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Thermophilic bacteria employ a contractile injection system in hot spring microbial mats. 嗜热细菌在温泉微生物垫中采用可收缩注射系统。
IF 1 1区 环境科学与生态学 Q1 ECOLOGY Pub Date : 2026-02-10 DOI: 10.1093/ismejo/wrag021
Vasil A Gaisin, Corina Hadjicharalambous, Izabela Mujakić, Cristian Villena-Alemany, Jiangning Li, Michal Koblížek, Martin Pilhofer

Bacterial contractile injection systems (CISs) are multiprotein complexes that facilitate the bacterial response to environmental factors or interactions with other organisms. Multiple novel CISs have been characterised in laboratory bacterial cultures recently; however, studying CISs in the context of the native microbial community remains challenging. Here, we present an approach to characterise a bioinformatically predicted CIS by directly analysing bacterial cells from their natural environment. Using cryo-focused ion beam milling and cryo-electron tomography (cryoET) imaging, guided by 16S rRNA gene amplicon sequencing, we discovered that thermophilic Chloroflexota bacteria produce intracellular CIS particles in a natural hot spring microbial mat. We then found a niche-specific production of CIS in the structured microbial community using an approach combining metagenomics, proteomics, and immunogold staining. Bioinformatic analysis and imaging revealed CISs in other extremophilic Chloroflexota and Deinococcota. This Chloroflexota/Deinococcota CIS lineage shows phylogenetic and structural similarity to previously described cytoplasmic CIS from Streptomyces and probably shares the same cytoplasmic mode of action. Our integrated environmental cryoET approach is suitable for discovering and characterising novel macromolecular complexes in environmental samples.

细菌收缩注射系统(CISs)是一种多蛋白复合物,可促进细菌对环境因素的反应或与其他生物的相互作用。最近在实验室细菌培养中发现了多种新型CISs;然而,在本地微生物群落的背景下研究CISs仍然具有挑战性。在这里,我们提出了一种通过直接分析细菌细胞的自然环境来表征生物信息学预测的CIS的方法。利用低温聚焦离子束切割和低温电子断层扫描(cryoET)成像,在16S rRNA基因扩增子测序的指导下,我们发现嗜热的Chloroflexota细菌在天然温泉微生物基质中产生细胞内CIS颗粒。然后,我们利用宏基因组学、蛋白质组学和免疫金染色相结合的方法,在结构微生物群落中发现了特定利基的CIS颗粒。生物信息学分析和影像学显示,其他嗜极动物氯氟藻和Deinococcota也存在CISs。这个氯氟藻/Deinococcota CIS谱系与先前描述的链霉菌细胞质CIS具有系统发育和结构相似性,并且可能具有相同的细胞质作用模式。我们的集成环境低温et方法适用于发现和表征环境样品中的新型大分子复合物。
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引用次数: 0
Why organisational diversity matters in a circular economy and society 为什么组织多样性在循环经济和社会中很重要
IF 13.2 1区 环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL Pub Date : 2026-02-10 DOI: 10.1016/j.resconrec.2026.108833
Wim Van Opstal, Nancy Bocken, Jan Brusselaers
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引用次数: 0
Corrigendum to "illuminated fulvic acid stimulates denitrification and As(III) immobilization in flooded paddy soils via an enhanced biophotoelectrochemical pathway" [Sci. Total Environ. 912 (2024), 169670]. “照明黄腐酸通过增强的生物光电化学途径刺激水淹稻田土壤的反硝化和As(III)固定化”的更正。环境科学学报,2016,32(2):444 - 446。
IF 8 1区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES Pub Date : 2026-02-10 Epub Date: 2026-01-24 DOI: 10.1016/j.scitotenv.2026.181448
Yanqiong Zeng, Honghui Wang, Jiehua Hu, Jing Zhang, Feng Wang, Tongyu Wang, Randy A Dahlgren, Hui Gao, Zheng Chen
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引用次数: 0
Acute exposure to high concentrations of polystyrene nanoparticles induces genotoxicity in Daphnia pulex. 急性暴露于高浓度聚苯乙烯纳米颗粒诱导水蚤遗传毒性。
IF 5.8 3区 环境科学与生态学 0 ENVIRONMENTAL SCIENCES Pub Date : 2026-02-10 DOI: 10.1007/s11356-026-37490-9
Thomas Nash, Paul Kay, Laura J Carter

Micro- and nanoplastics (MNPs) are known to detrimentally impact a wide range of aquatic species, inducing mortality, decreased growth, a reduction in offspring production and increase in reactive oxygen species in their tissues. However, the genotoxic impact of MNPs in freshwater organisms remains understudied. In the present study we investigate the genotoxic impact of acute exposure to polystyrene nanoparticles (PSNPs) in Daphnia pulex using the comet assay, alongside immobilisation rate and hopping frequency. Daphnids were exposed to 100 nm PSNPs for 24 and 48 h at concentrations between 10 and 200 mg/L. Immobilisation increased with PSNPs concentration and exposure time, while hopping frequency among surviving daphnids decreased at concentrations above 100 mg/L after 24 h, and above 50 mg/L after 48 h. Comet assay results showed increasing DNA damage with concentration and exposure time, with significant DNA damage after 24 h of exposure to 200 mg/L PSNPs, and after 48 h of exposure to 100 and 200 mg/L. These results suggest that exposure to MNPs increases daphnid mortality, impacts behaviour, and induces genotoxicity. The incorporation of the comet assay into freshwater monitoring methods alongside existing techniques could further understanding of the full impact of MNP pollution upon ecosystem health.

