环境科学与生态学最新文献

Cyantraniliprole (CYA), widely recognized as a highly effective solution, is widely used in pest management. It has been broadly utilized to manage diverse pests, among which Aphis gossypii Glover (Hemiptera: Aphididae) is a prominent agricultural pest that leads to significant crop damage worldwide. Studies suggest that the sublethal effect of insecticides might contribute to the resurgence of A. gossypii. Therefore, in this study, A. gossypii were exposed to sublethal doses of CYA (LC₁₅ and LC₃₀ values of 1.43 and 2.93 mg/L, respectively) for 48 h then employed life table parameters and RT-qPCR were used to estimate the sublethal and cross-generational impacts. Treatments with sublethal doses of CYA notably reduced the survival and reproduction of the F₀A. gossypii and CYA at LC₃₀ significantly increased the fecundity and population growth parameters (R₀, r, λ, and GRR) of F₁ and reduced in the pre-adult stage. Furthermore, gene expression analysis indicated a significant downregulation of juvenile hormone-binding protein (JHBP) in F₀. Conversely, the F₁ generation exhibited an upregulation of vitellogenin (Vg), insulin receptor substrate 1 (InS1), ecdysone receptor (EcR), and ultraspiracle protein (USP). The funding not only enhance the comprehension of the sublethal effects of CYA on A. gossypii but also provide valuable guidance for the effective utilization of insecticides in managing the pest.

Worldwide many crop varieties are considered seriously threatened while management strategies are still lacking. Goals and indicators relevant to the conservation of agrobiodiversity also remain to be developed. Here we present the results of three expert workshops in Peru designed to prioritise for conservation crops with high infra-specific diversity, along with the establishment of in-situ on-farm conservation goals and indicators. Analysing the results of a series of agrobiodiversity-related Payments for Ecosystem Services (PES) interventions realised between 2010 and 2021, "gaps" between current conservation status and the conservation goals are established. The identification of such gaps within the Peruvian regulatory framework is indispensable for justifying long-term government funding. Furthermore, the goal and indicator development processes initiated in Peru could also form a template for other countries, with a view to supporting the implementation of a range of international treaties, conventions and development goals.

Di(2-ethylhexyl) phthalate (DEHP) is a plasticizer used in various industrial and consumer products. It is not covalently bound within these products and leaches out during repeated use, heating, or cleaning. Main routes of environmental DEHP pollution are through the industrial and municipal wastewaters, which pollute aquatic environments. The reproductive system of adult fish is known to be vulnerable to DEHP exposure; however, the effects of early-life exposure to DEHP on reproductive function in adult zebrafish (Danio rerio) females are less studied. To evaluate the impact of early-life exposure to DEHP on freshwater female fish reproduction, zebrafish embryos were exposed to DEHP at 0, 10, 100, and 1000 nmol/L from 5 h post-fertilization (hpf) to 120 hpf (larval stage) and then raised to adulthood in clean water. DEHP decreased the number of released eggs and the fertilization rate after mating with unexposed males. Bodyweight and length, the weight of the ovaries, and the gonadosomatic index were decreased in adult female zebrafish following early-life exposure to DEHP. Histological analysis of the ovaries revealed that DEHP inhibited oogenesis. Serum 17β-estradiol levels were significantly reduced. DEHP inhibited gene expression of all three nuclear estrogen receptors in the ovaries, namely esr1, esr2a, and esr2b, and two gonadotropin receptors, fshr and lhr. These results suggest that transient early-life exposure to environmentally relevant concentrations of DEHP can inhibit the reproduction of adult female zebrafish.

