R3(KALV-D3), TR-S8(KALV-D6), cultivated in land-based and sea-based conditions, and compared their performance to the commercially cultivated Tambalang brown strain (commercially farmed haplotype KALV-A3). Growth among the novel strains cultivated in land-based nursery conditions did not significantly vary ranging from 4.19 % day⁻¹ to 5.78 % day⁻¹. However, significant growth variability among strains was observed when cultivated in sea-based conditions ranging from 0.85 % day⁻¹ to 4.47 % day⁻¹ during D30, and 3.18 % day⁻¹ to 5.26 % day⁻¹ by D60. Moreover, susceptibility to ice-ice disease syndrome showed to be strain-specific with TR-S8 demonstrating lesser susceptibility among the novel strains. Except for the total protein, the biochemistry significantly varied among strains and between different cultivation methods (land-based hatchery vs. in situ sea-based farming). Our results further support the importance of exploring the presence of a reservoir of unutilized wild genotypes that can be used in developing new cultivars with superior traits for future domestication.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742916"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144570473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The IolR regulon contributes to Aeromonas hydrophila virulence in channel catfish (Ictalurus punctatus)","authors":"Tingbi Xu ,&nbsp;Dong Yuhao ,&nbsp;Mohammad J. Hossain ,&nbsp;Damien S. Waits ,&nbsp;Shabarinath Srikumar ,&nbsp;Jeffery S. Terhune ,&nbsp;Charles M. Thurlow ,&nbsp;Joseph C. Newton ,&nbsp;Yongjie Liu ,&nbsp;Mark R. Liles ,&nbsp;Dawei Sun","doi":"10.1016/j.aquaculture.2025.742878","DOIUrl":"10.1016/j.aquaculture.2025.742878","url":null,"abstract":"<div><div>Hypervirulent <em>Aeromonas hydrophila</em> (vAh) causes devastating damage in aquaculture worldwide. All vAh strains utilize myo-inositol (MI) as a sole carbon source prompted the previous study of the MI pathway in virulence. A range of gene-deletion mutants in the MI pathway were investigated through <em>in vivo</em> fish challenges to identify the insignificant role of MI pathway in virulence. However, the unexpected virulence of iolR mutants suggested the importance of IolR regulon as the transcriptional regulator for virulence. This study discerned the role of the IolR regulons on the pathogenesis of vAh. The <em>A. hydrophila</em> ML09–119 <em>iolR</em> gene was mutated to obtain different <em>iolR</em> expression levels. The results showed that increasing IolR expression caused decreased mortality in channel catfish 6 h post challenge, suggesting the role of IolR in repressing virulence gene transcription. A transcriptome analysis was conducted for ML09–119 and mutants. The results indicated that multiple putative genes involved in MI metabolism, fimbrial biogenesis, cell envelope integrity, and horizontal gene transfer might have IolR-regulated transcription, with a palindromic sequence identified as the putative IolR binding site. Notably, when comparing phenotypes of <em>iolR</em> mutants of the channel catfish isolate ML09–119 and the carp isolate NJ-35, the regulation of biofilm formation and auto-aggregation were only observed for the carp isolate, which may reflect host-specific transcriptional regulation. Further study is necessary to understand the differences in the IolR regulon for carp and catfish isolates, as well as the contribution of each of the IolR-regulated genes to vAh pathogenesis.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742878"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}