众所周知,微塑料和纳米塑料(MNPs)会对许多水生物种产生不利影响,导致死亡、生长减慢、后代产量减少以及组织中活性氧含量增加。然而,MNPs对淡水生物的遗传毒性影响仍未得到充分研究。在本研究中,我们研究了急性暴露于聚苯乙烯纳米颗粒(PSNPs)对水蚤的遗传毒性影响,使用彗星试验,以及固定率和跳跃频率。水蚤以10 ~ 200 mg/L的浓度暴露于100 nm的PSNPs中24 ~ 48 h。当PSNPs浓度大于100 mg/L、大于50 mg/L、大于48 h时,存活水蚤的跳跃频率随浓度和暴露时间的增加而降低。彗星试验结果显示,DNA损伤随暴露时间的增加而增加,暴露于200 mg/L、100和200 mg/L、24 h后DNA损伤显著。这些结果表明,接触MNPs会增加水蚤死亡率,影响行为,并诱发遗传毒性。将彗星试验与现有技术一起纳入淡水监测方法,可以进一步了解MNP污染对生态系统健康的全面影响。
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引用次数: 0
Enhanced degradation of n-propylbenzene and isopropylbenzene in seawater through microalga-microalga consortium: Combination screening, inoculation ratio, kinetics, and degradation mechanisms 微藻-微藻联合增强海水中正丙苯和异丙苯的降解:组合筛选、接种比、动力学和降解机制
IF 5.8 3区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES Pub Date : 2026-02-10 DOI: 10.1016/j.marpolbul.2026.119376
Wenjia Sun, Yumei Li, Fanping Meng, Houlei Li, Wenlu Li
Accidental spill of C9 aromatics into the sea leads to adverse effects on the marine ecosystem. Two propylbenzenes, n-propylbenzene (n-PBZ) and isopropylbenzene (i-PBZ), are the main components in C9 aromatics. Thus, this study attempted to utilize microalga-microalga consortium (MMC) to eliminate PBZs' pollution in seawater. Among the 10 species of candidate microalgae, two species, diatom Chaetoceros calcitrans MZB-1 and Cryptophyta Rhinomonas reticulata S6A were selected because of their higher removal efficiencies for both PBZs (5 mg/L). When their total inoculation density was 5 × 105 cells/mL and the initial density ratio (MZB-1: S6A) was 1:2, the constructed MMC showed the highest removal efficiencies of 79.1% for n-PBZ and 47.1% for i-PBZ within seven days, which were significantly higher than that using the single species. When MMC was exposed to n-PBZ or i-PBZ ranging from 0.5 to 20 mg/L, alga MZB-1 generally grew faster than alga S6A. Degradation of n-PBZ and i-PBZ by MMC followed a first-order reaction model with the degradation rate constants of 0.10–0.37/d and 0.06–0.14/d, respectively. In the presence of n-PBZ or i-PBZ, seven and three intermediates were identified, respectively, revealing that each PBZ first underwent alkyl side chain oxidation to form aromatic alcohols, followed by conversion to aromatic acids. Compared with the single microalga, the main intermediates from PBZ degradation by MMC were more abundant, which accounted for the better removal of PBZs by the microalga-microalga combination. This work provides the first evidence that MMC enhances PBZs elimination and will contribute to the bioremediation of PBZ-contaminated seawater.