Studies showed that contaminants adhered to the surface of nano-polystyrene microplastics (NPs) have a toxicological effect. Juveniles tilapia were dispersed into four groups: the control group A, 75 nm NPs exposed group B, 5 ng·L^-1 2,2',4,4',5,5'-hexabromodiphenyl ether group C (BDE₁₅₃), and 5 ng·L^-1 BDE₁₅₃ + 75 nm MPs group D, and acutely exposed for 2, 4 and 8 days. The hepatic histopathological change, enzymatic activities, transcriptomics, and proteomics, have been performed in tilapia. The results showed that the enzymatic activities of anti-oxidative (ROS, SOD, EROD), energy (ATP), lipid metabolism (TC, TG, FAS, LPL, ACC), pro-inflammatory (TNFα, IL-1β) and apoptosis (caspase 3) significantly increased at 2 d in BDE₁₅₃ and the combined group and together in BDE₁₅₃ group at 8 d. Histological slice showed displaced nucleus by BDE₁₅₃ exposure and vacuoles appeared in the combined groups. KEGG results revealed that pathways associated with endocytosis, protein processing in endoplasmic reticulum and regulation of actin cytoskeleton were significantly enriched. The selected genes associated with neurocentral development (ganab, diaph3/baiap2a/ddost decreased and increased), lipid metabolism (ldlrap1a decreased, stt3b increased), energy (agap2 decreased, uggt1 increased) were affected under co-exposure, and fibronectin significantly increased via proteome. Our study indicated that endocytosis, protein processing in endoplasmic reticulum, regulation of actin cytoskeleton were affected in tilapia liver under NPs and BDE₁₅₃ co-exposure.

The compound m-Cresol, also referred to as 3-methylphenol,acts as a precursor in the creation of pesticides and plasticizers. This research has conducted a thorough evaluation of the toxic effects of m-cresol on the cardiac development of juvenile zebrafish, from 6 to 72 hpf. The study's results reveal that higher concentrations of m-Cresol, compared to lower ones, result in more severe heart abnormalities in zebrafish larvae. The pericardial edema becomes more pronounced, the atrial-ventricular distance gradually increases, and the absorption of nutrients is delayed. Furthermore, experimental studies have shown that m-cresol can cause excessive oxidative stress and apoptosis in juvenile zebrafish during their early developmental stages. Additionally, our transcriptomic analysis indicates that m-Cresol exposure may cause cardiac developmental toxicity in zebrafish larvae by affecting the expression levels of genes (Myosin VIIa:my17,Myosin XIV:my14, Alpha-cardiac actin:actc1a,and Non-muscular myosin heavy chain 9 A:myh9a) involved in the ion channel signaling pathway and cardiomyocyte development. These findings collectively demonstrate the developmental toxicity of m-Cresol to the hearts of larval zebrafish.

Due to its high concentration and persistence, microplastic (MP) pollution is a major threat to marine environments. Expanded polystyrene (EPS) particles are the most abundant MP type in Asian regions, including the Korean coastal region. Although many previous studies have reported the toxicity of MPs to marine biota, the toxicity of environmentally relevant MPs to coastal organisms is not well understood. Thus, we investigated the toxicity of EPS on the growth and energy metabolism of the juvenile marine isopods, Ligia cinerascens, obtained from a population that has been exposed to EPS through multiple generations. After 14 and 21 days of dietary EPS exposure, body weight and molting of L. cinerascens were unaffected. However, the energy reserves (carbohydrates, proteins, and lipids) were significantly reduced, resulting in a decrease in the total energy budget (E_a) by dietary EPS exposure. The transcriptional modulation patterns of genes related to energy metabolism suggested that dietary EPS exposure may increase the digestion of non-carbohydrate sources, such as proteins and lipids, to compensate for increased energy expenditure. Our findings suggest that dietary EPS exposure, although no toxic at the individual level, can reduce the energy status of juvenile marine isopods, which provides useful information on the toxic effects of environmentally relevant MPs to coastal ecosystem.

The intricate interaction among host, pathogen, and environment significantly influences aquatic health, yet the influence of hypoxic stress combined with bacterial infection on host response is understudied. Circular RNAs with stable closed-loop structures have emerged as important regulators in immunity, yet remain ill-defined in fish. In this study, we systematically explored the circRNA response in yellow catfish subjected to combined hypoxia-bacterial infection (HB) stress. Following HB stress, H&E and TUNEL staining identified heightened hepatocyte apoptosis, intracellular vacuolation, and inflammatory tissue damage. RT-qPCR elucidated that differentially expressed genes stimulated by HB synergistically enhanced apoptosis and inflammatory responses. Importantly, we systematically evaluated differentially expressed circRNAs (DEcirs) in yellow catfish under hypoxia with and without Aeromonas veronii infection and identified a novel HB-specific DEcir, designated as circArid4b, whose parental gene Arid4b is highly associated with apoptosis. Experiments confirmed the circular structure of circArid4b and revealed that under HB stimulation, specific knockdown of circArid4b inhibited the expression of Arid4b, while concurrent alterations in multiple apoptosis- and inflammation-related genes synergistically indicated the promotion of apoptotic and inflammatory pathways. Notably, the downregulation of circArid4b expression significantly reduced the susceptibility to bacterial infection in yellow catfish during hypoxia. These results suggest that HB-induced suppression of circArid4b promotes cell apoptosis and inflammation by inhibiting its parental gene and thereby facilitating resistance to bacterial infection during hypoxia. Our study enriches the understanding of fish circRNA mechanisms and offers novel preventive and control strategies for bacterial infections in fish under hypoxic environments.

Olanzapine (OLZ) is widely used in the treatment of schizophrenia, and its metabolic side effects have garnered significant attention in recent years. Despite this, the specific side effects of OLZ and the underlying mechanisms remain inadequately understood. To address this gap, zebrafish (Danio rerio) were exposed to OLZ at concentrations of 35.5, 177.5, and 355.5 μg/L. The results indicated that exposure to OLZ significantly increased body weight, total cholesterol (TC), low-density lipoprotein (LDL), and triglycerides (TG). Histological analysis revealed notable lipid accumulation in the liver. Furthermore, lipid synthesis genes, including sterol regulatory element binding protein (srebp), acetyl CoA carboxylase (acc), and fatty acid synthesis gene (fas), were up-regulated. In contrast, genes related to lipid decomposition, such as lipoprotein lipase (lpl), hormone-sensitive triglyceride lipase (hsl), and carnitine palmitoyltransferase 1b (cpt1b), were down-regulated. Subsequent analysis of zebrafish behavior showed reduced motor activity, sociability, and anxiety-like behavior in OLZ-exposed zebrafish, consistent with the results of neurotransmitter related gene expression. Following OLZ treatment, the expression of tryptophan hydroxylase (tph), tyrosine hydroxylase (th), dopamine transporter (dat), glutaminase (glsa), and glutamic acid decarboxylase 1b (gad1b) was upregulated. Additionally, the diversity of intestinal flora decreased after OLZ exposure, and the structure of the intestinal microbiota changed significantly compared to the control group. At the genus level, the abundance of Plesiomonas was upregulated, while the abundances of Bacillus and Cetobacterium were downregulated in the OLZ-exposed group. Furthermore, the results of the correlation analysis indicated that lipid metabolism and behavioral changes were closely associated with the microbiota. This study clarified the side effects of OLZ, and also provided a basis for the reasonable discharge concentration of OLZ in water and clinical drug use.

Phytoplankton blooms create harmful toxins, scums, and taste and odor compounds and thus pose a major risk to drinking water safety. Climate and land use change are increasing the frequency and severity of blooms, motivating the development of new approaches for preemptive, rather than reactive, water management. While several real-time phytoplankton forecasts have been developed to date, none are both automated and quantify uncertainty in their predictions, which is critical for manager use. In response to this need, we outline a framework for developing the first automated, real-time lake phytoplankton forecasting system that quantifies uncertainty, thereby enabling managers to adapt operations and mitigate blooms. Implementation of this system calls for new, integrated ecosystem and statistical models; automated cyberinfrastructure; effective decision support tools; and training for forecasters and decision makers. We provide a research agenda for the creation of this system, as well as recommendations for developing real-time phytoplankton forecasts to support management.

The emergence of nature-based solutions (NbS) in science, policy, and practice signals a paradigmatic shift in urban climate change adaptation, yet empirical investigations into its impact on adaptation definitions and progress tracking remain scarce. Addressing this gap, we conducted thematic analysis on semi-structured interviews (n = 15) with practitioners responsible for implementing and evaluating urban NbS in different countries. We provide a nuanced understanding of urban adaptation goals within urban NbS according to the insights from these practitioners, extending beyond hazard mitigation and towards cultivating and strengthening relationships between humans and nature. Tracking adaptation progress towards such relational adaptation goals requires acknowledging knowledge pluralism and the diversity of human-nature relations. We propose an alternative definition of adaptation supported by our data that aims to foster a more holistic approach to urban climate adaptation that accounts for the potential benefits of urban NbS across interconnected climate, biodiversity, and social goals.