C9芳烃意外泄漏入海会对海洋生态系统造成不利影响。正丙苯(n-PBZ)和异丙苯(i-PBZ)是C9芳烃的主要成分。因此,本研究试图利用微藻-微藻联合体(MMC)来消除海水中pbz的污染。在10种候选微藻中,硅藻Chaetoceros calcitrans MZB-1和隐藻Rhinomonas reticulata S6A因其对两种pbz (5 mg/L)的去除效率较高而被选中。当总接种密度为5 × 105个/mL,初始密度比(MZB-1: S6A)为1:2时,构建的MMC在7 d内对n-PBZ的去除率最高,为79.1%,对i-PBZ的去除率为47.1%,显著高于单一菌种。当n-PBZ和i-PBZ浓度在0.5 ~ 20 mg/L范围内时,MMC藻类MZB-1的生长速度普遍快于S6A。MMC降解n-PBZ和i-PBZ符合一级反应模型,降解速率常数分别为0.10 ~ 0.37/d和0.06 ~ 0.14/d。在n-PBZ或i-PBZ存在下,分别鉴定了7个和3个中间体,表明每个PBZ首先经过烷基侧链氧化形成芳香醇,然后转化为芳香酸。与单一微藻相比,MMC降解PBZ的主要中间体更丰富,这是微藻-微藻组合对PBZ去除效果更好的原因。本研究首次证明了MMC能促进pbz的消除,并有助于pbz污染海水的生物修复。
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引用次数: 0
Pollution and health risk assessment of drinking water sources within artisanal and small-scale gold mining areas: a case of Asankrangwa District in Ghana. 手工和小规模金矿区内饮用水源的污染和健康风险评估:以加纳Asankrangwa县为例。
IF 5.8 3区 环境科学与生态学 0 ENVIRONMENTAL SCIENCES Pub Date : 2026-02-10 DOI: 10.1007/s11356-026-37463-y
Eric Danso-Boateng, Ebenezer Adom, Prince Appiah Owusu, Roland Songotu Kabange

Artisanal and small-scale gold mining (ASGM) is common in many developing countries; while it contributes to their socio-economic development, it also causes environmental pollution. This study examined the impact of ASGM activities on the quality of drinking water sources in the Asankrangwa District in Ghana, in terms of water quality index, heavy metal pollution index models, and human health risks. The results of physicochemical parameters indicate that 40.0% of all water sources are unsuitable for consumption. Heavy metal pollution indices indicated that 45.0% of the water sources were extremely polluted, and 25.0% were moderately polluted. For instance, cadmium (Cd) concentrations across all water sources reached levels up to 23 times the WHO limit (3.0 × 10-3 mg/L). Additionally, mercury (Hg) concentrations in 75.0% of the water sources (2.0 × 10-3-7.2 × 10-1 mg/L) exceed the maximum contaminant limit (2.0 × 10-3 mg/L) by approximately 361 times. Streams, in particular, exhibit higher Hg levels than groundwaters. Analysis revealed that 90% of the water sources pose a significant carcinogenic health risk upon consumption, with lifetime cancer risk values exceeding 1.0 × 10-4. The findings revealed that the pollution level is reduced and water quality improved 5 km from the ASGM sites; however, the water sources require treatment to render them suitable for consumption.

手工和小规模金矿开采在许多发展中国家很常见;在促进社会经济发展的同时,也造成了环境污染。本研究从水质指数、重金属污染指数模型和人类健康风险等方面考察了Asankrangwa地区农业农业活动对饮用水源质量的影响。理化参数分析结果表明,40.0%的水源不适宜饮用。重金属污染指标显示,45.0%的水源重度污染,25.0%的水源中度污染。例如,所有水源的镉(Cd)浓度达到世卫组织限值(3.0 × 10-3毫克/升)的23倍。此外,75.0%的水源中汞(Hg)浓度(2.0 × 10-3-7.2 × 10-1 mg/L)超过最大污染物限值(2.0 × 10-3 mg/L)约361倍。特别是溪流,其汞含量高于地下水。分析表明,90%的水源在饮用后存在显著的致癌健康风险,终生致癌风险值超过1.0 × 10-4。研究结果显示,在距ASGM场址5公里处,污染程度有所降低,水质有所改善;但是,需要对水源进行处理,使其适合消费。
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引用次数: 0
Evaluation of the ivermectin effects on porcine granulosa cell function 伊维菌素对猪颗粒细胞功能影响的评价
IF 4.3 3区 环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES Pub Date : 2026-02-10 DOI: 10.1016/j.etap.2026.104960
Giuseppina Basini, Fausto Quintavalla, Simona Bussolati, Francesca Grasselli
Ivermectin (IVM) is used antiparasitic drug in veterinary medicine and it is also the only avermectin approved in humans in several parasitic infestations. We examined this substance because potential critical issues associated with the use were highlighted. Moreover, criticisms related to the ecological-environmental sphere, as well as food contamination arise. Nevertheless, current in vitro studies are lacking. This study aimed to investigate the direct impact of IVM on reproductive function using swine granulosa cells as reproductive cell model. The results show that progesterone, ATP and free radicals’ levels significantly increased (p<0.05). Non-enzymatic defense systems as well as autophagy did not show significant differences. On the contrary, cell proliferation, estradiol levels, and enzymatic defense systems significantly decreased (p<0.05) after IVM treatment. Since results show that the normal function of these cells is compromised by IVM the normal reproductive functions of the entire organism could be disrupted.
伊维菌素(IVM)是兽医学中常用的抗寄生虫药物,也是在几种寄生虫感染中唯一被批准用于人类的阿维菌素。我们检查了这种物质,因为与使用相关的潜在关键问题得到了强调。此外,还出现了与生态环境领域以及食品污染有关的批评。然而,目前缺乏体外研究。本研究以猪颗粒细胞为生殖细胞模型,探讨IVM对猪生殖功能的直接影响。结果表明,黄体酮、ATP和自由基水平显著升高(p < 0.05)。非酶防御系统和自噬没有显著差异。相反,IVM处理后细胞增殖、雌二醇水平和酶防御系统显著降低(p < 0.05)。由于研究结果表明这些细胞的正常功能受到IVM的损害,整个生物体的正常生殖功能可能受到破坏。
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引用次数: 0
Maternal and paternal crude oil exposure differentially shapes early developmental transcriptomes and survival outcomes in Atlantic cod (Gadus morhua) 母本和父本原油暴露对大西洋鳕鱼早期发育转录组和生存结果的影响存在差异
IF 5.8 3区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES Pub Date : 2026-02-10 DOI: 10.1016/j.marpolbul.2026.119374
Claudia Erhart, Jasmine Nahrgang, Derrick Kwame Odei, Marianne Frantzen, Lisbet Sørensen, Mari Egeness Creese, Velmurugu Puvanendran, Øyvind Johannes Hansen, Bjørn Henrik Hansen, James P. Meador, Fekadu Yadetie
Understanding the long-term biological consequences of crude oil exposure on marine fish is essential for the sustainability of ecologically and economically important species such as Atlantic cod (Gadus morhua). While the direct effects of crude oil on early life stages are well documented, adult reproductive vulnerability and intergenerational consequences remain poorly understood, despite their pivotal role in spawning stock viability. Paternal contributions to next-generation outcomes are particularly underexplored. This study examined how parental exposure to a water-soluble fraction of crude oil affects transcriptomic profiles and survival outcomes in Atlantic cod offspring. Adult fish were exposed for 20 days prior to spawning, and offspring were produced by in vitro cross-fertilization to generate four groups: control (unexposed parents), maternally exposed, paternally exposed and biparentally exposed. Embryos were reared under control conditions, and transcriptome profiles were analyzed from fertilization to hatching, alongside assessments of cardiac function and morphology post-hatch. Offspring from exposed females failed to survive to hatching. Eggs were smaller, and transcriptomic data revealed severe downregulation of genes involved in early developmental processes. Chemical analyses confirmed maternal transfer of a diverse range of petroleum aromatic hydrocarbons to oocytes. Our findings point to disrupted oocyte provisioning, likely linked to endocrine and epigenetic disturbances during oocyte maturation. Paternal exposure had minimal effect on RNA expression, and morphology at hatching. However, reduced survival in early larval stages suggests sublethal effects emerging later, possibly through epigenetic mechanisms, a hypothesis requiring further investigation.
了解原油暴露对海洋鱼类的长期生物学影响,对于大西洋鳕鱼等重要生态和经济物种的可持续性至关重要。虽然原油对早期生命阶段的直接影响有充分的记录,但尽管它们在产卵种群的生存能力中起着关键作用,但对成年生殖脆弱性和代际后果的了解仍然很少。父亲对下一代的影响尤其没有得到充分的研究。本研究考察了亲代接触原油水溶性组分如何影响大西洋鳕鱼后代的转录组谱和生存结果。成鱼在产卵前暴露20天,通过体外受精产生后代,分为四组:对照组(未暴露的父母)、母暴露组、父暴露组和双父母暴露组。在对照条件下饲养胚胎,分析从受精到孵化的转录组谱,以及孵化后的心脏功能和形态学评估。受辐射的雌性的后代无法存活到孵化。卵子更小,转录组学数据显示参与早期发育过程的基因严重下调。化学分析证实母体将多种石油芳烃转移到卵母细胞。我们的研究结果表明,卵母细胞供应中断,可能与卵母细胞成熟过程中的内分泌和表观遗传干扰有关。父本暴露对RNA表达和孵化时的形态影响最小。然而,幼虫早期存活率降低表明亚致死效应出现较晚,可能是通过表观遗传机制,这一假设需要进一步研究。
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引用次数: 0
Corrigendum to "Temporo-spatial analysis of amyotrophic lateral sclerosis in Spain: Altitude and land use as new determinants of the disease" [Sci. Total Environ., 957 (2024), 177796]. 《西班牙肌萎缩性侧索硬化症的时空分析:海拔和土地利用是该病的新决定因素》的更正[Sci]。总环境。科学通报,957(2024),177796]。
IF 8 1区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES Pub Date : 2026-02-10 Epub Date: 2026-01-29 DOI: 10.1016/j.scitotenv.2026.181474
Ana Santurtún, Pablo Medín, José Antonio Riancho, Marina Santiago-Setién, Fernando Ortiz, Adolfo López de Munain, Ricardo Almendra, Javier Riancho
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引用次数: 0
Manganese removal in a full-scale constructed wetland for passive mine water treatment: environmental factors and microbial communities 矿井被动水处理全尺寸人工湿地除锰:环境因素和微生物群落
IF 12.8 1区 环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL Pub Date : 2026-02-10 DOI: 10.1016/j.watres.2026.125539
Charlotte Lafont, Stéphane Vaxelaire, Alexandre Gelabert, Catherine Joulian, Hugues Thouin, Frédéric Duré, Mickael Charron, Josselin Gorny, Delphine Vantelon, Fabienne Battaglia-Brunet, Éric D. van Hullebusch
Manganese (Mn) removal in passive mine water treatment remains a challenge due to its slow oxidation kinetics, requiring specific biogeochemical conditions. Constructed wetlands are often the key functional units enabling Mn removal in full-scale passive treatment plants. This study examines the key biogeochemical factors influencing Mn removal in a full-scale passive mine water treatment plant located in Alès (South-East France). Over one year, monitoring of physicochemical parameters, microbial communities, and Mn speciation in solid phases was conducted every two months. Results highlight temporal variations in Mn removal efficiency, with two main mechanisms identified: (1) Mn carbonate (MnCO₃) precipitation, likely influenced by high carbonate concentrations in mine water, and (2) Mn oxide (δ-MnO₂) formation, mainly associated with reed rhizosphere, where it accumulates as mineral plaque. In mine water, Mn removal correlates with Fe particle concentrations, suggesting a catalytic effect, as well as with alkalinity and the abundance of microorganisms affiliated to Alteromonadaceae, suggesting a microbial influence. Mn removal appears to be primarily abiotic, driven by favourable pH and alkaline conditions that promote Mn carbonate precipitation, by autocatalytic oxidation reactions occurring on rhizosphere surfaces and by plant’s design including surface area and hydrological conditions. Microbial communities may facilitate certain Mn removal processes depending on environmental conditions.
由于锰(Mn)的氧化动力学缓慢,需要特定的生物地球化学条件,因此被动矿山水处理中的锰(Mn)去除仍然是一个挑战。人工湿地通常是实现全规模被动处理厂除锰的关键功能单元。本研究考察了位于法国东南部al的一个大型被动矿山水处理厂中影响锰去除的关键生物地球化学因素。在一年多的时间里,每两个月进行一次理化参数、微生物群落和固相Mn形态的监测。结果突出了锰去除效率的时间变化,确定了两个主要机制:(1)碳酸锰(MnCO₃)降水,可能受到矿井水中高碳酸盐浓度的影响;(2)氧化锰(δ-MnO₂)形成,主要与芦苇根际有关,在那里它以矿物斑块的形式积累。在矿井水中,锰的去除与铁颗粒浓度相关,表明具有催化作用,还与碱度和Alteromonadaceae微生物的丰度相关,表明有微生物的影响。锰的去除似乎主要是非生物的,由促进碳酸锰沉淀的有利pH和碱性条件、根际表面发生的自催化氧化反应以及植物的设计(包括表面积和水文条件)驱动。微生物群落可以根据环境条件促进某些Mn的去除过程。
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引用次数: 0
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